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The map label for this gene is recF [H]

Identifier: 119385560

GI number: 119385560

Start: 2958

End: 4034

Strand: Direct

Name: recF [H]

Synonym: Pden_2835

Alternate gene names: 119385560

Gene position: 2958-4034 (Clockwise)

Preceding gene: 119385559

Following gene: 119385562

Centisome position: 0.17

GC content: 72.14

Gene sequence:

>1077_bases
GTGACGCTGAGCCTGCTGCACCTGACCCAGTTTCGCAGCTGGGCCAGGCTGGAGATCGAGGCCGACCACCGCCCCGTCGC
CATCCATGGCCCGAACGGGGCGGGCAAGACCAATATCCTGGAAGCCGTCTCGATGCTGTCGCCCGGCCGGGGCATGCGCG
GCGCCGCACCCGGCGACCAGGCCCGCAAGGGGCCGGAGGTCGGCTGGCAGATCCGGGCCGAAATCGGCACCCATCAGGTG
CTGACCCGCGCGCTGCCCGGCCAGCCGCGCGAGGTGGTGATCGACGAAAAGCCCTCGACCCAGATCGCGCTTGGCCGGCT
GATGCGGGTGATCTGGCTGACGCCCGCCATGGACCGGCTGTGGACCGATGCCCCGGAACAGCGGCGGCGATTCCTGGACC
GGGTGACGCTCAGCTTCACGCCGGGCCATGCCGAGGACGCGCTGGGTTACGAAAAGGCCATGCGCGAACGCAACCGGCTG
CTGCGCGACGAGGTCCGCGACGCCGGCTGGTATCGCGCGCTGGAGGCACAGATGGCCGAGACGGGCGCGGCGCTGACGCG
CAACCGCCTGGACGCCATCGCCCGGATCATGGCGGCGCAGGAGGGGGCCGGGACCGCCTTCCCCTCGGCCAGCCTGACGC
TGTTGCCGGGCGAAGGTTCGGCCGACGATCCCGACGCCGAGAGCATCGCCGCCCGGCTGGCCGAGGGCCGCGGCCGCGAC
ATGGCGGCCGGCCGCACCCTGACCGGCCCGCATCGCGCCGACCTGGGGGCGCATTGGGGGCCGCAGGCGATGCCCGCCGC
CCTTTCCTCAACCGGCGAGCAAAAGGCGCTGCTCTTGTCGCTGATCCTCGCCAATGCCCGGGCGCTGGCCGAGGAAAGCC
CGGTGCTGCTGCTCGACGAGGTGGCGGCGCATCTGGATGCCGACCGCCGCGCAGCGCTTTACGACGAGATCTGCGCGCTT
CGCGCCCAGGCCTGGCTGACCGGTACCGGGCCGGAACTGTTCGAGGCGCTACGCGGCCGGGCGCAATTTCTGGCGGTGGT
GCGCGAGGCCGAGGCCTCGCGCATCATGGGTATTTGA

Upstream 100 bases:

>100_bases
GCGAGAACGCTGTCTTCCTGTTCAACGGCTCGGGCGACGCGGCGCTGATCCGCGAGGGCGGCGACACCAGCGCGGTCTAT
GTCGTCATGCCGATGCGCGT

Downstream 100 bases:

>100_bases
GAAACGGAAAGACGCCTGCCGGTCGCGCGAAGGCTAGACCATCAGGTCCACCAGTTCGAAGCGGGTCACGTCGACGATGC
CGAGGTCCGAGATCCGCAGC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MTLSLLHLTQFRSWARLEIEADHRPVAIHGPNGAGKTNILEAVSMLSPGRGMRGAAPGDQARKGPEVGWQIRAEIGTHQV
LTRALPGQPREVVIDEKPSTQIALGRLMRVIWLTPAMDRLWTDAPEQRRRFLDRVTLSFTPGHAEDALGYEKAMRERNRL
LRDEVRDAGWYRALEAQMAETGAALTRNRLDAIARIMAAQEGAGTAFPSASLTLLPGEGSADDPDAESIAARLAEGRGRD
MAAGRTLTGPHRADLGAHWGPQAMPAALSSTGEQKALLLSLILANARALAEESPVLLLDEVAAHLDADRRAALYDEICAL
RAQAWLTGTGPELFEALRGRAQFLAVVREAEASRIMGI

Sequences:

>Translated_358_residues
MTLSLLHLTQFRSWARLEIEADHRPVAIHGPNGAGKTNILEAVSMLSPGRGMRGAAPGDQARKGPEVGWQIRAEIGTHQV
LTRALPGQPREVVIDEKPSTQIALGRLMRVIWLTPAMDRLWTDAPEQRRRFLDRVTLSFTPGHAEDALGYEKAMRERNRL
LRDEVRDAGWYRALEAQMAETGAALTRNRLDAIARIMAAQEGAGTAFPSASLTLLPGEGSADDPDAESIAARLAEGRGRD
MAAGRTLTGPHRADLGAHWGPQAMPAALSSTGEQKALLLSLILANARALAEESPVLLLDEVAAHLDADRRAALYDEICAL
RAQAWLTGTGPELFEALRGRAQFLAVVREAEASRIMGI
>Mature_357_residues
TLSLLHLTQFRSWARLEIEADHRPVAIHGPNGAGKTNILEAVSMLSPGRGMRGAAPGDQARKGPEVGWQIRAEIGTHQVL
TRALPGQPREVVIDEKPSTQIALGRLMRVIWLTPAMDRLWTDAPEQRRRFLDRVTLSFTPGHAEDALGYEKAMRERNRLL
RDEVRDAGWYRALEAQMAETGAALTRNRLDAIARIMAAQEGAGTAFPSASLTLLPGEGSADDPDAESIAARLAEGRGRDM
AAGRTLTGPHRADLGAHWGPQAMPAALSSTGEQKALLLSLILANARALAEESPVLLLDEVAAHLDADRRAALYDEICALR
AQAWLTGTGPELFEALRGRAQFLAVVREAEASRIMGI

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=345, Percent_Identity=27.2463768115942, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 38823; Mature: 38692

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSLLHLTQFRSWARLEIEADHRPVAIHGPNGAGKTNILEAVSMLSPGRGMRGAAPGDQ
CCEEHHHHHHHCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHH
ARKGPEVGWQIRAEIGTHQVLTRALPGQPREVVIDEKPSTQIALGRLMRVIWLTPAMDRL
HCCCCCCCEEEEEHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCHHHHHH
WTDAPEQRRRFLDRVTLSFTPGHAEDALGYEKAMRERNRLLRDEVRDAGWYRALEAQMAE
HCCCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGAALTRNRLDAIARIMAAQEGAGTAFPSASLTLLPGEGSADDPDAESIAARLAEGRGRD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCC
MAAGRTLTGPHRADLGAHWGPQAMPAALSSTGEQKALLLSLILANARALAEESPVLLLDE
CCCCCCCCCCCHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHH
VAAHLDADRRAALYDEICALRAQAWLTGTGPELFEALRGRAQFLAVVREAEASRIMGI
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLSLLHLTQFRSWARLEIEADHRPVAIHGPNGAGKTNILEAVSMLSPGRGMRGAAPGDQ
CEEHHHHHHHCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHH
ARKGPEVGWQIRAEIGTHQVLTRALPGQPREVVIDEKPSTQIALGRLMRVIWLTPAMDRL
HCCCCCCCEEEEEHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCHHHHHH
WTDAPEQRRRFLDRVTLSFTPGHAEDALGYEKAMRERNRLLRDEVRDAGWYRALEAQMAE
HCCCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGAALTRNRLDAIARIMAAQEGAGTAFPSASLTLLPGEGSADDPDAESIAARLAEGRGRD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCC
MAAGRTLTGPHRADLGAHWGPQAMPAALSSTGEQKALLLSLILANARALAEESPVLLLDE
CCCCCCCCCCCHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHH
VAAHLDADRRAALYDEICALRAQAWLTGTGPELFEALRGRAQFLAVVREAEASRIMGI
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]