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The map label for this gene is cqsA [H]

Identifier: 119357750

GI number: 119357750

Start: 2268058

End: 2269293

Strand: Reverse

Name: cqsA [H]

Synonym: Cpha266_1956

Alternate gene names: 119357750

Gene position: 2269293-2268058 (Counterclockwise)

Preceding gene: 119357752

Following gene: 119357749

Centisome position: 72.41

GC content: 45.79

Gene sequence:

>1236_bases
ATGAGCTGTTCTTTAACAAGTGCCGGAGTCGATTATGGACAGAGACGCTCTTTATTGCATGTTCCCGATTTTCTTGAAAA
GCGTCTTCATGCTTTTGACGAAGCTGTAAAGTTCAGCAGAACAGGAAAACCTGTCGTAGTAGGCAATAAACCTGGCCAAG
GATCAATTATTCTTCAAAGCAATGACTATCTGAATGTTTCCATGCATCCTGATATTTCGCTTGCCCAGATTGCCAGGCTT
CAAAATACTGAGAAGGAACTGGTTATGTCGGCGGTGTTTCTTCATGAAGGGAGCGACAAGGAGTTATTTGAAAACTCCAT
GGCTGATTTTACGGGTTTTGAAAGTTCCATATTATGCCAGTCCGGATGGTCTGCAAATATCGGTCTCATGCAGGCAATTG
CCGATCAGAATACGCCGGTTTATATCGATTTTTTCACACATATGTCTCTCTGGGAGGGGATTAAATCTTCCGGAGCACCT
TTTTATTCTTTTATGCACAACGATGTCGAACATCTTGAACGGCTTGTGAAACAGCATGGTAAAGGTATTATTCTTGTGGA
CTCTCTCTATAGTACAACAGGAGACTTCGCTCCTCTCGTGGATATTATAGATATTGCGACCCGTTACGATTGCGTTTCAG
TGGTTGATGAATCTCATTCGCTTGGTACTCATGGCGATCATGGCTCAGGTCTTGTTGCTTCGCTCGGGCTTACCGGGAGA
GTTCATTTCATCACGGCAAGTCTTGCAAAAGCATTTGCCGGTCGGGCCGGTATTATTTTTTGTTCGAAGCGATTTTCTCA
GTATTTCCCCTTTATCGCATATCCGGCCATTTTCAGCTCGACGTTACTTCCGCATGAAATCGCCGGATTGGCAGCCACAC
TGAAAGTCATTATCGATGGCGATGAGCGCCGTAAACAATTGCATGCGCATGCGGAATATCTTCGTGAAGGTCTACGCGGT
CTCGGTTATTCGATTGCCAGCGAGTCGCAGATAATCGGGTTAGAGCCGGGAGAAGAGTCCAATATGGAGATTCTTCGCGA
TGCCCTTGAAGATAGGAATGTGTATGGATCTGTCTTTTGTGCGCCGGCAACTCCCAAAAACCGTTCCCTCATGCGCTTTT
CCCTGCACAGCAGTCTTGAAAAGGATGATTTAGAGCGTGTTCTCGCTGTTTGCGAATCAATACGTGACGATGTGGGAATG
TGGAACTGGAAGTCGACGAGGCGGAAAAAAGGATAG

Upstream 100 bases:

>100_bases
CATGTTTCGAATATATTTAAAATAACCTCAATGCAATCGATCGTTTTTTTATCAACAACCTGAAACAATACAGGGTTTAG
AAACCCATAGGAGATGCGTG

Downstream 100 bases:

>100_bases
GCGCGGGATTGTTGTTATGTTCAATAGCATGTAAGTTTTGATACAATTTCAGTTGCAACCTGAATTCCTGGAGAATTTTA
ACGATGCCGGGTCAAGGACG

Product: hypothetical protein

Products: Acetyl-CoA; Glycine [C]

Alternate protein names: Cholerae quorum-sensing autoinducer [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MSCSLTSAGVDYGQRRSLLHVPDFLEKRLHAFDEAVKFSRTGKPVVVGNKPGQGSIILQSNDYLNVSMHPDISLAQIARL
QNTEKELVMSAVFLHEGSDKELFENSMADFTGFESSILCQSGWSANIGLMQAIADQNTPVYIDFFTHMSLWEGIKSSGAP
FYSFMHNDVEHLERLVKQHGKGIILVDSLYSTTGDFAPLVDIIDIATRYDCVSVVDESHSLGTHGDHGSGLVASLGLTGR
VHFITASLAKAFAGRAGIIFCSKRFSQYFPFIAYPAIFSSTLLPHEIAGLAATLKVIIDGDERRKQLHAHAEYLREGLRG
LGYSIASESQIIGLEPGEESNMEILRDALEDRNVYGSVFCAPATPKNRSLMRFSLHSSLEKDDLERVLAVCESIRDDVGM
WNWKSTRRKKG

Sequences:

>Translated_411_residues
MSCSLTSAGVDYGQRRSLLHVPDFLEKRLHAFDEAVKFSRTGKPVVVGNKPGQGSIILQSNDYLNVSMHPDISLAQIARL
QNTEKELVMSAVFLHEGSDKELFENSMADFTGFESSILCQSGWSANIGLMQAIADQNTPVYIDFFTHMSLWEGIKSSGAP
FYSFMHNDVEHLERLVKQHGKGIILVDSLYSTTGDFAPLVDIIDIATRYDCVSVVDESHSLGTHGDHGSGLVASLGLTGR
VHFITASLAKAFAGRAGIIFCSKRFSQYFPFIAYPAIFSSTLLPHEIAGLAATLKVIIDGDERRKQLHAHAEYLREGLRG
LGYSIASESQIIGLEPGEESNMEILRDALEDRNVYGSVFCAPATPKNRSLMRFSLHSSLEKDDLERVLAVCESIRDDVGM
WNWKSTRRKKG
>Mature_410_residues
SCSLTSAGVDYGQRRSLLHVPDFLEKRLHAFDEAVKFSRTGKPVVVGNKPGQGSIILQSNDYLNVSMHPDISLAQIARLQ
NTEKELVMSAVFLHEGSDKELFENSMADFTGFESSILCQSGWSANIGLMQAIADQNTPVYIDFFTHMSLWEGIKSSGAPF
YSFMHNDVEHLERLVKQHGKGIILVDSLYSTTGDFAPLVDIIDIATRYDCVSVVDESHSLGTHGDHGSGLVASLGLTGRV
HFITASLAKAFAGRAGIIFCSKRFSQYFPFIAYPAIFSSTLLPHEIAGLAATLKVIIDGDERRKQLHAHAEYLREGLRGL
GYSIASESQIIGLEPGEESNMEILRDALEDRNVYGSVFCAPATPKNRSLMRFSLHSSLEKDDLERVLAVCESIRDDVGMW
NWKSTRRKKG

Specific function: Required for the synthesis of the quorum-sensing autoinducer CAI-1 ((S)-3-hydroxytridecan-4-one) which probably functions as an intragenus signal [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=360, Percent_Identity=25.8333333333333, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI284448556, Length=339, Percent_Identity=25.3687315634218, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI83977440, Length=349, Percent_Identity=26.0744985673352, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI83977442, Length=349, Percent_Identity=26.0744985673352, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI83977444, Length=349, Percent_Identity=26.0744985673352, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI4502025, Length=328, Percent_Identity=26.5243902439024, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI40316939, Length=328, Percent_Identity=26.5243902439024, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI4758668, Length=407, Percent_Identity=25.3071253071253, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI119220554, Length=335, Percent_Identity=26.865671641791, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI5454084, Length=176, Percent_Identity=26.1363636363636, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790046, Length=350, Percent_Identity=27.4285714285714, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1786993, Length=337, Percent_Identity=27.5964391691395, Blast_Score=131, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI71994529, Length=362, Percent_Identity=25.414364640884, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17560914, Length=307, Percent_Identity=25.4071661237785, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560912, Length=307, Percent_Identity=25.4071661237785, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI32566772, Length=319, Percent_Identity=23.1974921630094, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71982625, Length=363, Percent_Identity=24.2424242424242, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71982617, Length=363, Percent_Identity=23.6914600550964, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320267, Length=318, Percent_Identity=25.4716981132075, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320438, Length=348, Percent_Identity=23.2758620689655, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137420, Length=317, Percent_Identity=28.391167192429, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24662918, Length=337, Percent_Identity=25.5192878338279, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI17136286, Length=335, Percent_Identity=24.7761194029851, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24653280, Length=324, Percent_Identity=23.7654320987654, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24653276, Length=324, Percent_Identity=23.7654320987654, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24653278, Length=324, Percent_Identity=23.7654320987654, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.3.1.29 [C]

Molecular weight: Translated: 45413; Mature: 45282

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCSLTSAGVDYGQRRSLLHVPDFLEKRLHAFDEAVKFSRTGKPVVVGNKPGQGSIILQS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEC
NDYLNVSMHPDISLAQIARLQNTEKELVMSAVFLHEGSDKELFENSMADFTGFESSILCQ
CCEEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEC
SGWSANIGLMQAIADQNTPVYIDFFTHMSLWEGIKSSGAPFYSFMHNDVEHLERLVKQHG
CCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
KGIILVDSLYSTTGDFAPLVDIIDIATRYDCVSVVDESHSLGTHGDHGSGLVASLGLTGR
CCEEEEECHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCC
VHFITASLAKAFAGRAGIIFCSKRFSQYFPFIAYPAIFSSTLLPHEIAGLAATLKVIIDG
HHHHHHHHHHHHCCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHEEEEEECC
DERRKQLHAHAEYLREGLRGLGYSIASESQIIGLEPGEESNMEILRDALEDRNVYGSVFC
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEE
APATPKNRSLMRFSLHSSLEKDDLERVLAVCESIRDDVGMWNWKSTRRKKG
CCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCC
>Mature Secondary Structure 
SCSLTSAGVDYGQRRSLLHVPDFLEKRLHAFDEAVKFSRTGKPVVVGNKPGQGSIILQS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEC
NDYLNVSMHPDISLAQIARLQNTEKELVMSAVFLHEGSDKELFENSMADFTGFESSILCQ
CCEEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEC
SGWSANIGLMQAIADQNTPVYIDFFTHMSLWEGIKSSGAPFYSFMHNDVEHLERLVKQHG
CCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
KGIILVDSLYSTTGDFAPLVDIIDIATRYDCVSVVDESHSLGTHGDHGSGLVASLGLTGR
CCEEEEECHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCC
VHFITASLAKAFAGRAGIIFCSKRFSQYFPFIAYPAIFSSTLLPHEIAGLAATLKVIIDG
HHHHHHHHHHHHCCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHEEEEEECC
DERRKQLHAHAEYLREGLRGLGYSIASESQIIGLEPGEESNMEILRDALEDRNVYGSVFC
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEE
APATPKNRSLMRFSLHSSLEKDDLERVLAVCESIRDDVGMWNWKSTRRKKG
CCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5-phosphate mol per mol subunit, enzyme, [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 12 {butyryl-CoA}} 0.08 {propionyl-CoA}} 0.059 {acetyl-CoA}} [C]

Substrates: L-2-Amino-3-oxobutanoate; Coenzyme A [C]

Specific reaction: L-2-Amino-3-oxobutanoate + Coenzyme A --> Acetyl-CoA + Glycine [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 2-Cyclohexanedione; 2, 3-Butanedione; 4-(Oxoacetyl) phenoxyacetic acid; Aminomalonic acid; Aminomethyl phosphonic acid; Aminooxyacetate; Ba2+; Ca2+; Cd2+; CoA; Cu2+; DTT; Glutathione; Hg2+; Hydroxy l amine; IAA; L-Cysteine; Mn2+; PCMB, NEM, glycinemeth

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301; 12176318 [H]