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The map label for this gene is thiG

Identifier: 119356564

GI number: 119356564

Start: 831344

End: 832123

Strand: Reverse

Name: thiG

Synonym: Cpha266_0730

Alternate gene names: 119356564

Gene position: 832123-831344 (Counterclockwise)

Preceding gene: 119356565

Following gene: 119356563

Centisome position: 26.55

GC content: 54.1

Gene sequence:

>780_bases
ATGGACTTTTTGCACTTGGGATCCTTTGTTTTTTCTTCCCGCCTGATTCTTGGAACAGGCAAATTCAGCAATGCCGACCT
CATGATTGAGGCAATAAAAGCTTCAGGGGCACAACTTGTCACCGTGGCGCTGCGACGATTCAACCGTGAGCAGATTGCCG
ATGATCTTTTCGGGCCGCTTTCCGCCTTGCAGGGAATTACGCTTATGCCGAACACTTCGGGAGCCTCGACCGCCAGGGAA
GCCATCCATGCGGCCCATATAGCAAGAGAGCTTTCCGGCAGCCCGTTTATCAAGGTAGAGATTCATCCCAACCCGCAGCA
CCTTATGCCCGATGCTCTTGAAACCTGGGAGGCTTCAAGAATTCTCGCAAAGGAAGGATTTCTTGTCATGCCCTACATCC
CTGCAGATCCGGTGCTTGCAAAAAGACTTGAGGAGGTTGGTTGTGCCTCAGTCATGCCGCTCGGTTCGGCAATCGGGAGC
GGTCAGGGGCTGGCAAACGCAGGGATGATCGAACTGATCATCAGGGAGAGCGGCATTCCTGTTATTGTCGATGCAGGACT
CAGAGCTCCTTCCGAAGCGGCGGCAGCCATGGAAATGGGTTGCGGAGCCGTGCTGGTGAACAGTGCCGTAGCCGTTGCCG
GAAACCCTCCTGAAATGGCAAATGCCTTTGCCGAAGCCGTAAGAGCCGGACGCAGGGCATTCAAGGCAGAGCTGATGCCG
AAAAGCAGCTTCGCTCTTTCGACAAGCCCGCTAACAACTTTTCTCGGAAAGAAATCATGA

Upstream 100 bases:

>100_bases
CCCGAAAATCGTTCCTTACATCTTCTCATGGAAGGAGATCGTGTCGAAATTCTGGTTTTTGCAGGCGGAGGGTAAACGAC
GTCAGTAATAAAAAAGAGTT

Downstream 100 bases:

>100_bases
ACAGCTTGCCTGAATGGCTGACCAGTGAAGAGAACTCCCGGCAACTTGCCGCAATGCTTGCTCCATCATCATCTTTCAGT
CTTGAAGCCCTTGCTGCTGA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MDFLHLGSFVFSSRLILGTGKFSNADLMIEAIKASGAQLVTVALRRFNREQIADDLFGPLSALQGITLMPNTSGASTARE
AIHAAHIARELSGSPFIKVEIHPNPQHLMPDALETWEASRILAKEGFLVMPYIPADPVLAKRLEEVGCASVMPLGSAIGS
GQGLANAGMIELIIRESGIPVIVDAGLRAPSEAAAAMEMGCGAVLVNSAVAVAGNPPEMANAFAEAVRAGRRAFKAELMP
KSSFALSTSPLTTFLGKKS

Sequences:

>Translated_259_residues
MDFLHLGSFVFSSRLILGTGKFSNADLMIEAIKASGAQLVTVALRRFNREQIADDLFGPLSALQGITLMPNTSGASTARE
AIHAAHIARELSGSPFIKVEIHPNPQHLMPDALETWEASRILAKEGFLVMPYIPADPVLAKRLEEVGCASVMPLGSAIGS
GQGLANAGMIELIIRESGIPVIVDAGLRAPSEAAAAMEMGCGAVLVNSAVAVAGNPPEMANAFAEAVRAGRRAFKAELMP
KSSFALSTSPLTTFLGKKS
>Mature_259_residues
MDFLHLGSFVFSSRLILGTGKFSNADLMIEAIKASGAQLVTVALRRFNREQIADDLFGPLSALQGITLMPNTSGASTARE
AIHAAHIARELSGSPFIKVEIHPNPQHLMPDALETWEASRILAKEGFLVMPYIPADPVLAKRLEEVGCASVMPLGSAIGS
GQGLANAGMIELIIRESGIPVIVDAGLRAPSEAAAAMEMGCGAVLVNSAVAVAGNPPEMANAFAEAVRAGRRAFKAELMP
KSSFALSTSPLTTFLGKKS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=253, Percent_Identity=54.5454545454545, Blast_Score=268, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_CHLPD (A1BEF7)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_911208.1
- ProteinModelPortal:   A1BEF7
- SMR:   A1BEF7
- STRING:   A1BEF7
- GeneID:   4569924
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0730
- NMPDR:   fig|290317.7.peg.769
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27151; Mature: 27151

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: ACT_SITE 98-98 BINDING 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFLHLGSFVFSSRLILGTGKFSNADLMIEAIKASGAQLVTVALRRFNREQIADDLFGPL
CCHHHHHHHHHHCEEEEECCCCCCCHHEEEEHHCCCCHHHHHHHHHCCHHHHHHHHHHHH
SALQGITLMPNTSGASTAREAIHAAHIARELSGSPFIKVEIHPNPQHLMPDALETWEASR
HHHCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHCCCHHHHHHHHHH
ILAKEGFLVMPYIPADPVLAKRLEEVGCASVMPLGSAIGSGQGLANAGMIELIIRESGIP
HHHHCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCC
VIVDAGLRAPSEAAAAMEMGCGAVLVNSAVAVAGNPPEMANAFAEAVRAGRRAFKAELMP
EEEECCCCCCHHHHHHHHHCCCCEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
KSSFALSTSPLTTFLGKKS
CCCCEECCCHHHHHHCCCC
>Mature Secondary Structure
MDFLHLGSFVFSSRLILGTGKFSNADLMIEAIKASGAQLVTVALRRFNREQIADDLFGPL
CCHHHHHHHHHHCEEEEECCCCCCCHHEEEEHHCCCCHHHHHHHHHCCHHHHHHHHHHHH
SALQGITLMPNTSGASTAREAIHAAHIARELSGSPFIKVEIHPNPQHLMPDALETWEASR
HHHCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHCCCHHHHHHHHHH
ILAKEGFLVMPYIPADPVLAKRLEEVGCASVMPLGSAIGSGQGLANAGMIELIIRESGIP
HHHHCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCC
VIVDAGLRAPSEAAAAMEMGCGAVLVNSAVAVAGNPPEMANAFAEAVRAGRRAFKAELMP
EEEECCCCCCHHHHHHHHHCCCCEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
KSSFALSTSPLTTFLGKKS
CCCCEECCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA