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The map label for this gene is rluB [H]

Identifier: 119356331

GI number: 119356331

Start: 540958

End: 541689

Strand: Direct

Name: rluB [H]

Synonym: Cpha266_0494

Alternate gene names: 119356331

Gene position: 540958-541689 (Clockwise)

Preceding gene: 119356330

Following gene: 119356332

Centisome position: 17.26

GC content: 49.73

Gene sequence:

>732_bases
ATGAAAGAAACGGAAGATGAGGGCGGGATACGAATCAACAGGTATCTTGCCATGTGCGGTATAGCTTCAAGACGGGCGGC
CGACAAGCTTGTTGCTGATGGTCGTGTGACGGTTAACGGCAAAATTGCAGATGAACCGGGATTAAGGATTCTGCCGTTCA
AGGACGAGGTTATTGTCGACGGGCGAAAATTTGTCGTGCCGGAAGCAAAAAAGGTCTATATCCTGCTCAATAAACCGAGA
AATGTTATTACCACCAGTCATGACGAGAAAGATCGTGAAACGATTCTTGATCTTGTATCCGTCGAGGAGCGGGTCTATCC
GGTAGGTCGGCTTGACAGGAAAACCACCGGCGTTATTCTTTTGACCAATGACGGAATTCTTGCTCATCGCCTGATGCACC
CGTCATCCAATGTTAAAAAAGAGTATGTTGCCGAGCTGGACAAACGCTTCCCCCAGGGGCTTCTTTCGCAGCTTGTCGGA
GGGATGAAGCTGAAGGATACCGGTGAAAAGGTGAGTCCCTGTCAGGCAAAACTCCTTGACGATGGCATGAGGATCTGGAT
CAGCATTCATGAAGGAAAAAATCACCAGGTGAGAAGAATGCTTGAAACGCTTGGTTTCGATGTAACAAAACTTGAACGGG
TCGCCTACGCTGGTCTGAAAGCCGGAGACCTCCGGCGTGGAGCCTGGCGCCACCTGAGCAAGAACGAAGTTCGGGAGTTG
TATCGATTGTAG

Upstream 100 bases:

>100_bases
AGAGGAGATTATACAGGAAAATGAGGAGCAGGAGTATCTTGCTTCGAGGGGTGGCGATAAAGATTATAACGGCAGGAACG
ATTGAAGGAGATCGGAGAAG

Downstream 100 bases:

>100_bases
TGCTTTTCAGTAGCAGCAGTTTTCAGCAACAGACCTGTTTTATAGCGTATGGCCCGTTTGCATCTCCTGCAGAGTTGCCG
CCGGATGTTTTTGTCCGTTC

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKETEDEGGIRINRYLAMCGIASRRAADKLVADGRVTVNGKIADEPGLRILPFKDEVIVDGRKFVVPEAKKVYILLNKPR
NVITTSHDEKDRETILDLVSVEERVYPVGRLDRKTTGVILLTNDGILAHRLMHPSSNVKKEYVAELDKRFPQGLLSQLVG
GMKLKDTGEKVSPCQAKLLDDGMRIWISIHEGKNHQVRRMLETLGFDVTKLERVAYAGLKAGDLRRGAWRHLSKNEVREL
YRL

Sequences:

>Translated_243_residues
MKETEDEGGIRINRYLAMCGIASRRAADKLVADGRVTVNGKIADEPGLRILPFKDEVIVDGRKFVVPEAKKVYILLNKPR
NVITTSHDEKDRETILDLVSVEERVYPVGRLDRKTTGVILLTNDGILAHRLMHPSSNVKKEYVAELDKRFPQGLLSQLVG
GMKLKDTGEKVSPCQAKLLDDGMRIWISIHEGKNHQVRRMLETLGFDVTKLERVAYAGLKAGDLRRGAWRHLSKNEVREL
YRL
>Mature_243_residues
MKETEDEGGIRINRYLAMCGIASRRAADKLVADGRVTVNGKIADEPGLRILPFKDEVIVDGRKFVVPEAKKVYILLNKPR
NVITTSHDEKDRETILDLVSVEERVYPVGRLDRKTTGVILLTNDGILAHRLMHPSSNVKKEYVAELDKRFPQGLLSQLVG
GMKLKDTGEKVSPCQAKLLDDGMRIWISIHEGKNHQVRRMLETLGFDVTKLERVAYAGLKAGDLRRGAWRHLSKNEVREL
YRL

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=240, Percent_Identity=32.9166666666667, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787524, Length=255, Percent_Identity=33.7254901960784, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1788510, Length=231, Percent_Identity=29.004329004329, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=32.5842696629214, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27507; Mature: 27507

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETEDEGGIRINRYLAMCGIASRRAADKLVADGRVTVNGKIADEPGLRILPFKDEVIVD
CCCCCCCCCEEEEHHHHHHCCHHHHHHHHHHCCCEEEECCEECCCCCCEEECCCCCEEEC
GRKFVVPEAKKVYILLNKPRNVITTSHDEKDRETILDLVSVEERVYPVGRLDRKTTGVIL
CCEEECCCCCEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
LTNDGILAHRLMHPSSNVKKEYVAELDKRFPQGLLSQLVGGMKLKDTGEKVSPCQAKLLD
EECCCCEEHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHC
DGMRIWISIHEGKNHQVRRMLETLGFDVTKLERVAYAGLKAGDLRRGAWRHLSKNEVREL
CCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHH
YRL
HCC
>Mature Secondary Structure
MKETEDEGGIRINRYLAMCGIASRRAADKLVADGRVTVNGKIADEPGLRILPFKDEVIVD
CCCCCCCCCEEEEHHHHHHCCHHHHHHHHHHCCCEEEECCEECCCCCCEEECCCCCEEEC
GRKFVVPEAKKVYILLNKPRNVITTSHDEKDRETILDLVSVEERVYPVGRLDRKTTGVIL
CCEEECCCCCEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
LTNDGILAHRLMHPSSNVKKEYVAELDKRFPQGLLSQLVGGMKLKDTGEKVSPCQAKLLD
EECCCCEEHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHC
DGMRIWISIHEGKNHQVRRMLETLGFDVTKLERVAYAGLKAGDLRRGAWRHLSKNEVREL
CCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHH
YRL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]