Click here to switch to the map view.

The map label for this gene is rfbB [H]

Identifier: 119357788

GI number: 119357788

Start: 2310007

End: 2311080

Strand: Reverse

Name: rfbB [H]

Synonym: Cpha266_1995

Alternate gene names: 119357788

Gene position: 2311080-2310007 (Counterclockwise)

Preceding gene: 119357789

Following gene: 119357787

Centisome position: 73.74

GC content: 48.42

Gene sequence:

>1074_bases
ATGCATATACTTGTTACCGGGGGTGCAGGATTTATCGGTTCTCATGTCGTAAGGCATTTTGTGACAGGATATCCATCGTA
TACTATTACAAATCTTGACAGCCTGACCTATGCAGGCAACCTTGAAAATCTTGATGACATAAGGCATCTGTCGAACTACC
GGTTCGTCAAAGGCGACATTACCGACGCTGCCCTGATGAACTCTCTTTTTGAGGAGCAGCGATTTGACGGTGTTATCCAT
CTTGCTGCAGAATCTCACGTTGATCGTTCAATAGCAAGTCCTGCCGAGTTTGCCGTTACCAACGTTCTTGGTACCGTGCA
CCTTCTCAATGCCGCCCGACAAAGCTGGGCTGGGAATTATCAGGGCAAGCGGTTCTATCACATCTCAACGGATGAGGTCT
ATGGTGCGCTTGGCGGTGAGGGGATGTTTACCGAGGAGACTCCTTATGATCCTCACAGCCCCTATTCCGCATCGAAAGCT
TCGTCGGATCACTTTGTAAGGGCTTATAACGATACCTATGGCCTGCCCGTGGTCATCAGTAACTGTTCGAACAATTACGG
ACCATATCAGTTTCCTGAAAAACTCATTCCCTTGTTTATCAATAATATCCGCAATGGCAAGCCTCTGCCTGTTTATGGCA
AAGGGGAGAATATCCGGGACTGGTTATGGGTTGAAGATCACGCAAAAGCAATCGATGTGATCTATCATGGAGGAAAAGAT
GGTCAGACCTATAATATCGGAGGGAATAACGAGTGGAAAAATATTGATCTGGTCAGACAGCTTTGCGCAATTATGGACAG
CAAGCTCAATCGTCCTGCCGGAGAATCCGAAAAGCTGATTACCTATGTTACGGATCGTGCCGGTCACGATTTACGGTATG
CCATCGATTCGTCGAAGCTCCAGCGGGAGCTTGGATGGGCACCGTCCATACAGTTTGAAGAGGGTCTCGAACGAACGGTC
GACTGGTATCTTGCCAATCAGCACTGGCTCGATCAGGTTACTTCCGGCGCGTATCAGTCCTATTATGATGCGATGTACGG
AACGGGCGGAACTTCATCGGTGGTTTCCCGATGA

Upstream 100 bases:

>100_bases
CCTTACTGGAGAGACTCTCTGGAATCCGTGCTTCTTGCAGAAGAAGATGCCGGGCTATAAATTAGGAGACAGGTCATTAA
CTTATTGAAAAAGCAGATTC

Downstream 100 bases:

>100_bases
GCGGTGTTTTGCTCTATTGGTTGAACGAGGGTGATGAGAACGCGGTTTTGAGCTTGCCGCCTGTGTCGAAGTCCGGTGTA
CCGGTGCGACTTGTTTTTTT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MHILVTGGAGFIGSHVVRHFVTGYPSYTITNLDSLTYAGNLENLDDIRHLSNYRFVKGDITDAALMNSLFEEQRFDGVIH
LAAESHVDRSIASPAEFAVTNVLGTVHLLNAARQSWAGNYQGKRFYHISTDEVYGALGGEGMFTEETPYDPHSPYSASKA
SSDHFVRAYNDTYGLPVVISNCSNNYGPYQFPEKLIPLFINNIRNGKPLPVYGKGENIRDWLWVEDHAKAIDVIYHGGKD
GQTYNIGGNNEWKNIDLVRQLCAIMDSKLNRPAGESEKLITYVTDRAGHDLRYAIDSSKLQRELGWAPSIQFEEGLERTV
DWYLANQHWLDQVTSGAYQSYYDAMYGTGGTSSVVSR

Sequences:

>Translated_357_residues
MHILVTGGAGFIGSHVVRHFVTGYPSYTITNLDSLTYAGNLENLDDIRHLSNYRFVKGDITDAALMNSLFEEQRFDGVIH
LAAESHVDRSIASPAEFAVTNVLGTVHLLNAARQSWAGNYQGKRFYHISTDEVYGALGGEGMFTEETPYDPHSPYSASKA
SSDHFVRAYNDTYGLPVVISNCSNNYGPYQFPEKLIPLFINNIRNGKPLPVYGKGENIRDWLWVEDHAKAIDVIYHGGKD
GQTYNIGGNNEWKNIDLVRQLCAIMDSKLNRPAGESEKLITYVTDRAGHDLRYAIDSSKLQRELGWAPSIQFEEGLERTV
DWYLANQHWLDQVTSGAYQSYYDAMYGTGGTSSVVSR
>Mature_357_residues
MHILVTGGAGFIGSHVVRHFVTGYPSYTITNLDSLTYAGNLENLDDIRHLSNYRFVKGDITDAALMNSLFEEQRFDGVIH
LAAESHVDRSIASPAEFAVTNVLGTVHLLNAARQSWAGNYQGKRFYHISTDEVYGALGGEGMFTEETPYDPHSPYSASKA
SSDHFVRAYNDTYGLPVVISNCSNNYGPYQFPEKLIPLFINNIRNGKPLPVYGKGENIRDWLWVEDHAKAIDVIYHGGKD
GQTYNIGGNNEWKNIDLVRQLCAIMDSKLNRPAGESEKLITYVTDRAGHDLRYAIDSSKLQRELGWAPSIQFEEGLERTV
DWYLANQHWLDQVTSGAYQSYYDAMYGTGGTSSVVSR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=327, Percent_Identity=40.6727828746177, Blast_Score=262, Evalue=3e-70,
Organism=Homo sapiens, GI42516563, Length=328, Percent_Identity=23.780487804878, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI56237023, Length=355, Percent_Identity=26.1971830985915, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI56118217, Length=355, Percent_Identity=26.1971830985915, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI189083684, Length=355, Percent_Identity=26.1971830985915, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4504031, Length=237, Percent_Identity=28.2700421940928, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=55.1532033426184, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI48994969, Length=345, Percent_Identity=56.5217391304348, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1788366, Length=362, Percent_Identity=27.6243093922652, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786974, Length=350, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17568069, Length=331, Percent_Identity=35.9516616314199, Blast_Score=197, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI115532424, Length=323, Percent_Identity=33.4365325077399, Blast_Score=180, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI71982035, Length=362, Percent_Identity=29.8342541436464, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=364, Percent_Identity=29.6703296703297, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=330, Percent_Identity=24.8484848484848, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI133901788, Length=269, Percent_Identity=27.1375464684015, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=269, Percent_Identity=27.1375464684015, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=269, Percent_Identity=27.1375464684015, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=269, Percent_Identity=27.1375464684015, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=269, Percent_Identity=27.1375464684015, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=177, Percent_Identity=29.9435028248588, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=357, Percent_Identity=26.6106442577031, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356223, Length=329, Percent_Identity=24.6200607902736, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19923002, Length=356, Percent_Identity=27.247191011236, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39927; Mature: 39927

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHILVTGGAGFIGSHVVRHFVTGYPSYTITNLDSLTYAGNLENLDDIRHLSNYRFVKGDI
CEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCEEECCCCCCHHHHHHHHCCEEEECCC
TDAALMNSLFEEQRFDGVIHLAAESHVDRSIASPAEFAVTNVLGTVHLLNAARQSWAGNY
HHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
QGKRFYHISTDEVYGALGGEGMFTEETPYDPHSPYSASKASSDHFVRAYNDTYGLPVVIS
CCCEEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEE
NCSNNYGPYQFPEKLIPLFINNIRNGKPLPVYGKGENIRDWLWVEDHAKAIDVIYHGGKD
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEEECCCCCEEEEEEECCCC
GQTYNIGGNNEWKNIDLVRQLCAIMDSKLNRPAGESEKLITYVTDRAGHDLRYAIDSSKL
CCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEECCHHHH
QRELGWAPSIQFEEGLERTVDWYLANQHWLDQVTSGAYQSYYDAMYGTGGTSSVVSR
HHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MHILVTGGAGFIGSHVVRHFVTGYPSYTITNLDSLTYAGNLENLDDIRHLSNYRFVKGDI
CEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCEEECCCCCCHHHHHHHHCCEEEECCC
TDAALMNSLFEEQRFDGVIHLAAESHVDRSIASPAEFAVTNVLGTVHLLNAARQSWAGNY
HHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
QGKRFYHISTDEVYGALGGEGMFTEETPYDPHSPYSASKASSDHFVRAYNDTYGLPVVIS
CCCEEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEE
NCSNNYGPYQFPEKLIPLFINNIRNGKPLPVYGKGENIRDWLWVEDHAKAIDVIYHGGKD
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEEECCCCCEEEEEEECCCC
GQTYNIGGNNEWKNIDLVRQLCAIMDSKLNRPAGESEKLITYVTDRAGHDLRYAIDSSKL
CCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEECCHHHH
QRELGWAPSIQFEEGLERTVDWYLANQHWLDQVTSGAYQSYYDAMYGTGGTSSVVSR
HHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]