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The map label for this gene is hisF

Identifier: 119357703

GI number: 119357703

Start: 2214683

End: 2215438

Strand: Direct

Name: hisF

Synonym: Cpha266_1907

Alternate gene names: 119357703

Gene position: 2214683-2215438 (Clockwise)

Preceding gene: 119357696

Following gene: 119357707

Centisome position: 70.67

GC content: 51.85

Gene sequence:

>756_bases
ATGTTAGCAAAAAGAATCATTCCCTGTCTCGACGTTACGGGCGGCAGGGTCGTCAAGGGCATCAACTTCGAGGGTTTACG
AGATGCCGGTTCAATTCTTGAACAGGCCCGCTTTTACAACAACGAACTTGCCGACGAACTGGTTTTTCTCGACATTTCAG
CATCGCTTGAGTCACGAAAAACAACCCTCGAAGAGGTCATGAAAGTTTCAGGGGAAGTTTTTATTCCGCTTACCGTAGGA
GGCGGCATCAATTCCGTTGAACGAGCAAAGGATGTTTTTCTGCATGGAGCTGACAAGGTTTCGGTCAACACGGCAGCAGT
CAACCATCCTGAACTGATCTCCCGCATCGCTGAAAAGTATGGATCCCAGGCTGTGGTAGTGGCTATCGATATCAAGAAAA
TCGGCAGCGATCACATGGTGCACACCCATTCAGGAAAAAATCCAACGGCATATGAAGCCCTTGAATGGGCACACAAGGTA
CAGGAACTCGGGGCCGGGGAGATTCTGCTCACAAGCATGGATCGCGACGGAACCCGGGAGGGGTACGACAACGATATTCT
CGCTCGCGTTTCAACCTCGGTTCACATTCCCGTCATCGCTTCGGGAGGTGCCGGAAACCTCGAACACCTCTATGAGGGTT
TCACGATAGGCCATGCCGATGCCGCCCTTGCAGCCTCAATCTTTCATTTCAGACAGTACTCAATCCGGCAGGCCAAGCAG
TACCTCCGCGACAGGGGCATAGAGGTGAGGCTGTAA

Upstream 100 bases:

>100_bases
AATATAATACCTTTCAAATATTGTTGAAGAGTTTAACTTTATTGATTTCTTTCGAGTCGTTACCATTCTACAGAGCAATT
TACTTTCAGCAGAAAAAACC

Downstream 100 bases:

>100_bases
AGAGAACCGGACAAGAGATGGTGGTTCCCGGGCAATCCCGAATTACCTGCATGGAGATCACTGCTTTTATGAAACAGAAT
GGAGCGGTTGAGATCAGCGT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLAKRIIPCLDVTGGRVVKGINFEGLRDAGSILEQARFYNNELADELVFLDISASLESRKTTLEEVMKVSGEVFIPLTVG
GGINSVERAKDVFLHGADKVSVNTAAVNHPELISRIAEKYGSQAVVVAIDIKKIGSDHMVHTHSGKNPTAYEALEWAHKV
QELGAGEILLTSMDRDGTREGYDNDILARVSTSVHIPVIASGGAGNLEHLYEGFTIGHADAALAASIFHFRQYSIRQAKQ
YLRDRGIEVRL

Sequences:

>Translated_251_residues
MLAKRIIPCLDVTGGRVVKGINFEGLRDAGSILEQARFYNNELADELVFLDISASLESRKTTLEEVMKVSGEVFIPLTVG
GGINSVERAKDVFLHGADKVSVNTAAVNHPELISRIAEKYGSQAVVVAIDIKKIGSDHMVHTHSGKNPTAYEALEWAHKV
QELGAGEILLTSMDRDGTREGYDNDILARVSTSVHIPVIASGGAGNLEHLYEGFTIGHADAALAASIFHFRQYSIRQAKQ
YLRDRGIEVRL
>Mature_251_residues
MLAKRIIPCLDVTGGRVVKGINFEGLRDAGSILEQARFYNNELADELVFLDISASLESRKTTLEEVMKVSGEVFIPLTVG
GGINSVERAKDVFLHGADKVSVNTAAVNHPELISRIAEKYGSQAVVVAIDIKKIGSDHMVHTHSGKNPTAYEALEWAHKV
QELGAGEILLTSMDRDGTREGYDNDILARVSTSVHIPVIASGGAGNLEHLYEGFTIGHADAALAASIFHFRQYSIRQAKQ
YLRDRGIEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=44.9612403100775, Blast_Score=216, Evalue=9e-58,
Organism=Escherichia coli, GI87082028, Length=210, Percent_Identity=25.2380952380952, Blast_Score=61, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=33.7579617834395, Blast_Score=149, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_CHLPD (A1BHP6)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_912347.1
- ProteinModelPortal:   A1BHP6
- SMR:   A1BHP6
- STRING:   A1BHP6
- GeneID:   4570866
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1907
- NMPDR:   fig|290317.7.peg.2013
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27365; Mature: 27365

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVTGGRVVKGINFEGLRDAGSILEQARFYNNELADELVFLDISASLESRK
CCHHHCCCHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHH
TTLEEVMKVSGEVFIPLTVGGGINSVERAKDVFLHGADKVSVNTAAVNHPELISRIAEKY
HHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
GSQAVVVAIDIKKIGSDHMVHTHSGKNPTAYEALEWAHKVQELGAGEILLTSMDRDGTRE
CCCEEEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDNDILARVSTSVHIPVIASGGAGNLEHLYEGFTIGHADAALAASIFHFRQYSIRQAKQ
CCCCCHHEEECCEEEEEEEECCCCCCHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHH
YLRDRGIEVRL
HHHHCCCEECC
>Mature Secondary Structure
MLAKRIIPCLDVTGGRVVKGINFEGLRDAGSILEQARFYNNELADELVFLDISASLESRK
CCHHHCCCHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHH
TTLEEVMKVSGEVFIPLTVGGGINSVERAKDVFLHGADKVSVNTAAVNHPELISRIAEKY
HHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
GSQAVVVAIDIKKIGSDHMVHTHSGKNPTAYEALEWAHKVQELGAGEILLTSMDRDGTRE
CCCEEEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDNDILARVSTSVHIPVIASGGAGNLEHLYEGFTIGHADAALAASIFHFRQYSIRQAKQ
CCCCCHHEEECCEEEEEEEECCCCCCHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHH
YLRDRGIEVRL
HHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA