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The map label for this gene is pleD [H]

Identifier: 119357129

GI number: 119357129

Start: 1509952

End: 1511226

Strand: Reverse

Name: pleD [H]

Synonym: Cpha266_1318

Alternate gene names: 119357129

Gene position: 1511226-1509952 (Counterclockwise)

Preceding gene: 119357130

Following gene: 119357128

Centisome position: 48.22

GC content: 47.61

Gene sequence:

>1275_bases
ATGAGCAATCAATACAGAAATACGTTACGCCTGCTTCTGCCTGTTTTTCTGACAGCAGGTTTTATTACCGTGACGCTTGT
CGCAGGGACGGCTTTTTTTATGATTTGGCGTCAAACGCTGCTTGACGACCTTAAAGCACGAGAGGCTCTCTGTTTCGATC
TCCTTGATCTCAAGGGGCAGCTTTCTGAAAATCCCCTGCGTTCAGACAGGGATTCGACGATCCATTCGCCGCAACAGAGG
AGCGAAGAGAACTTGCAGATTGTCCGGTTTATGATGAGAAAGGCTGCCGATAATGCCGCAAAGGCGGGTGCCGGAGAGAT
TCGTGCGATTCTGTCAGGAACGTTGAAAGGTGATCAGCTTCTTGAACGGAAGAATATCACGCTGCTCCGACTGGAGCTTG
AGAACATGATCAACAAAACAAAAGAGAGCGTTCGCGAAAGACGGGAGTATTTCGTTTTTGCCAACAATCTTTTTCTCTTT
TTTACATTTGTCGTGCTGGTTGGCTTATGCCTCTGGGAGGGTTTATTGCTGATCCGCCATTACAGGGTGCAATCCGCTCC
GCTTTTTCAGCTTTCGGGGAGGCTCGCGGCGTTTAACAGAAACCTCCCGGAGAGTATTCAGGAAACTGCCGACAAGATTA
AAACGGATATGAAGTTTATGCCGCACTCTTTTGAGATAGGCCAGATAACGGAAACGCTTGTATCTCTTTGCGAGGATGTT
GCGGTAAAAAGCAAAAAACTTGACGAGCTGTTTATTCGGGATGAAAAAACCACCCTCTACAACTACAGGTATTTCAAGGA
ACATCTCATTATTGACGTTGAAAGAGCAAAGCGTTTTGATTACAAGGTCTCTCTTGCCATGATTGATATCGATCATTTCA
AACAGTACAATGATGCCAATGGGCATATAGCCGGAGATCAGGTTCTCGGGCGTCTGGCCGATGTGATCGTCAGCCAGTGT
CGAAGTTCCGATGTTCCTTCGCGCTTTGGCGGCGAAGAGTTTGCCGTTCTTTTTCCCAAAACCGCACACGAGACCGCCAG
CTCTATTGCTGAACGGCTGCGTATGGTTATTTGTGCAGAACCGATACCTCATGAAAAACATCAGCCATCAGGCCGTTTGA
CGGTCAGTATCGGCATTGCAACATTTCCGGACGATGCCGCCGACTGGTATACCCTTATCAATAATGCCGATCGCGCGCTC
TATCACGCAAAAGCCGCCGGAAGAAACATGGTTATCGCATTTTCATCTATTGATCAGGGGGATAAAACGACATGA

Upstream 100 bases:

>100_bases
TTCGGATCCTGGCTTTCGAGAGAGGGGCAGAAAAGTTTTGAGAGAAGTTATCTTGCTCCGTTCCGTCAACCGCCGAGAAC
AATTATTCTGAACTGATGCC

Downstream 100 bases:

>100_bases
GGACAGCGTTTTTTATTGCCAGACGTTTTGCATTCAGACAGCGTTCCGGCTCAAAACCGACGTTTATTGTTCTTGCTTCC
GTTATCGGTATTGCCGTCGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MSNQYRNTLRLLLPVFLTAGFITVTLVAGTAFFMIWRQTLLDDLKAREALCFDLLDLKGQLSENPLRSDRDSTIHSPQQR
SEENLQIVRFMMRKAADNAAKAGAGEIRAILSGTLKGDQLLERKNITLLRLELENMINKTKESVRERREYFVFANNLFLF
FTFVVLVGLCLWEGLLLIRHYRVQSAPLFQLSGRLAAFNRNLPESIQETADKIKTDMKFMPHSFEIGQITETLVSLCEDV
AVKSKKLDELFIRDEKTTLYNYRYFKEHLIIDVERAKRFDYKVSLAMIDIDHFKQYNDANGHIAGDQVLGRLADVIVSQC
RSSDVPSRFGGEEFAVLFPKTAHETASSIAERLRMVICAEPIPHEKHQPSGRLTVSIGIATFPDDAADWYTLINNADRAL
YHAKAAGRNMVIAFSSIDQGDKTT

Sequences:

>Translated_424_residues
MSNQYRNTLRLLLPVFLTAGFITVTLVAGTAFFMIWRQTLLDDLKAREALCFDLLDLKGQLSENPLRSDRDSTIHSPQQR
SEENLQIVRFMMRKAADNAAKAGAGEIRAILSGTLKGDQLLERKNITLLRLELENMINKTKESVRERREYFVFANNLFLF
FTFVVLVGLCLWEGLLLIRHYRVQSAPLFQLSGRLAAFNRNLPESIQETADKIKTDMKFMPHSFEIGQITETLVSLCEDV
AVKSKKLDELFIRDEKTTLYNYRYFKEHLIIDVERAKRFDYKVSLAMIDIDHFKQYNDANGHIAGDQVLGRLADVIVSQC
RSSDVPSRFGGEEFAVLFPKTAHETASSIAERLRMVICAEPIPHEKHQPSGRLTVSIGIATFPDDAADWYTLINNADRAL
YHAKAAGRNMVIAFSSIDQGDKTT
>Mature_423_residues
SNQYRNTLRLLLPVFLTAGFITVTLVAGTAFFMIWRQTLLDDLKAREALCFDLLDLKGQLSENPLRSDRDSTIHSPQQRS
EENLQIVRFMMRKAADNAAKAGAGEIRAILSGTLKGDQLLERKNITLLRLELENMINKTKESVRERREYFVFANNLFLFF
TFVVLVGLCLWEGLLLIRHYRVQSAPLFQLSGRLAAFNRNLPESIQETADKIKTDMKFMPHSFEIGQITETLVSLCEDVA
VKSKKLDELFIRDEKTTLYNYRYFKEHLIIDVERAKRFDYKVSLAMIDIDHFKQYNDANGHIAGDQVLGRLADVIVSQCR
SSDVPSRFGGEEFAVLFPKTAHETASSIAERLRMVICAEPIPHEKHQPSGRLTVSIGIATFPDDAADWYTLINNADRALY
HAKAAGRNMVIAFSSIDQGDKTT

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=243, Percent_Identity=28.8065843621399, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=34.1176470588235, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=36.6459627329193, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI87081881, Length=137, Percent_Identity=43.7956204379562, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI145693134, Length=186, Percent_Identity=32.258064516129, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788381, Length=184, Percent_Identity=33.1521739130435, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=32.7272727272727, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787541, Length=134, Percent_Identity=33.5820895522388, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787816, Length=126, Percent_Identity=35.7142857142857, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787802, Length=131, Percent_Identity=35.8778625954198, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 48221; Mature: 48090

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQYRNTLRLLLPVFLTAGFITVTLVAGTAFFMIWRQTLLDDLKAREALCFDLLDLKGQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSENPLRSDRDSTIHSPQQRSEENLQIVRFMMRKAADNAAKAGAGEIRAILSGTLKGDQL
CCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHCCCCHHHH
LERKNITLLRLELENMINKTKESVRERREYFVFANNLFLFFTFVVLVGLCLWEGLLLIRH
HHHCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
YRVQSAPLFQLSGRLAAFNRNLPESIQETADKIKTDMKFMPHSFEIGQITETLVSLCEDV
HHCCCCCHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AVKSKKLDELFIRDEKTTLYNYRYFKEHLIIDVERAKRFDYKVSLAMIDIDHFKQYNDAN
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHEEEHHHHHCCCEEEEEEEEEHHHHHHCCCCC
GHIAGDQVLGRLADVIVSQCRSSDVPSRFGGEEFAVLFPKTAHETASSIAERLRMVICAE
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCC
PIPHEKHQPSGRLTVSIGIATFPDDAADWYTLINNADRALYHAKAAGRNMVIAFSSIDQG
CCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECCCCC
DKTT
CCCC
>Mature Secondary Structure 
SNQYRNTLRLLLPVFLTAGFITVTLVAGTAFFMIWRQTLLDDLKAREALCFDLLDLKGQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSENPLRSDRDSTIHSPQQRSEENLQIVRFMMRKAADNAAKAGAGEIRAILSGTLKGDQL
CCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHCCCCHHHH
LERKNITLLRLELENMINKTKESVRERREYFVFANNLFLFFTFVVLVGLCLWEGLLLIRH
HHHCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
YRVQSAPLFQLSGRLAAFNRNLPESIQETADKIKTDMKFMPHSFEIGQITETLVSLCEDV
HHCCCCCHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AVKSKKLDELFIRDEKTTLYNYRYFKEHLIIDVERAKRFDYKVSLAMIDIDHFKQYNDAN
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHEEEHHHHHCCCEEEEEEEEEHHHHHHCCCCC
GHIAGDQVLGRLADVIVSQCRSSDVPSRFGGEEFAVLFPKTAHETASSIAERLRMVICAE
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCC
PIPHEKHQPSGRLTVSIGIATFPDDAADWYTLINNADRALYHAKAAGRNMVIAFSSIDQG
CCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECCCCC
DKTT
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA