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The map label for this gene is folD [H]

Identifier: 119356824

GI number: 119356824

Start: 1135922

End: 1136848

Strand: Reverse

Name: folD [H]

Synonym: Cpha266_0997

Alternate gene names: 119356824

Gene position: 1136848-1135922 (Counterclockwise)

Preceding gene: 119356833

Following gene: 119356823

Centisome position: 36.28

GC content: 50.38

Gene sequence:

>927_bases
ATGGAGTGTGGCATCTCTATAACCATGAAAAAGACCATGATGCAGATTATTGACGGTAAAAAAATTTCTCAGGATCTGAA
AAATGAACTGAAAACCGATGTAGCCCACTTTCAGGCGAGCATCGGAAAAGTTCCCGGCCTGACGGTCATTATTGTTGGAC
ACGACCCCGCCTCACAGGTTTACGTGCGCAACAAGGCTAAAAGCTGCCTTGAAATTGGCATGCACTCAACCGTCATCGAA
ATGCCGCACGACACCCCGCAGGAGACGCTGCTCCAGGCCATCAGAGAACTGAACCAGGATCCGGCCGTACACGGCATTCT
GGTTCAGCAGCCGCTGCCGAAACAGATCGACGAGTTTGCCGTCACCCTTGCCATTGACCCATCCAAAGATGTTGACGGGT
TTCATCCGGAAAACCTTGGCCGCCTTGTCATGGGCCATCTTGATAAATGTTTTGTCAGTTGCACTCCGTATGGCATTCTC
GAACTGCTTGACCGCTACAACATCGAAACCAAAGGAAAACACTGCGTGGTTGTCGGACGAAGCAATATTGTCGGAAAACC
CATGGCAAACCTGATGATGCAGAAACTCGATGCAACCAACTGCACTGTTACCGTCTGCCATTCGGCAACCAGAGATATTC
CATCTTTTACAAAACAGGCCGACATTCTCATAGCCGCTCTCGGCAAAGCCCGCTTTATTACGGCAGATATGGTCAAACCC
GGCGCAGTGGTGATTGATGTAGGGATCAACCGCATTGAAGATCCTTCAACAAAAAGCGGTTACCGTCTGGTTGGAGATGT
TGACTATGATGGAGTTGCGGCACTTGCGTCAGCCATAACACCCGTTCCGGGCGGAGTCGGGCCTATGACCATTGCGATGC
TGTTGAAAAACACACTCCAGTCGTTCCGCCGCATCAACAACCTTTAG

Upstream 100 bases:

>100_bases
TAAAAAACCTAACTTAACAAAAATCAGGCAAATGCAGCGGGCGGAACAAAAGCTGTTGGAGTAAAATGAAAAATCGCGAT
ATTGGTTTTAACCCTTACCA

Downstream 100 bases:

>100_bases
GAGGCCATGTCCCGTCCAGCAACACGGTATCAATGCACGCAATGCGGCGCAGTGACCCTTAAATATCAGGGGAAATGCTT
CGCCTGCCAGAGTTGGGGAA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MECGISITMKKTMMQIIDGKKISQDLKNELKTDVAHFQASIGKVPGLTVIIVGHDPASQVYVRNKAKSCLEIGMHSTVIE
MPHDTPQETLLQAIRELNQDPAVHGILVQQPLPKQIDEFAVTLAIDPSKDVDGFHPENLGRLVMGHLDKCFVSCTPYGIL
ELLDRYNIETKGKHCVVVGRSNIVGKPMANLMMQKLDATNCTVTVCHSATRDIPSFTKQADILIAALGKARFITADMVKP
GAVVIDVGINRIEDPSTKSGYRLVGDVDYDGVAALASAITPVPGGVGPMTIAMLLKNTLQSFRRINNL

Sequences:

>Translated_308_residues
MECGISITMKKTMMQIIDGKKISQDLKNELKTDVAHFQASIGKVPGLTVIIVGHDPASQVYVRNKAKSCLEIGMHSTVIE
MPHDTPQETLLQAIRELNQDPAVHGILVQQPLPKQIDEFAVTLAIDPSKDVDGFHPENLGRLVMGHLDKCFVSCTPYGIL
ELLDRYNIETKGKHCVVVGRSNIVGKPMANLMMQKLDATNCTVTVCHSATRDIPSFTKQADILIAALGKARFITADMVKP
GAVVIDVGINRIEDPSTKSGYRLVGDVDYDGVAALASAITPVPGGVGPMTIAMLLKNTLQSFRRINNL
>Mature_308_residues
MECGISITMKKTMMQIIDGKKISQDLKNELKTDVAHFQASIGKVPGLTVIIVGHDPASQVYVRNKAKSCLEIGMHSTVIE
MPHDTPQETLLQAIRELNQDPAVHGILVQQPLPKQIDEFAVTLAIDPSKDVDGFHPENLGRLVMGHLDKCFVSCTPYGIL
ELLDRYNIETKGKHCVVVGRSNIVGKPMANLMMQKLDATNCTVTVCHSATRDIPSFTKQADILIAALGKARFITADMVKP
GAVVIDVGINRIEDPSTKSGYRLVGDVDYDGVAALASAITPVPGGVGPMTIAMLLKNTLQSFRRINNL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=294, Percent_Identity=42.5170068027211, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI94721354, Length=299, Percent_Identity=45.1505016722408, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI222418558, Length=296, Percent_Identity=44.2567567567568, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI36796743, Length=202, Percent_Identity=30.1980198019802, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1786741, Length=287, Percent_Identity=48.0836236933798, Blast_Score=253, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17568735, Length=263, Percent_Identity=42.5855513307985, Blast_Score=198, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6319558, Length=301, Percent_Identity=43.5215946843854, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=42.1404682274247, Blast_Score=243, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6322933, Length=327, Percent_Identity=26.9113149847095, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17136816, Length=298, Percent_Identity=48.3221476510067, Blast_Score=263, Evalue=8e-71,
Organism=Drosophila melanogaster, GI17136818, Length=298, Percent_Identity=48.3221476510067, Blast_Score=263, Evalue=9e-71,
Organism=Drosophila melanogaster, GI62472483, Length=300, Percent_Identity=45.6666666666667, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI45551871, Length=300, Percent_Identity=45.6666666666667, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24645718, Length=294, Percent_Identity=46.2585034013605, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI17137370, Length=294, Percent_Identity=46.2585034013605, Blast_Score=255, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 33437; Mature: 33437

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MECGISITMKKTMMQIIDGKKISQDLKNELKTDVAHFQASIGKVPGLTVIIVGHDPASQV
CCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHH
YVRNKAKSCLEIGMHSTVIEMPHDTPQETLLQAIRELNQDPAVHGILVQQPLPKQIDEFA
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHEE
VTLAIDPSKDVDGFHPENLGRLVMGHLDKCFVSCTPYGILELLDRYNIETKGKHCVVVGR
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEEC
SNIVGKPMANLMMQKLDATNCTVTVCHSATRDIPSFTKQADILIAALGKARFITADMVKP
CCCCCCHHHHHHHHHHCCCCCEEEEECCHHCCCHHHHHHHHHHEEECCCCCEEECCCCCC
GAVVIDVGINRIEDPSTKSGYRLVGDVDYDGVAALASAITPVPGGVGPMTIAMLLKNTLQ
CEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
SFRRINNL
HHHHHCCC
>Mature Secondary Structure
MECGISITMKKTMMQIIDGKKISQDLKNELKTDVAHFQASIGKVPGLTVIIVGHDPASQV
CCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHH
YVRNKAKSCLEIGMHSTVIEMPHDTPQETLLQAIRELNQDPAVHGILVQQPLPKQIDEFA
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHEE
VTLAIDPSKDVDGFHPENLGRLVMGHLDKCFVSCTPYGILELLDRYNIETKGKHCVVVGR
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEEC
SNIVGKPMANLMMQKLDATNCTVTVCHSATRDIPSFTKQADILIAALGKARFITADMVKP
CCCCCCHHHHHHHHHHCCCCCEEEEECCHHCCCHHHHHHHHHHEEECCCCCEEECCCCCC
GAVVIDVGINRIEDPSTKSGYRLVGDVDYDGVAALASAITPVPGGVGPMTIAMLLKNTLQ
CEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
SFRRINNL
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA