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The map label for this gene is gatA

Identifier: 119356238

GI number: 119356238

Start: 443441

End: 444871

Strand: Direct

Name: gatA

Synonym: Cpha266_0399

Alternate gene names: 119356238

Gene position: 443441-444871 (Clockwise)

Preceding gene: 119356234

Following gene: 119356239

Centisome position: 14.15

GC content: 49.34

Gene sequence:

>1431_bases
GTGCATTTCAGTAGTTATCAGGATTTGCGATCCAGCCTTCTTGCGGGACAGACGACCTGCGAAGAGGTAACAAAGAGTTA
TCTTGAGCGGATAGACCGGCATTGCGACGATAATATATTTCTTGCGGTTTTTCATGAACAGGCGTTGTCTCGGGCAAGAT
CGCTTGACAGGAGGTTTAGCGAGGGCGGCACGCCGGGGTTGTTGTTCGGAATGCCAATGGCTATAAAAGACAATATCTCC
ATTAAAGGGGCTCGTCTTACCTGCGCGTCGAGAATTCTTGAAAATTATGAGAGTGTCTATGATGCGACAGTTATCGAGCG
ACTTTTAAATGAGGATGCCGTTTTTATTGGAAAAACCAATATGGACGAGTTTGCAATGGGTAGTTCCAATGAAAATTCGG
CTTTCGGACCTGTAGCCAATCCGTTTGATAAAACGAGAGTTCCTGGCGGCAGTTCAGGTGGTTCAGCCGCAGCGGTTGCG
GCCGGCCTGGCTCTTGTTGCGCTCGGATCAGATACCGGAGGTTCGGTGCGTCAGCCTGCAGGGTTCTGCGATATTGTCGG
GTTGAAGCCAACTTACGGAAGGATTTCCCGTTACGGTCTTGTTGCTTTTGCCTCTTCGTTTGATCAGATTGGCGTTCTTG
CCCGAAATAGCGACGATGCCGCGCTCGTGCTCGGCGTTATGGCGGGTGCCGACGAGCGTGACGCAACCTCTTCACGTCAT
GCGGTATCAAATTATGCTGAAGAGATGGCCGCGGTTTCACCCGACGGTTTGAAAATTGGTGTTCCAAAGGAGTTTTTTCA
CGAGAGTCTGAATCCCGATGTGGCCCGTCTTGTAAAGGCAAAACTGGAGGAGCTGCGTGAAAAAGGGGCGGAACTGGTTG
ACATAACTCTCCCTGCAAGCGATTATGCCATTGCGGCATACTATATTCTTGTAACGGCGGAGGCTTCATCAAATCTTGCC
CGATTTGATGGTGCAAGATACGGGTATCGTTCACCGAACTGCGATAATCTCAGTGCGATGTATGTCAATTCAAGAACAGA
GGGGTTCGGCAAGGAGGTCAAGCGTCGGATCATGCTCGGTACCTATGTGCTTTCAGCCGGGTATTACGATACCTATTACA
AGAAGGCGCAGCAGGTTCGTCGTGTTTTTCAGGACAGATATCGTGAGGCGCTTGAAAAAGTCGATGTGATTGCCGGGCCG
ACATCACCGTTTCCGCCGTTCGGTATTGGCGATAAAATGGACGATCCTCTTGAAATGTATCTTGCTGATGTTTTTACCGT
TCCGGCAAGTATTGTCGGGATGCCTGCCATCAGTGTTCCGATTGGATATGACAGTCTTAATCTGCCAGTCGGCATGCATC
TGATCTGCAATTTCTTTGAGGAAGGAAAATTGCTTGGTATTGCCGGACTTATGCAGCATTCTATCGTTTAA

Upstream 100 bases:

>100_bases
TTACATGATAATGAAAAACTTGCTTGCCACCGACAACGTGCCTGAATGGGGAAAGTTGTTCCGGTTTCAGGCCTTCTTCA
TAAAATAATTCAGTACCACC

Downstream 100 bases:

>100_bases
GAAAAGAGGTGATTTGGTAAACCATTAATAAACAAGCAGGATTATGAGTGTTTTAGTCAATAAGGATACCCGCCTTGTTG
TACAGGGTATAACAGGTGGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MHFSSYQDLRSSLLAGQTTCEEVTKSYLERIDRHCDDNIFLAVFHEQALSRARSLDRRFSEGGTPGLLFGMPMAIKDNIS
IKGARLTCASRILENYESVYDATVIERLLNEDAVFIGKTNMDEFAMGSSNENSAFGPVANPFDKTRVPGGSSGGSAAAVA
AGLALVALGSDTGGSVRQPAGFCDIVGLKPTYGRISRYGLVAFASSFDQIGVLARNSDDAALVLGVMAGADERDATSSRH
AVSNYAEEMAAVSPDGLKIGVPKEFFHESLNPDVARLVKAKLEELREKGAELVDITLPASDYAIAAYYILVTAEASSNLA
RFDGARYGYRSPNCDNLSAMYVNSRTEGFGKEVKRRIMLGTYVLSAGYYDTYYKKAQQVRRVFQDRYREALEKVDVIAGP
TSPFPPFGIGDKMDDPLEMYLADVFTVPASIVGMPAISVPIGYDSLNLPVGMHLICNFFEEGKLLGIAGLMQHSIV

Sequences:

>Translated_476_residues
MHFSSYQDLRSSLLAGQTTCEEVTKSYLERIDRHCDDNIFLAVFHEQALSRARSLDRRFSEGGTPGLLFGMPMAIKDNIS
IKGARLTCASRILENYESVYDATVIERLLNEDAVFIGKTNMDEFAMGSSNENSAFGPVANPFDKTRVPGGSSGGSAAAVA
AGLALVALGSDTGGSVRQPAGFCDIVGLKPTYGRISRYGLVAFASSFDQIGVLARNSDDAALVLGVMAGADERDATSSRH
AVSNYAEEMAAVSPDGLKIGVPKEFFHESLNPDVARLVKAKLEELREKGAELVDITLPASDYAIAAYYILVTAEASSNLA
RFDGARYGYRSPNCDNLSAMYVNSRTEGFGKEVKRRIMLGTYVLSAGYYDTYYKKAQQVRRVFQDRYREALEKVDVIAGP
TSPFPPFGIGDKMDDPLEMYLADVFTVPASIVGMPAISVPIGYDSLNLPVGMHLICNFFEEGKLLGIAGLMQHSIV
>Mature_476_residues
MHFSSYQDLRSSLLAGQTTCEEVTKSYLERIDRHCDDNIFLAVFHEQALSRARSLDRRFSEGGTPGLLFGMPMAIKDNIS
IKGARLTCASRILENYESVYDATVIERLLNEDAVFIGKTNMDEFAMGSSNENSAFGPVANPFDKTRVPGGSSGGSAAAVA
AGLALVALGSDTGGSVRQPAGFCDIVGLKPTYGRISRYGLVAFASSFDQIGVLARNSDDAALVLGVMAGADERDATSSRH
AVSNYAEEMAAVSPDGLKIGVPKEFFHESLNPDVARLVKAKLEELREKGAELVDITLPASDYAIAAYYILVTAEASSNLA
RFDGARYGYRSPNCDNLSAMYVNSRTEGFGKEVKRRIMLGTYVLSAGYYDTYYKKAQQVRRVFQDRYREALEKVDVIAGP
TSPFPPFGIGDKMDDPLEMYLADVFTVPASIVGMPAISVPIGYDSLNLPVGMHLICNFFEEGKLLGIAGLMQHSIV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=499, Percent_Identity=36.0721442885772, Blast_Score=315, Evalue=7e-86,
Organism=Homo sapiens, GI195972892, Length=485, Percent_Identity=22.8865979381443, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI166795287, Length=145, Percent_Identity=35.8620689655172, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=459, Percent_Identity=35.0762527233115, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17537465, Length=484, Percent_Identity=24.7933884297521, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=303, Percent_Identity=29.3729372937294, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=314, Percent_Identity=27.0700636942675, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17556276, Length=305, Percent_Identity=29.5081967213115, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=305, Percent_Identity=29.5081967213115, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=196, Percent_Identity=29.5918367346939, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17538254, Length=196, Percent_Identity=27.0408163265306, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=426, Percent_Identity=37.0892018779343, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=445, Percent_Identity=30.7865168539326, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320448, Length=195, Percent_Identity=28.2051282051282, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648113, Length=486, Percent_Identity=36.6255144032922, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24652985, Length=479, Percent_Identity=26.5135699373695, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19922090, Length=479, Percent_Identity=26.5135699373695, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24652981, Length=479, Percent_Identity=26.5135699373695, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24652983, Length=479, Percent_Identity=26.5135699373695, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI45550774, Length=487, Percent_Identity=26.0780287474333, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24648435, Length=487, Percent_Identity=26.0780287474333, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24648437, Length=487, Percent_Identity=26.0780287474333, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24648441, Length=416, Percent_Identity=25.9615384615385, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648439, Length=416, Percent_Identity=25.9615384615385, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24644968, Length=493, Percent_Identity=22.7180527383367, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI161078093, Length=478, Percent_Identity=23.8493723849372, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21356731, Length=485, Percent_Identity=24.3298969072165, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_CHLPD (A1BDI1)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_910882.1
- ProteinModelPortal:   A1BDI1
- SMR:   A1BDI1
- STRING:   A1BDI1
- GeneID:   4568654
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0399
- NMPDR:   fig|290317.7.peg.410
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51584; Mature: 51584

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 76-76 ACT_SITE 151-151 ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFSSYQDLRSSLLAGQTTCEEVTKSYLERIDRHCDDNIFLAVFHEQALSRARSLDRRFS
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHH
EGGTPGLLFGMPMAIKDNISIKGARLTCASRILENYESVYDATVIERLLNEDAVFIGKTN
CCCCCCCCCCCCEEEECCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
MDEFAMGSSNENSAFGPVANPFDKTRVPGGSSGGSAAAVAAGLALVALGSDTGGSVRQPA
CHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHEEEECCCCCCCCCCCC
GFCDIVGLKPTYGRISRYGLVAFASSFDQIGVLARNSDDAALVLGVMAGADERDATSSRH
CCEEEECCCCCHHHHHHHHHHHHHHCHHHHCEEEECCCCCEEEEEEHHCCCCCCCCHHHH
AVSNYAEEMAAVSPDGLKIGVPKEFFHESLNPDVARLVKAKLEELREKGAELVDITLPAS
HHHHHHHHHHHCCCCCCEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DYAIAAYYILVTAEASSNLARFDGARYGYRSPNCDNLSAMYVNSRTEGFGKEVKRRIMLG
CHHEEEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHH
TYVLSAGYYDTYYKKAQQVRRVFQDRYREALEKVDVIAGPTSPFPPFGIGDKMDDPLEMY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHH
LADVFTVPASIVGMPAISVPIGYDSLNLPVGMHLICNFFEEGKLLGIAGLMQHSIV
HHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEHHHHHHHHCCC
>Mature Secondary Structure
MHFSSYQDLRSSLLAGQTTCEEVTKSYLERIDRHCDDNIFLAVFHEQALSRARSLDRRFS
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHH
EGGTPGLLFGMPMAIKDNISIKGARLTCASRILENYESVYDATVIERLLNEDAVFIGKTN
CCCCCCCCCCCCEEEECCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
MDEFAMGSSNENSAFGPVANPFDKTRVPGGSSGGSAAAVAAGLALVALGSDTGGSVRQPA
CHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHEEEECCCCCCCCCCCC
GFCDIVGLKPTYGRISRYGLVAFASSFDQIGVLARNSDDAALVLGVMAGADERDATSSRH
CCEEEECCCCCHHHHHHHHHHHHHHCHHHHCEEEECCCCCEEEEEEHHCCCCCCCCHHHH
AVSNYAEEMAAVSPDGLKIGVPKEFFHESLNPDVARLVKAKLEELREKGAELVDITLPAS
HHHHHHHHHHHCCCCCCEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DYAIAAYYILVTAEASSNLARFDGARYGYRSPNCDNLSAMYVNSRTEGFGKEVKRRIMLG
CHHEEEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHH
TYVLSAGYYDTYYKKAQQVRRVFQDRYREALEKVDVIAGPTSPFPPFGIGDKMDDPLEMY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHH
LADVFTVPASIVGMPAISVPIGYDSLNLPVGMHLICNFFEEGKLLGIAGLMQHSIV
HHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA