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The map label for this gene is mdh

Identifier: 119357696

GI number: 119357696

Start: 2206741

End: 2207673

Strand: Direct

Name: mdh

Synonym: Cpha266_1900

Alternate gene names: 119357696

Gene position: 2206741-2207673 (Clockwise)

Preceding gene: 119357695

Following gene: 119357703

Centisome position: 70.42

GC content: 49.84

Gene sequence:

>933_bases
ATGAAAATATCCGTTATTGGAGCTGGTAATGTCGGGGCTACTGCTGCGCATCGCCTTGCAGAAAAACAGCTCGCACATGA
AGTTGTCCTGATTGATATTGTAGAGGGCATTCCGCAGGGAAAAGCGCTTGACATGTACGAATCCGGTCCGGTAGGACTTT
TTGATACCGCTATCCATGGATCAAACGATTATATGGCATCCGCTGATTCGGATATCGTCCTGATTACCGCCGGGCTTGCA
AGAAAACCGGGCATGACCAGAGAAGATCTGCTCATGAAAAATGCGGGAATCGTCAAAGAGGTTACCGACCAGGTAATGAA
ACACTCATCGAATCCGATTCTTGTGATGGTATCCAACCCACTCGATGTCATGACCTTTGTTGCTCATGCGTCAAGCGGTC
TTGGTAAAGAACGGGTGATCGGCATGGCTGGCGTTCTCGATGCTGCAAGGTTCAGAAGCTTCATTGCAGAAGAACTGAAC
GTTTCGATGCAGGACGTCAATGCCTTTGTCCTGGGGGGACATGGTGACTCCATGGTACCTGTGGTTAAATATACATCGGT
TGCAGGCATACCGATCACCGAGCTGCTCTCGCAGGAAAAAATTGACGCGCTTGTCGAACGTACTCGCAACGGCGGTGTTG
AAATCGTCAACTATCTCAAAAACGGTTCGGCATTCTATGCCCCCGCAGCTTCCGCTGTGGAGATGATAGAGGCTATCGTC
AAAGACCGCAAACGGATACTTGCCTGTACAACCCTGCTTGAAGGAGAGTACGGCATCAATAACGTTTTTTGCGGCGTTCC
GGTAAAGATAGGCAAAAACGGTGTTGAAGAGATTCTCGAAATCAATCTTGCGCCGGCTGAACTCGACGCACTGAAGCATT
CCGCCAGCCTCGTGCAGGAAAACTGCAAAAGCCTTGAAGCGCTTCTCGCCTGA

Upstream 100 bases:

>100_bases
CCCTGAGCCGTCTCTTTACAAGGCGGCTTTTATTTTGTTTGACAAAAAGCTCAAACCGAAACTTCATCACCGATTACTCC
AAACAAAAATCCTCAGTGCT

Downstream 100 bases:

>100_bases
AGGCAATAACAAGGTATTCAAAAAAAAAAGCGGCTCTCAAACGGCCGCTTTTTTTATTGCTCTCTCACCTCTCCCGTATC
GGCAGATCCGGCTCTTCGTG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKISVIGAGNVGATAAHRLAEKQLAHEVVLIDIVEGIPQGKALDMYESGPVGLFDTAIHGSNDYMASADSDIVLITAGLA
RKPGMTREDLLMKNAGIVKEVTDQVMKHSSNPILVMVSNPLDVMTFVAHASSGLGKERVIGMAGVLDAARFRSFIAEELN
VSMQDVNAFVLGGHGDSMVPVVKYTSVAGIPITELLSQEKIDALVERTRNGGVEIVNYLKNGSAFYAPAASAVEMIEAIV
KDRKRILACTTLLEGEYGINNVFCGVPVKIGKNGVEEILEINLAPAELDALKHSASLVQENCKSLEALLA

Sequences:

>Translated_310_residues
MKISVIGAGNVGATAAHRLAEKQLAHEVVLIDIVEGIPQGKALDMYESGPVGLFDTAIHGSNDYMASADSDIVLITAGLA
RKPGMTREDLLMKNAGIVKEVTDQVMKHSSNPILVMVSNPLDVMTFVAHASSGLGKERVIGMAGVLDAARFRSFIAEELN
VSMQDVNAFVLGGHGDSMVPVVKYTSVAGIPITELLSQEKIDALVERTRNGGVEIVNYLKNGSAFYAPAASAVEMIEAIV
KDRKRILACTTLLEGEYGINNVFCGVPVKIGKNGVEEILEINLAPAELDALKHSASLVQENCKSLEALLA
>Mature_310_residues
MKISVIGAGNVGATAAHRLAEKQLAHEVVLIDIVEGIPQGKALDMYESGPVGLFDTAIHGSNDYMASADSDIVLITAGLA
RKPGMTREDLLMKNAGIVKEVTDQVMKHSSNPILVMVSNPLDVMTFVAHASSGLGKERVIGMAGVLDAARFRSFIAEELN
VSMQDVNAFVLGGHGDSMVPVVKYTSVAGIPITELLSQEKIDALVERTRNGGVEIVNYLKNGSAFYAPAASAVEMIEAIV
KDRKRILACTTLLEGEYGINNVFCGVPVKIGKNGVEEILEINLAPAELDALKHSASLVQENCKSLEALLA

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=313, Percent_Identity=34.8242811501597, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI221136809, Length=313, Percent_Identity=34.8242811501597, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI15082234, Length=307, Percent_Identity=33.8762214983713, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI260099723, Length=312, Percent_Identity=35.2564102564103, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI5031857, Length=312, Percent_Identity=35.2564102564103, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI291575128, Length=315, Percent_Identity=33.3333333333333, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI4557032, Length=315, Percent_Identity=33.3333333333333, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI9257228, Length=312, Percent_Identity=32.0512820512821, Blast_Score=164, Evalue=8e-41,
Organism=Homo sapiens, GI4504973, Length=312, Percent_Identity=32.0512820512821, Blast_Score=164, Evalue=8e-41,
Organism=Homo sapiens, GI260099725, Length=194, Percent_Identity=40.2061855670103, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI260099727, Length=194, Percent_Identity=40.2061855670103, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI207028494, Length=313, Percent_Identity=27.7955271565495, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI103472011, Length=300, Percent_Identity=25.3333333333333, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI21735621, Length=298, Percent_Identity=27.1812080536913, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI103472015, Length=179, Percent_Identity=26.2569832402235, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789632, Length=320, Percent_Identity=30.9375, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17535107, Length=295, Percent_Identity=30.1694915254237, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17554310, Length=298, Percent_Identity=29.8657718120805, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322765, Length=226, Percent_Identity=32.3008849557522, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320125, Length=260, Percent_Identity=28.4615384615385, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324446, Length=174, Percent_Identity=32.183908045977, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17136226, Length=307, Percent_Identity=32.5732899022801, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI45550422, Length=309, Percent_Identity=30.0970873786408, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24647881, Length=298, Percent_Identity=30.5369127516779, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24663599, Length=231, Percent_Identity=31.6017316017316, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24663595, Length=249, Percent_Identity=25.7028112449799, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_CHLPD (A1BHN9)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_912340.1
- ProteinModelPortal:   A1BHN9
- SMR:   A1BHN9
- STRING:   A1BHN9
- GeneID:   4570859
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1900
- NMPDR:   fig|290317.7.peg.2005
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   YAVYKES
- ProtClustDB:   PRK06223
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 32911; Mature: 32911

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: ACT_SITE 174-174 BINDING 32-32 BINDING 81-81 BINDING 87-87 BINDING 94-94 BINDING 119-119 BINDING 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISVIGAGNVGATAAHRLAEKQLAHEVVLIDIVEGIPQGKALDMYESGPVGLFDTAIHG
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCEEEHEEEC
SNDYMASADSDIVLITAGLARKPGMTREDLLMKNAGIVKEVTDQVMKHSSNPILVMVSNP
CCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCC
LDVMTFVAHASSGLGKERVIGMAGVLDAARFRSFIAEELNVSMQDVNAFVLGGHGDSMVP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCCCE
VVKYTSVAGIPITELLSQEKIDALVERTRNGGVEIVNYLKNGSAFYAPAASAVEMIEAIV
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCHHHHHHHHHHHH
KDRKRILACTTLLEGEYGINNVFCGVPVKIGKNGVEEILEINLAPAELDALKHSASLVQE
HHHHHHHHHHHHHCCCCCCCCEEECCCHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NCKSLEALLA
HHHHHHHHHC
>Mature Secondary Structure
MKISVIGAGNVGATAAHRLAEKQLAHEVVLIDIVEGIPQGKALDMYESGPVGLFDTAIHG
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCEEEHEEEC
SNDYMASADSDIVLITAGLARKPGMTREDLLMKNAGIVKEVTDQVMKHSSNPILVMVSNP
CCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCC
LDVMTFVAHASSGLGKERVIGMAGVLDAARFRSFIAEELNVSMQDVNAFVLGGHGDSMVP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCCCE
VVKYTSVAGIPITELLSQEKIDALVERTRNGGVEIVNYLKNGSAFYAPAASAVEMIEAIV
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCHHHHHHHHHHHH
KDRKRILACTTLLEGEYGINNVFCGVPVKIGKNGVEEILEINLAPAELDALKHSASLVQE
HHHHHHHHHHHHHCCCCCCCCEEECCCHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NCKSLEALLA
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA