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The map label for this gene is mdtE [H]

Identifier: 119385666

GI number: 119385666

Start: 105290

End: 106483

Strand: Direct

Name: mdtE [H]

Synonym: Pden_2941

Alternate gene names: 119385666

Gene position: 105290-106483 (Clockwise)

Preceding gene: 119385665

Following gene: 119385667

Centisome position: 6.09

GC content: 72.45

Gene sequence:

>1194_bases
ATGACCATGAGACTTTCTCCCGGCGTCTGCGCCGGGCTTGTGCTTTGCTGCCTGATGGCGGCCGCCCCGGCGGCGGCGCA
GCAATTGCCGCCGCCGCAGGTCAGCGTGCAGGAAATGACGCCCGAGGCGCTGCCGGTGGTGAACGAATTGCCCGGCCGCG
TGGCCGCGACCCGCACCGCCGAGGTGCGGCCGCGCGTCGGCGGCATCGTGATCGAGCGCGTCTTCGAGCAGGGCGGCAAG
GTCGAGGCGGGCGATGTGCTTTACCGCATCGACCCCGCCCCCTATGACGTGCGCGTCGCCAGCGCCGAGGGCGCGCTGGC
CCGGGCCGAGGCCACGGCGCTGAACGCCGCCGACCAGGAAAAGCGTGCCCGCGCCCTGCGCGAGCGCAAGGTGACGGCGG
GGGTCGACCTGGACAATGCCGCCACCGCGCTGGCCCAGGCCCAGGCGGATGTGGCCATCGCCCGCGCCTCGCTGCAGGAG
GCGCAGCTGAACCGTTCCTATACCGAGGTCGCGGCGCCCATCGCCGGCGTGGTCGGCCGGGCGCTGGTGACCGAGGGCGC
GCTGGTCAACGCCCAGACCGACATCATGGCGACGATCCAGCAGCTCGACCCGGTCTATGTGGACTTCACCCAGTCCTCGT
CCGAACTGTTCGCGTTGCGCCGGGCCCTGGCGGCGGGGCAACTGGCCATGATCTCGGCCGACGCGGCGGCGGTGCGGCTG
ATCTTCGACGACGGCAGCGAATATGCCCATCCCGGCAAGCTGCTGTTTTCCGAGGCGAGCGTGGACAGCTCGACCGGGCA
GATCACGCTGCGCGCCGAGTTCCCGAACCCCGAGGGCGACCTGCTGCCCGGGCTTTACGTCCGGGTGCGGATCGAGCAGG
CGGTGCGCGACAACGCCCTGACCGTGCCGCAGATGGCGGTGCAGCGCGACCAGTCGGGCCAGGCCTATGTCTATGCGCTG
AAGGATGCCGACACGGTCGAGCGCCGCGACGTGACGCTGGGCCAGACCGCCGGCAACCGCTGGCTGGTGGAAAGCGGGCT
GAAGCCGGGCGACCGGGTCGTGGTCGCCGGAGCGCAGAAGCTTTACCCCGACGCCAAGGTGGTGCCGCAGCCTGTGGACG
CGGCCCAGCCGGCGGTGCCGGCCGGTGCCGCCGCGGCGGCCGAAGCGCCCGCCCCGGCGCCTGCGCAGGAGTAG

Upstream 100 bases:

>100_bases
ACGAGATCCTTGATGAATTGATGGCGGTCGCGGAAAGCGACCCCGGCGCAGCCGGAGACTGACCCCTCCCCGAACCATTC
AGCCTTCCCAGGAAAGAACC

Downstream 100 bases:

>100_bases
GGCGATGGCACGGTTCTTCATTCACCGGCCGGTGCTGGCCTGGGTGATCTCGATCTTCATCGTCATCGCCGGGGTGCTGG
CGCTGCCGATGATGCCCATC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTMRLSPGVCAGLVLCCLMAAAPAAAQQLPPPQVSVQEMTPEALPVVNELPGRVAATRTAEVRPRVGGIVIERVFEQGGK
VEAGDVLYRIDPAPYDVRVASAEGALARAEATALNAADQEKRARALRERKVTAGVDLDNAATALAQAQADVAIARASLQE
AQLNRSYTEVAAPIAGVVGRALVTEGALVNAQTDIMATIQQLDPVYVDFTQSSSELFALRRALAAGQLAMISADAAAVRL
IFDDGSEYAHPGKLLFSEASVDSSTGQITLRAEFPNPEGDLLPGLYVRVRIEQAVRDNALTVPQMAVQRDQSGQAYVYAL
KDADTVERRDVTLGQTAGNRWLVESGLKPGDRVVVAGAQKLYPDAKVVPQPVDAAQPAVPAGAAAAAEAPAPAPAQE

Sequences:

>Translated_397_residues
MTMRLSPGVCAGLVLCCLMAAAPAAAQQLPPPQVSVQEMTPEALPVVNELPGRVAATRTAEVRPRVGGIVIERVFEQGGK
VEAGDVLYRIDPAPYDVRVASAEGALARAEATALNAADQEKRARALRERKVTAGVDLDNAATALAQAQADVAIARASLQE
AQLNRSYTEVAAPIAGVVGRALVTEGALVNAQTDIMATIQQLDPVYVDFTQSSSELFALRRALAAGQLAMISADAAAVRL
IFDDGSEYAHPGKLLFSEASVDSSTGQITLRAEFPNPEGDLLPGLYVRVRIEQAVRDNALTVPQMAVQRDQSGQAYVYAL
KDADTVERRDVTLGQTAGNRWLVESGLKPGDRVVVAGAQKLYPDAKVVPQPVDAAQPAVPAGAAAAAEAPAPAPAQE
>Mature_396_residues
TMRLSPGVCAGLVLCCLMAAAPAAAQQLPPPQVSVQEMTPEALPVVNELPGRVAATRTAEVRPRVGGIVIERVFEQGGKV
EAGDVLYRIDPAPYDVRVASAEGALARAEATALNAADQEKRARALRERKVTAGVDLDNAATALAQAQADVAIARASLQEA
QLNRSYTEVAAPIAGVVGRALVTEGALVNAQTDIMATIQQLDPVYVDFTQSSSELFALRRALAAGQLAMISADAAAVRLI
FDDGSEYAHPGKLLFSEASVDSSTGQITLRAEFPNPEGDLLPGLYVRVRIEQAVRDNALTVPQMAVQRDQSGQAYVYALK
DADTVERRDVTLGQTAGNRWLVESGLKPGDRVVVAGAQKLYPDAKVVPQPVDAAQPAVPAGAAAAAEAPAPAPAQE

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=358, Percent_Identity=41.340782122905, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1789665, Length=360, Percent_Identity=43.8888888888889, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI1786668, Length=359, Percent_Identity=44.2896935933148, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI87082036, Length=339, Percent_Identity=29.4985250737463, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI87081796, Length=325, Percent_Identity=27.6923076923077, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87081951, Length=127, Percent_Identity=31.496062992126, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41570; Mature: 41438

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMRLSPGVCAGLVLCCLMAAAPAAAQQLPPPQVSVQEMTPEALPVVNELPGRVAATRTA
CCEECCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHCCCCHHHHHHHCCCCEEECCCC
EVRPRVGGIVIERVFEQGGKVEAGDVLYRIDPAPYDVRVASAEGALARAEATALNAADQE
CCCCCHHHHHHHHHHHCCCCEECCCEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCHHH
KRARALRERKVTAGVDLDNAATALAQAQADVAIARASLQEAQLNRSYTEVAAPIAGVVGR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ALVTEGALVNAQTDIMATIQQLDPVYVDFTQSSSELFALRRALAAGQLAMISADAAAVRL
HHHHCCCEECCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCEEEEE
IFDDGSEYAHPGKLLFSEASVDSSTGQITLRAEFPNPEGDLLPGLYVRVRIEQAVRDNAL
EEECCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEHHHHCCCCC
TVPQMAVQRDQSGQAYVYALKDADTVERRDVTLGQTAGNRWLVESGLKPGDRVVVAGAQK
CCHHHHHHCCCCCCEEEEEECCCCCHHHCCCEECCCCCCEEEEECCCCCCCEEEEECHHH
LYPDAKVVPQPVDAAQPAVPAGAAAAAEAPAPAPAQE
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TMRLSPGVCAGLVLCCLMAAAPAAAQQLPPPQVSVQEMTPEALPVVNELPGRVAATRTA
CEECCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHCCCCHHHHHHHCCCCEEECCCC
EVRPRVGGIVIERVFEQGGKVEAGDVLYRIDPAPYDVRVASAEGALARAEATALNAADQE
CCCCCHHHHHHHHHHHCCCCEECCCEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCHHH
KRARALRERKVTAGVDLDNAATALAQAQADVAIARASLQEAQLNRSYTEVAAPIAGVVGR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ALVTEGALVNAQTDIMATIQQLDPVYVDFTQSSSELFALRRALAAGQLAMISADAAAVRL
HHHHCCCEECCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCEEEEE
IFDDGSEYAHPGKLLFSEASVDSSTGQITLRAEFPNPEGDLLPGLYVRVRIEQAVRDNAL
EEECCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEHHHHCCCCC
TVPQMAVQRDQSGQAYVYALKDADTVERRDVTLGQTAGNRWLVESGLKPGDRVVVAGAQK
CCHHHHHHCCCCCCEEEEEECCCCCHHHCCCEECCCCCCEEEEECCCCCCCEEEEECHHH
LYPDAKVVPQPVDAAQPAVPAGAAAAAEAPAPAPAQE
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]