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The map label for this gene is prpB [H]

Identifier: 119385710

GI number: 119385710

Start: 159971

End: 160462

Strand: Direct

Name: prpB [H]

Synonym: Pden_2986

Alternate gene names: 119385710

Gene position: 159971-160462 (Clockwise)

Preceding gene: 119385708

Following gene: 119385717

Centisome position: 9.25

GC content: 65.45

Gene sequence:

>492_bases
GTGACCGAGACACTTTATCTGTCGGGCGCGGCCATGACCGCCTCAATGGGCCTGCCCGACCAGGGCATCATCACCGTGGA
CGAGGTCGCGTTCTTCATTCGCCAGATTGCGCAGACCAGTGAGTTGCCGCTGCTGGTCGATGGCGACACGATCTTTCCCG
AGGCGCTGACCTCGGTCGAGAGGTTCCGCGAGGTTCGCGCCCGCCTGCCCGGCGTGAAGCTTCTGGCCAACATGACCGAG
TTCGGCCGCACTCCGGCGCTGACGGCCGAGGAGTTCCAGGAACTGGGCTATGACATGGTGATCTGGCCGGTTTCGTCGCT
GAGGGTCGCGAACAAGGCGCAGGAGCGGTTTTACGCGGCGCTGGCCCGCGACGGCGCGACGACGGCCATGCTGCCCGAGA
TGCAGACCCGCGCCCAGCTTTACGAGTTGATCGGCCTCAACGCCTTCGAGGCGCTGGACGCCTCGATCGCGCAAAGCGTC
CTGCCCGCATAA

Upstream 100 bases:

>100_bases
GGTGCCGCCGAAGCGCGAGAACGGGAACCGCCCGGCGCTGCCTCGTTGACCGTCTGCTTGGCGCGCATAACGGCATAGTC
GCATCGAAGCCTGCGCCGTC

Downstream 100 bases:

>100_bases
GGCCAGGGATCGGGCGGGGGGGTCCCGCCCGACGACCGGCGGGGCGCAACCGCCCACCGCTCCGCCGGTTCATGCGCGAG
GGTTTCGCGGCGGCAGGCCG

Product: PEP phosphonomutase and related enzymes-like

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MTETLYLSGAAMTASMGLPDQGIITVDEVAFFIRQIAQTSELPLLVDGDTIFPEALTSVERFREVRARLPGVKLLANMTE
FGRTPALTAEEFQELGYDMVIWPVSSLRVANKAQERFYAALARDGATTAMLPEMQTRAQLYELIGLNAFEALDASIAQSV
LPA

Sequences:

>Translated_163_residues
MTETLYLSGAAMTASMGLPDQGIITVDEVAFFIRQIAQTSELPLLVDGDTIFPEALTSVERFREVRARLPGVKLLANMTE
FGRTPALTAEEFQELGYDMVIWPVSSLRVANKAQERFYAALARDGATTAMLPEMQTRAQLYELIGLNAFEALDASIAQSV
LPA
>Mature_162_residues
TETLYLSGAAMTASMGLPDQGIITVDEVAFFIRQIAQTSELPLLVDGDTIFPEALTSVERFREVRARLPGVKLLANMTEF
GRTPALTAEEFQELGYDMVIWPVSSLRVANKAQERFYAALARDGATTAMLPEMQTRAQLYELIGLNAFEALDASIAQSVL
PA

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=110, Percent_Identity=38.1818181818182, Blast_Score=75, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 17848; Mature: 17717

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETLYLSGAAMTASMGLPDQGIITVDEVAFFIRQIAQTSELPLLVDGDTIFPEALTSVE
CCCEEEECCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHH
RFREVRARLPGVKLLANMTEFGRTPALTAEEFQELGYDMVIWPVSSLRVANKAQERFYAA
HHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHH
LARDGATTAMLPEMQTRAQLYELIGLNAFEALDASIAQSVLPA
HHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TETLYLSGAAMTASMGLPDQGIITVDEVAFFIRQIAQTSELPLLVDGDTIFPEALTSVE
CCEEEECCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHH
RFREVRARLPGVKLLANMTEFGRTPALTAEEFQELGYDMVIWPVSSLRVANKAQERFYAA
HHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHH
LARDGATTAMLPEMQTRAQLYELIGLNAFEALDASIAQSVLPA
HHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]