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The map label for this gene is smaIM [H]

Identifier: 119358292

GI number: 119358292

Start: 2888446

End: 2889552

Strand: Reverse

Name: smaIM [H]

Synonym: Cpha266_2524

Alternate gene names: 119358292

Gene position: 2889552-2888446 (Counterclockwise)

Preceding gene: 119358293

Following gene: 119358290

Centisome position: 92.2

GC content: 45.26

Gene sequence:

>1107_bases
ATGAGCAAGTATTTTACCACAGAAGAGGCCGCTTTGTATCTGGGCGTCTCCTCAGCAAGAATCCGGCAGTTTATTCTGGA
AGAGCGCCTGCAGACTGATAAATCCGGCAGAGATCATCTCATTGCGGAATCTGTTCTTTCTGATTTTGCAAAGTTTGGCA
GAAAAAAGGTAGGTCGTCCCTTCCTTGATTCGGGCAACACAGATTTTGTTGGTGTAGAGTCAGAAAGAGCATCAGCGTCA
CACCTTCTCATCAATAGAGATCAGCTTGATGAGGATGGTATTCAGGTGATCAATGGCGATACAAGGGATATCATTAAAAG
CCTTCCAGATAACACGTTCAGATGCGTTGTTACCTCTCCGCCCTATTGGGGTGTAAGGGATTATGGCGTTGAAAATCAGA
TTGGAGCTGAGCCTGACCTTCAGGATTATATCAAGGCTCTTGTTGAAATTTTCTCTGAAGTGCGGCGTGTCCTGCAACCA
GACGGGACTTTCTGGCTGAATATCGGTAATACGTATACTTCAGGCGGAAGAAAATGGCGGCAGGAGGATTCAAAAAATAA
AGGCAGAGCTATGTCGTATCGCCCGCCTACACCTGATGGGTTGAAGAAAAAAGACCTGATCGGCGTAGCATGGATGGTGG
CAATGGCTTGCCAGCTTGACGGGTGGTATTTGAGAAATGACATCATCTGGCACAAACCGAATTGCCAGCCTGAAAGTGTG
AAAGACCGCTTAACGGTAGCTCATGAATATCTTTTTATGTTCTCAAAATCAGAACAATACTACTTTAATCAGGAGGCGAT
CAAGGAGTCGTATACAAACGGAAACGGCTTCAAAAACAAGCGGACAGTATGGTCTATAAATACTGAATCTTGTGCTGAAG
CTCATTTTGCGGTTTTCCCGAAAAATCTGGTAAGGCCATGCATATTAGCCGGATCAGAGGAGCGCGATTTGATTCTTGAT
CCTTTCTATGGAGCCGGGACGGTTGGAATTGTTTCGCAGGAACTCAACAGAAAATGTGTCGGTATTGAAATTAATCCGGA
TTATGTTTACATATCAAGCCGACGCAACGCCCGTGTACAAGGCGCACTTATACTGCCGGAATCGTAA

Upstream 100 bases:

>100_bases
TCCAGCTTATGGTGAAGCGCTTACGCTTACAGATGTAAACATATTAAATGCGTATTGTTTTTTGCAGGAGTTTTTCTAAA
TTAGAAGGAAAACCTTACGC

Downstream 100 bases:

>100_bases
ATTCCCATCGTGCATGCACAATGCAGCGACTTTTAAGAAGATTTTTGAGCTGAAGCTTTCGCTCGCTTCCTCCATCGAAG
TATAGCCGGTATAACTCGGT

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.SmaI; N-4 cytosine-specific methyltransferase SmaI [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSKYFTTEEAALYLGVSSARIRQFILEERLQTDKSGRDHLIAESVLSDFAKFGRKKVGRPFLDSGNTDFVGVESERASAS
HLLINRDQLDEDGIQVINGDTRDIIKSLPDNTFRCVVTSPPYWGVRDYGVENQIGAEPDLQDYIKALVEIFSEVRRVLQP
DGTFWLNIGNTYTSGGRKWRQEDSKNKGRAMSYRPPTPDGLKKKDLIGVAWMVAMACQLDGWYLRNDIIWHKPNCQPESV
KDRLTVAHEYLFMFSKSEQYYFNQEAIKESYTNGNGFKNKRTVWSINTESCAEAHFAVFPKNLVRPCILAGSEERDLILD
PFYGAGTVGIVSQELNRKCVGIEINPDYVYISSRRNARVQGALILPES

Sequences:

>Translated_368_residues
MSKYFTTEEAALYLGVSSARIRQFILEERLQTDKSGRDHLIAESVLSDFAKFGRKKVGRPFLDSGNTDFVGVESERASAS
HLLINRDQLDEDGIQVINGDTRDIIKSLPDNTFRCVVTSPPYWGVRDYGVENQIGAEPDLQDYIKALVEIFSEVRRVLQP
DGTFWLNIGNTYTSGGRKWRQEDSKNKGRAMSYRPPTPDGLKKKDLIGVAWMVAMACQLDGWYLRNDIIWHKPNCQPESV
KDRLTVAHEYLFMFSKSEQYYFNQEAIKESYTNGNGFKNKRTVWSINTESCAEAHFAVFPKNLVRPCILAGSEERDLILD
PFYGAGTVGIVSQELNRKCVGIEINPDYVYISSRRNARVQGALILPES
>Mature_367_residues
SKYFTTEEAALYLGVSSARIRQFILEERLQTDKSGRDHLIAESVLSDFAKFGRKKVGRPFLDSGNTDFVGVESERASASH
LLINRDQLDEDGIQVINGDTRDIIKSLPDNTFRCVVTSPPYWGVRDYGVENQIGAEPDLQDYIKALVEIFSEVRRVLQPD
GTFWLNIGNTYTSGGRKWRQEDSKNKGRAMSYRPPTPDGLKKKDLIGVAWMVAMACQLDGWYLRNDIIWHKPNCQPESVK
DRLTVAHEYLFMFSKSEQYYFNQEAIKESYTNGNGFKNKRTVWSINTESCAEAHFAVFPKNLVRPCILAGSEERDLILDP
FYGAGTVGIVSQELNRKCVGIEINPDYVYISSRRNARVQGALILPES

Specific function: This methylase recognizes the double-stranded sequence CCCGGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the SmaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 41779; Mature: 41647

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYFTTEEAALYLGVSSARIRQFILEERLQTDKSGRDHLIAESVLSDFAKFGRKKVGRP
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
FLDSGNTDFVGVESERASASHLLINRDQLDEDGIQVINGDTRDIIKSLPDNTFRCVVTSP
CCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCEEECCCHHHHHHHCCCCCEEEEEECC
PYWGVRDYGVENQIGAEPDLQDYIKALVEIFSEVRRVLQPDGTFWLNIGNTYTSGGRKWR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEECCCCCHHH
QEDSKNKGRAMSYRPPTPDGLKKKDLIGVAWMVAMACQLDGWYLRNDIIWHKPNCQPESV
HHCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCEEEECCCCCHHHH
KDRLTVAHEYLFMFSKSEQYYFNQEAIKESYTNGNGFKNKRTVWSINTESCAEAHFAVFP
HHHHHHHHHHHHEEECCCCEEECHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHCCC
KNLVRPCILAGSEERDLILDPFYGAGTVGIVSQELNRKCVGIEINPDYVYISSRRNARVQ
HHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHCCEEEEEEECCCEEEEECCCCCEEE
GALILPES
EEEEECCC
>Mature Secondary Structure 
SKYFTTEEAALYLGVSSARIRQFILEERLQTDKSGRDHLIAESVLSDFAKFGRKKVGRP
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
FLDSGNTDFVGVESERASASHLLINRDQLDEDGIQVINGDTRDIIKSLPDNTFRCVVTSP
CCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCEEECCCHHHHHHHCCCCCEEEEEECC
PYWGVRDYGVENQIGAEPDLQDYIKALVEIFSEVRRVLQPDGTFWLNIGNTYTSGGRKWR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEECCCCCHHH
QEDSKNKGRAMSYRPPTPDGLKKKDLIGVAWMVAMACQLDGWYLRNDIIWHKPNCQPESV
HHCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCEEEECCCCCHHHH
KDRLTVAHEYLFMFSKSEQYYFNQEAIKESYTNGNGFKNKRTVWSINTESCAEAHFAVFP
HHHHHHHHHHHHEEECCCCEEECHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHCCC
KNLVRPCILAGSEERDLILDPFYGAGTVGIVSQELNRKCVGIEINPDYVYISSRRNARVQ
HHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHCCEEEEEEECCCEEEEECCCCCEEE
GALILPES
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2690008; 2251121 [H]