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The map label for this gene is clpB2 [H]

Identifier: 119358217

GI number: 119358217

Start: 2822951

End: 2824279

Strand: Reverse

Name: clpB2 [H]

Synonym: Cpha266_2449

Alternate gene names: 119358217

Gene position: 2824279-2822951 (Counterclockwise)

Preceding gene: 119358218

Following gene: 119358216

Centisome position: 90.12

GC content: 50.87

Gene sequence:

>1329_bases
ATGCAGTTTGACCCCAACAAATTTACCGTAAAAGCACAAGAGGCGCTTCAGGCTGCCGCTACGCTTGCCGGCAGTCGACA
GCATCAGCAGGTCGAGCCGGAACATCTGCTCTTTTCGGTACTTGACGATAGTTCAAGTATTGCTGTACAGATTGCCCAGA
AACTTGAGGTTTCTGTTGAGAATCTTCTGTCGGCAGTAGAAAGGGAACTTGACAGAATTCCGAAGGTTACCGGGGCATCG
GCGTCCGGTCAGTATATTTCGCAGAATCTCGGCAAGGTGTTTGACGTGGCGCTTAAGGAGGCTGAATCTCTCAAAGACGA
TTACATCAGTTCAGAGCACCTTTTTATTGCTATGAGCGAATCGGGCGTCAAGGTTTCCCGTATGCTGCAGGATGCCGGTA
TTAACAGGAACTCCATTCTTAAAGTGCTTGCAACCATAAGAGGTTCGCAACGGGTAACCAGCCAGACAGCCGAAGATTCA
TACAATTCCCTGAAAAAGTATTCGCGCAATCTTAATGATGAGGTGCGCAAAGGAAAGCTTGATCCTGTTATCGGTCGAGA
TGATGAGATCAGGCGCGTTTTGCAGATTCTCAGTCGTCGCACCAAGAACAATCCTGTGCTGATAGGAGATCCGGGAGTTG
GAAAGACCGCACTTGTTGAAGGGATTGCCCAGCGTATTGTAGCCGGCGATGTTCCCGAGAATCTCAAGAGTAAGCAGATT
GCCGCGCTTGATATTGCGCAGCTTGTCGCCGGAGCAAAATTTCGCGGCGAGTTTGAGGAGAGGCTCAAAGCGGTTGTCAA
GGAGGTGCAGTCGGCTGCGGGGGAGATTATTCTGTTTATTGACGAAATTCATCTTCTTGTCGGGGCCGGTTCGGCGGAGG
GGTCAATGGACGCAGCCAATATCCTCAAGCCCGCTCTTGCTCGCGGTGAATTGCGCTGCATCGGCGCAACCACGCTTGAC
GAGTATCGCAAACATATTGAGAAGGATGCTGCTCTTGAGCGTCGGTTCCAGACGGTTCTGGTTGATCAGCCGAGTGTTGA
AGACACCGTTTCAATTCTGCGCGGTTTGAAAGAGAAATATGAAATTCATCATGGAGTACGGATCAAGGATGCCGCTCTTG
TTGCGGCGGCAGAGCTCTCGGATCGATATATTGCTGACCGGTTTCTGCCGGATAAGGCCATCGATCTTATTGATGAGGCA
TCTTCAAGGCTCAGGCTCGAAATCGACAGCAGCCCGGAAGAGCTTGATCGGCTGAACCGCGAGATTAGACGTCTCGAAAT
CGAACGTGAGGCGCTGAAGCGCGAAATCGAAGCTAATCATGGTTCCTGA

Upstream 100 bases:

>100_bases
CTCTATTGGGAGGTCTCTCATCCGATTCTTTATTTTGTGAGGTGGTGGGGGACGGAAACAGAAAACACATTTGCTTGTCA
ACAGAAAAATCGATGCGACC

Downstream 100 bases:

>100_bases
TTCATCGTTTGAGCGGGTAGCGTGCGGTTTTTCCGTTCTGAAACGTTTTTTATTCCCACCGGATTTGTTGCCAGCAAAAT
TGTTGGCAACTTTTTTTAGG

Product: ATPase central domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MQFDPNKFTVKAQEALQAAATLAGSRQHQQVEPEHLLFSVLDDSSSIAVQIAQKLEVSVENLLSAVERELDRIPKVTGAS
ASGQYISQNLGKVFDVALKEAESLKDDYISSEHLFIAMSESGVKVSRMLQDAGINRNSILKVLATIRGSQRVTSQTAEDS
YNSLKKYSRNLNDEVRKGKLDPVIGRDDEIRRVLQILSRRTKNNPVLIGDPGVGKTALVEGIAQRIVAGDVPENLKSKQI
AALDIAQLVAGAKFRGEFEERLKAVVKEVQSAAGEIILFIDEIHLLVGAGSAEGSMDAANILKPALARGELRCIGATTLD
EYRKHIEKDAALERRFQTVLVDQPSVEDTVSILRGLKEKYEIHHGVRIKDAALVAAAELSDRYIADRFLPDKAIDLIDEA
SSRLRLEIDSSPEELDRLNREIRRLEIEREALKREIEANHGS

Sequences:

>Translated_442_residues
MQFDPNKFTVKAQEALQAAATLAGSRQHQQVEPEHLLFSVLDDSSSIAVQIAQKLEVSVENLLSAVERELDRIPKVTGAS
ASGQYISQNLGKVFDVALKEAESLKDDYISSEHLFIAMSESGVKVSRMLQDAGINRNSILKVLATIRGSQRVTSQTAEDS
YNSLKKYSRNLNDEVRKGKLDPVIGRDDEIRRVLQILSRRTKNNPVLIGDPGVGKTALVEGIAQRIVAGDVPENLKSKQI
AALDIAQLVAGAKFRGEFEERLKAVVKEVQSAAGEIILFIDEIHLLVGAGSAEGSMDAANILKPALARGELRCIGATTLD
EYRKHIEKDAALERRFQTVLVDQPSVEDTVSILRGLKEKYEIHHGVRIKDAALVAAAELSDRYIADRFLPDKAIDLIDEA
SSRLRLEIDSSPEELDRLNREIRRLEIEREALKREIEANHGS
>Mature_442_residues
MQFDPNKFTVKAQEALQAAATLAGSRQHQQVEPEHLLFSVLDDSSSIAVQIAQKLEVSVENLLSAVERELDRIPKVTGAS
ASGQYISQNLGKVFDVALKEAESLKDDYISSEHLFIAMSESGVKVSRMLQDAGINRNSILKVLATIRGSQRVTSQTAEDS
YNSLKKYSRNLNDEVRKGKLDPVIGRDDEIRRVLQILSRRTKNNPVLIGDPGVGKTALVEGIAQRIVAGDVPENLKSKQI
AALDIAQLVAGAKFRGEFEERLKAVVKEVQSAAGEIILFIDEIHLLVGAGSAEGSMDAANILKPALARGELRCIGATTLD
EYRKHIEKDAALERRFQTVLVDQPSVEDTVSILRGLKEKYEIHHGVRIKDAALVAAAELSDRYIADRFLPDKAIDLIDEA
SSRLRLEIDSSPEELDRLNREIRRLEIEREALKREIEANHGS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Escherichia coli, GI1788943, Length=430, Percent_Identity=54.1860465116279, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1787109, Length=416, Percent_Identity=39.1826923076923, Blast_Score=275, Evalue=5e-75,
Organism=Saccharomyces cerevisiae, GI6320464, Length=275, Percent_Identity=64.7272727272727, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6323002, Length=442, Percent_Identity=43.8914027149321, Blast_Score=355, Evalue=6e-99,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF02861 Clp_N [H]

EC number: NA

Molecular weight: Translated: 48810; Mature: 48810

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00870 CLPAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDPNKFTVKAQEALQAAATLAGSRQHQQVEPEHLLFSVLDDSSSIAVQIAQKLEVSVE
CCCCCCCEEEHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NLLSAVERELDRIPKVTGASASGQYISQNLGKVFDVALKEAESLKDDYISSEHLFIAMSE
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
SGVKVSRMLQDAGINRNSILKVLATIRGSQRVTSQTAEDSYNSLKKYSRNLNDEVRKGKL
CCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
DPVIGRDDEIRRVLQILSRRTKNNPVLIGDPGVGKTALVEGIAQRIVAGDVPENLKSKQI
CCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
AALDIAQLVAGAKFRGEFEERLKAVVKEVQSAAGEIILFIDEIHLLVGAGSAEGSMDAAN
HHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHEECCCCCCCCCHHH
ILKPALARGELRCIGATTLDEYRKHIEKDAALERRFQTVLVDQPSVEDTVSILRGLKEKY
HHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EIHHGVRIKDAALVAAAELSDRYIADRFLPDKAIDLIDEASSRLRLEIDSSPEELDRLNR
HHHCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
EIRRLEIEREALKREIEANHGS
HHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQFDPNKFTVKAQEALQAAATLAGSRQHQQVEPEHLLFSVLDDSSSIAVQIAQKLEVSVE
CCCCCCCEEEHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NLLSAVERELDRIPKVTGASASGQYISQNLGKVFDVALKEAESLKDDYISSEHLFIAMSE
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
SGVKVSRMLQDAGINRNSILKVLATIRGSQRVTSQTAEDSYNSLKKYSRNLNDEVRKGKL
CCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
DPVIGRDDEIRRVLQILSRRTKNNPVLIGDPGVGKTALVEGIAQRIVAGDVPENLKSKQI
CCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
AALDIAQLVAGAKFRGEFEERLKAVVKEVQSAAGEIILFIDEIHLLVGAGSAEGSMDAAN
HHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHEECCCCCCCCCHHH
ILKPALARGELRCIGATTLDEYRKHIEKDAALERRFQTVLVDQPSVEDTVSILRGLKEKY
HHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EIHHGVRIKDAALVAAAELSDRYIADRFLPDKAIDLIDEASSRLRLEIDSSPEELDRLNR
HHHCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
EIRRLEIEREALKREIEANHGS
HHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]