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The map label for this gene is nylA [H]

Identifier: 119385775

GI number: 119385775

Start: 232485

End: 233912

Strand: Direct

Name: nylA [H]

Synonym: Pden_3053

Alternate gene names: 119385775

Gene position: 232485-233912 (Clockwise)

Preceding gene: 119385774

Following gene: 119385776

Centisome position: 13.44

GC content: 65.62

Gene sequence:

>1428_bases
ATGATCAGTGACATTCTTGATCTCGATGCGACAGCGCAGGCTGCGGCCGTCGCGAAGGGCGATGTCAGCGCGGCAGAGCT
GGCCCGCGCCGCGATTGCGAGGATCGAGGCCGGGGAGCCCGTGCTCAATGCGGTGACGCAGCGCTTTTTCGATCAGGCGC
TCGATATGGCATCAGCCGATCTGCCGCCGGGGCCGTTTCGCGGCGTGCCCTTTCTGGTGAAGGACTTTTACTGCCATATG
GCGGGAACCCCGACCACCGGCAGCGCAAAGCTGCTGGAGCACAACTTGATCGACCATGACAGCGCACTGATGGCTCGCTA
TCGCCAGGCGGGGTTTGTGACGCTCGGCAAGACCAATGTGCCGGAAATGGTCACCATGGGCACGACCGAGCCGGTCTGGC
GCGGCCCCACGCGTAACCCCTGGAATACCGGTCATACGCCGGGCGGGTCTTCGGGTGGCTCGGCGGCGGCGGTCGCGGCG
GGATATGTCGCGGTGGCCCATGCCAATGATGGCGCCGGATCCATCCGCATCCCGGCCTCATGCTGCGGTCTCTTCGGGCT
GAAGCCTTCGCGGGCCCGGGTCACGCTCGGGCCGGATGTTGGCGAGGCCATCGGTGGCATCACGGCGGAACATGTGGTTT
CGCGATCCGTGCGGGACAGTGCGGCGGTGCTCGACGCCACCGCCGGAGCCCTGCCGGGAGACCCCTATATCGCCCCCGAG
CCGCCGGAAGGCTTCCTTGCCGCCATCGCCAGACCGCGCAAGGGGCTGCGGATCGGCCTGACGCTCGATGCGCCGGACGG
CACCAGCGCCGCGCCGGAATGTCAAGCTGCGGCTCTGCGGGCGGCGGTGCTCTTGCGTGATCTTGGTCATCAGGTCGAGG
AGGTCACGTTGCCCTTCGACGGCGCCGCCTTCCGCGCGGCGCTCTCCACCTTCTGGCCGATGACCGTGACCCGGGGCCTC
AGCGCGATTGCGGAGGCCCGGGACGCGGACCCCGAAGTTCTGTTGCGCGAGCTTGAGCCCTTTAACCAGCATCTCTTCGC
TCTTGGGATCTCGCGCCGGGCGGTAGATTACATCCGGGATCTTGTGATCTTTCAGGGAATGGCGCGGGCCATGGGGCAGT
TCTTTGCCACACAGGATATCTGGCTTTCGCCGGTCCTGCCCTTCACGCCGCCCCGGCTTGGCTATTTTGATGCGGGTGTC
CTGGGCGGGGAAACGGCCTGGAACCGGGTGCTCGACTCCTTCATGTTCACTGCGCCCGCCAATGTCACCGGCCTGCCTTC
TGCCAGCTTGCCGATGACCGTGTCGAATGAGGGGCTTCCCATAGGCATCCAGTTGACGGCGAAGCTGAATGACGAAGCGC
TCATCTTCAACCTCGCAGCGCAGCTTGAAGCCGCAAACCCCTGGGCGATGCCTAGGCACAAGATCTAA

Upstream 100 bases:

>100_bases
ATCGTCACGACAGCCGAACTGGAGCTACTTTTCAACCGCCTGCGACAGGCGCTGGATGCTCTGGCCCTTGAAACCGCCGC
GAGCCAGGTGGAGGACCGGC

Downstream 100 bases:

>100_bases
GCAAAAACCAAACACAACAGGGAGTTACAACATGGGTCATCGCATCCATCGGAGAGGCATGCTTAAAGGGTCGGCCTGGC
TGGCCGGGGCTGCCGCAGTC

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MISDILDLDATAQAAAVAKGDVSAAELARAAIARIEAGEPVLNAVTQRFFDQALDMASADLPPGPFRGVPFLVKDFYCHM
AGTPTTGSAKLLEHNLIDHDSALMARYRQAGFVTLGKTNVPEMVTMGTTEPVWRGPTRNPWNTGHTPGGSSGGSAAAVAA
GYVAVAHANDGAGSIRIPASCCGLFGLKPSRARVTLGPDVGEAIGGITAEHVVSRSVRDSAAVLDATAGALPGDPYIAPE
PPEGFLAAIARPRKGLRIGLTLDAPDGTSAAPECQAAALRAAVLLRDLGHQVEEVTLPFDGAAFRAALSTFWPMTVTRGL
SAIAEARDADPEVLLRELEPFNQHLFALGISRRAVDYIRDLVIFQGMARAMGQFFATQDIWLSPVLPFTPPRLGYFDAGV
LGGETAWNRVLDSFMFTAPANVTGLPSASLPMTVSNEGLPIGIQLTAKLNDEALIFNLAAQLEAANPWAMPRHKI

Sequences:

>Translated_475_residues
MISDILDLDATAQAAAVAKGDVSAAELARAAIARIEAGEPVLNAVTQRFFDQALDMASADLPPGPFRGVPFLVKDFYCHM
AGTPTTGSAKLLEHNLIDHDSALMARYRQAGFVTLGKTNVPEMVTMGTTEPVWRGPTRNPWNTGHTPGGSSGGSAAAVAA
GYVAVAHANDGAGSIRIPASCCGLFGLKPSRARVTLGPDVGEAIGGITAEHVVSRSVRDSAAVLDATAGALPGDPYIAPE
PPEGFLAAIARPRKGLRIGLTLDAPDGTSAAPECQAAALRAAVLLRDLGHQVEEVTLPFDGAAFRAALSTFWPMTVTRGL
SAIAEARDADPEVLLRELEPFNQHLFALGISRRAVDYIRDLVIFQGMARAMGQFFATQDIWLSPVLPFTPPRLGYFDAGV
LGGETAWNRVLDSFMFTAPANVTGLPSASLPMTVSNEGLPIGIQLTAKLNDEALIFNLAAQLEAANPWAMPRHKI
>Mature_475_residues
MISDILDLDATAQAAAVAKGDVSAAELARAAIARIEAGEPVLNAVTQRFFDQALDMASADLPPGPFRGVPFLVKDFYCHM
AGTPTTGSAKLLEHNLIDHDSALMARYRQAGFVTLGKTNVPEMVTMGTTEPVWRGPTRNPWNTGHTPGGSSGGSAAAVAA
GYVAVAHANDGAGSIRIPASCCGLFGLKPSRARVTLGPDVGEAIGGITAEHVVSRSVRDSAAVLDATAGALPGDPYIAPE
PPEGFLAAIARPRKGLRIGLTLDAPDGTSAAPECQAAALRAAVLLRDLGHQVEEVTLPFDGAAFRAALSTFWPMTVTRGL
SAIAEARDADPEVLLRELEPFNQHLFALGISRRAVDYIRDLVIFQGMARAMGQFFATQDIWLSPVLPFTPPRLGYFDAGV
LGGETAWNRVLDSFMFTAPANVTGLPSASLPMTVSNEGLPIGIQLTAKLNDEALIFNLAAQLEAANPWAMPRHKI

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=508, Percent_Identity=25, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI195972892, Length=245, Percent_Identity=27.7551020408163, Blast_Score=86, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=272, Percent_Identity=31.6176470588235, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=441, Percent_Identity=26.530612244898, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=311, Percent_Identity=28.6173633440514, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=219, Percent_Identity=28.7671232876712, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=308, Percent_Identity=30.1948051948052, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=302, Percent_Identity=27.8145695364238, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=308, Percent_Identity=30.8441558441558, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24644968, Length=494, Percent_Identity=24.6963562753036, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24652985, Length=524, Percent_Identity=25.5725190839695, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19922090, Length=524, Percent_Identity=25.5725190839695, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24652981, Length=524, Percent_Identity=25.5725190839695, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24652983, Length=524, Percent_Identity=25.5725190839695, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648113, Length=448, Percent_Identity=26.5625, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI161078093, Length=505, Percent_Identity=24.7524752475248, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21356731, Length=227, Percent_Identity=30.8370044052863, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI45550774, Length=202, Percent_Identity=27.7227722772277, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24648435, Length=202, Percent_Identity=27.7227722772277, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648437, Length=202, Percent_Identity=27.7227722772277, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 49946; Mature: 49946

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISDILDLDATAQAAAVAKGDVSAAELARAAIARIEAGEPVLNAVTQRFFDQALDMASAD
CCCCHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
LPPGPFRGVPFLVKDFYCHMAGTPTTGSAKLLEHNLIDHDSALMARYRQAGFVTLGKTNV
CCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCEEEECCCCC
PEMVTMGTTEPVWRGPTRNPWNTGHTPGGSSGGSAAAVAAGYVAVAHANDGAGSIRIPAS
CCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCEEEEEECCCCCCEEECCHH
CCGLFGLKPSRARVTLGPDVGEAIGGITAEHVVSRSVRDSAAVLDATAGALPGDPYIAPE
HHHHCCCCCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PPEGFLAAIARPRKGLRIGLTLDAPDGTSAAPECQAAALRAAVLLRDLGHQVEEVTLPFD
CCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHEEECCCC
GAAFRAALSTFWPMTVTRGLSAIAEARDADPEVLLRELEPFNQHLFALGISRRAVDYIRD
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
LVIFQGMARAMGQFFATQDIWLSPVLPFTPPRLGYFDAGVLGGETAWNRVLDSFMFTAPA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
NVTGLPSASLPMTVSNEGLPIGIQLTAKLNDEALIFNLAAQLEAANPWAMPRHKI
CCCCCCCCCCCEEECCCCCEEEEEEEEECCCCEEEEEHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MISDILDLDATAQAAAVAKGDVSAAELARAAIARIEAGEPVLNAVTQRFFDQALDMASAD
CCCCHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
LPPGPFRGVPFLVKDFYCHMAGTPTTGSAKLLEHNLIDHDSALMARYRQAGFVTLGKTNV
CCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCEEEECCCCC
PEMVTMGTTEPVWRGPTRNPWNTGHTPGGSSGGSAAAVAAGYVAVAHANDGAGSIRIPAS
CCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCEEEEEECCCCCCEEECCHH
CCGLFGLKPSRARVTLGPDVGEAIGGITAEHVVSRSVRDSAAVLDATAGALPGDPYIAPE
HHHHCCCCCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PPEGFLAAIARPRKGLRIGLTLDAPDGTSAAPECQAAALRAAVLLRDLGHQVEEVTLPFD
CCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHEEECCCC
GAAFRAALSTFWPMTVTRGLSAIAEARDADPEVLLRELEPFNQHLFALGISRRAVDYIRD
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
LVIFQGMARAMGQFFATQDIWLSPVLPFTPPRLGYFDAGVLGGETAWNRVLDSFMFTAPA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
NVTGLPSASLPMTVSNEGLPIGIQLTAKLNDEALIFNLAAQLEAANPWAMPRHKI
CCCCCCCCCCCEEECCCCCEEEEEEEEECCCCEEEEEHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]