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The map label for this gene is leuC [H]

Identifier: 119356679

GI number: 119356679

Start: 970211

End: 971506

Strand: Reverse

Name: leuC [H]

Synonym: Cpha266_0848

Alternate gene names: 119356679

Gene position: 971506-970211 (Counterclockwise)

Preceding gene: 119356680

Following gene: 119356678

Centisome position: 31.0

GC content: 54.4

Gene sequence:

>1296_bases
ATGGCACAAACAATAACCCAGAAAATCCTTTCAAGGGCGGCAAACCGCAAGTTTGTAGATGCCGGGGAAAACGTCTGGCT
GAACGTCGATATCCTGCTCACACACGATGTGTGCGGACCGCCTACCTTCGACATCTTCAAACAGGAATTCGGACCCGACG
CGAAGGTCTGGGATCCTGAAAAAGTGGTTGTGCTACCCGACCACTACATCTTTACCGCTAACGAGCATGCGCACAGAAAT
ATTGACCTCTTGCGGCAGTTTGCCCGTGAACAGAGTCTCCCCAACTACTACGATGTTGGCACAAAACGATATAAAGGGGT
CTGCCATGTCGCACTGGCTGAAGAGGGATTCAACATTCCGGGAACCGTGCTCTTTGGAACCGATTCGCACACCTGCACGT
CGGGAGCCTTCGGCATGTTCGGCTCGGGCATCGGCAATACTGACGCGGCATTCATTCTCGGCACCGGCAAACTCTGGGAA
AAAGTCCCCGAATCCATGAAGTTCACCTTCGATGGCCAGATGCCTGAATACCTCACCGCCAAAGACCTTATCTTGCATAT
TCTCGGCGATATCGGCACCGATGGCGCGACCTACCGGGCCATGGAATTTGACGGCGAAGCGGTCTTCTCTCTTCCGATTG
AAGAGCGAATGACGCTTACCAACATGGCAATCGAGGCTGGCGGCATGAACGGCATCATCGCGGCAGATGCCATTACCGAG
GCATACGTCAACGCAAGAACGCAGAAACCATATGAGCTCTTCAGGAGCGATCCTGATGCCCGTTACCACAGCAGCTACAC
CTACAATGTCCGGGAACTTGAGCCCGTGGTCGCATGTCCGCACAGCCCGGATAACCGTGCAACCGTCAGAAGCGTTCAGG
GAACGGCCATCACCAGATCCTATATCGGCTCATGTACCGGCGGCAAACTCAGTGATTTCATGATGGCGGCTAAAATCCTC
AACGGCAAAAAAGTATCGGTTCCCACCAATATCGTTCCGGCAACCGTCAAGGTAGCCGCTGATCTTGCAATCGAACAGTA
TGAAGGGAGAACCCTGAAGCAGATTTTCGAGGATGCCGGATGCAGCATCGCCCTGCCCTCCTGTGCGGCCTGTCTCGGCG
GTCCGAGCGATACCGTCGGACGTTCGGTTGATAACGACGTCGTTGTGTCAACGACAAACAGGAATTTCCCTGGACGCATG
GGCAGTAAAAAAGCCGGTGTCTACCTTGCCTCACCGTTGACGGCAGCAGCGTCAGCCATTACCGGCAAGCTCACCGACCC
GAGAGATTTCCTCTGA

Upstream 100 bases:

>100_bases
GGCAAGCGCAAAAGCTTACCTCCAGGCACTCAGCCTGAGCCGGACATATTTTGAAACAGACAACACTACAGAAACCATAG
ATAACGGGGTTTAACAAACC

Downstream 100 bases:

>100_bases
TCATGAACTTGTAAGGAAAATAAAATGGAAACCATTATACAGGGAAAAGCCTACGTTTTAGGCAAAAACATCGATACCGA
CCAGATCATACCGGCTGAAC

Product: homoaconitate hydratase family protein

Products: NA

Alternate protein names: (R)-2-methylmalate dehydratase; (R)-citramalate dehydratase; 3-isopropylmalate dehydratase; Alpha-isopropylmalate dehydratase; Citraconate hydratase; Isopropylmalate isomerase; IPMI; Maleate hydratase; Malease [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MAQTITQKILSRAANRKFVDAGENVWLNVDILLTHDVCGPPTFDIFKQEFGPDAKVWDPEKVVVLPDHYIFTANEHAHRN
IDLLRQFAREQSLPNYYDVGTKRYKGVCHVALAEEGFNIPGTVLFGTDSHTCTSGAFGMFGSGIGNTDAAFILGTGKLWE
KVPESMKFTFDGQMPEYLTAKDLILHILGDIGTDGATYRAMEFDGEAVFSLPIEERMTLTNMAIEAGGMNGIIAADAITE
AYVNARTQKPYELFRSDPDARYHSSYTYNVRELEPVVACPHSPDNRATVRSVQGTAITRSYIGSCTGGKLSDFMMAAKIL
NGKKVSVPTNIVPATVKVAADLAIEQYEGRTLKQIFEDAGCSIALPSCAACLGGPSDTVGRSVDNDVVVSTTNRNFPGRM
GSKKAGVYLASPLTAAASAITGKLTDPRDFL

Sequences:

>Translated_431_residues
MAQTITQKILSRAANRKFVDAGENVWLNVDILLTHDVCGPPTFDIFKQEFGPDAKVWDPEKVVVLPDHYIFTANEHAHRN
IDLLRQFAREQSLPNYYDVGTKRYKGVCHVALAEEGFNIPGTVLFGTDSHTCTSGAFGMFGSGIGNTDAAFILGTGKLWE
KVPESMKFTFDGQMPEYLTAKDLILHILGDIGTDGATYRAMEFDGEAVFSLPIEERMTLTNMAIEAGGMNGIIAADAITE
AYVNARTQKPYELFRSDPDARYHSSYTYNVRELEPVVACPHSPDNRATVRSVQGTAITRSYIGSCTGGKLSDFMMAAKIL
NGKKVSVPTNIVPATVKVAADLAIEQYEGRTLKQIFEDAGCSIALPSCAACLGGPSDTVGRSVDNDVVVSTTNRNFPGRM
GSKKAGVYLASPLTAAASAITGKLTDPRDFL
>Mature_430_residues
AQTITQKILSRAANRKFVDAGENVWLNVDILLTHDVCGPPTFDIFKQEFGPDAKVWDPEKVVVLPDHYIFTANEHAHRNI
DLLRQFAREQSLPNYYDVGTKRYKGVCHVALAEEGFNIPGTVLFGTDSHTCTSGAFGMFGSGIGNTDAAFILGTGKLWEK
VPESMKFTFDGQMPEYLTAKDLILHILGDIGTDGATYRAMEFDGEAVFSLPIEERMTLTNMAIEAGGMNGIIAADAITEA
YVNARTQKPYELFRSDPDARYHSSYTYNVRELEPVVACPHSPDNRATVRSVQGTAITRSYIGSCTGGKLSDFMMAAKILN
GKKVSVPTNIVPATVKVAADLAIEQYEGRTLKQIFEDAGCSIALPSCAACLGGPSDTVGRSVDNDVVVSTTNRNFPGRMG
SKKAGVYLASPLTAAASAITGKLTDPRDFL

Specific function: Enzyme with broad specificity that catalyzes reversible hydroxyacid isomerizations via dehydration/hydration reactions. Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate, a step involved

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=374, Percent_Identity=25.668449197861, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1786259, Length=483, Percent_Identity=31.2629399585921, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI2367097, Length=471, Percent_Identity=26.5392781316348, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787531, Length=388, Percent_Identity=23.9690721649485, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI25149337, Length=351, Percent_Identity=27.3504273504274, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI32564738, Length=351, Percent_Identity=27.3504273504274, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17568399, Length=423, Percent_Identity=24.822695035461, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25149342, Length=289, Percent_Identity=26.2975778546713, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=466, Percent_Identity=29.1845493562232, Blast_Score=176, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6320440, Length=467, Percent_Identity=30.8351177730193, Blast_Score=172, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6323335, Length=392, Percent_Identity=26.530612244898, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322261, Length=377, Percent_Identity=25.1989389920424, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28571643, Length=351, Percent_Identity=27.0655270655271, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI161076999, Length=342, Percent_Identity=24.5614035087719, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281365315, Length=342, Percent_Identity=24.5614035087719, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17864292, Length=342, Percent_Identity=24.5614035087719, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137564, Length=367, Percent_Identity=26.4305177111717, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24645686, Length=367, Percent_Identity=23.9782016348774, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33; =4.2.1.35; =4.2.1.31 [H]

Molecular weight: Translated: 46720; Mature: 46589

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTITQKILSRAANRKFVDAGENVWLNVDILLTHDVCGPPTFDIFKQEFGPDAKVWDPE
CCHHHHHHHHHHHHCCCEEECCCCEEEEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCC
KVVVLPDHYIFTANEHAHRNIDLLRQFAREQSLPNYYDVGTKRYKGVCHVALAEEGFNIP
EEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHCCCHHHCCEEEEEEECCCCCCC
GTVLFGTDSHTCTSGAFGMFGSGIGNTDAAFILGTGKLWEKVPESMKFTFDGQMPEYLTA
EEEEECCCCCCCCCCCCHHCCCCCCCCCCEEEEECCHHHHHCCHHEEEEECCCCCCHHHH
KDLILHILGDIGTDGATYRAMEFDGEAVFSLPIEERMTLTNMAIEAGGMNGIIAADAITE
HHHHHHHHHHCCCCCCEEEEEEECCCEEEECCHHHHHHHHHHEEECCCCCCEEEHHHHHH
AYVNARTQKPYELFRSDPDARYHSSYTYNVRELEPVVACPHSPDNRATVRSVQGTAITRS
HHHHCCCCCCHHHHCCCCCCCCCCCEEEEHHHCCEEEECCCCCCCCCEEEECCCCCHHHH
YIGSCTGGKLSDFMMAAKILNGKKVSVPTNIVPATVKVAADLAIEQYEGRTLKQIFEDAG
HHCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCC
CSIALPSCAACLGGPSDTVGRSVDNDVVVSTTNRNFPGRMGSKKAGVYLASPLTAAASAI
CEEECCHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHH
TGKLTDPRDFL
CCCCCCCCCCC
>Mature Secondary Structure 
AQTITQKILSRAANRKFVDAGENVWLNVDILLTHDVCGPPTFDIFKQEFGPDAKVWDPE
CHHHHHHHHHHHHCCCEEECCCCEEEEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCC
KVVVLPDHYIFTANEHAHRNIDLLRQFAREQSLPNYYDVGTKRYKGVCHVALAEEGFNIP
EEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHCCCHHHCCEEEEEEECCCCCCC
GTVLFGTDSHTCTSGAFGMFGSGIGNTDAAFILGTGKLWEKVPESMKFTFDGQMPEYLTA
EEEEECCCCCCCCCCCCHHCCCCCCCCCCEEEEECCHHHHHCCHHEEEEECCCCCCHHHH
KDLILHILGDIGTDGATYRAMEFDGEAVFSLPIEERMTLTNMAIEAGGMNGIIAADAITE
HHHHHHHHHHCCCCCCEEEEEEECCCEEEECCHHHHHHHHHHEEECCCCCCEEEHHHHHH
AYVNARTQKPYELFRSDPDARYHSSYTYNVRELEPVVACPHSPDNRATVRSVQGTAITRS
HHHHCCCCCCHHHHCCCCCCCCCCCEEEEHHHCCEEEECCCCCCCCCEEEECCCCCHHHH
YIGSCTGGKLSDFMMAAKILNGKKVSVPTNIVPATVKVAADLAIEQYEGRTLKQIFEDAG
HHCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCC
CSIALPSCAACLGGPSDTVGRSVDNDVVVSTTNRNFPGRMGSKKAGVYLASPLTAAASAI
CEEECCHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHH
TGKLTDPRDFL
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]