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The map label for this gene is bioB [H]

Identifier: 119385644

GI number: 119385644

Start: 81948

End: 83231

Strand: Direct

Name: bioB [H]

Synonym: Pden_2919

Alternate gene names: 119385644

Gene position: 81948-83231 (Clockwise)

Preceding gene: 119385643

Following gene: 119385645

Centisome position: 4.74

GC content: 71.18

Gene sequence:

>1284_bases
ATGCGGGATCTGGCGTTCGACCGGCGGCATCTGTGGCATCCCTACAGCTCGATGCGCGAGCCGGGGCCGGTGCATGAGGT
CGCGGCGGCCGAGGGGGTCTGGCTGGAACTGGCGGACGGGGTGCGGATGATCGACGCCATGTCCTCGTGGTGGGCGGCGG
CGCATGGGCATCGCCATCCGCGGCTGGTCGAGGCGATGCAGGCGCAGCTGGACCGCCTGCCGCATGTGATGTTCGGCGGG
CTGACGCACGCCCCGGCCGTGGGGCTGGCGCGGCGACTGGTCGCGATGCTGCCCGTGGGGCTGGACCGCATCTTTTACAG
CGACAGCGGCTCGGTCGCGGTCGAGGTGGCGCTGAAGATGGCGGTGCAGGCGCAGCTTGGGCTGGGGCAGGCGGGGCGGG
TGGCTTTCGCCTCGGCCCGCGGCGGCTATTACGGCGATACCTGGAAGGCGATGAGCCTGTGCGATCCGCAGACCGGGATG
CATGCCCATTTCGGCGCCGCCTTGCAGGTCCAGCATTTCGTGCCGCGCCCGCCCATTCCTTTCGGCGCGGAATGGGACGA
GGATCCGGCGCGGAACGGGCTGGGCGCGGTCGAGGCGCTGTTTGCCGGGAAAGCGCATGAAATCGCGGGTTTCATCGTCG
AGCCGGTGGTGCAGGGCGCAGGGGGCATGTGGTTCTATCACCCGCGCTGGCTGGCCGGGCTGCGCACGCTGTGCGACCGG
CATGGCGTGTTGCTGATTTTCGACGAGATCGCCACCGGATTCGGCCGAACCGGCACGCTGTTCGCCATGGAGCGGGCAGG
GGTGGTGCCGGATATCCTGTGCCTGGGCAAGGCGCTGACCGGGGGCATGATGAGCTTTGCCGCCACCGTCGCATCGGGCC
GGGTGGCCGAGGCGATCTCGGACGGGCCGGCGCCGGTGCTGATGCACGGGCCGACCTTCATGGGCAATCCGCTGGCCTGT
GCGGTGGCTTGCGCGAGCCTGGACCTGCTGGCGGAAGGCGCATGGCAGGCGCAGGTCGCGGGGATCGAGGCGGGGTTGGC
GCAGGGCCTTGCCCCGGCGCGCGGCCTGCCCGGCGTGCGCGACGTGCGGGTGCTGGGCGCCATCGGGGTGATCGAGACGC
AGGCGCCGGTGGACATGGGCCGCGTGCATGGCTTTTGCCGCGACAGCGGGGTCTGGCTGCGGCCGTTCGGGCGGCTGCTT
TACTGCATGCCGCCCTTTGTCGCCGCGCCGGACGAGGTCGCGCGCATCTGCGCCGCGATGGTCGAGATCGCGGGCTGGCC
ATGA

Upstream 100 bases:

>100_bases
GCGACCTGGGCCGGCTGCCGGTGCTGGAGCGGGTGACGGCCGAGGCGCTGGCCGATGCCTTTGCCGGCATCGACCTGGCG
GCGATCCGCGAGGTGTCGTG

Downstream 100 bases:

>100_bases
GGGCGGAATGGCTGCGCCGGGAAGGAGCGCCGGAGCTGATCGTGGTGCTGACCGGCTGGGCGGTCGGGGCGGGGCCGTTC
CGGCATCTGGCCGGGCCGGC

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MRDLAFDRRHLWHPYSSMREPGPVHEVAAAEGVWLELADGVRMIDAMSSWWAAAHGHRHPRLVEAMQAQLDRLPHVMFGG
LTHAPAVGLARRLVAMLPVGLDRIFYSDSGSVAVEVALKMAVQAQLGLGQAGRVAFASARGGYYGDTWKAMSLCDPQTGM
HAHFGAALQVQHFVPRPPIPFGAEWDEDPARNGLGAVEALFAGKAHEIAGFIVEPVVQGAGGMWFYHPRWLAGLRTLCDR
HGVLLIFDEIATGFGRTGTLFAMERAGVVPDILCLGKALTGGMMSFAATVASGRVAEAISDGPAPVLMHGPTFMGNPLAC
AVACASLDLLAEGAWQAQVAGIEAGLAQGLAPARGLPGVRDVRVLGAIGVIETQAPVDMGRVHGFCRDSGVWLRPFGRLL
YCMPPFVAAPDEVARICAAMVEIAGWP

Sequences:

>Translated_427_residues
MRDLAFDRRHLWHPYSSMREPGPVHEVAAAEGVWLELADGVRMIDAMSSWWAAAHGHRHPRLVEAMQAQLDRLPHVMFGG
LTHAPAVGLARRLVAMLPVGLDRIFYSDSGSVAVEVALKMAVQAQLGLGQAGRVAFASARGGYYGDTWKAMSLCDPQTGM
HAHFGAALQVQHFVPRPPIPFGAEWDEDPARNGLGAVEALFAGKAHEIAGFIVEPVVQGAGGMWFYHPRWLAGLRTLCDR
HGVLLIFDEIATGFGRTGTLFAMERAGVVPDILCLGKALTGGMMSFAATVASGRVAEAISDGPAPVLMHGPTFMGNPLAC
AVACASLDLLAEGAWQAQVAGIEAGLAQGLAPARGLPGVRDVRVLGAIGVIETQAPVDMGRVHGFCRDSGVWLRPFGRLL
YCMPPFVAAPDEVARICAAMVEIAGWP
>Mature_427_residues
MRDLAFDRRHLWHPYSSMREPGPVHEVAAAEGVWLELADGVRMIDAMSSWWAAAHGHRHPRLVEAMQAQLDRLPHVMFGG
LTHAPAVGLARRLVAMLPVGLDRIFYSDSGSVAVEVALKMAVQAQLGLGQAGRVAFASARGGYYGDTWKAMSLCDPQTGM
HAHFGAALQVQHFVPRPPIPFGAEWDEDPARNGLGAVEALFAGKAHEIAGFIVEPVVQGAGGMWFYHPRWLAGLRTLCDR
HGVLLIFDEIATGFGRTGTLFAMERAGVVPDILCLGKALTGGMMSFAATVASGRVAEAISDGPAPVLMHGPTFMGNPLAC
AVACASLDLLAEGAWQAQVAGIEAGLAQGLAPARGLPGVRDVRVLGAIGVIETQAPVDMGRVHGFCRDSGVWLRPFGRLL
YCMPPFVAAPDEVARICAAMVEIAGWP

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=422, Percent_Identity=30.0947867298578, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI284507298, Length=310, Percent_Identity=31.9354838709677, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI13994255, Length=390, Percent_Identity=28.2051282051282, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI24119277, Length=418, Percent_Identity=25.8373205741627, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1786991, Length=418, Percent_Identity=56.9377990430622, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI145693181, Length=305, Percent_Identity=35.4098360655738, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789016, Length=390, Percent_Identity=30.5128205128205, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787560, Length=353, Percent_Identity=33.4277620396601, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI1788044, Length=303, Percent_Identity=35.6435643564356, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1789759, Length=295, Percent_Identity=33.8983050847458, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786349, Length=387, Percent_Identity=31.5245478036176, Blast_Score=112, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI25144271, Length=419, Percent_Identity=29.1169451073986, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25144274, Length=227, Percent_Identity=34.3612334801762, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6324386, Length=456, Percent_Identity=40.1315789473684, Blast_Score=338, Evalue=8e-94,
Organism=Saccharomyces cerevisiae, GI6323470, Length=420, Percent_Identity=27.3809523809524, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324432, Length=311, Percent_Identity=30.5466237942122, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21357415, Length=416, Percent_Identity=30.2884615384615, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI161085790, Length=458, Percent_Identity=26.6375545851528, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28574759, Length=440, Percent_Identity=26.5909090909091, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21356575, Length=323, Percent_Identity=25.6965944272446, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR007197 [H]

Pfam domain/function: PF00202 Aminotran_3; PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 45427; Mature: 45427

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00600 AA_TRANSFER_CLASS_3 ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLAFDRRHLWHPYSSMREPGPVHEVAAAEGVWLELADGVRMIDAMSSWWAAAHGHRHP
CCCCCCCHHHHCCCHHHHCCCCCHHHHHHCCCCEEEHHCCHHHHHHHHHHHHHHCCCCCH
RLVEAMQAQLDRLPHVMFGGLTHAPAVGLARRLVAMLPVGLDRIFYSDSGSVAVEVALKM
HHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHEEECCCCCEEHHHHHHH
AVQAQLGLGQAGRVAFASARGGYYGDTWKAMSLCDPQTGMHAHFGAALQVQHFVPRPPIP
HHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCHHCCCCEEEEECCCCCCCC
FGAEWDEDPARNGLGAVEALFAGKAHEIAGFIVEPVVQGAGGMWFYHPRWLAGLRTLCDR
CCCCCCCCHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHC
HGVLLIFDEIATGFGRTGTLFAMERAGVVPDILCLGKALTGGMMSFAATVASGRVAEAIS
CCEEEEEHHHHCCCCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHC
DGPAPVLMHGPTFMGNPLACAVACASLDLLAEGAWQAQVAGIEAGLAQGLAPARGLPGVR
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCH
DVRVLGAIGVIETQAPVDMGRVHGFCRDSGVWLRPFGRLLYCMPPFVAAPDEVARICAAM
HHHHHHHHCEEECCCCCCHHHHCCHHCCCCEEECHHHHHHHHCCCCCCCCHHHHHHHHHH
VEIAGWP
HHHCCCC
>Mature Secondary Structure
MRDLAFDRRHLWHPYSSMREPGPVHEVAAAEGVWLELADGVRMIDAMSSWWAAAHGHRHP
CCCCCCCHHHHCCCHHHHCCCCCHHHHHHCCCCEEEHHCCHHHHHHHHHHHHHHCCCCCH
RLVEAMQAQLDRLPHVMFGGLTHAPAVGLARRLVAMLPVGLDRIFYSDSGSVAVEVALKM
HHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHEEECCCCCEEHHHHHHH
AVQAQLGLGQAGRVAFASARGGYYGDTWKAMSLCDPQTGMHAHFGAALQVQHFVPRPPIP
HHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCHHCCCCEEEEECCCCCCCC
FGAEWDEDPARNGLGAVEALFAGKAHEIAGFIVEPVVQGAGGMWFYHPRWLAGLRTLCDR
CCCCCCCCHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHC
HGVLLIFDEIATGFGRTGTLFAMERAGVVPDILCLGKALTGGMMSFAATVASGRVAEAIS
CCEEEEEHHHHCCCCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHC
DGPAPVLMHGPTFMGNPLACAVACASLDLLAEGAWQAQVAGIEAGLAQGLAPARGLPGVR
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCH
DVRVLGAIGVIETQAPVDMGRVHGFCRDSGVWLRPFGRLLYCMPPFVAAPDEVARICAAM
HHHHHHHHCEEECCCCCCHHHHCCHHCCCCEEECHHHHHHHHCCCCCCCCHHHHHHHHHH
VEIAGWP
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA