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The map label for this gene is gph2 [H]

Identifier: 119357050

GI number: 119357050

Start: 1405057

End: 1405746

Strand: Reverse

Name: gph2 [H]

Synonym: Cpha266_1237

Alternate gene names: 119357050

Gene position: 1405746-1405057 (Counterclockwise)

Preceding gene: 119357051

Following gene: 119357045

Centisome position: 44.86

GC content: 49.42

Gene sequence:

>690_bases
ATGCCCTGTAAAGCAGTAATCTTTGATCTTGACGGAACCCTTCTCAATACTCTTGAAGATATAGTCAACACCCTCAACTC
CGTTCTTTATCAGCATAACTATCCACAGCACTCGGTTGATGCCTGCCGGTTTCTTGTTGGCCACGGCATGAGAGAACTTG
TCAAAAAAGCCCTTCCCGAAGAGGCCGGAACACCGGAAATTATCGATTCCATGCTTGAGGATCTGCTGATCCATTATGCT
GAAAACTGGAATGTGCACTCCCGCCCCTATGAAGGTATTGCCGCAATGCTTGACGAACTGACCGCACAGGGAATAAAAAA
AGCCATTCTCTCCAATAAAGCCGATAATTTCACCCGTCTGTGTGCTGAACAATTGCTTTCAGATTGGCATTTCGATATGG
TCATGGGTCATACCGATACGTTTACGCACAAGCCGGATCCTTCAGGAGCGCTGTTTATAGCCGGTCAGCTCGGTATCGAA
CCCGGTGATATTCTCTATGTCGGAGACAGCGGGATCGATATGCTGACGGCTACCCGAGCCGGAATGTTCCCGCTCGGAGT
ATTGTGGGGGTTCAGACCTGAATCCGAACTGCTGCAATTCGGAGCAAAAGCGCTCGTAAAAGAACCGGGAGAGATCATCA
CGATGCTTATGAACTGTCCGGGCTGTCCGGGAAATTCAGCATCGCGCTGA

Upstream 100 bases:

>100_bases
GGACGGGAATAACGAAACCTTATTCAATATTTCTTGTTACGGCCCATAAGCCGCTATTGTAAAGCCACTATTATGAACAA
ACTGATTAATCACTCCATTT

Downstream 100 bases:

>100_bases
AAGGGTACTGCTCAAGAAAAGGAAAAAACGAACGCCGGCTTCGTCTCTCCGGCTGTAAAGAGCCGAGCCAGTCCTGTTCC
GCCACGCAACTATCTCCCAT

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP 2; PGPase2 [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MPCKAVIFDLDGTLLNTLEDIVNTLNSVLYQHNYPQHSVDACRFLVGHGMRELVKKALPEEAGTPEIIDSMLEDLLIHYA
ENWNVHSRPYEGIAAMLDELTAQGIKKAILSNKADNFTRLCAEQLLSDWHFDMVMGHTDTFTHKPDPSGALFIAGQLGIE
PGDILYVGDSGIDMLTATRAGMFPLGVLWGFRPESELLQFGAKALVKEPGEIITMLMNCPGCPGNSASR

Sequences:

>Translated_229_residues
MPCKAVIFDLDGTLLNTLEDIVNTLNSVLYQHNYPQHSVDACRFLVGHGMRELVKKALPEEAGTPEIIDSMLEDLLIHYA
ENWNVHSRPYEGIAAMLDELTAQGIKKAILSNKADNFTRLCAEQLLSDWHFDMVMGHTDTFTHKPDPSGALFIAGQLGIE
PGDILYVGDSGIDMLTATRAGMFPLGVLWGFRPESELLQFGAKALVKEPGEIITMLMNCPGCPGNSASR
>Mature_228_residues
PCKAVIFDLDGTLLNTLEDIVNTLNSVLYQHNYPQHSVDACRFLVGHGMRELVKKALPEEAGTPEIIDSMLEDLLIHYAE
NWNVHSRPYEGIAAMLDELTAQGIKKAILSNKADNFTRLCAEQLLSDWHFDMVMGHTDTFTHKPDPSGALFIAGQLGIEP
GDILYVGDSGIDMLTATRAGMFPLGVLWGFRPESELLQFGAKALVKEPGEIITMLMNCPGCPGNSASR

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=208, Percent_Identity=25.9615384615385, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 25159; Mature: 25028

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCKAVIFDLDGTLLNTLEDIVNTLNSVLYQHNYPQHSVDACRFLVGHGMRELVKKALPE
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
EAGTPEIIDSMLEDLLIHYAENWNVHSRPYEGIAAMLDELTAQGIKKAILSNKADNFTRL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
CAEQLLSDWHFDMVMGHTDTFTHKPDPSGALFIAGQLGIEPGDILYVGDSGIDMLTATRA
HHHHHHHHHCHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHC
GMFPLGVLWGFRPESELLQFGAKALVKEPGEIITMLMNCPGCPGNSASR
CCCHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PCKAVIFDLDGTLLNTLEDIVNTLNSVLYQHNYPQHSVDACRFLVGHGMRELVKKALPE
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
EAGTPEIIDSMLEDLLIHYAENWNVHSRPYEGIAAMLDELTAQGIKKAILSNKADNFTRL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
CAEQLLSDWHFDMVMGHTDTFTHKPDPSGALFIAGQLGIEPGDILYVGDSGIDMLTATRA
HHHHHHHHHCHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHC
GMFPLGVLWGFRPESELLQFGAKALVKEPGEIITMLMNCPGCPGNSASR
CCCHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]