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The map label for this gene is dnaN [H]

Identifier: 119385559

GI number: 119385559

Start: 1843

End: 2961

Strand: Direct

Name: dnaN [H]

Synonym: Pden_2834

Alternate gene names: 119385559

Gene position: 1843-2961 (Clockwise)

Preceding gene: 119385558

Following gene: 119385560

Centisome position: 0.11

GC content: 67.47

Gene sequence:

>1119_bases
ATGAAATTCTCGATCGAACGTGCCGATCTGGTGAAAGCCGTATCCCAGGCCCAGTCCGTGGTCGAACGCCGCAACACCAT
CCCGATCCTTGCCAACGTGCTGATCGAGGCCGCGCCCGAGGGCGTCAGCTTCCGCGCCACCGACCTGGATACCGAGATCG
TGGACCGCGCGCCGGCGCAGGTCGAGCGGCCCGGCGCCACCACCGTCTCGGCCGGTATGCTGAACGACATCGCCCGCAAG
CTGCCGGACGGCGCGCTGGTGCAGCTTAGCCTGGATGCCGCCTCGGGGCGGCTTTCCGTGCAGGCCGGGCGCTCGAACTT
CAGCCTCGCCACGCTGCCGCGCGAGGATTTCCCGGTCATGTCCTCGTCGGAATACGGCGCGAATTTCTCGGCCCCCGCCG
CGGTGCTGCGGCGGCTGTTCGACAAGTCGAAATTCGCCATCTCGAACGAAGAAACCCGCTATTATCTCAACGGCGTCTAT
ATGCATGTCGCGCAGACCGAGGACGGCCCCGGCCTGCGCTGCGTGGCGACCGACGGCCACCGGCTGGCCCGAATCGACGC
CCCGCTGCCGGCGGGCGCCGAGGACATGCCGGGGGTGATCGTGCCGCGCAAGACCGTGGCCGAGCTGCGCAAGCTGCTGG
ACGATGACGAGGCCGAGATCGCGGTCTCGGTCAGCGAGACCAAGGTGCGCTTCGCCACGCCGACGATCACGCTGACCTCG
AAGGTGATCGACGGCACCTTCCCCGATTACAGCCGGGTCATCCCCTCGGGCAATGCCCGCAAGCTGGAGGTCGATGCCGG
CGATTTCGCCAAGGCGGTCGACCGGGTGGCCACCGTCAGCAGCGAACGCTCGCGCGCCGTCAAGCTGTCGCTGGACGAGG
ATCGGCTGGTCCTGTCGGTCAATGCCCCCGATGCCGGCGCCGCTGATGAGGAACTGCCGGTCGCCTATGCCGACGAGCCG
CTGGAGATCGGCTTCAACGCCAAATACCTGCACGAGATCGCCAGCCAGATCGAGCGCGAGAACGCTGTCTTCCTGTTCAA
CGGCTCGGGCGACGCGGCGCTGATCCGCGAGGGCGGCGACACCAGCGCGGTCTATGTCGTCATGCCGATGCGCGTGTGA

Upstream 100 bases:

>100_bases
GAGGCCTGAACGCATCCGCTTGACCTTGCGCGGGATTATGCCACATTGGCGCCCCGCGAGGCGGGTGCGAGACGAGGCCG
GAACGACATAAGGGTTGACC

Downstream 100 bases:

>100_bases
CGCTGAGCCTGCTGCACCTGACCCAGTTTCGCAGCTGGGCCAGGCTGGAGATCGAGGCCGACCACCGCCCCGTCGCCATC
CATGGCCCGAACGGGGCGGG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKFSIERADLVKAVSQAQSVVERRNTIPILANVLIEAAPEGVSFRATDLDTEIVDRAPAQVERPGATTVSAGMLNDIARK
LPDGALVQLSLDAASGRLSVQAGRSNFSLATLPREDFPVMSSSEYGANFSAPAAVLRRLFDKSKFAISNEETRYYLNGVY
MHVAQTEDGPGLRCVATDGHRLARIDAPLPAGAEDMPGVIVPRKTVAELRKLLDDDEAEIAVSVSETKVRFATPTITLTS
KVIDGTFPDYSRVIPSGNARKLEVDAGDFAKAVDRVATVSSERSRAVKLSLDEDRLVLSVNAPDAGAADEELPVAYADEP
LEIGFNAKYLHEIASQIERENAVFLFNGSGDAALIREGGDTSAVYVVMPMRV

Sequences:

>Translated_372_residues
MKFSIERADLVKAVSQAQSVVERRNTIPILANVLIEAAPEGVSFRATDLDTEIVDRAPAQVERPGATTVSAGMLNDIARK
LPDGALVQLSLDAASGRLSVQAGRSNFSLATLPREDFPVMSSSEYGANFSAPAAVLRRLFDKSKFAISNEETRYYLNGVY
MHVAQTEDGPGLRCVATDGHRLARIDAPLPAGAEDMPGVIVPRKTVAELRKLLDDDEAEIAVSVSETKVRFATPTITLTS
KVIDGTFPDYSRVIPSGNARKLEVDAGDFAKAVDRVATVSSERSRAVKLSLDEDRLVLSVNAPDAGAADEELPVAYADEP
LEIGFNAKYLHEIASQIERENAVFLFNGSGDAALIREGGDTSAVYVVMPMRV
>Mature_372_residues
MKFSIERADLVKAVSQAQSVVERRNTIPILANVLIEAAPEGVSFRATDLDTEIVDRAPAQVERPGATTVSAGMLNDIARK
LPDGALVQLSLDAASGRLSVQAGRSNFSLATLPREDFPVMSSSEYGANFSAPAAVLRRLFDKSKFAISNEETRYYLNGVY
MHVAQTEDGPGLRCVATDGHRLARIDAPLPAGAEDMPGVIVPRKTVAELRKLLDDDEAEIAVSVSETKVRFATPTITLTS
KVIDGTFPDYSRVIPSGNARKLEVDAGDFAKAVDRVATVSSERSRAVKLSLDEDRLVLSVNAPDAGAADEELPVAYADEP
LEIGFNAKYLHEIASQIERENAVFLFNGSGDAALIREGGDTSAVYVVMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=38.1333333333333, Blast_Score=258, Evalue=4e-70,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39994; Mature: 39994

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSIERADLVKAVSQAQSVVERRNTIPILANVLIEAAPEGVSFRATDLDTEIVDRAPAQ
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHCCCCC
VERPGATTVSAGMLNDIARKLPDGALVQLSLDAASGRLSVQAGRSNFSLATLPREDFPVM
CCCCCCCEEHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCEEEECCCCCCCCC
SSSEYGANFSAPAAVLRRLFDKSKFAISNEETRYYLNGVYMHVAQTEDGPGLRCVATDGH
CCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCEEEEEEEEEEEEECCCCCCEEEEECCCC
RLARIDAPLPAGAEDMPGVIVPRKTVAELRKLLDDDEAEIAVSVSETKVRFATPTITLTS
EEEEECCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCEEEEEEECCEEEEECCEEEEEH
KVIDGTFPDYSRVIPSGNARKLEVDAGDFAKAVDRVATVSSERSRAVKLSLDEDRLVLSV
HHHCCCCCCHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEE
NAPDAGAADEELPVAYADEPLEIGFNAKYLHEIASQIERENAVFLFNGSGDAALIREGGD
CCCCCCCCCCCCCEEECCCCEEECCCHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCC
TSAVYVVMPMRV
CEEEEEEEECCC
>Mature Secondary Structure
MKFSIERADLVKAVSQAQSVVERRNTIPILANVLIEAAPEGVSFRATDLDTEIVDRAPAQ
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHCCCCC
VERPGATTVSAGMLNDIARKLPDGALVQLSLDAASGRLSVQAGRSNFSLATLPREDFPVM
CCCCCCCEEHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCEEEECCCCCCCCC
SSSEYGANFSAPAAVLRRLFDKSKFAISNEETRYYLNGVYMHVAQTEDGPGLRCVATDGH
CCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCEEEEEEEEEEEEECCCCCCEEEEECCCC
RLARIDAPLPAGAEDMPGVIVPRKTVAELRKLLDDDEAEIAVSVSETKVRFATPTITLTS
EEEEECCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCEEEEEEECCEEEEECCEEEEEH
KVIDGTFPDYSRVIPSGNARKLEVDAGDFAKAVDRVATVSSERSRAVKLSLDEDRLVLSV
HHHCCCCCCHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEE
NAPDAGAADEELPVAYADEPLEIGFNAKYLHEIASQIERENAVFLFNGSGDAALIREGGD
CCCCCCCCCCCCCEEECCCCEEECCCHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCC
TSAVYVVMPMRV
CEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]