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The map label for this gene is relA [H]

Identifier: 119357815

GI number: 119357815

Start: 2334331

End: 2336532

Strand: Reverse

Name: relA [H]

Synonym: Cpha266_2023

Alternate gene names: 119357815

Gene position: 2336532-2334331 (Counterclockwise)

Preceding gene: 119357816

Following gene: 119357814

Centisome position: 74.56

GC content: 47.32

Gene sequence:

>2202_bases
ATGTTAGCGCAAATAGATAAAGAACACTATAGTAAATTGCATGAGATACTGCAGCTTTCACGCAAAAACCTCAAGAATTT
CGACGAGTCTCTGATTCAGCGTGCGTTTTTTATGTGTTACAGGGCACATGAAGGCGAAAAAAGGGCCTCAGGCGAACCGT
TTTTTTATCATCCCGTCGAAGTGGCTACCATTCTTCTCAATGAGTTGCCTCTTGATGGTGTGTCGGTAGCCGCAGCGCTG
CTTCATGATGTTATTGAGGATAGCGGGTATACGTATGACGATCTGGTGGCCGAACTTGGCGTCGAGGTTGCGGATATTGT
GGAGGGGTTGACCAAAATATCAGGGATCACGATCAATCGCGAAACCTCGCAGGCCGAGGGGTTTCGTAAGATGCTTCTCT
CGATGGTCAAGGATATCAGGGTTATACTGATCAAATTCTGCGACCGTCTGCACAATATGAGAACGCTTGAATCTCTGCCG
GAGCATCGCAGGCTGAAAATAGCGCTGGAAACGCGGGACATTTACGCTCCGCTGGCGCACCGCTTCGGACTTGGAAAAAT
GAAAGTCGAGTTTGAAAATCTCGCGCTCAAATATATTGATCCCGAAATGTTTGAGTTTCTGACGCAGAAGGTTCGATTAA
GCAGAGCCGAACGTATCAGTTATCTCAAAAAAATGATCTCTCCGATAAAGGAGGATCTTGAAAAGCAGGGGTTCAAGGTG
GAGGTCGAGGGCAGGGCAAAACATCTGTTTTCGATTTACAACAAGATGAGAAACAAAAACAAGAACTTTGAGGATATTCA
CGATCTCTACGGCATACGGGTTATTATCGACACGGAGAGGATTGCCGACTGTTTTGCCGTTTATGGGTTCATAACCCAGA
AATATCCTCCGATTCCCCAGCATTTCAAGGATTACATATCCATTCCGAAGCATAATGGATATCAGTCGCTCCACTCGGCC
ATTATCGGCCCGAGAGGCAACATGGTCGAACTGCAGATCAGAACGAAACGGATGCACGAGTTTGCCGAGCTTGGCGTTGC
GGCTCACTGGCGATACAAGGAGAAAATTTCCAGGGATGATGTAAATATCGATGTGTTTCTTCGCTGGGCAAGGGAGCTGA
TAAAGGATGCCGACAGCGCAGCGGCTTTCATGGAAGGTTTTAAGCTGAATCTCTACCATGACGAGATCTATGTTTTTACC
CCGAAAGGCGATATGAAAACCATGCCTGCGGGCGCCACGCCGATCGATTTTGCCTATGCAATCCATACTGAAGTCGGTAA
CGGGTGTATCGGGGCGAAGGTAAATGGTAAAATAGTACGTCTGAACGCCGAGCTCAAGTCCGGCGACAGGCTTGAAATTA
TTACTTCGAAAAATCAGAAGCCGAGACAAGACTGGCTCAATAGTGTTGTAACGCACAGGGCAAAACTGAAGATACGTTCG
TCAATCAACGAAGAGCGTCGCCTGCAGATTGAAAAAGGTCGGGGGATGTGGGAAAAAATGCTCTCTGGTACGAAAAAGCT
GTTGACGGACAATGATATCATCCGGCAAGTCAAGCGCTACGGGATTAAAACCCCGGCTGATTTTTTCAGCGCTCTGGCTA
CCCAGGAGATAAGCGGCGAAGAGGTTATCGAACGGGTCACGAACGCAAAAAAAGAGGTCGCAGACTCTCCGGCAGTTACG
CTTAAGGATGATGTCCGCTCTGTTGATGGTTATCTTCGCAATGCCCGCCATGAGCAGGAGCATCAGGTTGCGAAAAAAGA
TGAGGTGATCATTGCCGGTATGAATAATATCGCCTATTCCTTTGCGAAATGCTGTCAGCCCGTTCCCGGTGACGAGGTTA
TCGGTTTTGTTACGATGGAAGGGATGGTGAAAATTCACCGGAAGAACTGTATCAATGTCAGTAATGAAAACCTCCTGAAA
AATGAGCGGGCGGTTTCGGTTACATGGAACAAAAAGGTTGAAACCGATTTTCTTGCCGGGATTCGGATTGTCGGGGAGGA
TCGTATTGGTATCACCAACCAGATCACCACGGTTATCTCGAAATCCGAGACGAATATTCGCGGTATTTCCCTGGATGCCA
AAGACGGAATGTTTACCGGAACGTTTATGGTTTATGTTCGTAATATTGAGAAGCTCACCTCGCTTATGGACAGGTTGAAA
AAGGTACAGGGAATTTTTACCGTTGAGCGGTTGCTGGGTTGA

Upstream 100 bases:

>100_bases
TTTCGCGACGGGCGGTTTTGTTATGCAGAAATAAAATTATATAAAGAAAGAGAGTGTATTCATACGTCACAACCATGTAG
TTGAGGGAGTTTGCAAGGTT

Downstream 100 bases:

>100_bases
TTTGTCTTTTTGGTATATGGGGTTATTATTATGTAGTTATAAACATGCAAAGCTGTTAACCAATAGAGGAGAAGTTTTAT
GAAAACCGGTTTTTTTCGAT

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 733; Mature: 733

Protein sequence:

>733_residues
MLAQIDKEHYSKLHEILQLSRKNLKNFDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVEVATILLNELPLDGVSVAAAL
LHDVIEDSGYTYDDLVAELGVEVADIVEGLTKISGITINRETSQAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLESLP
EHRRLKIALETRDIYAPLAHRFGLGKMKVEFENLALKYIDPEMFEFLTQKVRLSRAERISYLKKMISPIKEDLEKQGFKV
EVEGRAKHLFSIYNKMRNKNKNFEDIHDLYGIRVIIDTERIADCFAVYGFITQKYPPIPQHFKDYISIPKHNGYQSLHSA
IIGPRGNMVELQIRTKRMHEFAELGVAAHWRYKEKISRDDVNIDVFLRWARELIKDADSAAAFMEGFKLNLYHDEIYVFT
PKGDMKTMPAGATPIDFAYAIHTEVGNGCIGAKVNGKIVRLNAELKSGDRLEIITSKNQKPRQDWLNSVVTHRAKLKIRS
SINEERRLQIEKGRGMWEKMLSGTKKLLTDNDIIRQVKRYGIKTPADFFSALATQEISGEEVIERVTNAKKEVADSPAVT
LKDDVRSVDGYLRNARHEQEHQVAKKDEVIIAGMNNIAYSFAKCCQPVPGDEVIGFVTMEGMVKIHRKNCINVSNENLLK
NERAVSVTWNKKVETDFLAGIRIVGEDRIGITNQITTVISKSETNIRGISLDAKDGMFTGTFMVYVRNIEKLTSLMDRLK
KVQGIFTVERLLG

Sequences:

>Translated_733_residues
MLAQIDKEHYSKLHEILQLSRKNLKNFDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVEVATILLNELPLDGVSVAAAL
LHDVIEDSGYTYDDLVAELGVEVADIVEGLTKISGITINRETSQAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLESLP
EHRRLKIALETRDIYAPLAHRFGLGKMKVEFENLALKYIDPEMFEFLTQKVRLSRAERISYLKKMISPIKEDLEKQGFKV
EVEGRAKHLFSIYNKMRNKNKNFEDIHDLYGIRVIIDTERIADCFAVYGFITQKYPPIPQHFKDYISIPKHNGYQSLHSA
IIGPRGNMVELQIRTKRMHEFAELGVAAHWRYKEKISRDDVNIDVFLRWARELIKDADSAAAFMEGFKLNLYHDEIYVFT
PKGDMKTMPAGATPIDFAYAIHTEVGNGCIGAKVNGKIVRLNAELKSGDRLEIITSKNQKPRQDWLNSVVTHRAKLKIRS
SINEERRLQIEKGRGMWEKMLSGTKKLLTDNDIIRQVKRYGIKTPADFFSALATQEISGEEVIERVTNAKKEVADSPAVT
LKDDVRSVDGYLRNARHEQEHQVAKKDEVIIAGMNNIAYSFAKCCQPVPGDEVIGFVTMEGMVKIHRKNCINVSNENLLK
NERAVSVTWNKKVETDFLAGIRIVGEDRIGITNQITTVISKSETNIRGISLDAKDGMFTGTFMVYVRNIEKLTSLMDRLK
KVQGIFTVERLLG
>Mature_733_residues
MLAQIDKEHYSKLHEILQLSRKNLKNFDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVEVATILLNELPLDGVSVAAAL
LHDVIEDSGYTYDDLVAELGVEVADIVEGLTKISGITINRETSQAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLESLP
EHRRLKIALETRDIYAPLAHRFGLGKMKVEFENLALKYIDPEMFEFLTQKVRLSRAERISYLKKMISPIKEDLEKQGFKV
EVEGRAKHLFSIYNKMRNKNKNFEDIHDLYGIRVIIDTERIADCFAVYGFITQKYPPIPQHFKDYISIPKHNGYQSLHSA
IIGPRGNMVELQIRTKRMHEFAELGVAAHWRYKEKISRDDVNIDVFLRWARELIKDADSAAAFMEGFKLNLYHDEIYVFT
PKGDMKTMPAGATPIDFAYAIHTEVGNGCIGAKVNGKIVRLNAELKSGDRLEIITSKNQKPRQDWLNSVVTHRAKLKIRS
SINEERRLQIEKGRGMWEKMLSGTKKLLTDNDIIRQVKRYGIKTPADFFSALATQEISGEEVIERVTNAKKEVADSPAVT
LKDDVRSVDGYLRNARHEQEHQVAKKDEVIIAGMNNIAYSFAKCCQPVPGDEVIGFVTMEGMVKIHRKNCINVSNENLLK
NERAVSVTWNKKVETDFLAGIRIVGEDRIGITNQITTVISKSETNIRGISLDAKDGMFTGTFMVYVRNIEKLTSLMDRLK
KVQGIFTVERLLG

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=707, Percent_Identity=37.1994342291372, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1789147, Length=719, Percent_Identity=31.432545201669, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 83807; Mature: 83807

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAQIDKEHYSKLHEILQLSRKNLKNFDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHH
VATILLNELPLDGVSVAAALLHDVIEDSGYTYDDLVAELGVEVADIVEGLTKISGITINR
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEC
ETSQAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLESLPEHRRLKIALETRDIYAPLAH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHH
RFGLGKMKVEFENLALKYIDPEMFEFLTQKVRLSRAERISYLKKMISPIKEDLEKQGFKV
HCCCCCEEEEHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
EVEGRAKHLFSIYNKMRNKNKNFEDIHDLYGIRVIIDTERIADCFAVYGFITQKYPPIPQ
EECCHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHCCCCCCHH
HFKDYISIPKHNGYQSLHSAIIGPRGNMVELQIRTKRMHEFAELGVAAHWRYKEKISRDD
HHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCC
VNIDVFLRWARELIKDADSAAAFMEGFKLNLYHDEIYVFTPKGDMKTMPAGATPIDFAYA
CCHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEECEEEEECCCCCCCCCCCCCCCCHHHEE
IHTEVGNGCIGAKVNGKIVRLNAELKSGDRLEIITSKNQKPRQDWLNSVVTHRAKLKIRS
EEHHCCCCEEECEECCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
SINEERRLQIEKGRGMWEKMLSGTKKLLTDNDIIRQVKRYGIKTPADFFSALATQEISGE
HCCHHHCEEEHHCCCHHHHHHCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHH
EVIERVTNAKKEVADSPAVTLKDDVRSVDGYLRNARHEQEHQVAKKDEVIIAGMNNIAYS
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECHHHHHH
FAKCCQPVPGDEVIGFVTMEGMVKIHRKNCINVSNENLLKNERAVSVTWNKKVETDFLAG
HHHHCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCHHHCCCCEEEEEECCCCCHHHHHC
IRIVGEDRIGITNQITTVISKSETNIRGISLDAKDGMFTGTFMVYVRNIEKLTSLMDRLK
EEEEECCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
KVQGIFTVERLLG
HHHHHHHHHHHCC
>Mature Secondary Structure
MLAQIDKEHYSKLHEILQLSRKNLKNFDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHH
VATILLNELPLDGVSVAAALLHDVIEDSGYTYDDLVAELGVEVADIVEGLTKISGITINR
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEC
ETSQAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLESLPEHRRLKIALETRDIYAPLAH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHH
RFGLGKMKVEFENLALKYIDPEMFEFLTQKVRLSRAERISYLKKMISPIKEDLEKQGFKV
HCCCCCEEEEHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
EVEGRAKHLFSIYNKMRNKNKNFEDIHDLYGIRVIIDTERIADCFAVYGFITQKYPPIPQ
EECCHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHCCCCCCHH
HFKDYISIPKHNGYQSLHSAIIGPRGNMVELQIRTKRMHEFAELGVAAHWRYKEKISRDD
HHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCC
VNIDVFLRWARELIKDADSAAAFMEGFKLNLYHDEIYVFTPKGDMKTMPAGATPIDFAYA
CCHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEECEEEEECCCCCCCCCCCCCCCCHHHEE
IHTEVGNGCIGAKVNGKIVRLNAELKSGDRLEIITSKNQKPRQDWLNSVVTHRAKLKIRS
EEHHCCCCEEECEECCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
SINEERRLQIEKGRGMWEKMLSGTKKLLTDNDIIRQVKRYGIKTPADFFSALATQEISGE
HCCHHHCEEEHHCCCHHHHHHCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHH
EVIERVTNAKKEVADSPAVTLKDDVRSVDGYLRNARHEQEHQVAKKDEVIIAGMNNIAYS
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECHHHHHH
FAKCCQPVPGDEVIGFVTMEGMVKIHRKNCINVSNENLLKNERAVSVTWNKKVETDFLAG
HHHHCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCHHHCCCCEEEEEECCCCCHHHHHC
IRIVGEDRIGITNQITTVISKSETNIRGISLDAKDGMFTGTFMVYVRNIEKLTSLMDRLK
EEEEECCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
KVQGIFTVERLLG
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9383190; 9384377 [H]