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The map label for this gene is hupD [H]

Identifier: 119385823

GI number: 119385823

Start: 287636

End: 288304

Strand: Direct

Name: hupD [H]

Synonym: Pden_3101

Alternate gene names: 119385823

Gene position: 287636-288304 (Clockwise)

Preceding gene: 119385822

Following gene: 119385824

Centisome position: 16.63

GC content: 66.52

Gene sequence:

>669_bases
ATGACCGCACCGCCTGCCAGCAATGCTTCGGGGAGTTCGGGGAGGGGGCAGATGCCAGCGGAAGCGCCTGCAAATATCCT
TGTTCTGGGGATCGGCAATGTGCTTTGGGCCGATGAGGGCTTCGGGGTGCGCTGCGTCGAAGAGATGGCCGCGCGCCATG
CCTTGCCCGAACGGGTGAGGCTGCTTGATGGCGGCACGCAGGGGCTGTATCTGCTGCCCTTTCTGGAAGAAGCCGACGCG
CTGATCGTCTTTGACGCGGTGGATTACGGACTGCCCCCCGGCACGCTGAAGCTGGTCGAAGGGGCCGAGGTTCCGGCCTT
TCTGGGCGCCAAAAAAATGAGCCTGCACCAGACCGGCTTTCAAGAGGTGATCGCGACCGCCCAGCTGCTGGGGCGTTGTC
CGGCGCAGCTGATGCTGATCGGCTGCCAGCCGGTCGAACTGGAGGATTACGGAGGAGGACTCCGCCCCGAAGTGGCTGCA
CAGATCGGGCCTGCCATCGAGATTGCGCTGGCGAAGCTGGCCGAATGGTGCGTTCAGGCCGGCGAGGGCGCGACCTCGGG
TCAGCTTGCGGATCCGGCGATCCGGCGCGAGGCCTATGAGACGGGCCGCCCCGCCGCCTCTGATGCCTGCCGCGAGGGCG
ATGCGCGCTTTTTCCCGGGGGCCGGCTGA

Upstream 100 bases:

>100_bases
TTTTTATATCATTATCTTGCAAGGAATTTCGAGGGTTGCGACTTTTTGTCTCTGCTTTCGGAACTGGTGGCTATACTTTC
GGCCCATAGGGGTGGGGCAC

Downstream 100 bases:

>100_bases
TGTGCATGGGCATCCCGATGCGGCTGGCGCAGGTCGACGGCATCGCGGGGCAAAGCGAGGGCGGCGATCTGATCGACCTC
AGCCTGACCGCCGATGCGCA

Product: hydrogenase expression/formation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MTAPPASNASGSSGRGQMPAEAPANILVLGIGNVLWADEGFGVRCVEEMAARHALPERVRLLDGGTQGLYLLPFLEEADA
LIVFDAVDYGLPPGTLKLVEGAEVPAFLGAKKMSLHQTGFQEVIATAQLLGRCPAQLMLIGCQPVELEDYGGGLRPEVAA
QIGPAIEIALAKLAEWCVQAGEGATSGQLADPAIRREAYETGRPAASDACREGDARFFPGAG

Sequences:

>Translated_222_residues
MTAPPASNASGSSGRGQMPAEAPANILVLGIGNVLWADEGFGVRCVEEMAARHALPERVRLLDGGTQGLYLLPFLEEADA
LIVFDAVDYGLPPGTLKLVEGAEVPAFLGAKKMSLHQTGFQEVIATAQLLGRCPAQLMLIGCQPVELEDYGGGLRPEVAA
QIGPAIEIALAKLAEWCVQAGEGATSGQLADPAIRREAYETGRPAASDACREGDARFFPGAG
>Mature_221_residues
TAPPASNASGSSGRGQMPAEAPANILVLGIGNVLWADEGFGVRCVEEMAARHALPERVRLLDGGTQGLYLLPFLEEADAL
IVFDAVDYGLPPGTLKLVEGAEVPAFLGAKKMSLHQTGFQEVIATAQLLGRCPAQLMLIGCQPVELEDYGGGLRPEVAAQ
IGPAIEIALAKLAEWCVQAGEGATSGQLADPAIRREAYETGRPAASDACREGDARFFPGAG

Specific function: Not known. Could be involved in the processing of hydrogenase [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1787209, Length=129, Percent_Identity=55.0387596899225, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1789367, Length=156, Percent_Identity=44.8717948717949, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 23112; Mature: 22980

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPPASNASGSSGRGQMPAEAPANILVLGIGNVLWADEGFGVRCVEEMAARHALPERVR
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCHHHHHHHHHHHCCHHHHH
LLDGGTQGLYLLPFLEEADALIVFDAVDYGLPPGTLKLVEGAEVPAFLGAKKMSLHQTGF
HHCCCCCCEEEEEEECCCCEEEEEECHHCCCCCCCEEEECCCCCCHHHCCHHHHHHHHHH
QEVIATAQLLGRCPAQLMLIGCQPVELEDYGGGLRPEVAAQIGPAIEIALAKLAEWCVQA
HHHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHC
GEGATSGQLADPAIRREAYETGRPAASDACREGDARFFPGAG
CCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TAPPASNASGSSGRGQMPAEAPANILVLGIGNVLWADEGFGVRCVEEMAARHALPERVR
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCHHHHHHHHHHHCCHHHHH
LLDGGTQGLYLLPFLEEADALIVFDAVDYGLPPGTLKLVEGAEVPAFLGAKKMSLHQTGF
HHCCCCCCEEEEEEECCCCEEEEEECHHCCCCCCCEEEECCCCCCHHHCCHHHHHHHHHH
QEVIATAQLLGRCPAQLMLIGCQPVELEDYGGGLRPEVAAQIGPAIEIALAKLAEWCVQA
HHHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHC
GEGATSGQLADPAIRREAYETGRPAASDACREGDARFFPGAG
CCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497190 [H]