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The map label for this gene is htrB [H]

Identifier: 119356186

GI number: 119356186

Start: 383525

End: 384421

Strand: Direct

Name: htrB [H]

Synonym: Cpha266_0347

Alternate gene names: 119356186

Gene position: 383525-384421 (Clockwise)

Preceding gene: 119356179

Following gene: 119356192

Centisome position: 12.24

GC content: 47.49

Gene sequence:

>897_bases
ATGACCGATCCAAGTATAACCAACAAGCAATCCTCAGACAGGCGCATCTATAAGCTTTTTATGCTCTTCAGTGTTCTTGT
CAGAAAAATGAACCGAAAAACGGCACTTTTCATGGCAGATCGGCTTGGAGATTTGATGTTTGATATTCTTAAAATCCGCA
AACCCCTTGTTGAGGGGAACCTTTCGATAACCTTTCCGGAAAAAAGCGCCAACGAAATTCGTTCGATAGCAAGGCGGGTA
TACAGAAACCAGGCGAGAAATTTTATCGAGGTGCTTCGTCTCCCGCTTATCGCCAGCCGGGAAGATGCTGAAGAGCTTTT
CGATATCAATGCATCCCAACTGTCCGACATTTTGCAGCGATCGGGGGTTGTGCTTGTTTCAGCTCATTTCGGTAACTGGG
AACTGCTTGGGTTTTGCGCAGGGATCATGGTAACGCCTCTGACAATTGTTGTAAAGCGGTTAAGGAATGATGACGTCGAT
CGCTGTATCAACACCTGGAGAACCATGAAGGGTAACACTGTCGTAAAAAAGTCGCGAGCTCTGCGTGAAGGGTTGAAAAC
ACTCAGGAACAAAGGGATAGTGTCCTTTCTTGCAGATCAGTCAGATCCTGAGGGCACGTTTGTTACAGACTTTCTCGGCA
GACCATCATCAGTGTTTCTCGGCCCGGCCTATCTGGCTCTGAAAACCAGGGTGCCTCTTTTTGTCTGTATGGCATACAGG
AAAGAGGATGGACGTCATACCGTAGATATTGAACAGATTCCGACGGAAGACCTCAAAGATAGCCGGGAGGACTTTGAAGA
GCTCGCCCGTCGTTATACCAGAGCGATTGATAAAGCTATCAGAGTTCGACCTGAAGAGTGGTTCTGGCTACACGACCGCT
GGAAACGGACGTTTTGA

Upstream 100 bases:

>100_bases
ACAGTGACTGGTATTTGACTGAACCGGTAAATTCAGGTATTTTCAGGCATCTGTGCGGAAGGGATGCTGTTTATTGCACG
GGATTAAATCAAAGAAATTC

Downstream 100 bases:

>100_bases
TCTCTATGGCTGAAATTCTCTGTGTTTTGTTTTGAAAATAAGAATCAGTCATATTGCCTGAATTCGTCAATATTCTGTCT
CAAGAACTTTTATTGTTTTC

Product: lipid A biosynthesis acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTDPSITNKQSSDRRIYKLFMLFSVLVRKMNRKTALFMADRLGDLMFDILKIRKPLVEGNLSITFPEKSANEIRSIARRV
YRNQARNFIEVLRLPLIASREDAEELFDINASQLSDILQRSGVVLVSAHFGNWELLGFCAGIMVTPLTIVVKRLRNDDVD
RCINTWRTMKGNTVVKKSRALREGLKTLRNKGIVSFLADQSDPEGTFVTDFLGRPSSVFLGPAYLALKTRVPLFVCMAYR
KEDGRHTVDIEQIPTEDLKDSREDFEELARRYTRAIDKAIRVRPEEWFWLHDRWKRTF

Sequences:

>Translated_298_residues
MTDPSITNKQSSDRRIYKLFMLFSVLVRKMNRKTALFMADRLGDLMFDILKIRKPLVEGNLSITFPEKSANEIRSIARRV
YRNQARNFIEVLRLPLIASREDAEELFDINASQLSDILQRSGVVLVSAHFGNWELLGFCAGIMVTPLTIVVKRLRNDDVD
RCINTWRTMKGNTVVKKSRALREGLKTLRNKGIVSFLADQSDPEGTFVTDFLGRPSSVFLGPAYLALKTRVPLFVCMAYR
KEDGRHTVDIEQIPTEDLKDSREDFEELARRYTRAIDKAIRVRPEEWFWLHDRWKRTF
>Mature_297_residues
TDPSITNKQSSDRRIYKLFMLFSVLVRKMNRKTALFMADRLGDLMFDILKIRKPLVEGNLSITFPEKSANEIRSIARRVY
RNQARNFIEVLRLPLIASREDAEELFDINASQLSDILQRSGVVLVSAHFGNWELLGFCAGIMVTPLTIVVKRLRNDDVDR
CINTWRTMKGNTVVKKSRALREGLKTLRNKGIVSFLADQSDPEGTFVTDFLGRPSSVFLGPAYLALKTRVPLFVCMAYRK
EDGRHTVDIEQIPTEDLKDSREDFEELARRYTRAIDKAIRVRPEEWFWLHDRWKRTF

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=257, Percent_Identity=26.0700389105058, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 34607; Mature: 34475

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPSITNKQSSDRRIYKLFMLFSVLVRKMNRKTALFMADRLGDLMFDILKIRKPLVEGN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LSITFPEKSANEIRSIARRVYRNQARNFIEVLRLPLIASREDAEELFDINASQLSDILQR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHH
SGVVLVSAHFGNWELLGFCAGIMVTPLTIVVKRLRNDDVDRCINTWRTMKGNTVVKKSRA
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHH
LREGLKTLRNKGIVSFLADQSDPEGTFVTDFLGRPSSVFLGPAYLALKTRVPLFVCMAYR
HHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHCCCCCEEECHHHHHHHHCCHHHHHHHHH
KEDGRHTVDIEQIPTEDLKDSREDFEELARRYTRAIDKAIRVRPEEWFWLHDRWKRTF
HCCCCEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHCCCC
>Mature Secondary Structure 
TDPSITNKQSSDRRIYKLFMLFSVLVRKMNRKTALFMADRLGDLMFDILKIRKPLVEGN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LSITFPEKSANEIRSIARRVYRNQARNFIEVLRLPLIASREDAEELFDINASQLSDILQR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHH
SGVVLVSAHFGNWELLGFCAGIMVTPLTIVVKRLRNDDVDRCINTWRTMKGNTVVKKSRA
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHH
LREGLKTLRNKGIVSFLADQSDPEGTFVTDFLGRPSSVFLGPAYLALKTRVPLFVCMAYR
HHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHCCCCCEEECHHHHHHHHCCHHHHHHHHH
KEDGRHTVDIEQIPTEDLKDSREDFEELARRYTRAIDKAIRVRPEEWFWLHDRWKRTF
HCCCCEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]