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The map label for this gene is leuA [H]

Identifier: 119358246

GI number: 119358246

Start: 2849847

End: 2851529

Strand: Reverse

Name: leuA [H]

Synonym: Cpha266_2478

Alternate gene names: 119358246

Gene position: 2851529-2849847 (Counterclockwise)

Preceding gene: 119358247

Following gene: 119358245

Centisome position: 90.99

GC content: 51.57

Gene sequence:

>1683_bases
ATGAAAACAATGAATTATCATAAGTACACAGCGTATCCCGCTGTCGGAATTGCCAACAGGAGCTGGCCGGATCAAACCAT
AACAAAAGCTCCGATATGGAGCAGCGTGGATTTGCGTGACGGCAATCAGGCGCTTCCCGTTCCTATGAACGTGGATGAAA
AAGTCGCCATGTTTCAGCTTCTCGTCTCTGTTGGGTTCAAGGAGATTGAGGTTGGGTTTCCTTCGGCGTCGGCTACCGAG
TTCGCTTTTGTTCGAAGACTGATTGAAAATGGTCTTATCCCTGACGATGTGACGATTCAGGTGCTGACCCAGGCTCGCGA
GCATCTTATAAGAAAAACTTTCGAGGCCATCAAGGGAGCTCGGCATGCTATCGTGCATCTTTACAATTCGACTTCACGTC
AGCAGAGGGAGCAGGTTTTTCGGATGAACAAGGAAGAGATCAAGGAGATTGCCGTTACAGGTACGGCGCTCGTTCGCCGC
CTCAAAGAGGAGAGCGGTAGCGCAGGGATCCGTTTCGAGTATAGTCCGGAGAGTTTTACCGGGACTGAACTTGAGTATGC
GCTTGAGGTTTGTCATGCTGTTATGGATGCTTGGGGCGCTTCAGCACAGGATAAAATCATTCTCAATTTGCCTTCGACCG
TTGAAATGTCGCGTCCGAACATCTATGCCGACCGCATCGAGTGGTTTTGTCGCAACATCAAGGAGCGCGATGCCGTGCTG
ATCAGCGTTCATGCTCATAATGATCGGGGAACAGCGGTTGCTACCGCCGAGTTTGCCGTCATGGCGGGTGCAGACAGGGT
TGAAGGGGCCCTGTTTGGTAATGGCGAGCGGTGCGGAAATATGGATATCGTGGTTATGGCGCTCAACCTGTTCAGCCAGG
GCGTCGATCCCGAGCTCGATTTCAGCGACCTTCCCCGAATTCGCGAGGTCTATCGCAAGTGTACCCGTATGGATATTCAT
GCGCGTCAACCCTATGCCGGAGAACTTGTCTATACGGCGTTTAGTGGATCGCACCAGGATGCTATCAGCAAGGGTATGAA
GGCGCACAAGATGTCCTCAGACGGAATCTGGGATGTTCCTTATCTGCCGATAGATCCGGGAGATGTCGGGTGCAGTTATG
AAGCGATAGTCCGTATCAACAGTCAATCCGGCAAGGGGGGTGTTGCGTATGTGCTCGAAAAGGATTACGGAATACAGATT
CCGAAATGGATGCAGCCTGATTTTGCTGAAGCCGTTCAGGCTGTTGCAGATCGTACCGGGGAGGAGTTGTCGCCTGAAGA
GATTCATTCCCTGTTTCACAAAGAGTATGTGACGCTTAAGGAGCCTTTTGCGCTTAAGAAATGTCATATCAGTTGGGATG
ACGAAGAACCGGATCGTAATGATGAAGAGGCTACGATTATTGCCTGTGTGGTACAGATGAAGGAGCAGGAGTTCAGGCTT
GATGCAGTCGGTAACGGTCCGGTTGATGCGTTTGTCAAGGGTTTTGTCGCTGAGAGCGGTATCGAGTTCAGCGTTGAGGA
TTATATCGAGCATGCAATTGGTCGTAGCGCCGGCGCGCTTGCTGTTGCCTATGTCAGGATCGGTTGCCCCGATGGCAGGG
TCTCTTATGGCGCCGGAATCGATTCCAATATCAGTCTTGCATCGATCAAGGCGATCGTCAGCGCCCTGAACAGGCTTCCC
TGA

Upstream 100 bases:

>100_bases
TTCATTTATTGAGATTAAGCAACAGTAGATAATAAAGCCGCCGCCCTCCTGGGGTAGGTGGCTTTTTTCAGTTTAAAGGC
AAAAAGGAGAGTGATTGGTT

Downstream 100 bases:

>100_bases
TTCTCGTTGTTTTGGCGGTGGCATTCTTTTTTCTGCTACCGCCAAAACACTTTTCTTCTCTTGATTTGCCTCGCTTTTTG
TAATTTAAATAGAAATAGTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MKTMNYHKYTAYPAVGIANRSWPDQTITKAPIWSSVDLRDGNQALPVPMNVDEKVAMFQLLVSVGFKEIEVGFPSASATE
FAFVRRLIENGLIPDDVTIQVLTQAREHLIRKTFEAIKGARHAIVHLYNSTSRQQREQVFRMNKEEIKEIAVTGTALVRR
LKEESGSAGIRFEYSPESFTGTELEYALEVCHAVMDAWGASAQDKIILNLPSTVEMSRPNIYADRIEWFCRNIKERDAVL
ISVHAHNDRGTAVATAEFAVMAGADRVEGALFGNGERCGNMDIVVMALNLFSQGVDPELDFSDLPRIREVYRKCTRMDIH
ARQPYAGELVYTAFSGSHQDAISKGMKAHKMSSDGIWDVPYLPIDPGDVGCSYEAIVRINSQSGKGGVAYVLEKDYGIQI
PKWMQPDFAEAVQAVADRTGEELSPEEIHSLFHKEYVTLKEPFALKKCHISWDDEEPDRNDEEATIIACVVQMKEQEFRL
DAVGNGPVDAFVKGFVAESGIEFSVEDYIEHAIGRSAGALAVAYVRIGCPDGRVSYGAGIDSNISLASIKAIVSALNRLP

Sequences:

>Translated_560_residues
MKTMNYHKYTAYPAVGIANRSWPDQTITKAPIWSSVDLRDGNQALPVPMNVDEKVAMFQLLVSVGFKEIEVGFPSASATE
FAFVRRLIENGLIPDDVTIQVLTQAREHLIRKTFEAIKGARHAIVHLYNSTSRQQREQVFRMNKEEIKEIAVTGTALVRR
LKEESGSAGIRFEYSPESFTGTELEYALEVCHAVMDAWGASAQDKIILNLPSTVEMSRPNIYADRIEWFCRNIKERDAVL
ISVHAHNDRGTAVATAEFAVMAGADRVEGALFGNGERCGNMDIVVMALNLFSQGVDPELDFSDLPRIREVYRKCTRMDIH
ARQPYAGELVYTAFSGSHQDAISKGMKAHKMSSDGIWDVPYLPIDPGDVGCSYEAIVRINSQSGKGGVAYVLEKDYGIQI
PKWMQPDFAEAVQAVADRTGEELSPEEIHSLFHKEYVTLKEPFALKKCHISWDDEEPDRNDEEATIIACVVQMKEQEFRL
DAVGNGPVDAFVKGFVAESGIEFSVEDYIEHAIGRSAGALAVAYVRIGCPDGRVSYGAGIDSNISLASIKAIVSALNRLP
>Mature_560_residues
MKTMNYHKYTAYPAVGIANRSWPDQTITKAPIWSSVDLRDGNQALPVPMNVDEKVAMFQLLVSVGFKEIEVGFPSASATE
FAFVRRLIENGLIPDDVTIQVLTQAREHLIRKTFEAIKGARHAIVHLYNSTSRQQREQVFRMNKEEIKEIAVTGTALVRR
LKEESGSAGIRFEYSPESFTGTELEYALEVCHAVMDAWGASAQDKIILNLPSTVEMSRPNIYADRIEWFCRNIKERDAVL
ISVHAHNDRGTAVATAEFAVMAGADRVEGALFGNGERCGNMDIVVMALNLFSQGVDPELDFSDLPRIREVYRKCTRMDIH
ARQPYAGELVYTAFSGSHQDAISKGMKAHKMSSDGIWDVPYLPIDPGDVGCSYEAIVRINSQSGKGGVAYVLEKDYGIQI
PKWMQPDFAEAVQAVADRTGEELSPEEIHSLFHKEYVTLKEPFALKKCHISWDDEEPDRNDEEATIIACVVQMKEQEFRL
DAVGNGPVDAFVKGFVAESGIEFSVEDYIEHAIGRSAGALAVAYVRIGCPDGRVSYGAGIDSNISLASIKAIVSALNRLP

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=534, Percent_Identity=26.2172284644195, Blast_Score=124, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324225, Length=563, Percent_Identity=44.582593250444, Blast_Score=504, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6324682, Length=564, Percent_Identity=44.3262411347518, Blast_Score=499, Evalue=1e-142,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 62080; Mature: 62080

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS ; PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTMNYHKYTAYPAVGIANRSWPDQTITKAPIWSSVDLRDGNQALPVPMNVDEKVAMFQL
CCCCCCCEEECCCCEECCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHH
LVSVGFKEIEVGFPSASATEFAFVRRLIENGLIPDDVTIQVLTQAREHLIRKTFEAIKGA
HHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
RHAIVHLYNSTSRQQREQVFRMNKEEIKEIAVTGTALVRRLKEESGSAGIRFEYSPESFT
CEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
GTELEYALEVCHAVMDAWGASAQDKIILNLPSTVEMSRPNIYADRIEWFCRNIKERDAVL
CCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEE
ISVHAHNDRGTAVATAEFAVMAGADRVEGALFGNGERCGNMDIVVMALNLFSQGVDPELD
EEEEECCCCCCEEEEEHHHEEECCCHHCCEEECCCCCCCCCHHEEHHHHHHHCCCCCCCC
FSDLPRIREVYRKCTRMDIHARQPYAGELVYTAFSGSHQDAISKGMKAHKMSSDGIWDVP
HHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCHHHCCCCCCCCCCC
YLPIDPGDVGCSYEAIVRINSQSGKGGVAYVLEKDYGIQIPKWMQPDFAEAVQAVADRTG
EECCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
EELSPEEIHSLFHKEYVTLKEPFALKKCHISWDDEEPDRNDEEATIIACVVQMKEQEFRL
CCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHEEE
DAVGNGPVDAFVKGFVAESGIEFSVEDYIEHAIGRSAGALAVAYVRIGCPDGRVSYGAGI
EECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEECCCC
DSNISLASIKAIVSALNRLP
CCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTMNYHKYTAYPAVGIANRSWPDQTITKAPIWSSVDLRDGNQALPVPMNVDEKVAMFQL
CCCCCCCEEECCCCEECCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHH
LVSVGFKEIEVGFPSASATEFAFVRRLIENGLIPDDVTIQVLTQAREHLIRKTFEAIKGA
HHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
RHAIVHLYNSTSRQQREQVFRMNKEEIKEIAVTGTALVRRLKEESGSAGIRFEYSPESFT
CEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
GTELEYALEVCHAVMDAWGASAQDKIILNLPSTVEMSRPNIYADRIEWFCRNIKERDAVL
CCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEE
ISVHAHNDRGTAVATAEFAVMAGADRVEGALFGNGERCGNMDIVVMALNLFSQGVDPELD
EEEEECCCCCCEEEEEHHHEEECCCHHCCEEECCCCCCCCCHHEEHHHHHHHCCCCCCCC
FSDLPRIREVYRKCTRMDIHARQPYAGELVYTAFSGSHQDAISKGMKAHKMSSDGIWDVP
HHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCHHHCCCCCCCCCCC
YLPIDPGDVGCSYEAIVRINSQSGKGGVAYVLEKDYGIQIPKWMQPDFAEAVQAVADRTG
EECCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
EELSPEEIHSLFHKEYVTLKEPFALKKCHISWDDEEPDRNDEEATIIACVVQMKEQEFRL
CCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHEEE
DAVGNGPVDAFVKGFVAESGIEFSVEDYIEHAIGRSAGALAVAYVRIGCPDGRVSYGAGI
EECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEECCCC
DSNISLASIKAIVSALNRLP
CCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]