Click here to switch to the map view.

The map label for this gene is gatB

Identifier: 119358215

GI number: 119358215

Start: 2820669

End: 2822096

Strand: Reverse

Name: gatB

Synonym: Cpha266_2447

Alternate gene names: 119358215

Gene position: 2822096-2820669 (Counterclockwise)

Preceding gene: 119358216

Following gene: 119358213

Centisome position: 90.05

GC content: 49.02

Gene sequence:

>1428_bases
ATGAAGTATGAATTAGTTGTGGGCCTTGAGGTCCATTGTCAGCTCAATACCAACAGCAAAGCTTTTTGCGGTTGTTCAGC
ACTGTTCGGTAAACCGGCCAATACGAACGTTTGTCCGGTTTGCCTCGCTCTTCCGGGCGCTTTGCCGGTACTGAACCGGC
GCGTTGTTGAAGATGCTGTAAAGATCGGTCTGGCAACAGGGTGTTCGGTTGCATCGCATTCAATTCTGGCCCGTAAAAAC
TATTTTTATCCCGATCTTCCCAAGGGGTATCAGATTTCGCAGTATGAAGAGCCGATCTGCAGCGAAGGAATGATTCGTAT
TGATGTCGGTGAGGGTCAACGGGATATCCGTCTGATCAGGATCCATATTGAGGAGGATGCCGGAAAGTCGATTCATGATA
TCGGTGAAGATACCTATATCGATGTGAATCGAAGCGGCGTTCCTCTGCTTGAAATTGTCAGCTATCCTGATATGCGTACG
GCAAAAGAGGCGTCGGCTTATCTGCAAAAGGTTCGCCAGATTGTGAAATATCTGGGGATTTCGGATGGTAACATGGAAGA
GGGAAGTCTTCGCTGTGACGCAAACGTTTCAATCCGTCCGTATGGTTCCGAGGAGTATGGTACCCGTACCGAAATCAAGA
ATATGAACTCATTTAAAAACGTTGAGAAGGCTATTGAGTTCGAGGCGAACCGACACAGCGAGATTATTGATGCCGGCGGT
GCTATTGTTCAGGAGACCAGGCTCTGGGACGCCGACAAGGGTGAAACCCGTTCGATGAGGGGCAAGGAGTTCGCCCATGA
TTACCGGTATTTTCCCGATCCCGATCTTGTTCCCGTTCTTGTTGACGATGATATGCTTGAACGGATGCGTCTTGAGCTTC
CCGAGTTTCCGGAAGATCGCGCAGCTCGTTTTGTTTCCGAGTTTGCCATTCCGGCTTATGATGCCGCAGTACTGACTGTC
GATCGCGAAGTTGCCGACTATTTTGAAGAGACAGTGAGGGTGTCAGGCGATGCCAAGGTCGCTTCAAACTGGGTTATGGG
TGAGGTTATGCGCACTCTGAAAGAGAAGTATCTGGATATTGCTGAATTCGGAATTTCATCGCTGCGTCTTGGTGAACTTA
TCCGGCTTATCGGTGCGGGAACGATCAGTAACACTATAGCAAAACAGGTGTTTGAAATAATGATGTCTGACGAAGCCGCT
GCTGAAGTGATCGTCACGCGGGAAGGTCTTGCGCAGGTTTCGGATTTTGGCGCAATCGATATGGTTCTTCAGGAGGTGCT
CGATGCCAATCCAGGGCAGCTTGCCGATTATCGCGCAGGAAAAACAAAGTTGTTCGGCTTTTTTGTTGGTCAGTGCATGG
CCAGGATGAAAGGGAAGGGCAATCCTGCAATAGTGAACGATCTTTTACAGAAAAAGCTTGCAGGATAG

Upstream 100 bases:

>100_bases
ACTGTAGGCGGACAGAAGTTCAGCTACAGATCGCGCTGATAATCGGCAGGGTGAAGTGGGTCTTTATCCTGAACGGGTTT
ATAAACGATATTATTGCAGT

Downstream 100 bases:

>100_bases
CTTTTTTCAGATTTCCGAGTCAAAGAGATCTCTGTTTCGGGCAGTGTCCGGCCAGTTTTTTGCCAGCTCGGGATTAATGA
TTTCGATTTTTTTGCGGGCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKYELVVGLEVHCQLNTNSKAFCGCSALFGKPANTNVCPVCLALPGALPVLNRRVVEDAVKIGLATGCSVASHSILARKN
YFYPDLPKGYQISQYEEPICSEGMIRIDVGEGQRDIRLIRIHIEEDAGKSIHDIGEDTYIDVNRSGVPLLEIVSYPDMRT
AKEASAYLQKVRQIVKYLGISDGNMEEGSLRCDANVSIRPYGSEEYGTRTEIKNMNSFKNVEKAIEFEANRHSEIIDAGG
AIVQETRLWDADKGETRSMRGKEFAHDYRYFPDPDLVPVLVDDDMLERMRLELPEFPEDRAARFVSEFAIPAYDAAVLTV
DREVADYFEETVRVSGDAKVASNWVMGEVMRTLKEKYLDIAEFGISSLRLGELIRLIGAGTISNTIAKQVFEIMMSDEAA
AEVIVTREGLAQVSDFGAIDMVLQEVLDANPGQLADYRAGKTKLFGFFVGQCMARMKGKGNPAIVNDLLQKKLAG

Sequences:

>Translated_475_residues
MKYELVVGLEVHCQLNTNSKAFCGCSALFGKPANTNVCPVCLALPGALPVLNRRVVEDAVKIGLATGCSVASHSILARKN
YFYPDLPKGYQISQYEEPICSEGMIRIDVGEGQRDIRLIRIHIEEDAGKSIHDIGEDTYIDVNRSGVPLLEIVSYPDMRT
AKEASAYLQKVRQIVKYLGISDGNMEEGSLRCDANVSIRPYGSEEYGTRTEIKNMNSFKNVEKAIEFEANRHSEIIDAGG
AIVQETRLWDADKGETRSMRGKEFAHDYRYFPDPDLVPVLVDDDMLERMRLELPEFPEDRAARFVSEFAIPAYDAAVLTV
DREVADYFEETVRVSGDAKVASNWVMGEVMRTLKEKYLDIAEFGISSLRLGELIRLIGAGTISNTIAKQVFEIMMSDEAA
AEVIVTREGLAQVSDFGAIDMVLQEVLDANPGQLADYRAGKTKLFGFFVGQCMARMKGKGNPAIVNDLLQKKLAG
>Mature_475_residues
MKYELVVGLEVHCQLNTNSKAFCGCSALFGKPANTNVCPVCLALPGALPVLNRRVVEDAVKIGLATGCSVASHSILARKN
YFYPDLPKGYQISQYEEPICSEGMIRIDVGEGQRDIRLIRIHIEEDAGKSIHDIGEDTYIDVNRSGVPLLEIVSYPDMRT
AKEASAYLQKVRQIVKYLGISDGNMEEGSLRCDANVSIRPYGSEEYGTRTEIKNMNSFKNVEKAIEFEANRHSEIIDAGG
AIVQETRLWDADKGETRSMRGKEFAHDYRYFPDPDLVPVLVDDDMLERMRLELPEFPEDRAARFVSEFAIPAYDAAVLTV
DREVADYFEETVRVSGDAKVASNWVMGEVMRTLKEKYLDIAEFGISSLRLGELIRLIGAGTISNTIAKQVFEIMMSDEAA
AEVIVTREGLAQVSDFGAIDMVLQEVLDANPGQLADYRAGKTKLFGFFVGQCMARMKGKGNPAIVNDLLQKKLAG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=496, Percent_Identity=35.0806451612903, Blast_Score=302, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI115534094, Length=304, Percent_Identity=39.1447368421053, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6319391, Length=508, Percent_Identity=30.9055118110236, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI45550808, Length=491, Percent_Identity=33.6048879837067, Blast_Score=284, Evalue=8e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_CHLPD (A1BJ58)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_912859.1
- ProteinModelPortal:   A1BJ58
- SMR:   A1BJ58
- STRING:   A1BJ58
- GeneID:   4568875
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_2447
- NMPDR:   fig|290317.7.peg.2561
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 52670; Mature: 52670

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYELVVGLEVHCQLNTNSKAFCGCSALFGKPANTNVCPVCLALPGALPVLNRRVVEDAV
CCEEEEEEEEEEEEECCCCCCEECHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
KIGLATGCSVASHSILARKNYFYPDLPKGYQISQYEEPICSEGMIRIDVGEGQRDIRLIR
HHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEE
IHIEEDAGKSIHDIGEDTYIDVNRSGVPLLEIVSYPDMRTAKEASAYLQKVRQIVKYLGI
EEEECCCCCHHHHCCCCCEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
SDGNMEEGSLRCDANVSIRPYGSEEYGTRTEIKNMNSFKNVEKAIEFEANRHSEIIDAGG
CCCCCCCCCEEECCCEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCHHHHCCC
AIVQETRLWDADKGETRSMRGKEFAHDYRYFPDPDLVPVLVDDDMLERMRLELPEFPEDR
CCEEHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECHHHHHHHHHCCCCCCHHH
AARFVSEFAIPAYDAAVLTVDREVADYFEETVRVSGDAKVASNWVMGEVMRTLKEKYLDI
HHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AEFGISSLRLGELIRLIGAGTISNTIAKQVFEIMMSDEAAAEVIVTREGLAQVSDFGAID
HHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHEEEEEHHHHHHHHCCCHHH
MVLQEVLDANPGQLADYRAGKTKLFGFFVGQCMARMKGKGNPAIVNDLLQKKLAG
HHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYELVVGLEVHCQLNTNSKAFCGCSALFGKPANTNVCPVCLALPGALPVLNRRVVEDAV
CCEEEEEEEEEEEEECCCCCCEECHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
KIGLATGCSVASHSILARKNYFYPDLPKGYQISQYEEPICSEGMIRIDVGEGQRDIRLIR
HHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEE
IHIEEDAGKSIHDIGEDTYIDVNRSGVPLLEIVSYPDMRTAKEASAYLQKVRQIVKYLGI
EEEECCCCCHHHHCCCCCEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
SDGNMEEGSLRCDANVSIRPYGSEEYGTRTEIKNMNSFKNVEKAIEFEANRHSEIIDAGG
CCCCCCCCCEEECCCEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCHHHHCCC
AIVQETRLWDADKGETRSMRGKEFAHDYRYFPDPDLVPVLVDDDMLERMRLELPEFPEDR
CCEEHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECHHHHHHHHHCCCCCCHHH
AARFVSEFAIPAYDAAVLTVDREVADYFEETVRVSGDAKVASNWVMGEVMRTLKEKYLDI
HHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AEFGISSLRLGELIRLIGAGTISNTIAKQVFEIMMSDEAAAEVIVTREGLAQVSDFGAID
HHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHEEEEEHHHHHHHHCCCHHH
MVLQEVLDANPGQLADYRAGKTKLFGFFVGQCMARMKGKGNPAIVNDLLQKKLAG
HHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA