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The map label for this gene is pcaQ [H]

Identifier: 119385990

GI number: 119385990

Start: 436878

End: 438143

Strand: Direct

Name: pcaQ [H]

Synonym: Pden_3271

Alternate gene names: 119385990

Gene position: 436878-438143 (Clockwise)

Preceding gene: 119385988

Following gene: 119385993

Centisome position: 25.25

GC content: 62.8

Gene sequence:

>1266_bases
ATGTTTCGCCTCGCGGACATTCCGAACCTGCGCCATCTGCGCATGGTCCAGGTCATCGGCAAGCTCGGCGGGGTAAGCTG
CGCCTCGCGCGAATTGAGCACTTCGCAGCCAGCCGTGACCCAGGCCGTGGCCAATATCGAGGCCGAGATCGGGCTGCCCA
TCTTCGAACGTTGCGCTACCGGCACCTATCCCACCACGATCGGGAAGCAATTCCTGCTTCGTATCGACCGCTTCTTCGAT
ACGCTCGATACGGCGCTGGCACAGGTTCTCGGCCGGCCCGATGGTGCGGCGGACCGCATCCCGCCCCGGATAGAGCGGCT
TGCGACGGGAACCCAGCTCCGCGCGCTGATCGTGACCTGCGAGCAGGGCCGCGTCAGCGAGATCGCCGATTCACTGGGCC
TGTCGCCGGCATCGCTCTTCCGCTCGGCGCGGAGCTTCGAGCGAGCGCTCGGCAAGCAGCTTTTCGACCGGACGGCGCAG
GGTCCGATTCCGAACCGCACTGGAAACTACCTGGCCCGCGAATTCCGCCGCGCGGTGCGCGAATTGGAGCTGGCACGGGG
CGAGGTGCTGTTGGCCGCCGGGACCGAGAGTCTCGAGCTGGTCATAGGCACGCTTCCCATGGCGGGATCGCACGATCTGG
CCGAGGCGACGCGGCGGTTCATGTCCGCCTATCCCTCCACAAAGGTGCGGCTCGTCACCGGCGAATATCACAAGCTTCTC
AATGATCTTTCAAACAGCCGGATCGACATGATCTTCGGCATGCTGCGCCGTCCCGAATGGGCCGACGATGTAAGCGAGGA
AGTGCTTTTCACCGACAACTACTGTCTGGTTGCTCGTGTCGGGCACGCGTTGACGCGGCTTGAGAACGTCACCCCCTCGG
ATCTGGCTGGCTATGAATGGGTGGTCCCGCAGAAGGGCACGCCGCGGCGCAACCGGATCGAGCAGATCTTCGCAGATGAG
CCGGTGCGGCCGAAATTTCATCTCGAAACTTCGTCCCTGACGATGATCCGCTCGCTGTTGCTGAATTCAGAGACGGTTAC
CCTGATGACCCGGTCAGAGTTGCAGAACGATGTCGAGTTGGGCGTTCTCAAGGTTCTGCCCTGCAGGTTTCTTGACAATG
TGTTGCTGAAGGGCGTGACGACGCGCAGCGACTGGCTGCCGACCGCGGCGCATAACGCGTTTCTCGACTGCCTGCGCGAG
GTGACAGGAAAGACCAACCACGCGGGAAAGCCGGATTCGGGATCTGACATCCGAATGGCCTCCTGA

Upstream 100 bases:

>100_bases
CAGAGAGGAGGACGCCCGCCGGATCGCGGATCCAGCACGGGCGCGCCGGAGCGTGTTTCCGCAGCGCGGAATAGCCGCAT
CGAAGACAAGGGAGGAAAAC

Downstream 100 bases:

>100_bases
AGAGGCCTTATCGGAAGGCAAGGCCCGGAGCTTGTGCTCCGGGCCCCTTTTCACATCAGCGATTTCGCCTTTTCCAGCGT
GGCATAGCTGCGCGACGGAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Pca operon transcriptional activator [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MFRLADIPNLRHLRMVQVIGKLGGVSCASRELSTSQPAVTQAVANIEAEIGLPIFERCATGTYPTTIGKQFLLRIDRFFD
TLDTALAQVLGRPDGAADRIPPRIERLATGTQLRALIVTCEQGRVSEIADSLGLSPASLFRSARSFERALGKQLFDRTAQ
GPIPNRTGNYLAREFRRAVRELELARGEVLLAAGTESLELVIGTLPMAGSHDLAEATRRFMSAYPSTKVRLVTGEYHKLL
NDLSNSRIDMIFGMLRRPEWADDVSEEVLFTDNYCLVARVGHALTRLENVTPSDLAGYEWVVPQKGTPRRNRIEQIFADE
PVRPKFHLETSSLTMIRSLLLNSETVTLMTRSELQNDVELGVLKVLPCRFLDNVLLKGVTTRSDWLPTAAHNAFLDCLRE
VTGKTNHAGKPDSGSDIRMAS

Sequences:

>Translated_421_residues
MFRLADIPNLRHLRMVQVIGKLGGVSCASRELSTSQPAVTQAVANIEAEIGLPIFERCATGTYPTTIGKQFLLRIDRFFD
TLDTALAQVLGRPDGAADRIPPRIERLATGTQLRALIVTCEQGRVSEIADSLGLSPASLFRSARSFERALGKQLFDRTAQ
GPIPNRTGNYLAREFRRAVRELELARGEVLLAAGTESLELVIGTLPMAGSHDLAEATRRFMSAYPSTKVRLVTGEYHKLL
NDLSNSRIDMIFGMLRRPEWADDVSEEVLFTDNYCLVARVGHALTRLENVTPSDLAGYEWVVPQKGTPRRNRIEQIFADE
PVRPKFHLETSSLTMIRSLLLNSETVTLMTRSELQNDVELGVLKVLPCRFLDNVLLKGVTTRSDWLPTAAHNAFLDCLRE
VTGKTNHAGKPDSGSDIRMAS
>Mature_421_residues
MFRLADIPNLRHLRMVQVIGKLGGVSCASRELSTSQPAVTQAVANIEAEIGLPIFERCATGTYPTTIGKQFLLRIDRFFD
TLDTALAQVLGRPDGAADRIPPRIERLATGTQLRALIVTCEQGRVSEIADSLGLSPASLFRSARSFERALGKQLFDRTAQ
GPIPNRTGNYLAREFRRAVRELELARGEVLLAAGTESLELVIGTLPMAGSHDLAEATRRFMSAYPSTKVRLVTGEYHKLL
NDLSNSRIDMIFGMLRRPEWADDVSEEVLFTDNYCLVARVGHALTRLENVTPSDLAGYEWVVPQKGTPRRNRIEQIFADE
PVRPKFHLETSSLTMIRSLLLNSETVTLMTRSELQNDVELGVLKVLPCRFLDNVLLKGVTTRSDWLPTAAHNAFLDCLRE
VTGKTNHAGKPDSGSDIRMAS

Specific function: Activates transcription of the pcaDCHGB operon for the catabolism of the phenolic compound protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081904, Length=290, Percent_Identity=23.448275862069, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787601, Length=236, Percent_Identity=19.9152542372881, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790399, Length=257, Percent_Identity=22.9571984435798, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR012787
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 46656; Mature: 46656

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRLADIPNLRHLRMVQVIGKLGGVSCASRELSTSQPAVTQAVANIEAEIGLPIFERCAT
CCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCC
GTYPTTIGKQFLLRIDRFFDTLDTALAQVLGRPDGAADRIPPRIERLATGTQLRALIVTC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHEEEEEEEE
EQGRVSEIADSLGLSPASLFRSARSFERALGKQLFDRTAQGPIPNRTGNYLAREFRRAVR
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
ELELARGEVLLAAGTESLELVIGTLPMAGSHDLAEATRRFMSAYPSTKVRLVTGEYHKLL
HHHHHCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHH
NDLSNSRIDMIFGMLRRPEWADDVSEEVLFTDNYCLVARVGHALTRLENVTPSDLAGYEW
HHHCCCHHHHHHHHHCCCCCCHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHCCCEE
VVPQKGTPRRNRIEQIFADEPVRPKFHLETSSLTMIRSLLLNSETVTLMTRSELQNDVEL
ECCCCCCCHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHH
GVLKVLPCRFLDNVLLKGVTTRSDWLPTAAHNAFLDCLREVTGKTNHAGKPDSGSDIRMA
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
S
C
>Mature Secondary Structure
MFRLADIPNLRHLRMVQVIGKLGGVSCASRELSTSQPAVTQAVANIEAEIGLPIFERCAT
CCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCC
GTYPTTIGKQFLLRIDRFFDTLDTALAQVLGRPDGAADRIPPRIERLATGTQLRALIVTC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHEEEEEEEE
EQGRVSEIADSLGLSPASLFRSARSFERALGKQLFDRTAQGPIPNRTGNYLAREFRRAVR
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
ELELARGEVLLAAGTESLELVIGTLPMAGSHDLAEATRRFMSAYPSTKVRLVTGEYHKLL
HHHHHCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHH
NDLSNSRIDMIFGMLRRPEWADDVSEEVLFTDNYCLVARVGHALTRLENVTPSDLAGYEW
HHHCCCHHHHHHHHHCCCCCCHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHCCCEE
VVPQKGTPRRNRIEQIFADEPVRPKFHLETSSLTMIRSLLLNSETVTLMTRSELQNDVEL
ECCCCCCCHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHH
GVLKVLPCRFLDNVLLKGVTTRSDWLPTAAHNAFLDCLREVTGKTNHAGKPDSGSDIRMA
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]