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The map label for this gene is spsK [H]

Identifier: 119357789

GI number: 119357789

Start: 2311121

End: 2311993

Strand: Reverse

Name: spsK [H]

Synonym: Cpha266_1996

Alternate gene names: 119357789

Gene position: 2311993-2311121 (Counterclockwise)

Preceding gene: 119357790

Following gene: 119357788

Centisome position: 73.77

GC content: 48.34

Gene sequence:

>873_bases
ATGAACATTCTTGTTACAGGCGGCAATGGACAGCTTGGTTCGGAGCTTCGTTCTCTTCAGCGGGACTATCCGGCATGGAT
GTTCTGTTTTTGCGATCTTCCTGACCTTGACATTACAGACAGTAACGCTGTCGATGCGTTGATGCGAACGTGCAAGTGTA
ATGTGATTGTTAATTGTGCGGCATATACAGCGGTTGACCAGGCAGAGCAGGAGCCAGGCAGAGCTTATCGGGTCAATCGT
GACGGGGCGGGCGTGCTTGCTGCTTGCGCGAAAGCTCATCATGCGCTGCTTATTCATATTTCAACAGATTATGTTTTTGA
CGGGAACTCCTGTCTTCCCTATCGCGAAACCGATCGGCCTGCCCCTCTTGGAATTTACGGGAAGTCGAAACGTGCTGCTG
AAGAGCTGATTTTTGAGATCGCTCCCTCTTTTCTGATTTTGCGTACCTCCTGGCTCTATTCGTTGTACGGGCATAATTTT
TTAAAAACCATGCTTCGGCTCGGGGCCGAAAAGGATGTGATCAAGGTTATTTTTGACCAGACAGGAACTCCGACTTATGC
GGGCGATCTTGCTCGCGCTATCATGACCATTCTTGGAAAGGTGCGATTGGATGAACAATACGGCGCGCTCTACCATTATT
CGAATGAAGGGGTATGTTCATGGTATGATTTTGCCGTTGCAATTATGGCTTTAAAGAAACTACCCTGCAAGGTGCTTCCG
ATAGAAACGACTGAATATCCGCTTCCTTCGCCGAGACCTTTTTTCAGCGTTCTCAATAAATCAGCAATAAAGCGGGACTG
GGGTCTGGTTATCCCTTACTGGAGAGACTCTCTGGAATCCGTGCTTCTTGCAGAAGAAGATGCCGGGCTATAA

Upstream 100 bases:

>100_bases
TTCCCTGCAGAAGTACAGGTACTCGTTTTATCGTTGACGGGTAATCGATTTTTTTTGAACGTTTTCAGCCACGGAACCCG
CTTATTATATTATTATTGTT

Downstream 100 bases:

>100_bases
ATTAGGAGACAGGTCATTAACTTATTGAAAAAGCAGATTCATGCATATACTTGTTACCGGGGGTGCAGGATTTATCGGTT
CTCATGTCGTAAGGCATTTT

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNILVTGGNGQLGSELRSLQRDYPAWMFCFCDLPDLDITDSNAVDALMRTCKCNVIVNCAAYTAVDQAEQEPGRAYRVNR
DGAGVLAACAKAHHALLIHISTDYVFDGNSCLPYRETDRPAPLGIYGKSKRAAEELIFEIAPSFLILRTSWLYSLYGHNF
LKTMLRLGAEKDVIKVIFDQTGTPTYAGDLARAIMTILGKVRLDEQYGALYHYSNEGVCSWYDFAVAIMALKKLPCKVLP
IETTEYPLPSPRPFFSVLNKSAIKRDWGLVIPYWRDSLESVLLAEEDAGL

Sequences:

>Translated_290_residues
MNILVTGGNGQLGSELRSLQRDYPAWMFCFCDLPDLDITDSNAVDALMRTCKCNVIVNCAAYTAVDQAEQEPGRAYRVNR
DGAGVLAACAKAHHALLIHISTDYVFDGNSCLPYRETDRPAPLGIYGKSKRAAEELIFEIAPSFLILRTSWLYSLYGHNF
LKTMLRLGAEKDVIKVIFDQTGTPTYAGDLARAIMTILGKVRLDEQYGALYHYSNEGVCSWYDFAVAIMALKKLPCKVLP
IETTEYPLPSPRPFFSVLNKSAIKRDWGLVIPYWRDSLESVLLAEEDAGL
>Mature_290_residues
MNILVTGGNGQLGSELRSLQRDYPAWMFCFCDLPDLDITDSNAVDALMRTCKCNVIVNCAAYTAVDQAEQEPGRAYRVNR
DGAGVLAACAKAHHALLIHISTDYVFDGNSCLPYRETDRPAPLGIYGKSKRAAEELIFEIAPSFLILRTSWLYSLYGHNF
LKTMLRLGAEKDVIKVIFDQTGTPTYAGDLARAIMTILGKVRLDEQYGALYHYSNEGVCSWYDFAVAIMALKKLPCKVLP
IETTEYPLPSPRPFFSVLNKSAIKRDWGLVIPYWRDSLESVLLAEEDAGL

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=285, Percent_Identity=31.5789473684211, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI33519455, Length=285, Percent_Identity=31.5789473684211, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1788352, Length=301, Percent_Identity=38.5382059800665, Blast_Score=181, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32336; Mature: 32336

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVTGGNGQLGSELRSLQRDYPAWMFCFCDLPDLDITDSNAVDALMRTCKCNVIVNCA
CEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEH
AYTAVDQAEQEPGRAYRVNRDGAGVLAACAKAHHALLIHISTDYVFDGNSCLPYRETDRP
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCCC
APLGIYGKSKRAAEELIFEIAPSFLILRTSWLYSLYGHNFLKTMLRLGAEKDVIKVIFDQ
CCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEECC
TGTPTYAGDLARAIMTILGKVRLDEQYGALYHYSNEGVCSWYDFAVAIMALKKLPCKVLP
CCCCCHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEE
IETTEYPLPSPRPFFSVLNKSAIKRDWGLVIPYWRDSLESVLLAEEDAGL
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNILVTGGNGQLGSELRSLQRDYPAWMFCFCDLPDLDITDSNAVDALMRTCKCNVIVNCA
CEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEH
AYTAVDQAEQEPGRAYRVNRDGAGVLAACAKAHHALLIHISTDYVFDGNSCLPYRETDRP
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCCC
APLGIYGKSKRAAEELIFEIAPSFLILRTSWLYSLYGHNFLKTMLRLGAEKDVIKVIFDQ
CCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEECC
TGTPTYAGDLARAIMTILGKVRLDEQYGALYHYSNEGVCSWYDFAVAIMALKKLPCKVLP
CCCCCHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEE
IETTEYPLPSPRPFFSVLNKSAIKRDWGLVIPYWRDSLESVLLAEEDAGL
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]