Click here to switch to the map view.

The map label for this gene is phoA [H]

Identifier: 119358457

GI number: 119358457

Start: 3086482

End: 3087987

Strand: Reverse

Name: phoA [H]

Synonym: Cpha266_2693

Alternate gene names: 119358457

Gene position: 3087987-3086482 (Counterclockwise)

Preceding gene: 119358458

Following gene: 119358456

Centisome position: 98.53

GC content: 53.25

Gene sequence:

>1506_bases
ATGCGACATTTTCTGACGAACAGAACAAAAAGAATCGCGCTGTTTTCATCAGCGCTGTTCGTTCTCATATTGCTGTCGGC
TCCGCTCGATGCCGCATCGCGGAGGACAACGAAACCCGCAGGCGCTCCTCGCTATATCTTTCTCTTTATCGGCGACGGGA
TGGGGCTCGCACAGGCGGCACTCTCCGATGCGATGCGAGAAAGGGGCACCCCTGGGCTGGTGATGAACACATTCCCTTCA
ATCGGCATCGCAACCACCCATGCCGAAAACCGTTTCATTACCGATTCCGGAGCGGCCGGAACAGCGCTTGCTACCGGAAG
CAAGACGAGCATCGGGACTATTTCCATGGCCGCAAATCATAACGATACGCTCAGAACCATTGCCGAAATGGTGAAAGCGA
AAGGCATGAAAGTCGGCATTGTCAGCACTGTCGGCATTAACGATGCAACGCCCGCCTGTTTCTATGCTCATAACGCCAAC
CGGAAAAATGTATACAACATCGCCTTGCAGATGCCCTCAAGCGGGGTTGACTATTTCGGCGGAGGCTACGCCAACGGAAA
CCTTCCGCAAAACCGCAAAAGCGGAACCTCCTTTCGCGGCGATATTGTCGATCTCATGAAATCCCGTAACTATGCTGTTG
TAACCGAAAGAGAGCAGCTCGGAAGCGTTCAGCCCGGAACCAGATGCTGGGCCTATACGGCATATGATGAACAAGGAGCC
ATGAGCTATGCTCTGGATCGCAAGCAGAAAGAGATCTCCCTTGCCGAATTTACCCGCGAAGGTATTCGTCTGCTCGACAA
CCCGAGAGGCTTTTTCATGATGGTTGAAGGGGGAAAAATCGACTGGGCCTGTCATGCCAATGATGCTGCTGCTGTCGCTG
GCGACGTGCGTGACTTTGATCGGGCAATAGCCGAAGCCGTTCTGTTTTATCGAAAGCGCCCGCATCAAACCCTCATTATC
GTAACCGCCGACCACGAATGCGGAGGGTTGACGCTTGGCAATGCGGCACGCGGTTATGCATCGCGGTTTGAGTTGCTGGG
TTACCAGAAAATCTCCGGAGAGCGCTTTGCCGAAAAAGTCGCGACATGGAAAAAAAACCGCAACATATCCTTTCCCATGG
CGCTTGACAGTATAAAAGTCTACTATGGGCTTGGCAATGCCGCAGCCGACTCCGCCCTTGCCCTCTCCTCTGCTGATCGC
CAGTCGCTCAACGATGCCTACTCGTCGAGCATGGCAAGAGAGGTATCGGACAAAAATCGCTACAGCGCACCGGATGCATT
GGCGACAGTCGCGACAGGAATGCTCAACAGGAGGGCCGGAATCGGGTGGACAAGTCCGGTTCATACAGCCCTTCCGGTAC
AGGTTATGGCTCTTGGGGTCGGAGCTTCCGTATTTACCGGGTTTTACGACAATACCGATATAGCAGCTAAAATCATCGCC
GCTTCACAACTCAAGCCCTTCATCGAAAAGCAGAAGAGAAGTAAAAATATTGCAAACAGGCCCTGA

Upstream 100 bases:

>100_bases
GGTGGTTGATGCGAGGAGTTAACGATACTGTAATAGTATGGAACCGAAGGATTCCCTACATTTTCAGAAAATCTGATCAA
CGCTCCAACGGCAACCATTC

Downstream 100 bases:

>100_bases
TAGTACGCCAAAAAATGTAAAAAAGATGCTGTTGGGGAGCGAGAAAAAAGAATTATACTCTTTTCTTCTTTTAGAAATCG
TTAACTTCAGGAAAGATATA

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MRHFLTNRTKRIALFSSALFVLILLSAPLDAASRRTTKPAGAPRYIFLFIGDGMGLAQAALSDAMRERGTPGLVMNTFPS
IGIATTHAENRFITDSGAAGTALATGSKTSIGTISMAANHNDTLRTIAEMVKAKGMKVGIVSTVGINDATPACFYAHNAN
RKNVYNIALQMPSSGVDYFGGGYANGNLPQNRKSGTSFRGDIVDLMKSRNYAVVTEREQLGSVQPGTRCWAYTAYDEQGA
MSYALDRKQKEISLAEFTREGIRLLDNPRGFFMMVEGGKIDWACHANDAAAVAGDVRDFDRAIAEAVLFYRKRPHQTLII
VTADHECGGLTLGNAARGYASRFELLGYQKISGERFAEKVATWKKNRNISFPMALDSIKVYYGLGNAAADSALALSSADR
QSLNDAYSSSMAREVSDKNRYSAPDALATVATGMLNRRAGIGWTSPVHTALPVQVMALGVGASVFTGFYDNTDIAAKIIA
ASQLKPFIEKQKRSKNIANRP

Sequences:

>Translated_501_residues
MRHFLTNRTKRIALFSSALFVLILLSAPLDAASRRTTKPAGAPRYIFLFIGDGMGLAQAALSDAMRERGTPGLVMNTFPS
IGIATTHAENRFITDSGAAGTALATGSKTSIGTISMAANHNDTLRTIAEMVKAKGMKVGIVSTVGINDATPACFYAHNAN
RKNVYNIALQMPSSGVDYFGGGYANGNLPQNRKSGTSFRGDIVDLMKSRNYAVVTEREQLGSVQPGTRCWAYTAYDEQGA
MSYALDRKQKEISLAEFTREGIRLLDNPRGFFMMVEGGKIDWACHANDAAAVAGDVRDFDRAIAEAVLFYRKRPHQTLII
VTADHECGGLTLGNAARGYASRFELLGYQKISGERFAEKVATWKKNRNISFPMALDSIKVYYGLGNAAADSALALSSADR
QSLNDAYSSSMAREVSDKNRYSAPDALATVATGMLNRRAGIGWTSPVHTALPVQVMALGVGASVFTGFYDNTDIAAKIIA
ASQLKPFIEKQKRSKNIANRP
>Mature_501_residues
MRHFLTNRTKRIALFSSALFVLILLSAPLDAASRRTTKPAGAPRYIFLFIGDGMGLAQAALSDAMRERGTPGLVMNTFPS
IGIATTHAENRFITDSGAAGTALATGSKTSIGTISMAANHNDTLRTIAEMVKAKGMKVGIVSTVGINDATPACFYAHNAN
RKNVYNIALQMPSSGVDYFGGGYANGNLPQNRKSGTSFRGDIVDLMKSRNYAVVTEREQLGSVQPGTRCWAYTAYDEQGA
MSYALDRKQKEISLAEFTREGIRLLDNPRGFFMMVEGGKIDWACHANDAAAVAGDVRDFDRAIAEAVLFYRKRPHQTLII
VTADHECGGLTLGNAARGYASRFELLGYQKISGERFAEKVATWKKNRNISFPMALDSIKVYYGLGNAAADSALALSSADR
QSLNDAYSSSMAREVSDKNRYSAPDALATVATGMLNRRAGIGWTSPVHTALPVQVMALGVGASVFTGFYDNTDIAAKIIA
ASQLKPFIEKQKRSKNIANRP

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=329, Percent_Identity=28.8753799392097, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI294660770, Length=327, Percent_Identity=29.0519877675841, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI94721246, Length=339, Percent_Identity=30.0884955752212, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI157266296, Length=339, Percent_Identity=29.7935103244838, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI157266292, Length=339, Percent_Identity=29.4985250737463, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI294660772, Length=289, Percent_Identity=29.0657439446367, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI48994877, Length=338, Percent_Identity=29.585798816568, Blast_Score=101, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6320689, Length=479, Percent_Identity=25.2609603340292, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24651554, Length=352, Percent_Identity=29.2613636363636, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21355151, Length=479, Percent_Identity=24.2171189979123, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI19921912, Length=350, Percent_Identity=28.2857142857143, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI85725200, Length=354, Percent_Identity=27.4011299435028, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI85815833, Length=354, Percent_Identity=27.4011299435028, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355981, Length=321, Percent_Identity=25.2336448598131, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24657835, Length=333, Percent_Identity=27.6276276276276, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18859923, Length=311, Percent_Identity=25.08038585209, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24657842, Length=332, Percent_Identity=27.4096385542169, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 54095; Mature: 54095

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHFLTNRTKRIALFSSALFVLILLSAPLDAASRRTTKPAGAPRYIFLFIGDGMGLAQAA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEEEECCCHHHHHH
LSDAMRERGTPGLVMNTFPSIGIATTHAENRFITDSGAAGTALATGSKTSIGTISMAANH
HHHHHHHCCCCCEEEECCCCCCEEEECCCCEEEECCCCCCCEEECCCCCCCEEEEEECCC
NDTLRTIAEMVKAKGMKVGIVSTVGINDATPACFYAHNANRKNVYNIALQMPSSGVDYFG
CHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCC
GGYANGNLPQNRKSGTSFRGDIVDLMKSRNYAVVTEREQLGSVQPGTRCWAYTAYDEQGA
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEHHHCCCCCCCCEEEEEEEECCCCC
MSYALDRKQKEISLAEFTREGIRLLDNPRGFFMMVEGGKIDWACHANDAAAVAGDVRDFD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEECCCCHHHHHHHHHHH
RAIAEAVLFYRKRPHQTLIIVTADHECGGLTLGNAARGYASRFELLGYQKISGERFAEKV
HHHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCHHHHHHHHHHHCCHHHCHHHHHHHH
ATWKKNRNISFPMALDSIKVYYGLGNAAADSALALSSADRQSLNDAYSSSMAREVSDKNR
HHHHCCCCCCCCEECCCEEEEEECCCHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
YSAPDALATVATGMLNRRAGIGWTSPVHTALPVQVMALGVGASVFTGFYDNTDIAAKIIA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCCEEEEECCCHHHHHCCCCCCCHHHHHHH
ASQLKPFIEKQKRSKNIANRP
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRHFLTNRTKRIALFSSALFVLILLSAPLDAASRRTTKPAGAPRYIFLFIGDGMGLAQAA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEEEECCCHHHHHH
LSDAMRERGTPGLVMNTFPSIGIATTHAENRFITDSGAAGTALATGSKTSIGTISMAANH
HHHHHHHCCCCCEEEECCCCCCEEEECCCCEEEECCCCCCCEEECCCCCCCEEEEEECCC
NDTLRTIAEMVKAKGMKVGIVSTVGINDATPACFYAHNANRKNVYNIALQMPSSGVDYFG
CHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCC
GGYANGNLPQNRKSGTSFRGDIVDLMKSRNYAVVTEREQLGSVQPGTRCWAYTAYDEQGA
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEHHHCCCCCCCCEEEEEEEECCCCC
MSYALDRKQKEISLAEFTREGIRLLDNPRGFFMMVEGGKIDWACHANDAAAVAGDVRDFD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEECCCCHHHHHHHHHHH
RAIAEAVLFYRKRPHQTLIIVTADHECGGLTLGNAARGYASRFELLGYQKISGERFAEKV
HHHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCHHHHHHHHHHHCCHHHCHHHHHHHH
ATWKKNRNISFPMALDSIKVYYGLGNAAADSALALSSADRQSLNDAYSSSMAREVSDKNR
HHHHCCCCCCCCEECCCEEEEEECCCHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
YSAPDALATVATGMLNRRAGIGWTSPVHTALPVQVMALGVGASVFTGFYDNTDIAAKIIA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCCEEEEECCCHHHHHCCCCCCCHHHHHHH
ASQLKPFIEKQKRSKNIANRP
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]