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The map label for this gene is menD

Identifier: 119357881

GI number: 119357881

Start: 2421959

End: 2423722

Strand: Direct

Name: menD

Synonym: Cpha266_2089

Alternate gene names: 119357881

Gene position: 2421959-2423722 (Clockwise)

Preceding gene: 119357880

Following gene: 119357882

Centisome position: 77.28

GC content: 54.31

Gene sequence:

>1764_bases
ATGAACAATCGTGAAATAACATCACTGTGGAGCCGACTCATTGTCGAAGAACTCATCCGGCAGGGCTGCGGATTCTTCTG
TCTCTCTCCCGGCTCCCGTTCTACCCCGCTTACGGTGGCCGTGGCACGAAATCCGATGGCAAAATGGAAAACATTTCCCG
ATGAACGTTCTGCGGGGTTTTTCGCACTTGGCCACGCCAGGGCAACCGGTAAACCGGCAATCCTCATCTGTACCTCCGGT
ACCGCCGTGGCAAACTATTTTCCGTCAGTGGTAGAGGCCTCGATGGACTGCCAGCCCATGATTATTCTTTCGGCTGACAG
ACCTTTTGAACTGCTCGAAACAGGCGCCAATCAAACGATACGCCAGTCGGGCATGTTCGGCTCATACAGCCGATGGAACT
TCCGGTTACCTGAACCCTCTGCGGAGATTCCTCTTCAGTCGGTACTCTCGGCCATCGATCATGCCGTCGAAAAAGCAACG
GGAAATCCGCCCGGCCCGGTTCATCTTAATGTGCCCTTCAGGGAGCCGTTCGAACCTGAACTTTCCGATCTTAAAGACCC
CTGGCTTGCGCCTGTAGCTCACTGGATCGACTCTGCGGAACCTCTTCGCCGCTGCAGTCATGCCGAGAACGTTCCTCATC
CTGCCGTTATCGAAAAGACAGCGGAACTCATCAAGGCATCCCGCCAGCCGCTCTTTATTGCGGGAAAACTTGATTCCCGG
GAAGACGCGATTGCCGTCGGGCTCCTTGCCGGTTCGCTCGGTATTCCGCTCTATGCGGATCTGTCGTCCGGGCTGCGTTA
CAGCACTCACTGCAAACCGTGGCAGCTTGCCTTTCAGTCGCCGGCGTTTCTTGAACACTACCGGCCTGATATGGTGGTTC
ATTTCGGCGGACAGTTGATATCCCGACACCCTGGCGCCGCCATCCGGGTCAGCCATCCCGGAAACTATCTTGTTGTGAAA
CCCTCTGCCAACCGTTACGCACCCGACCACAACGTGACGCTGAGCATCGAAGCGTCACCGAAACTCTTTGCTGACGCGCT
CCTGCGTGACCTGCAGAACGTGAGTTTGCCTGCATGGAAGGGGCCTGTTGCTGATGAGTTTTTCACCTTCTCAGAGGAGG
AAATTGAGCACTACTGCGAGGACGACAGGCCTGTTTCCGAGATTTCAGTCGCGCGGATTGTCTCTCGGCTTACGGGGGAG
CATGATGGCCTTTTTATGTCCAACAGCATGCCGGTGAGGGATATGGATCTCTTCGCTGCCGCCACACGCCAGACCCCGAC
GATCTCCATTGCCATGAACAGAGGCGCAAGCGGTATTGACGGCATTCTTTCAACAGCGGCTGGTTTTGCCGAAGGACTTC
AGAAACCGGTAACCCTTGTCATCGGCGATATTGCGTTTCTTCATGACCTCAACGCGCTCTCCCTGCTTGAAAGCAGCTCT
GTTCCTCTGCGGATTATAGTCGTGAACAATAACGGCGGCGGCATTTTTTCGTTTTTACCCATTGCTGCAGAGAAGGATGT
TTTTGAGACGCATTTTGCCACGCCGCAACATTACAGCATAGCCTCCGCTGCCGCAACCTTCGGGGTTGCCCATGCCTCGC
CGAAAACAAACCGGGAGTTTGCCGACACCTGTACAGAGGCATTTGAAAGCAATGAGAGTATCGTTATAGAGATTACGGGA
AGCCGTGAGGATAATGTCAGCGCACACCGCTCTTTGCAGCATACTATCAATATGCTGGCCCGAAAATTTCTGAAAAAACA
GTAA

Upstream 100 bases:

>100_bases
ACTCCGGTGCCGGTCTGGTCAAGGGATCAGACCCCGTCTCGGAATGGGATGAAATCGAGCAGAAAATCGGAGATCTCCTG
ACCACAGCAAACGGCGATAC

Downstream 100 bases:

>100_bases
CGGCAAGCCTTTTCCCCGATCACCTTTTCCGTTTTGTCGTTTCCGCAAGGCGATTCGGCAATCCCGTTCATAATGCCGGA
ACCCCAACAGAGCAATCTCT

Product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MNNREITSLWSRLIVEELIRQGCGFFCLSPGSRSTPLTVAVARNPMAKWKTFPDERSAGFFALGHARATGKPAILICTSG
TAVANYFPSVVEASMDCQPMIILSADRPFELLETGANQTIRQSGMFGSYSRWNFRLPEPSAEIPLQSVLSAIDHAVEKAT
GNPPGPVHLNVPFREPFEPELSDLKDPWLAPVAHWIDSAEPLRRCSHAENVPHPAVIEKTAELIKASRQPLFIAGKLDSR
EDAIAVGLLAGSLGIPLYADLSSGLRYSTHCKPWQLAFQSPAFLEHYRPDMVVHFGGQLISRHPGAAIRVSHPGNYLVVK
PSANRYAPDHNVTLSIEASPKLFADALLRDLQNVSLPAWKGPVADEFFTFSEEEIEHYCEDDRPVSEISVARIVSRLTGE
HDGLFMSNSMPVRDMDLFAAATRQTPTISIAMNRGASGIDGILSTAAGFAEGLQKPVTLVIGDIAFLHDLNALSLLESSS
VPLRIIVVNNNGGGIFSFLPIAAEKDVFETHFATPQHYSIASAAATFGVAHASPKTNREFADTCTEAFESNESIVIEITG
SREDNVSAHRSLQHTINMLARKFLKKQ

Sequences:

>Translated_587_residues
MNNREITSLWSRLIVEELIRQGCGFFCLSPGSRSTPLTVAVARNPMAKWKTFPDERSAGFFALGHARATGKPAILICTSG
TAVANYFPSVVEASMDCQPMIILSADRPFELLETGANQTIRQSGMFGSYSRWNFRLPEPSAEIPLQSVLSAIDHAVEKAT
GNPPGPVHLNVPFREPFEPELSDLKDPWLAPVAHWIDSAEPLRRCSHAENVPHPAVIEKTAELIKASRQPLFIAGKLDSR
EDAIAVGLLAGSLGIPLYADLSSGLRYSTHCKPWQLAFQSPAFLEHYRPDMVVHFGGQLISRHPGAAIRVSHPGNYLVVK
PSANRYAPDHNVTLSIEASPKLFADALLRDLQNVSLPAWKGPVADEFFTFSEEEIEHYCEDDRPVSEISVARIVSRLTGE
HDGLFMSNSMPVRDMDLFAAATRQTPTISIAMNRGASGIDGILSTAAGFAEGLQKPVTLVIGDIAFLHDLNALSLLESSS
VPLRIIVVNNNGGGIFSFLPIAAEKDVFETHFATPQHYSIASAAATFGVAHASPKTNREFADTCTEAFESNESIVIEITG
SREDNVSAHRSLQHTINMLARKFLKKQ
>Mature_587_residues
MNNREITSLWSRLIVEELIRQGCGFFCLSPGSRSTPLTVAVARNPMAKWKTFPDERSAGFFALGHARATGKPAILICTSG
TAVANYFPSVVEASMDCQPMIILSADRPFELLETGANQTIRQSGMFGSYSRWNFRLPEPSAEIPLQSVLSAIDHAVEKAT
GNPPGPVHLNVPFREPFEPELSDLKDPWLAPVAHWIDSAEPLRRCSHAENVPHPAVIEKTAELIKASRQPLFIAGKLDSR
EDAIAVGLLAGSLGIPLYADLSSGLRYSTHCKPWQLAFQSPAFLEHYRPDMVVHFGGQLISRHPGAAIRVSHPGNYLVVK
PSANRYAPDHNVTLSIEASPKLFADALLRDLQNVSLPAWKGPVADEFFTFSEEEIEHYCEDDRPVSEISVARIVSRLTGE
HDGLFMSNSMPVRDMDLFAAATRQTPTISIAMNRGASGIDGILSTAAGFAEGLQKPVTLVIGDIAFLHDLNALSLLESSS
VPLRIIVVNNNGGGIFSFLPIAAEKDVFETHFATPQHYSIASAAATFGVAHASPKTNREFADTCTEAFESNESIVIEITG
SREDNVSAHRSLQHTINMLARKFLKKQ

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=571, Percent_Identity=33.2749562171629, Blast_Score=282, Evalue=5e-77,
Organism=Escherichia coli, GI1790104, Length=162, Percent_Identity=27.1604938271605, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323755, Length=575, Percent_Identity=22.7826086956522, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_CHLPD (A1BI74)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_912525.1
- ProteinModelPortal:   A1BI74
- STRING:   A1BI74
- GeneID:   4570437
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_2089
- NMPDR:   fig|290317.7.peg.2207
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   CLSK637963
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 64082; Mature: 64082

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNREITSLWSRLIVEELIRQGCGFFCLSPGSRSTPLTVAVARNPMAKWKTFPDERSAGF
CCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCHHHHCCCCCCCCCCE
FALGHARATGKPAILICTSGTAVANYFPSVVEASMDCQPMIILSADRPFELLETGANQTI
EEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCHHH
RQSGMFGSYSRWNFRLPEPSAEIPLQSVLSAIDHAVEKATGNPPGPVHLNVPFREPFEPE
HHCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCC
LSDLKDPWLAPVAHWIDSAEPLRRCSHAENVPHPAVIEKTAELIKASRQPLFIAGKLDSR
HHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCC
EDAIAVGLLAGSLGIPLYADLSSGLRYSTHCKPWQLAFQSPAFLEHYRPDMVVHFGGQLI
CCCEEEEEECCCCCCCEEEEHHCCCEECCCCCCHHEECCCCHHHHHCCCCEEEEECCHHH
SRHPGAAIRVSHPGNYLVVKPSANRYAPDHNVTLSIEASPKLFADALLRDLQNVSLPAWK
HCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCC
GPVADEFFTFSEEEIEHYCEDDRPVSEISVARIVSRLTGEHDGLFMSNSMPVRDMDLFAA
CCCCHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHH
ATRQTPTISIAMNRGASGIDGILSTAAGFAEGLQKPVTLVIGDIAFLHDLNALSLLESSS
HCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCHHHHHHCCC
VPLRIIVVNNNGGGIFSFLPIAAEKDVFETHFATPQHYSIASAAATFGVAHASPKTNREF
CCEEEEEEECCCCCEEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHH
ADTCTEAFESNESIVIEITGSREDNVSAHRSLQHTINMLARKFLKKQ
HHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNREITSLWSRLIVEELIRQGCGFFCLSPGSRSTPLTVAVARNPMAKWKTFPDERSAGF
CCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCHHHHCCCCCCCCCCE
FALGHARATGKPAILICTSGTAVANYFPSVVEASMDCQPMIILSADRPFELLETGANQTI
EEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCHHH
RQSGMFGSYSRWNFRLPEPSAEIPLQSVLSAIDHAVEKATGNPPGPVHLNVPFREPFEPE
HHCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCC
LSDLKDPWLAPVAHWIDSAEPLRRCSHAENVPHPAVIEKTAELIKASRQPLFIAGKLDSR
HHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCC
EDAIAVGLLAGSLGIPLYADLSSGLRYSTHCKPWQLAFQSPAFLEHYRPDMVVHFGGQLI
CCCEEEEEECCCCCCCEEEEHHCCCEECCCCCCHHEECCCCHHHHHCCCCEEEEECCHHH
SRHPGAAIRVSHPGNYLVVKPSANRYAPDHNVTLSIEASPKLFADALLRDLQNVSLPAWK
HCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCC
GPVADEFFTFSEEEIEHYCEDDRPVSEISVARIVSRLTGEHDGLFMSNSMPVRDMDLFAA
CCCCHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHH
ATRQTPTISIAMNRGASGIDGILSTAAGFAEGLQKPVTLVIGDIAFLHDLNALSLLESSS
HCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCHHHHHHCCC
VPLRIIVVNNNGGGIFSFLPIAAEKDVFETHFATPQHYSIASAAATFGVAHASPKTNREF
CCEEEEEEECCCCCEEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHH
ADTCTEAFESNESIVIEITGSREDNVSAHRSLQHTINMLARKFLKKQ
HHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA