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The map label for this gene is nth [H]

Identifier: 119356338

GI number: 119356338

Start: 550085

End: 550735

Strand: Reverse

Name: nth [H]

Synonym: Cpha266_0501

Alternate gene names: 119356338

Gene position: 550735-550085 (Counterclockwise)

Preceding gene: 119356339

Following gene: 119356334

Centisome position: 17.57

GC content: 49.31

Gene sequence:

>651_bases
ATGCAACGCTCACCGAAGGAAAAAATAACCTTCATAAAAAAAGCTCTCGGCTCAGTTTATCCTGAACCGAAAAGCGAGCT
GCAGTACGCCTCAGCCTTTCAGCTCCTTATTGCAACCATCCTTGCAGCGCAGGCAACCGACAAAAAGGTAAATGAAGCAA
CCAAAGAGCTGTTTCTGCTCTGTCCTGATGCGCTGAGCATGAGTCGTACTGAACCTGATACGATCAAACAACTTGTTCGC
ACACTTAACTACTTCAACAACAAGGCAGCAAACATCCTGGCCGTAAGTTGCCGACTTGTTGAGGAGTTTAATGGAGAGGT
TCCCCCCAACAGGGAGGCGCTTGAATCGCTGCCGGGAGTTGGGAGAAAAACAGCCAACGTAGTGCTTGCCAATGCATTCG
GCCAACCGGTCATGCCGGTCGATACCCATGTGCACAGGGTATCCAACCGTATCGGGCTCTGCGCCACATCAAAACCCGAG
CAGACAGAAGAGGCCCTTACGAACATCATTCCCGAACCATGGATGATCGACTTTCATCACTACCTTTTACTTCACGGACG
CTATACCTGTAAAGCAAAAAAGCCGGCATGCGCAGATTGCGTGCTTAAGGAAATCTGCGCCTTTAAGGATAAAAATCCGA
TCGCAGACTAA

Upstream 100 bases:

>100_bases
ACGAACTGAAAGCCGAAGGAAAAATACACAAGAACGGAAGGGGTCTTATCGACCATTCGGCCATCAAACTGCAGAAAGAA
TCTGAAACTCCTGAATAGCC

Downstream 100 bases:

>100_bases
AAAAGCTCTATCTTTCTGTCAACGGCATAGACCCACTCTCCTTCGGGGCGAAGACGATTACCTGCCCAGAGTTCAAGCGT
AATGCCTTTTGGCGATACTG

Product: DNA-(apurinic or apyrimidinic site) lyase / endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MQRSPKEKITFIKKALGSVYPEPKSELQYASAFQLLIATILAAQATDKKVNEATKELFLLCPDALSMSRTEPDTIKQLVR
TLNYFNNKAANILAVSCRLVEEFNGEVPPNREALESLPGVGRKTANVVLANAFGQPVMPVDTHVHRVSNRIGLCATSKPE
QTEEALTNIIPEPWMIDFHHYLLLHGRYTCKAKKPACADCVLKEICAFKDKNPIAD

Sequences:

>Translated_216_residues
MQRSPKEKITFIKKALGSVYPEPKSELQYASAFQLLIATILAAQATDKKVNEATKELFLLCPDALSMSRTEPDTIKQLVR
TLNYFNNKAANILAVSCRLVEEFNGEVPPNREALESLPGVGRKTANVVLANAFGQPVMPVDTHVHRVSNRIGLCATSKPE
QTEEALTNIIPEPWMIDFHHYLLLHGRYTCKAKKPACADCVLKEICAFKDKNPIAD
>Mature_216_residues
MQRSPKEKITFIKKALGSVYPEPKSELQYASAFQLLIATILAAQATDKKVNEATKELFLLCPDALSMSRTEPDTIKQLVR
TLNYFNNKAANILAVSCRLVEEFNGEVPPNREALESLPGVGRKTANVVLANAFGQPVMPVDTHVHRVSNRIGLCATSKPE
QTEEALTNIIPEPWMIDFHHYLLLHGRYTCKAKKPACADCVLKEICAFKDKNPIAD

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=29.9435028248588, Blast_Score=91, Evalue=8e-19,
Organism=Escherichia coli, GI1787920, Length=192, Percent_Identity=47.9166666666667, Blast_Score=194, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17554540, Length=179, Percent_Identity=33.5195530726257, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6324530, Length=195, Percent_Identity=25.1282051282051, Blast_Score=76, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=31.638418079096, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24007; Mature: 24007

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRSPKEKITFIKKALGSVYPEPKSELQYASAFQLLIATILAAQATDKKVNEATKELFLL
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CPDALSMSRTEPDTIKQLVRTLNYFNNKAANILAVSCRLVEEFNGEVPPNREALESLPGV
CCCHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCC
GRKTANVVLANAFGQPVMPVDTHVHRVSNRIGLCATSKPEQTEEALTNIIPEPWMIDFHH
CCHHHHEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHH
YLLLHGRYTCKAKKPACADCVLKEICAFKDKNPIAD
HHHHHCCEEECCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQRSPKEKITFIKKALGSVYPEPKSELQYASAFQLLIATILAAQATDKKVNEATKELFLL
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CPDALSMSRTEPDTIKQLVRTLNYFNNKAANILAVSCRLVEEFNGEVPPNREALESLPGV
CCCHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCC
GRKTANVVLANAFGQPVMPVDTHVHRVSNRIGLCATSKPEQTEEALTNIIPEPWMIDFHH
CCHHHHEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHH
YLLLHGRYTCKAKKPACADCVLKEICAFKDKNPIAD
HHHHHCCEEECCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]