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The map label for this gene is gmd [H]

Identifier: 119358376

GI number: 119358376

Start: 2994147

End: 2995340

Strand: Reverse

Name: gmd [H]

Synonym: Cpha266_2608

Alternate gene names: 119358376

Gene position: 2995340-2994147 (Counterclockwise)

Preceding gene: 119358377

Following gene: 119358375

Centisome position: 95.58

GC content: 51.34

Gene sequence:

>1194_bases
ATGTCTTTACTCAGCACTCAGGACTCAGCACTCAGCACTTCAAAGCCTCGCGTTGCTCTTATTACCGGCATAACCGGCCA
GGATGGTTCTTATCTGGCGGAGTTTTTGCTTGAAAGGGGTTATGATGTGCATGGCATCAAGCGCAGAGCCAGTTCTTTCA
ATACCCAGCGTATCGACCATCTTTATCAGGATCGCCATGAAATAGAAAAATCTTCTTCACTCAGTACGCAGGAAAAGCGA
GGATCGCTTTTCCTGCATTACGGCGACCTGACTGATAGCAGTAATCTTACTCGCATTATCTCGGAGGTGCAGCCTGATGA
GGTTTATAATCTTGGTGCACAGAGTCATGTTGCGGTGAGCTTTGAGTCGCCTGAGTATACGGCTGATGTTGATGCCATGG
GAACCCTTCGCCTGCTTGAGGCGATCCGTTTTCTGGGGCTTGAAAAGAAAACCCGCTTTTATCAGGCATCCACTTCAGAG
CTGTATGGTCTGGTGCAGGAGATTCCGCAGCGGGAGAGTACGCCTTTTTATCCCCGCAGCCCTTATGCTGTGGCGAAACT
CTATGCTTACTGGATTACGGTGAACTATCGTGAGGCTTACGGGATGTATGCCTGCAATGGCATCCTGTTTAACCATGAGT
CGTCACGAAGGGGAGAGACCTTTGTGACGCGCAAGATCACCCGATGCCTTGCCAATATTACTCAGGGGCTCGAAGAGTGT
CTGTATGTTGGCAACATGAATGCCCTGCGTGACTGGGGTCATGCGAAGGATTATGTGCGCATGCAGTGGATGATGCTGCA
GCAGGAACAGCCGAGGGATTATGTTATTGCGACCGGCGTGCAGTACAGTGTCCGTCAGTTTATCGAAAAGGCTGCCGTAC
AGCTTGGCATCACCATTCGCTGGGAAGGCACCGGTATCAACGAAGTCGGTATTGTTGATTCACTCAGTATTCAGCACTCA
GCACTCAGCACTGGTTCAGTTATTGTTCGGGTCGATCCGCGTTACTTCAGGCCTGCTGAGGTTGAGACCTTGCTTGGGGA
TCCGGCAAAGGCGAAAGCTGATCTTGGCTGGGTACCGGAAATTACGCTTGACGAGATGGTCGAGGAGATGGTAGCGTACG
ATCTCGATCTCGCCCGGCGTCACGCCCTCCTCAAAGCCAATGGTTATACCGTGGAGGTCAGCAAGGAGAGTTGA

Upstream 100 bases:

>100_bases
GAGATTCATCAATGAGTACTGAGAACTGAAAAAAGGCCTCAGCTCTCATTGTTGGAATGCTCGGAGTTCAATACTAACCA
AAACTCTCAGAACGAATAAA

Downstream 100 bases:

>100_bases
TGGGGGATGTTGTGGAAAGTGGGTAGTGGGGAATGGGTAATAGGTACTGGGGAATGGGTTTCATTACGAATCTTTTTCTG
TTGTCATCATCCCTTACGCC

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSLLSTQDSALSTSKPRVALITGITGQDGSYLAEFLLERGYDVHGIKRRASSFNTQRIDHLYQDRHEIEKSSSLSTQEKR
GSLFLHYGDLTDSSNLTRIISEVQPDEVYNLGAQSHVAVSFESPEYTADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSE
LYGLVQEIPQRESTPFYPRSPYAVAKLYAYWITVNYREAYGMYACNGILFNHESSRRGETFVTRKITRCLANITQGLEEC
LYVGNMNALRDWGHAKDYVRMQWMMLQQEQPRDYVIATGVQYSVRQFIEKAAVQLGITIRWEGTGINEVGIVDSLSIQHS
ALSTGSVIVRVDPRYFRPAEVETLLGDPAKAKADLGWVPEITLDEMVEEMVAYDLDLARRHALLKANGYTVEVSKES

Sequences:

>Translated_397_residues
MSLLSTQDSALSTSKPRVALITGITGQDGSYLAEFLLERGYDVHGIKRRASSFNTQRIDHLYQDRHEIEKSSSLSTQEKR
GSLFLHYGDLTDSSNLTRIISEVQPDEVYNLGAQSHVAVSFESPEYTADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSE
LYGLVQEIPQRESTPFYPRSPYAVAKLYAYWITVNYREAYGMYACNGILFNHESSRRGETFVTRKITRCLANITQGLEEC
LYVGNMNALRDWGHAKDYVRMQWMMLQQEQPRDYVIATGVQYSVRQFIEKAAVQLGITIRWEGTGINEVGIVDSLSIQHS
ALSTGSVIVRVDPRYFRPAEVETLLGDPAKAKADLGWVPEITLDEMVEEMVAYDLDLARRHALLKANGYTVEVSKES
>Mature_396_residues
SLLSTQDSALSTSKPRVALITGITGQDGSYLAEFLLERGYDVHGIKRRASSFNTQRIDHLYQDRHEIEKSSSLSTQEKRG
SLFLHYGDLTDSSNLTRIISEVQPDEVYNLGAQSHVAVSFESPEYTADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSEL
YGLVQEIPQRESTPFYPRSPYAVAKLYAYWITVNYREAYGMYACNGILFNHESSRRGETFVTRKITRCLANITQGLEECL
YVGNMNALRDWGHAKDYVRMQWMMLQQEQPRDYVIATGVQYSVRQFIEKAAVQLGITIRWEGTGINEVGIVDSLSIQHSA
LSTGSVIVRVDPRYFRPAEVETLLGDPAKAKADLGWVPEITLDEMVEEMVAYDLDLARRHALLKANGYTVEVSKES

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=367, Percent_Identity=60.7629427792915, Blast_Score=454, Evalue=1e-128,
Organism=Escherichia coli, GI1788366, Length=383, Percent_Identity=77.023498694517, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1788353, Length=321, Percent_Identity=23.98753894081, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI48994969, Length=180, Percent_Identity=31.1111111111111, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786974, Length=361, Percent_Identity=24.9307479224377, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=361, Percent_Identity=62.0498614958449, Blast_Score=457, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17539424, Length=367, Percent_Identity=59.6730245231608, Blast_Score=454, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI133901790, Length=367, Percent_Identity=59.6730245231608, Blast_Score=454, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI133901788, Length=367, Percent_Identity=59.6730245231608, Blast_Score=454, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI133901786, Length=367, Percent_Identity=59.6730245231608, Blast_Score=454, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17539422, Length=367, Percent_Identity=59.6730245231608, Blast_Score=453, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24158427, Length=378, Percent_Identity=57.6719576719577, Blast_Score=456, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 44905; Mature: 44774

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLSTQDSALSTSKPRVALITGITGQDGSYLAEFLLERGYDVHGIKRRASSFNTQRIDH
CCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHH
LYQDRHEIEKSSSLSTQEKRGSLFLHYGDLTDSSNLTRIISEVQPDEVYNLGAQSHVAVS
HHHHHHHHHHHCCCCCHHCCCCEEEEECCCCCCCHHHHHHHHCCCHHHHHCCCCCEEEEE
FESPEYTADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQRESTPFYPRS
ECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCC
PYAVAKLYAYWITVNYREAYGMYACNGILFNHESSRRGETFVTRKITRCLANITQGLEEC
CHHHHEEEEEEEEEEHHHHCCEEEECEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LYVGNMNALRDWGHAKDYVRMQWMMLQQEQPRDYVIATGVQYSVRQFIEKAAVQLGITIR
HHHCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHEEEEEE
WEGTGINEVGIVDSLSIQHSALSTGSVIVRVDPRYFRPAEVETLLGDPAKAKADLGWVPE
ECCCCCCCCCCEECCCHHHHCCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCC
ITLDEMVEEMVAYDLDLARRHALLKANGYTVEVSKES
CCHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCC
>Mature Secondary Structure 
SLLSTQDSALSTSKPRVALITGITGQDGSYLAEFLLERGYDVHGIKRRASSFNTQRIDH
CCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHH
LYQDRHEIEKSSSLSTQEKRGSLFLHYGDLTDSSNLTRIISEVQPDEVYNLGAQSHVAVS
HHHHHHHHHHHCCCCCHHCCCCEEEEECCCCCCCHHHHHHHHCCCHHHHHCCCCCEEEEE
FESPEYTADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQRESTPFYPRS
ECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCC
PYAVAKLYAYWITVNYREAYGMYACNGILFNHESSRRGETFVTRKITRCLANITQGLEEC
CHHHHEEEEEEEEEEHHHHCCEEEECEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LYVGNMNALRDWGHAKDYVRMQWMMLQQEQPRDYVIATGVQYSVRQFIEKAAVQLGITIR
HHHCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHEEEEEE
WEGTGINEVGIVDSLSIQHSALSTGSVIVRVDPRYFRPAEVETLLGDPAKAKADLGWVPE
ECCCCCCCCCCEECCCHHHHCCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCC
ITLDEMVEEMVAYDLDLARRHALLKANGYTVEVSKES
CCHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]