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The map label for this gene is hupB [H]

Identifier: 119385820

GI number: 119385820

Start: 284286

End: 286079

Strand: Direct

Name: hupB [H]

Synonym: Pden_3098

Alternate gene names: 119385820

Gene position: 284286-286079 (Clockwise)

Preceding gene: 119385819

Following gene: 119385821

Centisome position: 16.43

GC content: 60.26

Gene sequence:

>1794_bases
ATGACGATCAACACGCCGAATGGCTATACCCTCGACAATTCCGGGCGCCGCATCGTGGTGGACCCGGTCACCCGGATCGA
AGGGCATATGCGCTGCGAGGTCAACGTGGATGATCAGGGTGTCATCCGCAACGCGGTCTCGACCGGGACGATGTGGCGCG
GGCTGGAGGTCATCCTGAAAGGGCGCGACCCGCGCGACGCCTGGGCCTTTGTCGAGCGGATCTGCGGCGTCTGCACTGGC
ACGCATGCGCTGACCTCGGTGCGCGCGGTCGAGGATGCGCTTGGGATCATGATCCCCGACAATGCCAACTCGATCCGCAA
CATGATGCAGCTGGCGCTGGAGATCCACGATCATATCGTGCATTTCTATCACCTGCATGCGCTGGACTGGGTGAATCCGG
TCAATGCCCTGCGCGCCGACCCCAAGGCGACCAGCGAATTGCAGCAGATGGTCTCGCCCTCGCATCCGATGTCATCGCCG
GGCTATTTCCGCGATGTGCAGAACCGGCTGAAGAAATTCGTCGAATCCGGGCAACTGGGGCTGTTCAAGAACGGCTATTG
GGACAATCCGGCCTATCTTCTGCCGCCCGAAGCCGATCTGATGGCGACGACTCACTATCTGGAGGCGCTTGATCTGCAAA
AGGAAATCGTCAAGGTCCATACGATCTTTGGCGGCAAGAACCCGCATCCGAACTGGCTGGTGGGCGGGGTGCCATGCCCG
ATCAATATCGACGGCACCGGATCTGTCGGCGCGATCAATATGGAGCGGCTGAACCTCGTCTCCTCGATCATTGATCTGTG
CGACGAGTTCAACAAGAACGTCTATATTCCCGATGTGATCGCCATCGGCGGCTTTTACAAAAGCTGGCTTTACGGGGGCG
GGCTGTCGTCGCAGGCGGTCATGGCCTATGGCGACATTCCCGAGAACCCGAATGACTTTTCGCCCGAGCAACTGCATTTG
CCGCGCGGCGCGATCATCAACGGCAATCTCAACGAGGTGCATGACGTCGATCCGCGCGACCCCGAGCAGGTGCAGGAATT
CGTCGACCATTCCTGGTATTCGTATGGCGATCCGGGCCGCGGCCTGCATCCCTGGGATGGTGAAACCAATCCGAAATTCG
AGCTGGGGCCGAACCACAAGGGCACGAAAACTCGCATCGAACAGCTGGATGAATCCGCGAAATATTCCTGGATCAAGGCG
CCGCGCTGGCGAGGTCACGCGATGGAGGTCGGCCCGCTTGCGCGCTATATCATCGGCTACGCCAAGGGGCATGAGGACAT
CAAAAATCAGGTCGATGGCCTGTTGAGAACCATGGATTTGCCCTTCGAGGCGCTGTTCTCGACTCTGGGCCGCACGGCGG
CGCGGGCGCTGGAATCGGAATATTGCGGGCGCCTGCAACGGCATTTCTTCGACAAGATGGTGCGGAACATCAAGAATGGC
GATCAAAGCACCGCCAATGTCGAGAAATGGGATCCTTCGACCTGGCCGAAAGAGGCCAAGGGCGTCGGCATGACCGAGGC
ACCGCGCGGCGCGCTTGGCCATTGGATCCGCATCAAGGACGGCCGGATCGAGAATTACCAATGCGTGGTGCCGACCACCT
GGAATGGCTCGCCCCGCGACAGCCAGGGCAATATCGGTGCCTTCGAGGCCAGCCTGATGAACACGCCCATGGAGCGCCCG
GATGAGCCGGTCGAAATCCTGCGCACGCTGCACAGTTTCGACCCCTGCCTGGCCTGCTCTACCCATGTCATGTCACCGGA
TGGTGACGAGCTTGCCGCCGTCAAAATTCGCTGA

Upstream 100 bases:

>100_bases
CGCGGCGGGTGCAGTCGGTGGGGCCGTCGCGGTCCATGCCGCGATTTCGGCGCTGAAACGGGCGCAGAAGAAGACCCAGG
ACAAGGTGGAGGCCTAGGCC

Downstream 100 bases:

>100_bases
GAAAGGGACGATCCAATGAAAAAACTGATGCTGGCCGCGCTGGCCGCACTCATGGGCTCACCCGTTCTGGCCCATACCGG
GGTGGGAGAGACCTCGGGCT

Product: nickel-dependent hydrogenase, large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MTINTPNGYTLDNSGRRIVVDPVTRIEGHMRCEVNVDDQGVIRNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTG
THALTSVRAVEDALGIMIPDNANSIRNMMQLALEIHDHIVHFYHLHALDWVNPVNALRADPKATSELQQMVSPSHPMSSP
GYFRDVQNRLKKFVESGQLGLFKNGYWDNPAYLLPPEADLMATTHYLEALDLQKEIVKVHTIFGGKNPHPNWLVGGVPCP
INIDGTGSVGAINMERLNLVSSIIDLCDEFNKNVYIPDVIAIGGFYKSWLYGGGLSSQAVMAYGDIPENPNDFSPEQLHL
PRGAIINGNLNEVHDVDPRDPEQVQEFVDHSWYSYGDPGRGLHPWDGETNPKFELGPNHKGTKTRIEQLDESAKYSWIKA
PRWRGHAMEVGPLARYIIGYAKGHEDIKNQVDGLLRTMDLPFEALFSTLGRTAARALESEYCGRLQRHFFDKMVRNIKNG
DQSTANVEKWDPSTWPKEAKGVGMTEAPRGALGHWIRIKDGRIENYQCVVPTTWNGSPRDSQGNIGAFEASLMNTPMERP
DEPVEILRTLHSFDPCLACSTHVMSPDGDELAAVKIR

Sequences:

>Translated_597_residues
MTINTPNGYTLDNSGRRIVVDPVTRIEGHMRCEVNVDDQGVIRNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTG
THALTSVRAVEDALGIMIPDNANSIRNMMQLALEIHDHIVHFYHLHALDWVNPVNALRADPKATSELQQMVSPSHPMSSP
GYFRDVQNRLKKFVESGQLGLFKNGYWDNPAYLLPPEADLMATTHYLEALDLQKEIVKVHTIFGGKNPHPNWLVGGVPCP
INIDGTGSVGAINMERLNLVSSIIDLCDEFNKNVYIPDVIAIGGFYKSWLYGGGLSSQAVMAYGDIPENPNDFSPEQLHL
PRGAIINGNLNEVHDVDPRDPEQVQEFVDHSWYSYGDPGRGLHPWDGETNPKFELGPNHKGTKTRIEQLDESAKYSWIKA
PRWRGHAMEVGPLARYIIGYAKGHEDIKNQVDGLLRTMDLPFEALFSTLGRTAARALESEYCGRLQRHFFDKMVRNIKNG
DQSTANVEKWDPSTWPKEAKGVGMTEAPRGALGHWIRIKDGRIENYQCVVPTTWNGSPRDSQGNIGAFEASLMNTPMERP
DEPVEILRTLHSFDPCLACSTHVMSPDGDELAAVKIR
>Mature_596_residues
TINTPNGYTLDNSGRRIVVDPVTRIEGHMRCEVNVDDQGVIRNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTGT
HALTSVRAVEDALGIMIPDNANSIRNMMQLALEIHDHIVHFYHLHALDWVNPVNALRADPKATSELQQMVSPSHPMSSPG
YFRDVQNRLKKFVESGQLGLFKNGYWDNPAYLLPPEADLMATTHYLEALDLQKEIVKVHTIFGGKNPHPNWLVGGVPCPI
NIDGTGSVGAINMERLNLVSSIIDLCDEFNKNVYIPDVIAIGGFYKSWLYGGGLSSQAVMAYGDIPENPNDFSPEQLHLP
RGAIINGNLNEVHDVDPRDPEQVQEFVDHSWYSYGDPGRGLHPWDGETNPKFELGPNHKGTKTRIEQLDESAKYSWIKAP
RWRGHAMEVGPLARYIIGYAKGHEDIKNQVDGLLRTMDLPFEALFSTLGRTAARALESEYCGRLQRHFFDKMVRNIKNGD
QSTANVEKWDPSTWPKEAKGVGMTEAPRGALGHWIRIKDGRIENYQCVVPTTWNGSPRDSQGNIGAFEASLMNTPMERPD
EPVEILRTLHSFDPCLACSTHVMSPDGDELAAVKIR

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=591, Percent_Identity=62.9441624365482, Blast_Score=789, Evalue=0.0,
Organism=Escherichia coli, GI1789368, Length=595, Percent_Identity=44.0336134453782, Blast_Score=488, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 66672; Mature: 66541

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTINTPNGYTLDNSGRRIVVDPVTRIEGHMRCEVNVDDQGVIRNAVSTGTMWRGLEVILK
CCCCCCCCEEECCCCCEEEECCHHHCCCCEEEEEECCCCHHHHHHHHHCHHHHCHHHEEE
GRDPRDAWAFVERICGVCTGTHALTSVRAVEDALGIMIPDNANSIRNMMQLALEIHDHIV
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHH
HFYHLHALDWVNPVNALRADPKATSELQQMVSPSHPMSSPGYFRDVQNRLKKFVESGQLG
HHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCE
LFKNGYWDNPAYLLPPEADLMATTHYLEALDLQKEIVKVHTIFGGKNPHPNWLVGGVPCP
EEECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCE
INIDGTGSVGAINMERLNLVSSIIDLCDEFNKNVYIPDVIAIGGFYKSWLYGGGLSSQAV
EEECCCCCCCEEHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHCCCCCCCEE
MAYGDIPENPNDFSPEQLHLPRGAIINGNLNEVHDVDPRDPEQVQEFVDHSWYSYGDPGR
EEECCCCCCCCCCCCCCEECCCCEEECCCCHHCCCCCCCCHHHHHHHHHCCCCCCCCCCC
GLHPWDGETNPKFELGPNHKGTKTRIEQLDESAKYSWIKAPRWRGHAMEVGPLARYIIGY
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHH
AKGHEDIKNQVDGLLRTMDLPFEALFSTLGRTAARALESEYCGRLQRHFFDKMVRNIKNG
HCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DQSTANVEKWDPSTWPKEAKGVGMTEAPRGALGHWIRIKDGRIENYQCVVPTTWNGSPRD
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCC
SQGNIGAFEASLMNTPMERPDEPVEILRTLHSFDPCLACSTHVMSPDGDELAAVKIR
CCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEEC
>Mature Secondary Structure 
TINTPNGYTLDNSGRRIVVDPVTRIEGHMRCEVNVDDQGVIRNAVSTGTMWRGLEVILK
CCCCCCCEEECCCCCEEEECCHHHCCCCEEEEEECCCCHHHHHHHHHCHHHHCHHHEEE
GRDPRDAWAFVERICGVCTGTHALTSVRAVEDALGIMIPDNANSIRNMMQLALEIHDHIV
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHH
HFYHLHALDWVNPVNALRADPKATSELQQMVSPSHPMSSPGYFRDVQNRLKKFVESGQLG
HHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCE
LFKNGYWDNPAYLLPPEADLMATTHYLEALDLQKEIVKVHTIFGGKNPHPNWLVGGVPCP
EEECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCE
INIDGTGSVGAINMERLNLVSSIIDLCDEFNKNVYIPDVIAIGGFYKSWLYGGGLSSQAV
EEECCCCCCCEEHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHCCCCCCCEE
MAYGDIPENPNDFSPEQLHLPRGAIINGNLNEVHDVDPRDPEQVQEFVDHSWYSYGDPGR
EEECCCCCCCCCCCCCCEECCCCEEECCCCHHCCCCCCCCHHHHHHHHHCCCCCCCCCCC
GLHPWDGETNPKFELGPNHKGTKTRIEQLDESAKYSWIKAPRWRGHAMEVGPLARYIIGY
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHH
AKGHEDIKNQVDGLLRTMDLPFEALFSTLGRTAARALESEYCGRLQRHFFDKMVRNIKNG
HCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DQSTANVEKWDPSTWPKEAKGVGMTEAPRGALGHWIRIKDGRIENYQCVVPTTWNGSPRD
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCC
SQGNIGAFEASLMNTPMERPDEPVEILRTLHSFDPCLACSTHVMSPDGDELAAVKIR
CCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3067084 [H]