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The map label for this gene is bioA [C]

Identifier: 119385774

GI number: 119385774

Start: 231104

End: 232495

Strand: Direct

Name: bioA [C]

Synonym: Pden_3052

Alternate gene names: 119385774

Gene position: 231104-232495 (Clockwise)

Preceding gene: 119385773

Following gene: 119385775

Centisome position: 13.36

GC content: 64.01

Gene sequence:

>1392_bases
ATGATGCGCAATTCTCTGGCCCATACCGATCTGAACTGCCTGCTGCATCCCCAGACCGACCCCGCCGCGCTGGTGGCGCA
GGGGCCGCTGATCCTCGAAAGTGGCGATGGCGTTTTTGTCACTGACAGCAGCGGGCGCAGCTATCTGGAGGGGATGTCCA
GCCTCTGGTGTGCTTCCCTTGGCTTCAATGAGCCGCGTCTGGCCCGCGCGGCGGCTGATCAGATCAGCCGCCTTGGTACT
TACCACACTTTTAACCGGCGCTCGAATCCGGCGGTGATCGAACTGGCGGAGCGGCTTCTGGCGTTGTCTCCGATGCCGGA
TGGCAAGGCGTTGTTCGCCAATTCCGGCTCGGAAGCGGTCGATACGATGATCAAGCTGGCCTGGCTCTACCATAGCGGGC
GCGGTGCGCCAGAAAGGCGCAAGATCATCAGCCGCCGCCGCGCCTATCATGGCAGCACCGTTCTCGGGGCGACGCTCAGC
GGGCTGCCCTCGATGCGTGGTGCCTTTGCCTGGCCGGATCCGGGCGTGATTTTTGCCGAGGCGCCCCATTTCTACACCTG
CGCAGAAGCGGGCGAGAGCGAGGAGGCCTTCGTCGACCGACTGGTGGCCGAAGTCGCGGCTCTCATCGCCGCCGAGGGGG
CTGGCAGCATTGCGGCCATGATCGCAGAACCGGTCATGGGGGCGGGCGGCGTCATAATCCCCCCTTCCGGCTATTTCCCG
AAACTGGCGGAGCTGCTGCGCGAGAAAGGTATCTTGCTGCTCAGCGATGAGGTGATCTGTGGCTTCGGACGCACCGGTGA
ATGGTTCGGCTGCCAGACCTTCGGCTTTCGGCCCGATATGATGAGCGTCGCCAAGGCGCTGTCTTCGGGATATCAGCCGA
TCGGGGCAACCTTGCTGACCGCGGAGATCCACGATGTGGTCAGCGCCGAAGCCAGCCGTCTGGGCGTGCTTGGCCACGGC
TTCACCTATGCCGGGCATCCCGTCACCAGCGCGGTCGCGCTTGAGACGCTGAAGATCTACGAGGAAATGGATCTGCTGGC
ACAGGTTCGGGCGCGGGCACCGGCCTTTCTTGACCATATCAACGCGCTGGCTGGGCATCCGCTTGTGGGCGAGGCCCGCG
CTGTCGGCCTGATCGGTGCAGTGGAACTGGTTGCGGATCGCGCCACGCGCCGACCCTTCGCGTCAGAAGCCGGTATCGGC
GCGCGTCTGGTCACGCTGGCACTGGATCAAGGTCTGATTGTTCGCAATCTGGGCGACGCCATAGCCATTTGCCCGCCGCT
GATCGTCACGACAGCCGAACTGGAGCTACTTTTCAACCGCCTGCGACAGGCGCTGGATGCTCTGGCCCTTGAAACCGCCG
CGAGCCAGGTGGAGGACCGGCATGATCAGTGA

Upstream 100 bases:

>100_bases
CCGCGCCCTTTCCAGCGGCCTCTCTGGTGTTGGTCAAGGCGCTCGGGCGGCCAGAATGGAAACTCGAGATCGAATGTATT
GCGGCCCGGACCGGGGCGGA

Downstream 100 bases:

>100_bases
CATTCTTGATCTCGATGCGACAGCGCAGGCTGCGGCCGTCGCGAAGGGCGATGTCAGCGCGGCAGAGCTGGCCCGCGCCG
CGATTGCGAGGATCGAGGCC

Product: aminotransferase class-III

Products: S-Adenosyl-4-methylthio-2-oxobutanoate; 7,8-Diaminononanoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MMRNSLAHTDLNCLLHPQTDPAALVAQGPLILESGDGVFVTDSSGRSYLEGMSSLWCASLGFNEPRLARAAADQISRLGT
YHTFNRRSNPAVIELAERLLALSPMPDGKALFANSGSEAVDTMIKLAWLYHSGRGAPERRKIISRRRAYHGSTVLGATLS
GLPSMRGAFAWPDPGVIFAEAPHFYTCAEAGESEEAFVDRLVAEVAALIAAEGAGSIAAMIAEPVMGAGGVIIPPSGYFP
KLAELLREKGILLLSDEVICGFGRTGEWFGCQTFGFRPDMMSVAKALSSGYQPIGATLLTAEIHDVVSAEASRLGVLGHG
FTYAGHPVTSAVALETLKIYEEMDLLAQVRARAPAFLDHINALAGHPLVGEARAVGLIGAVELVADRATRRPFASEAGIG
ARLVTLALDQGLIVRNLGDAIAICPPLIVTTAELELLFNRLRQALDALALETAASQVEDRHDQ

Sequences:

>Translated_463_residues
MMRNSLAHTDLNCLLHPQTDPAALVAQGPLILESGDGVFVTDSSGRSYLEGMSSLWCASLGFNEPRLARAAADQISRLGT
YHTFNRRSNPAVIELAERLLALSPMPDGKALFANSGSEAVDTMIKLAWLYHSGRGAPERRKIISRRRAYHGSTVLGATLS
GLPSMRGAFAWPDPGVIFAEAPHFYTCAEAGESEEAFVDRLVAEVAALIAAEGAGSIAAMIAEPVMGAGGVIIPPSGYFP
KLAELLREKGILLLSDEVICGFGRTGEWFGCQTFGFRPDMMSVAKALSSGYQPIGATLLTAEIHDVVSAEASRLGVLGHG
FTYAGHPVTSAVALETLKIYEEMDLLAQVRARAPAFLDHINALAGHPLVGEARAVGLIGAVELVADRATRRPFASEAGIG
ARLVTLALDQGLIVRNLGDAIAICPPLIVTTAELELLFNRLRQALDALALETAASQVEDRHDQ
>Mature_463_residues
MMRNSLAHTDLNCLLHPQTDPAALVAQGPLILESGDGVFVTDSSGRSYLEGMSSLWCASLGFNEPRLARAAADQISRLGT
YHTFNRRSNPAVIELAERLLALSPMPDGKALFANSGSEAVDTMIKLAWLYHSGRGAPERRKIISRRRAYHGSTVLGATLS
GLPSMRGAFAWPDPGVIFAEAPHFYTCAEAGESEEAFVDRLVAEVAALIAAEGAGSIAAMIAEPVMGAGGVIIPPSGYFP
KLAELLREKGILLLSDEVICGFGRTGEWFGCQTFGFRPDMMSVAKALSSGYQPIGATLLTAEIHDVVSAEASRLGVLGHG
FTYAGHPVTSAVALETLKIYEEMDLLAQVRARAPAFLDHINALAGHPLVGEARAVGLIGAVELVADRATRRPFASEAGIG
ARLVTLALDQGLIVRNLGDAIAICPPLIVTTAELELLFNRLRQALDALALETAASQVEDRHDQ

Specific function: Biotin biosynthesis. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI37574042, Length=438, Percent_Identity=29.6803652968037, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI13994255, Length=442, Percent_Identity=29.4117647058824, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI226442705, Length=438, Percent_Identity=28.9954337899543, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI226442709, Length=402, Percent_Identity=30.5970149253731, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI24119277, Length=386, Percent_Identity=29.7927461139896, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI4557809, Length=411, Percent_Identity=27.2506082725061, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI284507298, Length=324, Percent_Identity=27.1604938271605, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1786991, Length=440, Percent_Identity=30.6818181818182, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI145693181, Length=412, Percent_Identity=31.3106796116505, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789016, Length=424, Percent_Identity=29.7169811320755, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788044, Length=425, Percent_Identity=29.6470588235294, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1789759, Length=424, Percent_Identity=28.5377358490566, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787560, Length=418, Percent_Identity=27.511961722488, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1786349, Length=358, Percent_Identity=26.8156424581006, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32564660, Length=436, Percent_Identity=28.2110091743119, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI71992977, Length=440, Percent_Identity=26.8181818181818, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25144271, Length=414, Percent_Identity=26.0869565217391, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25144274, Length=223, Percent_Identity=28.2511210762332, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323470, Length=429, Percent_Identity=25.4079254079254, Blast_Score=136, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6324386, Length=468, Percent_Identity=26.4957264957265, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324432, Length=352, Percent_Identity=23.2954545454545, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6321456, Length=305, Percent_Identity=24.5901639344262, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574759, Length=459, Percent_Identity=28.9760348583878, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI161085790, Length=445, Percent_Identity=28.7640449438202, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21356575, Length=472, Percent_Identity=29.6610169491525, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21357415, Length=422, Percent_Identity=25.5924170616114, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62 [C]

Molecular weight: Translated: 49159; Mature: 49159

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRNSLAHTDLNCLLHPQTDPAALVAQGPLILESGDGVFVTDSSGRSYLEGMSSLWCASL
CCCCCCCCCCCCEEECCCCCCCCEEECCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHC
GFNEPRLARAAADQISRLGTYHTFNRRSNPAVIELAERLLALSPMPDGKALFANSGSEAV
CCCCCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHH
DTMIKLAWLYHSGRGAPERRKIISRRRAYHGSTVLGATLSGLPSMRGAFAWPDPGVIFAE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEC
APHFYTCAEAGESEEAFVDRLVAEVAALIAAEGAGSIAAMIAEPVMGAGGVIIPPSGYFP
CCCEEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCH
KLAELLREKGILLLSDEVICGFGRTGEWFGCQTFGFRPDMMSVAKALSSGYQPIGATLLT
HHHHHHHHCCCEEEECCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
AEIHDVVSAEASRLGVLGHGFTYAGHPVTSAVALETLKIYEEMDLLAQVRARAPAFLDHI
HHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
NALAGHPLVGEARAVGLIGAVELVADRATRRPFASEAGIGARLVTLALDQGLIVRNLGDA
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHCCCHHHHHCCCH
IAICPPLIVTTAELELLFNRLRQALDALALETAASQVEDRHDQ
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMRNSLAHTDLNCLLHPQTDPAALVAQGPLILESGDGVFVTDSSGRSYLEGMSSLWCASL
CCCCCCCCCCCCEEECCCCCCCCEEECCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHC
GFNEPRLARAAADQISRLGTYHTFNRRSNPAVIELAERLLALSPMPDGKALFANSGSEAV
CCCCCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHH
DTMIKLAWLYHSGRGAPERRKIISRRRAYHGSTVLGATLSGLPSMRGAFAWPDPGVIFAE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEC
APHFYTCAEAGESEEAFVDRLVAEVAALIAAEGAGSIAAMIAEPVMGAGGVIIPPSGYFP
CCCEEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCH
KLAELLREKGILLLSDEVICGFGRTGEWFGCQTFGFRPDMMSVAKALSSGYQPIGATLLT
HHHHHHHHCCCEEEECCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
AEIHDVVSAEASRLGVLGHGFTYAGHPVTSAVALETLKIYEEMDLLAQVRARAPAFLDHI
HHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
NALAGHPLVGEARAVGLIGAVELVADRATRRPFASEAGIGARLVTLALDQGLIVRNLGDA
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHCCCHHHHHCCCH
IAICPPLIVTTAELELLFNRLRQALDALALETAASQVEDRHDQ
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Amino-7-oxononanoate; S-Adenosyl-L-methionine [C]

Specific reaction: 8-Amino-7-oxononanoate + S-Adenosyl-L-methionine <==> S-Adenosyl-4-methylthio-2-oxobutanoate + 7,8-Diaminononanoate [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]