Click here to switch to the map view.

The map label for this gene is dus1 [H]

Identifier: 119358060

GI number: 119358060

Start: 2619183

End: 2620226

Strand: Reverse

Name: dus1 [H]

Synonym: Cpha266_2288

Alternate gene names: 119358060

Gene position: 2620226-2619183 (Counterclockwise)

Preceding gene: 119358061

Following gene: 119358059

Centisome position: 83.61

GC content: 49.62

Gene sequence:

>1044_bases
ATGAGAATCGGTCCTATTGATATTGATCGTCCCGTTATTCTTGCTCCGATGGAGGATGTGACCGACAGGGCTTTTCGGCA
GCTTTGCAAGCGGTTCGGCGCTGATATCGTCTATACGGAATTTGTCAGTGCCGAAGCCTTGCGCAGGGGTGCAGAAAAAA
CGCTTCGCAAGCTTGTTCTTGAAGCTGGAGAGCGTCCCGTTGCCGTGCAGATTTTTGGCAGTACCGTTGAGTCGATGGTT
GAGGCGACAGCGGTTGCCGAGGAGTATGCTCCGGATTTTATTGATATCAACTTCGGCTGTCCCACAAAAAAAGTGGCAGG
CAAAGGAGCTGGTGCGGCTCTGCTCAGGGAACCAGGGAAAATGGCCGCAATCGCCGAAGCGGTGGTTCGTCGTTCCAGTC
TGCCGGTAACGGCAAAAACAAGAATCGGCTGGGACCATGATTCGATCAATATTATTGATGTACTGCGCCGGCTTGAGGAT
GCAGGAATTCAGGCTCTTGCCATACATGGCCGTACCAGGAGCGACATGTACAAGGGTCGTGCCGACTGGAACTGGATAGC
CGAGGCAAAAAACCATGCAAGAATACCTGTTATCGCCAACGGTGATATCTGGTCGGCAGAGGATGCCCTTGCCATGTTTC
GCCATACCGGGGCTGATGGAATCATGATCGGCAGGGGAGCGATCGGCAATCCGTTTATTTTCGGTCAGGCAAAACATCTT
CTTCAAACGGGAGTCGTTTTGCCCATGCCAGGTTTCAGCCTGAGAATTCATGCGGCCATCGATCATCTTGAACTTTCGGT
TGCCTGTAAAGGAGAGAAATACGGAACGATTGAAATGCGCCGTCATTACTCCACCTATCTGAAAGGTCTTCCAAAAGTAT
CCGTTGTTCGTAACGCGATTGTTCGTGAAGATGACTGGCAGCTTGTTACAGAAATTTTACTCGCTTACGAGCGTGAGTGT
GAAGGGTATGCAAGAGATGGACGTATAAAGGAGTATGCGGAATATCTCAATGATCATTCAGATAAACTTGTTTTGAATTA
TTAA

Upstream 100 bases:

>100_bases
TTAAAAAAGCGCTACGGGAGGATCATGCGCTCTTCCAGCAGATTTATATGCAGGTCAAGGAGTTTATGACCGGAAAAGTT
GAAATTATCAGCGGCGGAAA

Downstream 100 bases:

>100_bases
GAAACCATAACGTATGAGTAATCCGGAAACCCGCTGTCGTGTAGCGGTGCTTGGTGCAACCGGCCTTGTCGGCCGAACCA
TGATTCAGGTTCTTGAAGAG

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MRIGPIDIDRPVILAPMEDVTDRAFRQLCKRFGADIVYTEFVSAEALRRGAEKTLRKLVLEAGERPVAVQIFGSTVESMV
EATAVAEEYAPDFIDINFGCPTKKVAGKGAGAALLREPGKMAAIAEAVVRRSSLPVTAKTRIGWDHDSINIIDVLRRLED
AGIQALAIHGRTRSDMYKGRADWNWIAEAKNHARIPVIANGDIWSAEDALAMFRHTGADGIMIGRGAIGNPFIFGQAKHL
LQTGVVLPMPGFSLRIHAAIDHLELSVACKGEKYGTIEMRRHYSTYLKGLPKVSVVRNAIVREDDWQLVTEILLAYEREC
EGYARDGRIKEYAEYLNDHSDKLVLNY

Sequences:

>Translated_347_residues
MRIGPIDIDRPVILAPMEDVTDRAFRQLCKRFGADIVYTEFVSAEALRRGAEKTLRKLVLEAGERPVAVQIFGSTVESMV
EATAVAEEYAPDFIDINFGCPTKKVAGKGAGAALLREPGKMAAIAEAVVRRSSLPVTAKTRIGWDHDSINIIDVLRRLED
AGIQALAIHGRTRSDMYKGRADWNWIAEAKNHARIPVIANGDIWSAEDALAMFRHTGADGIMIGRGAIGNPFIFGQAKHL
LQTGVVLPMPGFSLRIHAAIDHLELSVACKGEKYGTIEMRRHYSTYLKGLPKVSVVRNAIVREDDWQLVTEILLAYEREC
EGYARDGRIKEYAEYLNDHSDKLVLNY
>Mature_347_residues
MRIGPIDIDRPVILAPMEDVTDRAFRQLCKRFGADIVYTEFVSAEALRRGAEKTLRKLVLEAGERPVAVQIFGSTVESMV
EATAVAEEYAPDFIDINFGCPTKKVAGKGAGAALLREPGKMAAIAEAVVRRSSLPVTAKTRIGWDHDSINIIDVLRRLED
AGIQALAIHGRTRSDMYKGRADWNWIAEAKNHARIPVIANGDIWSAEDALAMFRHTGADGIMIGRGAIGNPFIFGQAKHL
LQTGVVLPMPGFSLRIHAAIDHLELSVACKGEKYGTIEMRRHYSTYLKGLPKVSVVRNAIVREDDWQLVTEILLAYEREC
EGYARDGRIKEYAEYLNDHSDKLVLNY

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=238, Percent_Identity=35.2941176470588, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI239788462, Length=237, Percent_Identity=34.5991561181435, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=33.4782608695652, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=30.3964757709251, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI8923374, Length=241, Percent_Identity=31.9502074688797, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789660, Length=290, Percent_Identity=39.6551724137931, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788462, Length=232, Percent_Identity=35.3448275862069, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI145693211, Length=234, Percent_Identity=30.7692307692308, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=237, Percent_Identity=32.9113924050633, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17543114, Length=236, Percent_Identity=30.0847457627119, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144369, Length=219, Percent_Identity=31.9634703196347, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17510279, Length=244, Percent_Identity=29.5081967213115, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323437, Length=233, Percent_Identity=31.7596566523605, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323560, Length=306, Percent_Identity=28.7581699346405, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323433, Length=314, Percent_Identity=24.203821656051, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24580595, Length=258, Percent_Identity=31.3953488372093, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19920448, Length=258, Percent_Identity=31.3953488372093, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24585320, Length=243, Percent_Identity=35.3909465020576, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=29.2576419213974, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI45549423, Length=260, Percent_Identity=31.5384615384615, Blast_Score=88, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38516; Mature: 38516

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGPIDIDRPVILAPMEDVTDRAFRQLCKRFGADIVYTEFVSAEALRRGAEKTLRKLVL
CCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHH
EAGERPVAVQIFGSTVESMVEATAVAEEYAPDFIDINFGCPTKKVAGKGAGAALLREPGK
HCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHCCCCCCHHHHCCCCH
MAAIAEAVVRRSSLPVTAKTRIGWDHDSINIIDVLRRLEDAGIQALAIHGRTRSDMYKGR
HHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHCCC
ADWNWIAEAKNHARIPVIANGDIWSAEDALAMFRHTGADGIMIGRGAIGNPFIFGQAKHL
CCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCEEEHHHHHH
LQTGVVLPMPGFSLRIHAAIDHLELSVACKGEKYGTIEMRRHYSTYLKGLPKVSVVRNAI
HHCCCEEECCCCCEEEEEEECEEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
VREDDWQLVTEILLAYERECEGYARDGRIKEYAEYLNDHSDKLVLNY
HCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MRIGPIDIDRPVILAPMEDVTDRAFRQLCKRFGADIVYTEFVSAEALRRGAEKTLRKLVL
CCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHH
EAGERPVAVQIFGSTVESMVEATAVAEEYAPDFIDINFGCPTKKVAGKGAGAALLREPGK
HCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHCCCCCCHHHHCCCCH
MAAIAEAVVRRSSLPVTAKTRIGWDHDSINIIDVLRRLEDAGIQALAIHGRTRSDMYKGR
HHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHCCC
ADWNWIAEAKNHARIPVIANGDIWSAEDALAMFRHTGADGIMIGRGAIGNPFIFGQAKHL
CCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCEEEHHHHHH
LQTGVVLPMPGFSLRIHAAIDHLELSVACKGEKYGTIEMRRHYSTYLKGLPKVSVVRNAI
HHCCCEEECCCCCEEEEEEECEEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
VREDDWQLVTEILLAYERECEGYARDGRIKEYAEYLNDHSDKLVLNY
HCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]