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The map label for this gene is menF [H]

Identifier: 119357880

GI number: 119357880

Start: 2420529

End: 2421962

Strand: Direct

Name: menF [H]

Synonym: Cpha266_2088

Alternate gene names: 119357880

Gene position: 2420529-2421962 (Clockwise)

Preceding gene: 119357879

Following gene: 119357881

Centisome position: 77.24

GC content: 52.09

Gene sequence:

>1434_bases
ATGAGTGACAAGCGACATACCATTATTGCTGACAACAACCCGCTTCCTCTGCAAAAGGCCGTTCAAAGCCTTCTGCGCGA
GGTTGAACGGTTAAAAGAGAGAACGACAGATCGATTCGAAGAAAGCCCGTCCGGCTCGCTTCTGACCATCAGCCAGCCTC
TTCTTCCGCTCGATCCGCTTGACTGGCTTAACCGCCAGCATCTCTTCCCCAAACTGTACTGGATGAACCGCGAAAAAAGT
TTTTCGGTCGCAGGAATAGGTACGGCTGATTGCATTGAACAAAACACGCCGGGCACAAACGCATCGAGTTTTGCCGAGCT
TACCCGGACAATAGCCACAAAAGATCCCGACGCGCGGTATTTCGGAGCATTCCGGTTCAACAATATGGAGGAACAAAGCG
AGCCGTGGCACTCATTCTCCTCCTATGCTTTTGTTCTGCCCCTTGTCGGGATAACGTTTGAACAGGAACGGTACGTACTC
TTCTGCAATCTTTGGCTGGAACCGGGGGAGGCGCCTGACATAAAAATCCGGAGCATTTGTGATGCTCTCGAAAACATGTC
GACCACGCAGTCGGATTGCGATAGCGACCGAAATATTCCCGCACTGGTGCGTATCTCCCGCAATCCGGATGTACAAAGCT
GGACCAGACAGTGCGAACGGGCACTGCGAACATTCGAGGCAGGCGACATGGATAAAATCATGCTGGCCCGACAAACCATT
CTTGAATTTTCGGAAAGTTTTTCGCCGCTGCTTTTTCTCATCAACTATCCTTATCCGAAAAACTCGACCTACCGGTTTTA
CTTTGAGCCGAAAAAAAACCATGCGTTTTTCAGTTTCACTCCTGAACGCCTCTATCGCAGGGATGGCGTCACGTTGCAGA
CCGAAGCCCTTGCGGGAACCAGTCTGAAAGAGAATCTCACCGGTGACGACAACCTTGCTTCCGAAGTCCTTCTGAACTCC
GAAAAAGATATCAGGGAACACAAATTCGTCAAAGACAGCATCTACGGGGAGCTGTTTCCGGTTTGCAGCGAGATTCAGAT
GGATGAACAGGTCCATGTGCTTCAACTGAACCGTCTGGCTCATCTTTATACCCGATGCAGCGCAACGCTCAAGCCGGAGT
TCAGCAATGACAGTACCTTGCTCACCCGCCTCCACCCTACGCCTGCCGTTGGAGGAGTTCCGAGGGATGAGGCGCTTCGG
CATATTCTCGATATTGAACCCTTCAACCGCGGGTGGTATGCCGGCCCTGCCGGATGGATAAGCAGCAATGCCGCTGAATT
CTGTGTCGGCATCCGATCCGGAGTCGTTGTCGAAGCGATGACCTTTCTCTACTCCGGTGCCGGTCTGGTCAAGGGATCAG
ACCCCGTCTCGGAATGGGATGAAATCGAGCAGAAAATCGGAGATCTCCTGACCACAGCAAACGGCGATACATGA

Upstream 100 bases:

>100_bases
TTACTGCGCCGGCTGAAAGTAAACGTTTCTGTGGACATCATGGACCATGAAAAACAAGCAGAACCGTCATGTCCGGGAGT
TATAATGCCGATAAACGCAT

Downstream 100 bases:

>100_bases
ACAATCGTGAAATAACATCACTGTGGAGCCGACTCATTGTCGAAGAACTCATCCGGCAGGGCTGCGGATTCTTCTGTCTC
TCTCCCGGCTCCCGTTCTAC

Product: isochorismate synthases

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSDKRHTIIADNNPLPLQKAVQSLLREVERLKERTTDRFEESPSGSLLTISQPLLPLDPLDWLNRQHLFPKLYWMNREKS
FSVAGIGTADCIEQNTPGTNASSFAELTRTIATKDPDARYFGAFRFNNMEEQSEPWHSFSSYAFVLPLVGITFEQERYVL
FCNLWLEPGEAPDIKIRSICDALENMSTTQSDCDSDRNIPALVRISRNPDVQSWTRQCERALRTFEAGDMDKIMLARQTI
LEFSESFSPLLFLINYPYPKNSTYRFYFEPKKNHAFFSFTPERLYRRDGVTLQTEALAGTSLKENLTGDDNLASEVLLNS
EKDIREHKFVKDSIYGELFPVCSEIQMDEQVHVLQLNRLAHLYTRCSATLKPEFSNDSTLLTRLHPTPAVGGVPRDEALR
HILDIEPFNRGWYAGPAGWISSNAAEFCVGIRSGVVVEAMTFLYSGAGLVKGSDPVSEWDEIEQKIGDLLTTANGDT

Sequences:

>Translated_477_residues
MSDKRHTIIADNNPLPLQKAVQSLLREVERLKERTTDRFEESPSGSLLTISQPLLPLDPLDWLNRQHLFPKLYWMNREKS
FSVAGIGTADCIEQNTPGTNASSFAELTRTIATKDPDARYFGAFRFNNMEEQSEPWHSFSSYAFVLPLVGITFEQERYVL
FCNLWLEPGEAPDIKIRSICDALENMSTTQSDCDSDRNIPALVRISRNPDVQSWTRQCERALRTFEAGDMDKIMLARQTI
LEFSESFSPLLFLINYPYPKNSTYRFYFEPKKNHAFFSFTPERLYRRDGVTLQTEALAGTSLKENLTGDDNLASEVLLNS
EKDIREHKFVKDSIYGELFPVCSEIQMDEQVHVLQLNRLAHLYTRCSATLKPEFSNDSTLLTRLHPTPAVGGVPRDEALR
HILDIEPFNRGWYAGPAGWISSNAAEFCVGIRSGVVVEAMTFLYSGAGLVKGSDPVSEWDEIEQKIGDLLTTANGDT
>Mature_476_residues
SDKRHTIIADNNPLPLQKAVQSLLREVERLKERTTDRFEESPSGSLLTISQPLLPLDPLDWLNRQHLFPKLYWMNREKSF
SVAGIGTADCIEQNTPGTNASSFAELTRTIATKDPDARYFGAFRFNNMEEQSEPWHSFSSYAFVLPLVGITFEQERYVLF
CNLWLEPGEAPDIKIRSICDALENMSTTQSDCDSDRNIPALVRISRNPDVQSWTRQCERALRTFEAGDMDKIMLARQTIL
EFSESFSPLLFLINYPYPKNSTYRFYFEPKKNHAFFSFTPERLYRRDGVTLQTEALAGTSLKENLTGDDNLASEVLLNSE
KDIREHKFVKDSIYGELFPVCSEIQMDEQVHVLQLNRLAHLYTRCSATLKPEFSNDSTLLTRLHPTPAVGGVPRDEALRH
ILDIEPFNRGWYAGPAGWISSNAAEFCVGIRSGVVVEAMTFLYSGAGLVKGSDPVSEWDEIEQKIGDLLTTANGDT

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=462, Percent_Identity=30.7359307359307, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1786809, Length=263, Percent_Identity=28.5171102661597, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788114, Length=204, Percent_Identity=26.4705882352941, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=210, Percent_Identity=26.1904761904762, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 54123; Mature: 53991

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKRHTIIADNNPLPLQKAVQSLLREVERLKERTTDRFEESPSGSLLTISQPLLPLDPL
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCH
DWLNRQHLFPKLYWMNREKSFSVAGIGTADCIEQNTPGTNASSFAELTRTIATKDPDARY
HHHCHHHCCCEEEEECCCCCEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCE
FGAFRFNNMEEQSEPWHSFSSYAFVLPLVGITFEQERYVLFCNLWLEPGEAPDIKIRSIC
EEEEEECCCCHHCCCHHHHHHHHHEEHHHCEEECCCCEEEEEEEEECCCCCCCCHHHHHH
DALENMSTTQSDCDSDRNIPALVRISRNPDVQSWTRQCERALRTFEAGDMDKIMLARQTI
HHHHHCCCCHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LEFSESFSPLLFLINYPYPKNSTYRFYFEPKKNHAFFSFTPERLYRRDGVTLQTEALAGT
HHHHHCCCCEEEEEECCCCCCCEEEEEEECCCCCEEEEECHHHHHHHCCCEEEEHHHCCC
SLKENLTGDDNLASEVLLNSEKDIREHKFVKDSIYGELFPVCSEIQMDEQVHVLQLNRLA
CHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
HLYTRCSATLKPEFSNDSTLLTRLHPTPAVGGVPRDEALRHILDIEPFNRGWYAGPAGWI
HHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHH
SSNAAEFCVGIRSGVVVEAMTFLYSGAGLVKGSDPVSEWDEIEQKIGDLLTTANGDT
CCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SDKRHTIIADNNPLPLQKAVQSLLREVERLKERTTDRFEESPSGSLLTISQPLLPLDPL
CCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCH
DWLNRQHLFPKLYWMNREKSFSVAGIGTADCIEQNTPGTNASSFAELTRTIATKDPDARY
HHHCHHHCCCEEEEECCCCCEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCE
FGAFRFNNMEEQSEPWHSFSSYAFVLPLVGITFEQERYVLFCNLWLEPGEAPDIKIRSIC
EEEEEECCCCHHCCCHHHHHHHHHEEHHHCEEECCCCEEEEEEEEECCCCCCCCHHHHHH
DALENMSTTQSDCDSDRNIPALVRISRNPDVQSWTRQCERALRTFEAGDMDKIMLARQTI
HHHHHCCCCHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LEFSESFSPLLFLINYPYPKNSTYRFYFEPKKNHAFFSFTPERLYRRDGVTLQTEALAGT
HHHHHCCCCEEEEEECCCCCCCEEEEEEECCCCCEEEEECHHHHHHHCCCEEEEHHHCCC
SLKENLTGDDNLASEVLLNSEKDIREHKFVKDSIYGELFPVCSEIQMDEQVHVLQLNRLA
CHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
HLYTRCSATLKPEFSNDSTLLTRLHPTPAVGGVPRDEALRHILDIEPFNRGWYAGPAGWI
HHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHH
SSNAAEFCVGIRSGVVVEAMTFLYSGAGLVKGSDPVSEWDEIEQKIGDLLTTANGDT
CCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]