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The map label for this gene is dfrA [H]

Identifier: 119357150

GI number: 119357150

Start: 1533800

End: 1534795

Strand: Reverse

Name: dfrA [H]

Synonym: Cpha266_1339

Alternate gene names: 119357150

Gene position: 1534795-1533800 (Counterclockwise)

Preceding gene: 119357151

Following gene: 119357145

Centisome position: 48.97

GC content: 47.79

Gene sequence:

>996_bases
GTGACCAGGAAAATTCTTGTAACCGGTGGAACCGGCTTTATTGGATCTCGTCTTGTACAGAAACTTGCCGAAACTCCGGA
CGAGGTTTATGTGCTGGTTAGAAAGAGCTCCGATCTTTCCTCACTTTCCGAGGTTCTCGATCACGTGAAGCTTATTTATG
GCGATGTTACCGATCCGGATTCTGTTCATAACGCCATGCAGGGTATCGATTTTGTTTATCACACGGCCGGACTTACCTAT
ATGGGTGACAAGAAGAACGCTCTGCTGAACAAAATCAATGTTGAGGGAACCCGGAATATGCTTGCGGCATCGCTTTCTGC
GGGCGTAAAGCGATTTGTTCATGTCAGTTCAATCACGGCCGTCGGAGTTGCTTATGACCGCAAGCCGCTTAATGAGGCAT
CGACATGGAACTTTGATCGCTTGAATCTTGAATATGCGAGAACAAAACGTCAGGCGGAAGTCGATGTTGCTGAGGCTGTG
AAAAATGGGCTTGATTGCGTTATCGTTAATCCGGCATTTGTTTTTGGGGCTGGTGATATCAATTTTAATGCCGGTCGGCT
CATCAAGGATGTGTATAACAGAAAACTCCCGTTTTATCCGCTTGGAGGGATCTGCGTCGTCGATGTTGAAATAGTCGCTG
AAACGATCATGGCGGCGATGCAGAAGGGAAGAACCGGAGAACGGTATATTATCGGGGGGGACAATGTGTCGTACAAGGAG
CTCGCCGATACCATCTCATCGGTTACCGGCGTTCCCAGGGTTAACTTTCCTCTGCCGTTCTGGATGGCGAAAATTCTGAA
ACAGTTTCTTGCTTTGAAAAAGAGAAAGAACGGTATCTCAAAGCTTTTTAATCTTTCGATGTTCAGGGTGGCTTCAGAGT
TTCTTTACTACGATTCAGCAAAGGCTGTGCGTGAGCTCGGCATGCGCAAAGAGCCCCATGCGAACAGTATCAGGAGCGCG
TTCGAATGGTATCGCGACAGAGATTTGCTGCGTTGA

Upstream 100 bases:

>100_bases
TCCTGAATGGTGAAATTGTTCTGAAATAAAGCCTTATGGCACCAGTACCTCTCCCTTATAACAGGTTTATGGCTCTTTCA
ATATCTGAAGTATATTATTT

Downstream 100 bases:

>100_bases
AAGGAAGCTTTTCTGCTGATCGCAGACTTACGGTTTTCGGGCAAAGAGTATCATGCGGCCGGACTCGTTTTCGTCAAAGG
GTTCGCCTTCATACCCCCCT

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTRKILVTGGTGFIGSRLVQKLAETPDEVYVLVRKSSDLSSLSEVLDHVKLIYGDVTDPDSVHNAMQGIDFVYHTAGLTY
MGDKKNALLNKINVEGTRNMLAASLSAGVKRFVHVSSITAVGVAYDRKPLNEASTWNFDRLNLEYARTKRQAEVDVAEAV
KNGLDCVIVNPAFVFGAGDINFNAGRLIKDVYNRKLPFYPLGGICVVDVEIVAETIMAAMQKGRTGERYIIGGDNVSYKE
LADTISSVTGVPRVNFPLPFWMAKILKQFLALKKRKNGISKLFNLSMFRVASEFLYYDSAKAVRELGMRKEPHANSIRSA
FEWYRDRDLLR

Sequences:

>Translated_331_residues
MTRKILVTGGTGFIGSRLVQKLAETPDEVYVLVRKSSDLSSLSEVLDHVKLIYGDVTDPDSVHNAMQGIDFVYHTAGLTY
MGDKKNALLNKINVEGTRNMLAASLSAGVKRFVHVSSITAVGVAYDRKPLNEASTWNFDRLNLEYARTKRQAEVDVAEAV
KNGLDCVIVNPAFVFGAGDINFNAGRLIKDVYNRKLPFYPLGGICVVDVEIVAETIMAAMQKGRTGERYIIGGDNVSYKE
LADTISSVTGVPRVNFPLPFWMAKILKQFLALKKRKNGISKLFNLSMFRVASEFLYYDSAKAVRELGMRKEPHANSIRSA
FEWYRDRDLLR
>Mature_330_residues
TRKILVTGGTGFIGSRLVQKLAETPDEVYVLVRKSSDLSSLSEVLDHVKLIYGDVTDPDSVHNAMQGIDFVYHTAGLTYM
GDKKNALLNKINVEGTRNMLAASLSAGVKRFVHVSSITAVGVAYDRKPLNEASTWNFDRLNLEYARTKRQAEVDVAEAVK
NGLDCVIVNPAFVFGAGDINFNAGRLIKDVYNRKLPFYPLGGICVVDVEIVAETIMAAMQKGRTGERYIIGGDNVSYKEL
ADTISSVTGVPRVNFPLPFWMAKILKQFLALKKRKNGISKLFNLSMFRVASEFLYYDSAKAVRELGMRKEPHANSIRSAF
EWYRDRDLLR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI310132178, Length=189, Percent_Identity=28.042328042328, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI310113012, Length=189, Percent_Identity=28.042328042328, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI4504507, Length=345, Percent_Identity=25.2173913043478, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI116268111, Length=357, Percent_Identity=26.3305322128852, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI239745448, Length=189, Percent_Identity=28.042328042328, Blast_Score=66, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71987463, Length=331, Percent_Identity=25.9818731117825, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6321437, Length=186, Percent_Identity=30.1075268817204, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 36917; Mature: 36786

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKILVTGGTGFIGSRLVQKLAETPDEVYVLVRKSSDLSSLSEVLDHVKLIYGDVTDPD
CCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHH
SVHNAMQGIDFVYHTAGLTYMGDKKNALLNKINVEGTRNMLAASLSAGVKRFVHVSSITA
HHHHHHHHHHHHHHHCCCEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VGVAYDRKPLNEASTWNFDRLNLEYARTKRQAEVDVAEAVKNGLDCVIVNPAFVFGAGDI
HHEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCC
NFNAGRLIKDVYNRKLPFYPLGGICVVDVEIVAETIMAAMQKGRTGERYIIGGDNVSYKE
CCCHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHH
LADTISSVTGVPRVNFPLPFWMAKILKQFLALKKRKNGISKLFNLSMFRVASEFLYYDSA
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
KAVRELGMRKEPHANSIRSAFEWYRDRDLLR
HHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRKILVTGGTGFIGSRLVQKLAETPDEVYVLVRKSSDLSSLSEVLDHVKLIYGDVTDPD
CCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHH
SVHNAMQGIDFVYHTAGLTYMGDKKNALLNKINVEGTRNMLAASLSAGVKRFVHVSSITA
HHHHHHHHHHHHHHHCCCEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VGVAYDRKPLNEASTWNFDRLNLEYARTKRQAEVDVAEAVKNGLDCVIVNPAFVFGAGDI
HHEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCC
NFNAGRLIKDVYNRKLPFYPLGGICVVDVEIVAETIMAAMQKGRTGERYIIGGDNVSYKE
CCCHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHH
LADTISSVTGVPRVNFPLPFWMAKILKQFLALKKRKNGISKLFNLSMFRVASEFLYYDSA
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
KAVRELGMRKEPHANSIRSAFEWYRDRDLLR
HHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]