Click here to switch to the map view.

The map label for this gene is tgt [H]

Identifier: 119357556

GI number: 119357556

Start: 2000936

End: 2002069

Strand: Direct

Name: tgt [H]

Synonym: Cpha266_1759

Alternate gene names: 119357556

Gene position: 2000936-2002069 (Clockwise)

Preceding gene: 119357555

Following gene: 119357557

Centisome position: 63.85

GC content: 52.12

Gene sequence:

>1134_bases
ATGAATTTTACTCTTACTGCGACTGATACGAAAAGCTCGGCAAGATGCGGCGTGCTTGAAACCTCTCACGGAACCATCCC
GACCCCGATTTTCATGCCGGTTGGTACCCGCGCAAGCGTTAAATCGCTTGAACCGCATGAGCTTACTGAACTGGGGGTAA
GCATCATCCTTGCCAACACCTACCATCTCTATCTCAGACCAGGAAATGATATTCTCCGAAAAGCCGGCGGCGTGCACCGG
TTCATGAACTGGAACGGCCCGCTCCTCACCGACAGCGGCGGGTACCAGGTTTACTCACTCTCTGATCTTCGAAAAATCAC
CGAAGAAGGTGTGACCTTCCGCTCACACCTCGATGGATCAAAACAGCAGTTTACTCCTGAAAACGTAATTGATACCGAGC
GCATTATCGGCAGCGATATCATAATGCCGCTTGACGAGTGCCCACCGTCAACCGCTGATCGGGAATATGTGCAGACCTCA
GGAGAACTTACTATCCGCTGGGCAGAACGGGCAAAAAAAAGGTTTGTCTCCACCTCCCCGCTCTACGGGCACAGCCAGTT
CCTTTTCGGCATTACCCAGGGCGGCATTCATGCCGACCTTCGAACAATCTCAACCAAAGCTCTGGTGGATCTTGATTTCG
ACGGCTACGCCATTGGCGGCATGGCCGTAGGCGAACCTGCCGAAGCGATGTACAGGGTGCTCGAGCTTTCAGATATGCTG
CTTCCCGCACGCAAACCCCGCTATCTCATGGGTGTGGGCACACCGGAAAACATTCTGAACGCTATCGAGCGAGGCGTCGA
CATGTTCGACTGCGTCATTCCGACTCGCGAAGGACGAAACGGCAGAGTCTATACCCGCAACGGAACAATGAACCTGCGGG
CAGCGAAATATGCCGCAGATTTCCGTCCTCTGGATGAAGGATTTGATAATGAGGTGTGCAGGAATTTCTCCCGAGCCTAT
ATCCGTCATCTTTTGAATGTCGGTGAAATTCTCGGACTGAAGCTTTGCACCATGCAGAACATCTCCTTCTACCTCTGGCT
CACGACGACTGCACGCGAACAGATTCAGAACGGCACCTTCACCGAATGGAAAAACGACTTTCTCAGCAGATTCAACAGCA
ATGAAAAAACATAA

Upstream 100 bases:

>100_bases
GCAGTTTTTTTTTCTGCCTCTGTTTACCTACCTTCAGAGGTTCATAATCGGAACGACTGCCTCCCTTTGCAATGCGGCAA
TACCTCTCTAATCAGGATAG

Downstream 100 bases:

>100_bases
CGTCTTTCTGCTAAGCCTCGGATGCTCAAAAAACACTGTAGACTCCGAACGCCTCATGGCACAGGCGGAGGCATCCGGCA
TAACGTTTACCGAAGAGGCC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MNFTLTATDTKSSARCGVLETSHGTIPTPIFMPVGTRASVKSLEPHELTELGVSIILANTYHLYLRPGNDILRKAGGVHR
FMNWNGPLLTDSGGYQVYSLSDLRKITEEGVTFRSHLDGSKQQFTPENVIDTERIIGSDIIMPLDECPPSTADREYVQTS
GELTIRWAERAKKRFVSTSPLYGHSQFLFGITQGGIHADLRTISTKALVDLDFDGYAIGGMAVGEPAEAMYRVLELSDML
LPARKPRYLMGVGTPENILNAIERGVDMFDCVIPTREGRNGRVYTRNGTMNLRAAKYAADFRPLDEGFDNEVCRNFSRAY
IRHLLNVGEILGLKLCTMQNISFYLWLTTTAREQIQNGTFTEWKNDFLSRFNSNEKT

Sequences:

>Translated_377_residues
MNFTLTATDTKSSARCGVLETSHGTIPTPIFMPVGTRASVKSLEPHELTELGVSIILANTYHLYLRPGNDILRKAGGVHR
FMNWNGPLLTDSGGYQVYSLSDLRKITEEGVTFRSHLDGSKQQFTPENVIDTERIIGSDIIMPLDECPPSTADREYVQTS
GELTIRWAERAKKRFVSTSPLYGHSQFLFGITQGGIHADLRTISTKALVDLDFDGYAIGGMAVGEPAEAMYRVLELSDML
LPARKPRYLMGVGTPENILNAIERGVDMFDCVIPTREGRNGRVYTRNGTMNLRAAKYAADFRPLDEGFDNEVCRNFSRAY
IRHLLNVGEILGLKLCTMQNISFYLWLTTTAREQIQNGTFTEWKNDFLSRFNSNEKT
>Mature_377_residues
MNFTLTATDTKSSARCGVLETSHGTIPTPIFMPVGTRASVKSLEPHELTELGVSIILANTYHLYLRPGNDILRKAGGVHR
FMNWNGPLLTDSGGYQVYSLSDLRKITEEGVTFRSHLDGSKQQFTPENVIDTERIIGSDIIMPLDECPPSTADREYVQTS
GELTIRWAERAKKRFVSTSPLYGHSQFLFGITQGGIHADLRTISTKALVDLDFDGYAIGGMAVGEPAEAMYRVLELSDML
LPARKPRYLMGVGTPENILNAIERGVDMFDCVIPTREGRNGRVYTRNGTMNLRAAKYAADFRPLDEGFDNEVCRNFSRAY
IRHLLNVGEILGLKLCTMQNISFYLWLTTTAREQIQNGTFTEWKNDFLSRFNSNEKT

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=367, Percent_Identity=41.1444141689373, Blast_Score=283, Evalue=3e-76,
Organism=Escherichia coli, GI1786607, Length=370, Percent_Identity=48.3783783783784, Blast_Score=371, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17544680, Length=356, Percent_Identity=37.6404494382022, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI19920510, Length=375, Percent_Identity=38.9333333333333, Blast_Score=291, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42257; Mature: 42257

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFTLTATDTKSSARCGVLETSHGTIPTPIFMPVGTRASVKSLEPHELTELGVSIILANT
CCEEEEECCCCCCCCCCEEECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHCEEEEEEEE
YHLYLRPGNDILRKAGGVHRFMNWNGPLLTDSGGYQVYSLSDLRKITEEGVTFRSHLDGS
EEEEEECCHHHHHHCCCCEEEECCCCCEEECCCCEEEEEHHHHHHHHHHCCEEEHHCCCC
KQQFTPENVIDTERIIGSDIIMPLDECPPSTADREYVQTSGELTIRWAERAKKRFVSTSP
CCCCCCCHHCCHHHHHCCCCEEEHHCCCCCCCCHHHHHCCCCEEEEEHHHHHHHHCCCCC
LYGHSQFLFGITQGGIHADLRTISTKALVDLDFDGYAIGGMAVGEPAEAMYRVLELSDML
CCCCCEEEEEEECCCCHHHHHHCCEEEEEEECCCCEEECCEECCCHHHHHHHHHHHHHHH
LPARKPRYLMGVGTPENILNAIERGVDMFDCVIPTREGRNGRVYTRNGTMNLRAAKYAAD
CCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCEEEEHHHHHHC
FRPLDEGFDNEVCRNFSRAYIRHLLNVGEILGLKLCTMQNISFYLWLTTTAREQIQNGTF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEEEHHHHHHCCCCC
TEWKNDFLSRFNSNEKT
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNFTLTATDTKSSARCGVLETSHGTIPTPIFMPVGTRASVKSLEPHELTELGVSIILANT
CCEEEEECCCCCCCCCCEEECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHCEEEEEEEE
YHLYLRPGNDILRKAGGVHRFMNWNGPLLTDSGGYQVYSLSDLRKITEEGVTFRSHLDGS
EEEEEECCHHHHHHCCCCEEEECCCCCEEECCCCEEEEEHHHHHHHHHHCCEEEHHCCCC
KQQFTPENVIDTERIIGSDIIMPLDECPPSTADREYVQTSGELTIRWAERAKKRFVSTSP
CCCCCCCHHCCHHHHHCCCCEEEHHCCCCCCCCHHHHHCCCCEEEEEHHHHHHHHCCCCC
LYGHSQFLFGITQGGIHADLRTISTKALVDLDFDGYAIGGMAVGEPAEAMYRVLELSDML
CCCCCEEEEEEECCCCHHHHHHCCEEEEEEECCCCEEECCEECCCHHHHHHHHHHHHHHH
LPARKPRYLMGVGTPENILNAIERGVDMFDCVIPTREGRNGRVYTRNGTMNLRAAKYAAD
CCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCEEEEHHHHHHC
FRPLDEGFDNEVCRNFSRAYIRHLLNVGEILGLKLCTMQNISFYLWLTTTAREQIQNGTF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEEEHHHHHHCCCCC
TEWKNDFLSRFNSNEKT
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA