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The map label for this gene is prfA

Identifier: 119358446

GI number: 119358446

Start: 3077025

End: 3078098

Strand: Direct

Name: prfA

Synonym: Cpha266_2682

Alternate gene names: 119358446

Gene position: 3077025-3078098 (Clockwise)

Preceding gene: 119358445

Following gene: 119358447

Centisome position: 98.19

GC content: 54.56

Gene sequence:

>1074_bases
ATGTTTGATAAACTGCAGGCTATAAAGGAAAAACACGAGGATCTTGAGCAGCAGCTTCTTGATCCTCATGCGGCGGAGGA
TCAGAACCGCTTCAGAAAACTGAACAAGGAGTACCGTGATCTCTGTTCCATTGTCGAGGCCTATGACCGGTACGCTCTGG
CTAAAAAACAGCTTGAGGAGTCGCGCCTTCTTTTGAAGAATGAGAAGGAGCAGGATATGCGCGCAATGGTGCAGGATGAA
ATCAGCTCGCTGCAGGAGAGCATCCTTGAACGGGAAAAACAGCTCAAGCTCCTTCTTCTTCCAAAAGATGAAGTGGATTC
CCGCAACGTCATTATCGAGATCAGGGCTGGGACCGGAGGCGAAGAGGCCGCGCTCTTTGCCGCGGATCTGGTGCGAATGT
ATCAGCGGTTCGCCGAACAGCAGGGCTGGAAGTATGAGGTTCTTGATTTTAACGAGAGTTCCGTTCCCGGCGGGTTCAGG
GAGATTGTGCTCGGCGTGAGCGGCCATGACGTCTATGGCACCATGAAATACGAAAGCGGCGTGCACCGGGTTCAGCGCGT
TCCGGATACGGAAACGCAGGGACGTATCCACACCTCTGCCGCAAGCGTGGCCGTGTTGCCGGAAGCGGAAGAGGTCGATG
TTGAGATCCGCAAGGACGATCTTCGGTTTGATACCTACCGAAGCGGTGGAAAGGGTGGCCAGAATGTCAATAAGGTCGAA
ACGGCGGTTCGCATTACCCACCAGCCGAGCGGTATTGTTGTTGCCTGTCAGGAAGAGCGATCCCAGTTGCAGAACAAGGA
GCGGGCAATGAAGATGCTCAGGGCAAAACTCTATGATCGTCAACTTGCCGAGCAGCAGGAGAAACGGGCCGGGCTGCGCC
GATCGATGGTGACGACGGGAGACCGAAGTGCCAAAATCAGAACCTACAACTATCCGCAGTCACGCGTTACCGATCACCGG
ATCGGGTTTACCAGCCATGCTCTTCCGCAAATCATGCAGGGCGACATCAAGGATATCATCGAGGCGCTGAAAATGCACGA
TCAGGCCGAGCGACTGCAGGCCGAACTGGTGTGA

Upstream 100 bases:

>100_bases
AGCAGATCGGCATGCTCCTCCTGTTGTCGCTCTTTGCGTTCATTCTTTTTAACGACATAGTGAATCCGTAAGGACTCGGG
AGCATATTCATTGCAGAACC

Downstream 100 bases:

>100_bases
TGGGTCAGACCGGGATCAAGAGAATTGAGTGTCGCTCTTTTATTGTCTGTTTGTCTGTATATTTTATACGGTAACGAATT
ATGAACGGCAGCCCGAAAGA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MFDKLQAIKEKHEDLEQQLLDPHAAEDQNRFRKLNKEYRDLCSIVEAYDRYALAKKQLEESRLLLKNEKEQDMRAMVQDE
ISSLQESILEREKQLKLLLLPKDEVDSRNVIIEIRAGTGGEEAALFAADLVRMYQRFAEQQGWKYEVLDFNESSVPGGFR
EIVLGVSGHDVYGTMKYESGVHRVQRVPDTETQGRIHTSAASVAVLPEAEEVDVEIRKDDLRFDTYRSGGKGGQNVNKVE
TAVRITHQPSGIVVACQEERSQLQNKERAMKMLRAKLYDRQLAEQQEKRAGLRRSMVTTGDRSAKIRTYNYPQSRVTDHR
IGFTSHALPQIMQGDIKDIIEALKMHDQAERLQAELV

Sequences:

>Translated_357_residues
MFDKLQAIKEKHEDLEQQLLDPHAAEDQNRFRKLNKEYRDLCSIVEAYDRYALAKKQLEESRLLLKNEKEQDMRAMVQDE
ISSLQESILEREKQLKLLLLPKDEVDSRNVIIEIRAGTGGEEAALFAADLVRMYQRFAEQQGWKYEVLDFNESSVPGGFR
EIVLGVSGHDVYGTMKYESGVHRVQRVPDTETQGRIHTSAASVAVLPEAEEVDVEIRKDDLRFDTYRSGGKGGQNVNKVE
TAVRITHQPSGIVVACQEERSQLQNKERAMKMLRAKLYDRQLAEQQEKRAGLRRSMVTTGDRSAKIRTYNYPQSRVTDHR
IGFTSHALPQIMQGDIKDIIEALKMHDQAERLQAELV
>Mature_357_residues
MFDKLQAIKEKHEDLEQQLLDPHAAEDQNRFRKLNKEYRDLCSIVEAYDRYALAKKQLEESRLLLKNEKEQDMRAMVQDE
ISSLQESILEREKQLKLLLLPKDEVDSRNVIIEIRAGTGGEEAALFAADLVRMYQRFAEQQGWKYEVLDFNESSVPGGFR
EIVLGVSGHDVYGTMKYESGVHRVQRVPDTETQGRIHTSAASVAVLPEAEEVDVEIRKDDLRFDTYRSGGKGGQNVNKVE
TAVRITHQPSGIVVACQEERSQLQNKERAMKMLRAKLYDRQLAEQQEKRAGLRRSMVTTGDRSAKIRTYNYPQSRVTDHR
IGFTSHALPQIMQGDIKDIIEALKMHDQAERLQAELV

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=299, Percent_Identity=46.4882943143813, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI34577120, Length=338, Percent_Identity=35.207100591716, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI166795305, Length=196, Percent_Identity=43.8775510204082, Blast_Score=181, Evalue=7e-46,
Organism=Escherichia coli, GI1787462, Length=352, Percent_Identity=46.5909090909091, Blast_Score=321, Evalue=5e-89,
Organism=Escherichia coli, GI2367172, Length=337, Percent_Identity=35.6083086053413, Blast_Score=203, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=315, Percent_Identity=37.4603174603175, Blast_Score=197, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=326, Percent_Identity=38.6503067484663, Blast_Score=223, Evalue=5e-59,
Organism=Drosophila melanogaster, GI19921226, Length=294, Percent_Identity=41.156462585034, Blast_Score=222, Evalue=3e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_CHLPD (A1BJT9)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_913090.1
- ProteinModelPortal:   A1BJT9
- SMR:   A1BJT9
- STRING:   A1BJT9
- GeneID:   4568861
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_2682
- NMPDR:   fig|290317.7.peg.2803
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   GEAGGYK
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41000; Mature: 41000

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDKLQAIKEKHEDLEQQLLDPHAAEDQNRFRKLNKEYRDLCSIVEAYDRYALAKKQLEE
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRLLLKNEKEQDMRAMVQDEISSLQESILEREKQLKLLLLPKDEVDSRNVIIEIRAGTGG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCCCC
EEAALFAADLVRMYQRFAEQQGWKYEVLDFNESSVPGGFREIVLGVSGHDVYGTMKYESG
CHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHEECCCCCCEEEEEHHHHH
VHRVQRVPDTETQGRIHTSAASVAVLPEAEEVDVEIRKDDLRFDTYRSGGKGGQNVNKVE
HHHHHCCCCCCCCCEEEECCCCEEECCCCHHCCEEEECCCCCCHHCCCCCCCCCCHHHHH
TAVRITHQPSGIVVACQEERSQLQNKERAMKMLRAKLYDRQLAEQQEKRAGLRRSMVTTG
HHHEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DRSAKIRTYNYPQSRVTDHRIGFTSHALPQIMQGDIKDIIEALKMHDQAERLQAELV
CCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MFDKLQAIKEKHEDLEQQLLDPHAAEDQNRFRKLNKEYRDLCSIVEAYDRYALAKKQLEE
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRLLLKNEKEQDMRAMVQDEISSLQESILEREKQLKLLLLPKDEVDSRNVIIEIRAGTGG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCCCC
EEAALFAADLVRMYQRFAEQQGWKYEVLDFNESSVPGGFREIVLGVSGHDVYGTMKYESG
CHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHEECCCCCCEEEEEHHHHH
VHRVQRVPDTETQGRIHTSAASVAVLPEAEEVDVEIRKDDLRFDTYRSGGKGGQNVNKVE
HHHHHCCCCCCCCCEEEECCCCEEECCCCHHCCEEEECCCCCCHHCCCCCCCCCCHHHHH
TAVRITHQPSGIVVACQEERSQLQNKERAMKMLRAKLYDRQLAEQQEKRAGLRRSMVTTG
HHHEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DRSAKIRTYNYPQSRVTDHRIGFTSHALPQIMQGDIKDIIEALKMHDQAERLQAELV
CCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA