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The map label for this gene is thiH [H]

Identifier: 119356563

GI number: 119356563

Start: 830283

End: 831347

Strand: Reverse

Name: thiH [H]

Synonym: Cpha266_0729

Alternate gene names: 119356563

Gene position: 831347-830283 (Counterclockwise)

Preceding gene: 119356564

Following gene: 119356562

Centisome position: 26.53

GC content: 54.18

Gene sequence:

>1065_bases
ATGAACAGCTTGCCTGAATGGCTGACCAGTGAAGAGAACTCCCGGCAACTTGCCGCAATGCTTGCTCCATCATCATCTTT
CAGTCTTGAAGCCCTTGCTGCTGAATCGAGAGCCGTGACCCTGCGTCGTTTCGGCCGCACCATGACGCTTTATGCGCCCC
TCTACCTTTCGAATTACTGTTCGAGCGGCTGCGCATACTGCGGCTTTGCCTCTGACAGACAAACATCCCGACGACGCCTC
GAGCCAAGGGAGATTGAAAAAGAACTGATCGCCATGAAAAAACTTGGCATTAGCGACGTGCTCCTCCTGACCGGTGAACG
GACCGCATCTGCCGGGTTTGACTATCTGCTGGCTTGCGTCAAAATAGCTGCCGGCCAGATGCAGCGCGTAGCTGTTGAGG
CGTTTCCGATGAGTGTCGAGGAATACCGGGAACTCGCTCTTGGCGGATGCACCGGCATTACGATTTATCAGGAAACCTAC
AACCGTGAAAACTACGAAAAGCTCCACCGCTGGGGCCCTAAAAAAAACTTTTTCGACCGCCTTGAAACTCCTGCCCGGGC
ACTTGAGGCCGGCATTAAAACCGTGGGACTCGGTGTTCTCCTCGGACTGTCCGAACCGGTCGAAGATGCGCTCAGGCTCT
ATCGACACGTACGACACCTCAGCCGAACCTACTGGCGTGCCGGCATTACGGTATCGTTTCCCCGTATGCGGCCGGAACCC
GGTGGCTATACGCCCCCGTTTTCTATTGACGATCACTTTCTTGCCCGTATGATCTTTGCGTTCCGTATTGCGCTTCCCGA
TACGGAACTGGTACTGTCAACCAGGGAAAGCCCTGCATTTCGTGACGGAATGGCCGGGCTGGGAATCACCAGAATGAGCA
TTGAAAGCCGCACCACAGTCGGAGGCTATCATGATACGTCCCACAATGAAAAAGGCCAGTTCGACGTTTATGACAACCGG
ACAGCCAAAGAGTTCTGCAACGCCCTGCAAAAACAAAACATCGAACCGGTTTTCAAAAACTGGGAGCCGGTTTATAACGG
CCCGTCATCTGCTGCATCGGCATGA

Upstream 100 bases:

>100_bases
CCGTAAGAGCCGGACGCAGGGCATTCAAGGCAGAGCTGATGCCGAAAAGCAGCTTCGCTCTTTCGACAAGCCCGCTAACA
ACTTTTCTCGGAAAGAAATC

Downstream 100 bases:

>100_bases
TATTGACTGACGATCAACAACAGCGCTACTCGCGTCATCTCTCTCTTCCGGAAATCGGCGAAAAAGGACAAAAAAAACTG
CTTCGCTCAAAGGTACTTGT

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MNSLPEWLTSEENSRQLAAMLAPSSSFSLEALAAESRAVTLRRFGRTMTLYAPLYLSNYCSSGCAYCGFASDRQTSRRRL
EPREIEKELIAMKKLGISDVLLLTGERTASAGFDYLLACVKIAAGQMQRVAVEAFPMSVEEYRELALGGCTGITIYQETY
NRENYEKLHRWGPKKNFFDRLETPARALEAGIKTVGLGVLLGLSEPVEDALRLYRHVRHLSRTYWRAGITVSFPRMRPEP
GGYTPPFSIDDHFLARMIFAFRIALPDTELVLSTRESPAFRDGMAGLGITRMSIESRTTVGGYHDTSHNEKGQFDVYDNR
TAKEFCNALQKQNIEPVFKNWEPVYNGPSSAASA

Sequences:

>Translated_354_residues
MNSLPEWLTSEENSRQLAAMLAPSSSFSLEALAAESRAVTLRRFGRTMTLYAPLYLSNYCSSGCAYCGFASDRQTSRRRL
EPREIEKELIAMKKLGISDVLLLTGERTASAGFDYLLACVKIAAGQMQRVAVEAFPMSVEEYRELALGGCTGITIYQETY
NRENYEKLHRWGPKKNFFDRLETPARALEAGIKTVGLGVLLGLSEPVEDALRLYRHVRHLSRTYWRAGITVSFPRMRPEP
GGYTPPFSIDDHFLARMIFAFRIALPDTELVLSTRESPAFRDGMAGLGITRMSIESRTTVGGYHDTSHNEKGQFDVYDNR
TAKEFCNALQKQNIEPVFKNWEPVYNGPSSAASA
>Mature_354_residues
MNSLPEWLTSEENSRQLAAMLAPSSSFSLEALAAESRAVTLRRFGRTMTLYAPLYLSNYCSSGCAYCGFASDRQTSRRRL
EPREIEKELIAMKKLGISDVLLLTGERTASAGFDYLLACVKIAAGQMQRVAVEAFPMSVEEYRELALGGCTGITIYQETY
NRENYEKLHRWGPKKNFFDRLETPARALEAGIKTVGLGVLLGLSEPVEDALRLYRHVRHLSRTYWRAGITVSFPRMRPEP
GGYTPPFSIDDHFLARMIFAFRIALPDTELVLSTRESPAFRDGMAGLGITRMSIESRTTVGGYHDTSHNEKGQFDVYDNR
TAKEFCNALQKQNIEPVFKNWEPVYNGPSSAASA

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=327, Percent_Identity=40.6727828746177, Blast_Score=255, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39717; Mature: 39717

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLPEWLTSEENSRQLAAMLAPSSSFSLEALAAESRAVTLRRFGRTMTLYAPLYLSNYC
CCCCCHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHH
SSGCAYCGFASDRQTSRRRLEPREIEKELIAMKKLGISDVLLLTGERTASAGFDYLLACV
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCHHHHHHHH
KIAAGQMQRVAVEAFPMSVEEYRELALGGCTGITIYQETYNRENYEKLHRWGPKKNFFDR
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHCCCCHHHHHH
LETPARALEAGIKTVGLGVLLGLSEPVEDALRLYRHVRHLSRTYWRAGITVSFPRMRPEP
HCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
GGYTPPFSIDDHFLARMIFAFRIALPDTELVLSTRESPAFRDGMAGLGITRMSIESRTTV
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHCCCCCCCEEEEECCCCCCC
GGYHDTSHNEKGQFDVYDNRTAKEFCNALQKQNIEPVFKNWEPVYNGPSSAASA
CCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSLPEWLTSEENSRQLAAMLAPSSSFSLEALAAESRAVTLRRFGRTMTLYAPLYLSNYC
CCCCCHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHH
SSGCAYCGFASDRQTSRRRLEPREIEKELIAMKKLGISDVLLLTGERTASAGFDYLLACV
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCHHHHHHHH
KIAAGQMQRVAVEAFPMSVEEYRELALGGCTGITIYQETYNRENYEKLHRWGPKKNFFDR
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHCCCCHHHHHH
LETPARALEAGIKTVGLGVLLGLSEPVEDALRLYRHVRHLSRTYWRAGITVSFPRMRPEP
HCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
GGYTPPFSIDDHFLARMIFAFRIALPDTELVLSTRESPAFRDGMAGLGITRMSIESRTTV
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHCCCCCCCEEEEECCCCCCC
GGYHDTSHNEKGQFDVYDNRTAKEFCNALQKQNIEPVFKNWEPVYNGPSSAASA
CCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]