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The map label for this gene is purA

Identifier: 119358158

GI number: 119358158

Start: 2777878

End: 2779182

Strand: Reverse

Name: purA

Synonym: Cpha266_2390

Alternate gene names: 119358158

Gene position: 2779182-2777878 (Counterclockwise)

Preceding gene: 119358159

Following gene: 119358157

Centisome position: 88.68

GC content: 49.43

Gene sequence:

>1305_bases
GTGGAATCACCATCATTCAGTAAACCAGTCCAGTCAGCAACCGTGATTGTCGGAACACAGTTCGGTGATGAAGGGAAAGG
GAAACTCGTTGACTATCTGTCGGATAAATACGATATCGTCGTCCGCTATCAGGGCGGAGCCAATGCAGGCCATACCATCT
GTTTCGACAATAAAACAATTGTTCTCCATCTGCTTCCTTCCGGTATTTTTCATGAAGGGTGCGTCTGTGTGATCGGTAAC
GGTGTTGTCATTGATCCTGCTGCGTTGCTTGATGAAATCCGCAAAGTTGAGGAACTGGGCTATGAGGTAAAAGGTCGTCT
CTTTATCAGTCATAACGCGCACCTCATCATGCCCTACCACAAGCGGCTTGATTCGTTGCACGAAGATGCCCAGGGCGACC
AGAAAATAGGAACGACGGGAAGGGGAATCGGCCCGAGTTATGAGGACAAATTTGCACGAAAAGGGATCAGGGTTGTCGAT
CTTCTCAATCCCGAAGTGCTTCAGGAAAAACTCAGAGATAACCTTGCAGCTAAAAACAAGCTTCTGAAAACGATCTACGA
AAAGGAAGAGTTCGATATCGACGCAATGGTCAAGGAGTATGCCGAATTCGACAAGATTATTGATCCTTATGTCACCAATA
CGCAGCTCTACCTCAATCGACAGCTCAAGGCGGGTAAAACCGTATTGCTCGAAGGAGCCCAGGGTTGTCTGCTCGATGTC
GATCACGGAACCTATCCCTATGTTACCTCGTCCAATCCCACCTCAGGAGGTGCCTGCACCGGATCGGGAATTGCGCCGAA
CTATATCGGCAAAGTCATAGGTATCTGCAAGGCATACATGACCAGAGTTGGTAACGGGGCATTTCCTTCAGAGCTGTCAG
ATGAAACAGGTGAGCGCCTTGGCCAGATCGGTCATGAATTCGGAGCGACAACCGGAAGAAAGCGACGGTGCGGATGGATT
GATCTTGTCGCGCTGCGCTACTCTCTTGCCGTTAACGGCGTCACGGAAATTGCCCTGACCAAACTTGATGTGCTCGATAC
CTTTGAAGAGATCAGCGTCTGTACCTCCTATATGCTGGATGGAAAAGAGATTCAGGATTTTCCTACCGATCATCAGACGC
TGTCGAGAGTGACGCCGGTGTTCAAAACAATGAAAGGCTGGAAGGCTACCAACGCCGACGCACGAACCTTTGACGAAATG
CGTCCCGAAGCAAGGAACTATGTAACCTTTCTTGAAGAGGAGCTGCAGGTTCCGGTTACCTTTATTTCTGTAGGCCCCGG
CCGTGACGAAACGGTATTCAGATAA

Upstream 100 bases:

>100_bases
TTGAATGCCGAATTTATTGTAAATTATTCATCTTTTTCATCAAAAGCCAGACTTGAACTTCTGGCGCATCTTGCTCAATA
CATCCGTTAAAACAATCGTC

Downstream 100 bases:

>100_bases
CCTTCAGGTTTATTTGCTATGGCATTGATGGATATCACCGTTATTCCCCTTGACAACAGAGATGGCGGCTTGAGTGATTT
CGTGGCAGGATTGCAGGAGC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MESPSFSKPVQSATVIVGTQFGDEGKGKLVDYLSDKYDIVVRYQGGANAGHTICFDNKTIVLHLLPSGIFHEGCVCVIGN
GVVIDPAALLDEIRKVEELGYEVKGRLFISHNAHLIMPYHKRLDSLHEDAQGDQKIGTTGRGIGPSYEDKFARKGIRVVD
LLNPEVLQEKLRDNLAAKNKLLKTIYEKEEFDIDAMVKEYAEFDKIIDPYVTNTQLYLNRQLKAGKTVLLEGAQGCLLDV
DHGTYPYVTSSNPTSGGACTGSGIAPNYIGKVIGICKAYMTRVGNGAFPSELSDETGERLGQIGHEFGATTGRKRRCGWI
DLVALRYSLAVNGVTEIALTKLDVLDTFEEISVCTSYMLDGKEIQDFPTDHQTLSRVTPVFKTMKGWKATNADARTFDEM
RPEARNYVTFLEEELQVPVTFISVGPGRDETVFR

Sequences:

>Translated_434_residues
MESPSFSKPVQSATVIVGTQFGDEGKGKLVDYLSDKYDIVVRYQGGANAGHTICFDNKTIVLHLLPSGIFHEGCVCVIGN
GVVIDPAALLDEIRKVEELGYEVKGRLFISHNAHLIMPYHKRLDSLHEDAQGDQKIGTTGRGIGPSYEDKFARKGIRVVD
LLNPEVLQEKLRDNLAAKNKLLKTIYEKEEFDIDAMVKEYAEFDKIIDPYVTNTQLYLNRQLKAGKTVLLEGAQGCLLDV
DHGTYPYVTSSNPTSGGACTGSGIAPNYIGKVIGICKAYMTRVGNGAFPSELSDETGERLGQIGHEFGATTGRKRRCGWI
DLVALRYSLAVNGVTEIALTKLDVLDTFEEISVCTSYMLDGKEIQDFPTDHQTLSRVTPVFKTMKGWKATNADARTFDEM
RPEARNYVTFLEEELQVPVTFISVGPGRDETVFR
>Mature_434_residues
MESPSFSKPVQSATVIVGTQFGDEGKGKLVDYLSDKYDIVVRYQGGANAGHTICFDNKTIVLHLLPSGIFHEGCVCVIGN
GVVIDPAALLDEIRKVEELGYEVKGRLFISHNAHLIMPYHKRLDSLHEDAQGDQKIGTTGRGIGPSYEDKFARKGIRVVD
LLNPEVLQEKLRDNLAAKNKLLKTIYEKEEFDIDAMVKEYAEFDKIIDPYVTNTQLYLNRQLKAGKTVLLEGAQGCLLDV
DHGTYPYVTSSNPTSGGACTGSGIAPNYIGKVIGICKAYMTRVGNGAFPSELSDETGERLGQIGHEFGATTGRKRRCGWI
DLVALRYSLAVNGVTEIALTKLDVLDTFEEISVCTSYMLDGKEIQDFPTDHQTLSRVTPVFKTMKGWKATNADARTFDEM
RPEARNYVTFLEEELQVPVTFISVGPGRDETVFR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=424, Percent_Identity=46.2264150943396, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI34577063, Length=430, Percent_Identity=45.3488372093023, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=45.1776649746193, Blast_Score=330, Evalue=1e-90,
Organism=Escherichia coli, GI1790620, Length=420, Percent_Identity=47.6190476190476, Blast_Score=400, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25146553, Length=439, Percent_Identity=44.1913439635535, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25146555, Length=433, Percent_Identity=44.5727482678984, Blast_Score=358, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6324109, Length=433, Percent_Identity=42.9561200923788, Blast_Score=359, Evalue=1e-100,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=44.8931116389549, Blast_Score=368, Evalue=1e-102,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_CHLPD (A1BJ01)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_912802.1
- ProteinModelPortal:   A1BJ01
- SMR:   A1BJ01
- STRING:   A1BJ01
- GeneID:   4568612
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_2390
- NMPDR:   fig|290317.7.peg.2505
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47964; Mature: 47964

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 23-23 ACT_SITE 51-51 BINDING 139-139 BINDING 153-153 BINDING 234-234 BINDING 249-249 BINDING 313-313 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESPSFSKPVQSATVIVGTQFGDEGKGKLVDYLSDKYDIVVRYQGGANAGHTICFDNKTI
CCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCEEEECCCEE
VLHLLPSGIFHEGCVCVIGNGVVIDPAALLDEIRKVEELGYEVKGRLFISHNAHLIMPYH
EEEECCCCCCCCCCEEEECCCEEECHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEHHH
KRLDSLHEDAQGDQKIGTTGRGIGPSYEDKFARKGIRVVDLLNPEVLQEKLRDNLAAKNK
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHH
LLKTIYEKEEFDIDAMVKEYAEFDKIIDPYVTNTQLYLNRQLKAGKTVLLEGAQGCLLDV
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCEEEC
DHGTYPYVTSSNPTSGGACTGSGIAPNYIGKVIGICKAYMTRVGNGAFPSELSDETGERL
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
GQIGHEFGATTGRKRRCGWIDLVALRYSLAVNGVTEIALTKLDVLDTFEEISVCTSYMLD
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKEIQDFPTDHQTLSRVTPVFKTMKGWKATNADARTFDEMRPEARNYVTFLEEELQVPVT
CCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHCCCEE
FISVGPGRDETVFR
EEEECCCCCCCCCC
>Mature Secondary Structure
MESPSFSKPVQSATVIVGTQFGDEGKGKLVDYLSDKYDIVVRYQGGANAGHTICFDNKTI
CCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCEEEECCCEE
VLHLLPSGIFHEGCVCVIGNGVVIDPAALLDEIRKVEELGYEVKGRLFISHNAHLIMPYH
EEEECCCCCCCCCCEEEECCCEEECHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEHHH
KRLDSLHEDAQGDQKIGTTGRGIGPSYEDKFARKGIRVVDLLNPEVLQEKLRDNLAAKNK
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHH
LLKTIYEKEEFDIDAMVKEYAEFDKIIDPYVTNTQLYLNRQLKAGKTVLLEGAQGCLLDV
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCEEEC
DHGTYPYVTSSNPTSGGACTGSGIAPNYIGKVIGICKAYMTRVGNGAFPSELSDETGERL
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
GQIGHEFGATTGRKRRCGWIDLVALRYSLAVNGVTEIALTKLDVLDTFEEISVCTSYMLD
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKEIQDFPTDHQTLSRVTPVFKTMKGWKATNADARTFDEMRPEARNYVTFLEEELQVPVT
CCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHCCCEE
FISVGPGRDETVFR
EEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA