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The map label for this gene is folC [H]

Identifier: 119356230

GI number: 119356230

Start: 432457

End: 433743

Strand: Direct

Name: folC [H]

Synonym: Cpha266_0391

Alternate gene names: 119356230

Gene position: 432457-433743 (Clockwise)

Preceding gene: 119356229

Following gene: 119356231

Centisome position: 13.8

GC content: 49.49

Gene sequence:

>1287_bases
GTGAATTACCAGGAAGCACTTGATTTTCTCTATCCTCTGCACCGTTTCGGTATCAAGCCCGGTCTTGAAAGGGTTTTCGG
TCTGCTTGCTGTTCTCGGTTCACCACATCGGCGATTGGGTACGATTGTTCATGTTTCCGGTACTAACGGGAAAGGAACAA
CAGCTGCTGCAATTGCTGCGATTTTTCAGGCAGCCGGCAAAAAGACTGCACTCTACACCTCGCCGCATCTGGTTGATTTT
ACTGAAAGGATGCGTATCAACGGTGGGTGTATACCTCATGAGCTTGTTGCAACGTACTGCTCTCTTATCAAGTCCAGGGT
TGAAGAGAGTCATACAACTTTTTTTGAAGCAACAACAGCCATTGCTTTTGCCTGGTTTGCTTCCGAACACGTTGAGGTAA
CAATTGTTGAGACCGGTATGGGCGGGAGACTTGATGCTACTAACGTTGTTCATGCTGATTACGTAGCGATTACTTCAATA
GGGCAGGATCATACCGCATGGCTTGGTTCGACCCCTGCGTTGATTGCTGCGGAAAAAGCGGCGATCATTAAAAAAGGATC
GCAAGTATTTTCTGCCGTAACAGATTCTGAGGCGGCAATGCCTGTAAGAACGGCAGCAGAGTGTTGTGAAGCCCCCCTCT
CTGTTCTTGGTATTGATGCACACTGTCATGTTGACAGCGAAGAGATTGGTCGGCTTGAACTTTCGGTGAGAACGGCAAAG
CATCAGTATGAAAGGCTTACGGTTCCTCTGACCGGAGCATTTCATGCCTCAAACATCTCTCTTGCGGTGATGGTTGCAGA
AACGGCCGGCATTGATGAGTCATCTATCCGCAATGGGCTTGCAAACCTTTTGCAAACGGGTTACCGGGCCAGGCTTGAAA
AGATAGCAGCGAATCCTGACGTCTTGCTGGATGTGTCGCACAATGCGAACGGCATTGAAGAGACCGTCAACACGCTTGCC
CGTTTCAGAAATCGTTATCGGAATCTGTTTGTGCTCATCGGGCTTGCCTCCGATAAAAATGCCACAGCAATCGTCAAACA
TCTCAAGCGGCTATCATGCCGACTGGTTGCGGTTTCCATCCCTTCCGAAAGAAGTATTCCGGCTTTGGAGCTTGGCGGGA
TTGGAGAGGCTGAAGATATTCCGACTATGGTGTTCAGTTCGCCTCTTGATGGGCTTGGCTATCTTTCAGGCATCGCCGAA
GCTGAGGATCTGGTACTGGTTACCGGATCGTTCTATCTGGCAGGCGAGGTGCTTTCAAACGGTGGCTGGAACAAGGAGAT
GAGCTGA

Upstream 100 bases:

>100_bases
AGCCGTTTCGGCTCTTCTTGAAGAGAAGCCGGAACAATCCGTTGAAGAGGTCATTAAGTATGCACTCGCGACCATTCATA
ACAGTTAACCCCGATTTTCG

Downstream 100 bases:

>100_bases
TGAGCGCGTTGTTAAGTTTTTTTCCCGAAAAAAATTATATATTTATTATAACTACAGAGGTTCTCCATGATTTATGGGTA
ATACTTCCAGGCCGTTTTTT

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNYQEALDFLYPLHRFGIKPGLERVFGLLAVLGSPHRRLGTIVHVSGTNGKGTTAAAIAAIFQAAGKKTALYTSPHLVDF
TERMRINGGCIPHELVATYCSLIKSRVEESHTTFFEATTAIAFAWFASEHVEVTIVETGMGGRLDATNVVHADYVAITSI
GQDHTAWLGSTPALIAAEKAAIIKKGSQVFSAVTDSEAAMPVRTAAECCEAPLSVLGIDAHCHVDSEEIGRLELSVRTAK
HQYERLTVPLTGAFHASNISLAVMVAETAGIDESSIRNGLANLLQTGYRARLEKIAANPDVLLDVSHNANGIEETVNTLA
RFRNRYRNLFVLIGLASDKNATAIVKHLKRLSCRLVAVSIPSERSIPALELGGIGEAEDIPTMVFSSPLDGLGYLSGIAE
AEDLVLVTGSFYLAGEVLSNGGWNKEMS

Sequences:

>Translated_428_residues
MNYQEALDFLYPLHRFGIKPGLERVFGLLAVLGSPHRRLGTIVHVSGTNGKGTTAAAIAAIFQAAGKKTALYTSPHLVDF
TERMRINGGCIPHELVATYCSLIKSRVEESHTTFFEATTAIAFAWFASEHVEVTIVETGMGGRLDATNVVHADYVAITSI
GQDHTAWLGSTPALIAAEKAAIIKKGSQVFSAVTDSEAAMPVRTAAECCEAPLSVLGIDAHCHVDSEEIGRLELSVRTAK
HQYERLTVPLTGAFHASNISLAVMVAETAGIDESSIRNGLANLLQTGYRARLEKIAANPDVLLDVSHNANGIEETVNTLA
RFRNRYRNLFVLIGLASDKNATAIVKHLKRLSCRLVAVSIPSERSIPALELGGIGEAEDIPTMVFSSPLDGLGYLSGIAE
AEDLVLVTGSFYLAGEVLSNGGWNKEMS
>Mature_428_residues
MNYQEALDFLYPLHRFGIKPGLERVFGLLAVLGSPHRRLGTIVHVSGTNGKGTTAAAIAAIFQAAGKKTALYTSPHLVDF
TERMRINGGCIPHELVATYCSLIKSRVEESHTTFFEATTAIAFAWFASEHVEVTIVETGMGGRLDATNVVHADYVAITSI
GQDHTAWLGSTPALIAAEKAAIIKKGSQVFSAVTDSEAAMPVRTAAECCEAPLSVLGIDAHCHVDSEEIGRLELSVRTAK
HQYERLTVPLTGAFHASNISLAVMVAETAGIDESSIRNGLANLLQTGYRARLEKIAANPDVLLDVSHNANGIEETVNTLA
RFRNRYRNLFVLIGLASDKNATAIVKHLKRLSCRLVAVSIPSERSIPALELGGIGEAEDIPTMVFSSPLDGLGYLSGIAE
AEDLVLVTGSFYLAGEVLSNGGWNKEMS

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=181, Percent_Identity=37.5690607734807, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI66932984, Length=180, Percent_Identity=37.7777777777778, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1788654, Length=417, Percent_Identity=31.89448441247, Blast_Score=161, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI71984923, Length=443, Percent_Identity=26.1851015801354, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17553148, Length=443, Percent_Identity=26.1851015801354, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17553150, Length=443, Percent_Identity=26.1851015801354, Blast_Score=111, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323760, Length=435, Percent_Identity=28.0459770114943, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324815, Length=258, Percent_Identity=31.3953488372093, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24641571, Length=170, Percent_Identity=36.4705882352941, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24581568, Length=196, Percent_Identity=31.1224489795918, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 45840; Mature: 45840

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYQEALDFLYPLHRFGIKPGLERVFGLLAVLGSPHRRLGTIVHVSGTNGKGTTAAAIAA
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHCCEEEEEECCCCCCHHHHHHHH
IFQAAGKKTALYTSPHLVDFTERMRINGGCIPHELVATYCSLIKSRVEESHTTFFEATTA
HHHHCCCCEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAFAWFASEHVEVTIVETGMGGRLDATNVVHADYVAITSIGQDHTAWLGSTPALIAAEKA
HHHHEECCCCEEEEEEEECCCCCCCCCCEEEECEEEEEECCCCCCHHCCCCCHHHHHHHH
AIIKKGSQVFSAVTDSEAAMPVRTAAECCEAPLSVLGIDAHCHVDSEEIGRLELSVRTAK
HHHHCCHHHHHHHCCCCCCCCHHHHHHHHHCCHHHEEECEEECCCHHHHCEEEEEEEHHH
HQYERLTVPLTGAFHASNISLAVMVAETAGIDESSIRNGLANLLQTGYRARLEKIAANPD
HHHHEEEEEEECCEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLLDVSHNANGIEETVNTLARFRNRYRNLFVLIGLASDKNATAIVKHLKRLSCRLVAVSI
EEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHEEEEEEEC
PSERSIPALELGGIGEAEDIPTMVFSSPLDGLGYLSGIAEAEDLVLVTGSFYLAGEVLSN
CCCCCCCEEEECCCCCCCCCCHHHHCCCCCHHHHHHHCCCCCCEEEEECCCEEEHHHHHC
GGWNKEMS
CCCCCCCC
>Mature Secondary Structure
MNYQEALDFLYPLHRFGIKPGLERVFGLLAVLGSPHRRLGTIVHVSGTNGKGTTAAAIAA
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHCCEEEEEECCCCCCHHHHHHHH
IFQAAGKKTALYTSPHLVDFTERMRINGGCIPHELVATYCSLIKSRVEESHTTFFEATTA
HHHHCCCCEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAFAWFASEHVEVTIVETGMGGRLDATNVVHADYVAITSIGQDHTAWLGSTPALIAAEKA
HHHHEECCCCEEEEEEEECCCCCCCCCCEEEECEEEEEECCCCCCHHCCCCCHHHHHHHH
AIIKKGSQVFSAVTDSEAAMPVRTAAECCEAPLSVLGIDAHCHVDSEEIGRLELSVRTAK
HHHHCCHHHHHHHCCCCCCCCHHHHHHHHHCCHHHEEECEEECCCHHHHCEEEEEEEHHH
HQYERLTVPLTGAFHASNISLAVMVAETAGIDESSIRNGLANLLQTGYRARLEKIAANPD
HHHHEEEEEEECCEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLLDVSHNANGIEETVNTLARFRNRYRNLFVLIGLASDKNATAIVKHLKRLSCRLVAVSI
EEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHEEEEEEEC
PSERSIPALELGGIGEAEDIPTMVFSSPLDGLGYLSGIAEAEDLVLVTGSFYLAGEVLSN
CCCCCCCEEEECCCCCCCCCCHHHHCCCCCHHHHHHHCCCCCCEEEEECCCEEEHHHHHC
GGWNKEMS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]