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The map label for this gene is prfB [H]

Identifier: 119358306

GI number: 119358306

Start: 2913751

End: 2914842

Strand: Direct

Name: prfB [H]

Synonym: Cpha266_2538

Alternate gene names: 119358306

Gene position: 2913751-2914842 (Clockwise)

Preceding gene: 119358299

Following gene: 119358307

Centisome position: 92.98

GC content: 52.01

Gene sequence:

>1092_bases
TTGAACCACAAAAAGAAAAACTGCAGGAAATACGTTACCGCCTGGACCAGTTACGGGGGTATCTTTGACGTCGATGAACG
CAAAGAGAAAATAGACGACCTCGAAAAAATCACTGCCGACCCCGGATTCTGGAACGACCAGCAAGAGGCCAACAGGACGC
TCAAGGAGCTGAGCGAACACAAATCGTGGACCGGTGCTTTTGATAAACTCGAAGCGGCAGCAAAAACATCCGGGGAAGAG
CTCACCATAGCCGATGAACTTCAGGACGAATCGTTTGCCGACGAACTCATAGCGTCAATTGCCGCCATTGAAAGCGGCAT
CGCTGCCATTGAGTTCAGAAATATGCTGTCCGGCAAAGACGATCCCGGAAACGCCATCATCACCATCCATGCCGGAGCAG
GCGGAACCGAAGCCCAGGACTGGGCAGAAATGCTCTATCGCATGTACATGCGATGGGCTGAACGCAAAGGATTCAAAGTC
TTTACCGACGATTACCAGGAGGGAGACGGCGCCGGCATCAAAACAGCAACCCTCGAAATTCAGGGCCCTTATGCTTACGG
CTATCTGAAAGCCGAAAACGGAGTACACCGCCTCGTTCGCGTTTCGCCATTCGACTCGAACGCCCGACGACACACCTCCT
TTGCAAGCGTCTATACCTACCCGGAAGCGCCTCCCGATGTCGAAATAGACGTTCGCAAGGAAGACCTCGAACTCTCGACC
TTCAGAAGCGGCGGAAAAGGCGGACAGAACGTCAACAAGGTCGAAACCGCCGTGCGCATCAAGCACATCCCCTCTGGCAT
CGTAGTCGGTTGCCAGCAGGAAAGATCCCAGTTTCAGAACAGGGAACGAGCCATGAAAATGCTGATGGCGCAACTGTACC
AGCGTCAGCGGGAAGAAGAAGATGCCAGAAAACGGGAAATTGAAGGCAAAAAGAAAAAGATCGAATGGGGAAGCCAGATC
AGAAGCTATGTGCTCGACGACCGACGCATCAAGGATCACCGAACCAATTATGAACGGTTCGATGTTGAAACCGTGCTTGA
CGGCGATCTCGATACCTTCATGGAAAAATATCTTTCTGAATTCAGCGACTGA

Upstream 100 bases:

>100_bases
CTCCCCGAAAAGCAGTAACCCGGATAAATCGGGGTATCCGTTCGGTGAGGCAGATAAACTGCCCACACCTTTATAGTCAT
TATCAGCAGCATCATCATGT

Downstream 100 bases:

>100_bases
TGGAAGAGCATCAACCTGGGCCCACCATCAGCTTCGCCCTTATTACCGACATTCACTATGCCGCAACCGGAGATCCCGAC
AGAGATTCCGGTTGCAGCGA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MNHKKKNCRKYVTAWTSYGGIFDVDERKEKIDDLEKITADPGFWNDQQEANRTLKELSEHKSWTGAFDKLEAAAKTSGEE
LTIADELQDESFADELIASIAAIESGIAAIEFRNMLSGKDDPGNAIITIHAGAGGTEAQDWAEMLYRMYMRWAERKGFKV
FTDDYQEGDGAGIKTATLEIQGPYAYGYLKAENGVHRLVRVSPFDSNARRHTSFASVYTYPEAPPDVEIDVRKEDLELST
FRSGGKGGQNVNKVETAVRIKHIPSGIVVGCQQERSQFQNRERAMKMLMAQLYQRQREEEDARKREIEGKKKKIEWGSQI
RSYVLDDRRIKDHRTNYERFDVETVLDGDLDTFMEKYLSEFSD

Sequences:

>Translated_363_residues
MNHKKKNCRKYVTAWTSYGGIFDVDERKEKIDDLEKITADPGFWNDQQEANRTLKELSEHKSWTGAFDKLEAAAKTSGEE
LTIADELQDESFADELIASIAAIESGIAAIEFRNMLSGKDDPGNAIITIHAGAGGTEAQDWAEMLYRMYMRWAERKGFKV
FTDDYQEGDGAGIKTATLEIQGPYAYGYLKAENGVHRLVRVSPFDSNARRHTSFASVYTYPEAPPDVEIDVRKEDLELST
FRSGGKGGQNVNKVETAVRIKHIPSGIVVGCQQERSQFQNRERAMKMLMAQLYQRQREEEDARKREIEGKKKKIEWGSQI
RSYVLDDRRIKDHRTNYERFDVETVLDGDLDTFMEKYLSEFSD
>Mature_363_residues
MNHKKKNCRKYVTAWTSYGGIFDVDERKEKIDDLEKITADPGFWNDQQEANRTLKELSEHKSWTGAFDKLEAAAKTSGEE
LTIADELQDESFADELIASIAAIESGIAAIEFRNMLSGKDDPGNAIITIHAGAGGTEAQDWAEMLYRMYMRWAERKGFKV
FTDDYQEGDGAGIKTATLEIQGPYAYGYLKAENGVHRLVRVSPFDSNARRHTSFASVYTYPEAPPDVEIDVRKEDLELST
FRSGGKGGQNVNKVETAVRIKHIPSGIVVGCQQERSQFQNRERAMKMLMAQLYQRQREEEDARKREIEGKKKKIEWGSQI
RSYVLDDRRIKDHRTNYERFDVETVLDGDLDTFMEKYLSEFSD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=254, Percent_Identity=37.007874015748, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI34577120, Length=306, Percent_Identity=33.3333333333333, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI166795305, Length=149, Percent_Identity=34.8993288590604, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=46.6076696165192, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1787462, Length=247, Percent_Identity=38.8663967611336, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17542784, Length=252, Percent_Identity=34.9206349206349, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=246, Percent_Identity=33.739837398374, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19921226, Length=232, Percent_Identity=39.6551724137931, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41505; Mature: 41505

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHKKKNCRKYVTAWTSYGGIFDVDERKEKIDDLEKITADPGFWNDQQEANRTLKELSEH
CCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
KSWTGAFDKLEAAAKTSGEELTIADELQDESFADELIASIAAIESGIAAIEFRNMLSGKD
CCHHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
DPGNAIITIHAGAGGTEAQDWAEMLYRMYMRWAERKGFKVFTDDYQEGDGAGIKTATLEI
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEEEE
QGPYAYGYLKAENGVHRLVRVSPFDSNARRHTSFASVYTYPEAPPDVEIDVRKEDLELST
ECCEEEEEEECCCCCEEEEEECCCCCCCHHHCCHHHEEECCCCCCCCEEEEECCCCCHHH
FRSGGKGGQNVNKVETAVRIKHIPSGIVVGCQQERSQFQNRERAMKMLMAQLYQRQREEE
HCCCCCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DARKREIEGKKKKIEWGSQIRSYVLDDRRIKDHRTNYERFDVETVLDGDLDTFMEKYLSE
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHCCHHHHHCCCHHHHHHHHHHH
FSD
HCC
>Mature Secondary Structure
MNHKKKNCRKYVTAWTSYGGIFDVDERKEKIDDLEKITADPGFWNDQQEANRTLKELSEH
CCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
KSWTGAFDKLEAAAKTSGEELTIADELQDESFADELIASIAAIESGIAAIEFRNMLSGKD
CCHHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
DPGNAIITIHAGAGGTEAQDWAEMLYRMYMRWAERKGFKVFTDDYQEGDGAGIKTATLEI
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEEEE
QGPYAYGYLKAENGVHRLVRVSPFDSNARRHTSFASVYTYPEAPPDVEIDVRKEDLELST
ECCEEEEEEECCCCCEEEEEECCCCCCCHHHCCHHHEEECCCCCCCCEEEEECCCCCHHH
FRSGGKGGQNVNKVETAVRIKHIPSGIVVGCQQERSQFQNRERAMKMLMAQLYQRQREEE
HCCCCCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DARKREIEGKKKKIEWGSQIRSYVLDDRRIKDHRTNYERFDVETVLDGDLDTFMEKYLSE
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHCCHHHHHCCCHHHHHHHHHHH
FSD
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]