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The map label for this gene is rimO

Identifier: 119357557

GI number: 119357557

Start: 2002056

End: 2003354

Strand: Direct

Name: rimO

Synonym: Cpha266_1760

Alternate gene names: 119357557

Gene position: 2002056-2003354 (Clockwise)

Preceding gene: 119357556

Following gene: 119357558

Centisome position: 63.88

GC content: 51.19

Gene sequence:

>1299_bases
ATGAAAAAACATAACGTCTTTCTGCTAAGCCTCGGATGCTCAAAAAACACTGTAGACTCCGAACGCCTCATGGCACAGGC
GGAGGCATCCGGCATAACGTTTACCGAAGAGGCCGACCTTGCCGATACCATCCTTATTAACACCTGCGGGTTCATCGAAG
ATGCCAAGGAAGAGTCGATTGCTGAAATACTTGCAGCCGTTGAAAAAAAAACTCAAGGTATTGTTTCAGGCGTTTACGTT
ATGGGGTGCCTCAGCGAACTCTACCGCACGGAAATGAGAGAAGAGCTTCCGGAGATTGACGGATTTTTCGGCACCCGTGA
ACTGCCTGCCCTGCTTCAGGCAATCGGAGCTCAATACCGTGACGAGCTGTATGACCATCGATCGCTCCTTACACCACCGC
ATATTTCCTATCTTAAAATTGCCGAAGGTTGCAATCGCTCATGTTCCTTCTGCTCGATCCCGAAAATCAGGGGACGGTAC
AGGAGCCAGCCAATGGAGCAACTGCTTCGGGAAGCCGCCCTTTTACAGAAAAAAGGGGTTCGGGAGCTCAATCTCATCGC
ACAGGACACCAGCATCTACGGAAGAGATCTCTACGGCACACCCATGCTCCGGGAGCTGCTTGTTCGATTATCCGACATGG
AGTTCCGCTGGATTCGTCTGCTCTATGCCTATCCTCTCGACTTTCCGCTCGAAGTGATTACCGCCATGAGCGAGCGAAAA
AATATCTGCAACTACCTCGATCTTCCCTTGCAGCACTGCAATGACCGAATTCTTCGATCAATGAACCGGGGAATCACAAA
AACGGAAACCGTAAGGCTTCTTGACACGATCAGAGCCGCAAACCCGGACATCAGGCTTCGCACCACCATGCTTGTCGGAT
TTCCGGGAGAAACCAGAGCGGAGTTCGATGAACTCATGCAGTTTATCGAAACGATGCGCTTCGACAGACTCGGCTGCTTC
CCCTACTGCCATGAAGAACACGCCCCATCCTATGCGCTTGAAGATACGGTCAAAGCGGAAGAGAAGGAGGAACGCAGAGC
TGAGTTGATGGAACTTCAGGAGACCATTGCCAAAGAGAACAATCAGCTATTCGAAGGAAAGGAACTTACCGTGCTCATCG
ACCAGATTGAGGGCGATATCGCTATAGCGCGAACGGAATACGACGCTCCAGAAGTTGACAACGAATGTTATCTGACCACC
GGCTCACTCAGGGTCGGAACAGGAGAGTTCTGCACCGCACATATTTCAGAGAGCTCCGCCTACGAACTGCACGGCACGAT
CACCGCCGTTAATGGATAA

Upstream 100 bases:

>100_bases
ATCTCCTTCTACCTCTGGCTCACGACGACTGCACGCGAACAGATTCAGAACGGCACCTTCACCGAATGGAAAAACGACTT
TCTCAGCAGATTCAACAGCA

Downstream 100 bases:

>100_bases
AGAGCATAAGCTCCGGACCTCGAAGAAAGATCCCTCTGAGTTTACAGGTTTTTCGAATCCTTATGGATCGGTAAAACTCA
CTGGAGACCATCGCCACTTC

Product: MiaB-like tRNA modifying enzyme YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKKHNVFLLSLGCSKNTVDSERLMAQAEASGITFTEEADLADTILINTCGFIEDAKEESIAEILAAVEKKTQGIVSGVYV
MGCLSELYRTEMREELPEIDGFFGTRELPALLQAIGAQYRDELYDHRSLLTPPHISYLKIAEGCNRSCSFCSIPKIRGRY
RSQPMEQLLREAALLQKKGVRELNLIAQDTSIYGRDLYGTPMLRELLVRLSDMEFRWIRLLYAYPLDFPLEVITAMSERK
NICNYLDLPLQHCNDRILRSMNRGITKTETVRLLDTIRAANPDIRLRTTMLVGFPGETRAEFDELMQFIETMRFDRLGCF
PYCHEEHAPSYALEDTVKAEEKEERRAELMELQETIAKENNQLFEGKELTVLIDQIEGDIAIARTEYDAPEVDNECYLTT
GSLRVGTGEFCTAHISESSAYELHGTITAVNG

Sequences:

>Translated_432_residues
MKKHNVFLLSLGCSKNTVDSERLMAQAEASGITFTEEADLADTILINTCGFIEDAKEESIAEILAAVEKKTQGIVSGVYV
MGCLSELYRTEMREELPEIDGFFGTRELPALLQAIGAQYRDELYDHRSLLTPPHISYLKIAEGCNRSCSFCSIPKIRGRY
RSQPMEQLLREAALLQKKGVRELNLIAQDTSIYGRDLYGTPMLRELLVRLSDMEFRWIRLLYAYPLDFPLEVITAMSERK
NICNYLDLPLQHCNDRILRSMNRGITKTETVRLLDTIRAANPDIRLRTTMLVGFPGETRAEFDELMQFIETMRFDRLGCF
PYCHEEHAPSYALEDTVKAEEKEERRAELMELQETIAKENNQLFEGKELTVLIDQIEGDIAIARTEYDAPEVDNECYLTT
GSLRVGTGEFCTAHISESSAYELHGTITAVNG
>Mature_432_residues
MKKHNVFLLSLGCSKNTVDSERLMAQAEASGITFTEEADLADTILINTCGFIEDAKEESIAEILAAVEKKTQGIVSGVYV
MGCLSELYRTEMREELPEIDGFFGTRELPALLQAIGAQYRDELYDHRSLLTPPHISYLKIAEGCNRSCSFCSIPKIRGRY
RSQPMEQLLREAALLQKKGVRELNLIAQDTSIYGRDLYGTPMLRELLVRLSDMEFRWIRLLYAYPLDFPLEVITAMSERK
NICNYLDLPLQHCNDRILRSMNRGITKTETVRLLDTIRAANPDIRLRTTMLVGFPGETRAEFDELMQFIETMRFDRLGCF
PYCHEEHAPSYALEDTVKAEEKEERRAELMELQETIAKENNQLFEGKELTVLIDQIEGDIAIARTEYDAPEVDNECYLTT
GSLRVGTGEFCTAHISESSAYELHGTITAVNG

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=483, Percent_Identity=26.7080745341615, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI28872782, Length=483, Percent_Identity=26.7080745341615, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI93277076, Length=403, Percent_Identity=25.8064516129032, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1787057, Length=440, Percent_Identity=36.5909090909091, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI1786882, Length=372, Percent_Identity=30.1075268817204, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17553146, Length=415, Percent_Identity=26.0240963855422, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71996771, Length=224, Percent_Identity=30.3571428571429, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356207, Length=472, Percent_Identity=25.2118644067797, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922432, Length=401, Percent_Identity=23.4413965087282, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_CHLPD (A1BHA0)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_912201.1
- ProteinModelPortal:   A1BHA0
- SMR:   A1BHA0
- STRING:   A1BHA0
- GeneID:   4570104
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1760
- NMPDR:   fig|290317.7.peg.1842
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK637740
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49093; Mature: 49093

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHNVFLLSLGCSKNTVDSERLMAQAEASGITFTEEADLADTILINTCGFIEDAKEESI
CCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHCCCHHHHHHHHH
AEILAAVEKKTQGIVSGVYVMGCLSELYRTEMREELPEIDGFFGTRELPALLQAIGAQYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
DELYDHRSLLTPPHISYLKIAEGCNRSCSFCSIPKIRGRYRSQPMEQLLREAALLQKKGV
HHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCC
RELNLIAQDTSIYGRDLYGTPMLRELLVRLSDMEFRWIRLLYAYPLDFPLEVITAMSERK
HHHHHEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
NICNYLDLPLQHCNDRILRSMNRGITKTETVRLLDTIRAANPDIRLRTTMLVGFPGETRA
HHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHH
EFDELMQFIETMRFDRLGCFPYCHEEHAPSYALEDTVKAEEKEERRAELMELQETIAKEN
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NQLFEGKELTVLIDQIEGDIAIARTEYDAPEVDNECYLTTGSLRVGTGEFCTAHISESSA
CCEECCCCEEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCEEECCCCCEEEECCCCCC
YELHGTITAVNG
EEEEEEEEEECC
>Mature Secondary Structure
MKKHNVFLLSLGCSKNTVDSERLMAQAEASGITFTEEADLADTILINTCGFIEDAKEESI
CCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHCCCHHHHHHHHH
AEILAAVEKKTQGIVSGVYVMGCLSELYRTEMREELPEIDGFFGTRELPALLQAIGAQYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
DELYDHRSLLTPPHISYLKIAEGCNRSCSFCSIPKIRGRYRSQPMEQLLREAALLQKKGV
HHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCC
RELNLIAQDTSIYGRDLYGTPMLRELLVRLSDMEFRWIRLLYAYPLDFPLEVITAMSERK
HHHHHEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
NICNYLDLPLQHCNDRILRSMNRGITKTETVRLLDTIRAANPDIRLRTTMLVGFPGETRA
HHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHH
EFDELMQFIETMRFDRLGCFPYCHEEHAPSYALEDTVKAEEKEERRAELMELQETIAKEN
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NQLFEGKELTVLIDQIEGDIAIARTEYDAPEVDNECYLTTGSLRVGTGEFCTAHISESSA
CCEECCCCEEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCEEECCCCCEEEECCCCCC
YELHGTITAVNG
EEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA