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The map label for this gene is mrcA [H]

Identifier: 119356143

GI number: 119356143

Start: 333447

End: 335729

Strand: Reverse

Name: mrcA [H]

Synonym: Cpha266_0301

Alternate gene names: 119356143

Gene position: 335729-333447 (Counterclockwise)

Preceding gene: 119356144

Following gene: 119356142

Centisome position: 10.71

GC content: 50.33

Gene sequence:

>2283_bases
ATGATAACCTTAAGAACGACTGTTACCGTGAATACTACTTTTTTTAAAAAATCTTTTATTACTCTTGCCGCGCTGGTTTT
TTTTCTGCTGACTTCGGTCAGCGCATTTGCGCTCAATCCTTTCAAGGGGCTTCCAAGCCTTGAAGAACTTGAAAACCCTA
ACCCGGATCTCGCATCACTGGTCTATTCGGAAGACGGCGTGCTGCTTCACAAGTTTTTCGTAAAAAACCGCACGTTTATC
CCGCTGAAATCAATTCCGAGATCTGCGCGTTATGCTCTTATAGCCACGGAAGATGTAACGTTTTATCAGCACTGGGGTGT
TGATCTTCGCCGTCTCGCACTGGTCATGGGTGAAAATATCATCAAGGGACGCCAGAGATGGCACGGCGCGAGCACGATCA
CGCAGCAGCTTGCCAAAAACCTCTACCTGACGCAGGAAAGAACCGTCTCAAGAAAAGTCAAGGAGCTTATTACCGCTGTT
GAGCTTGAAAAAACCTATACAAAAGATGAGATCCTGGCACTGTATCTCAACACAGTCTATTTCGGTTCCGGAGCCTATGG
CATTGAAGCTGCAGCAAGAACCTATTTCAGCAAACCGGCCAGCCAACTGACCCTCCCGGAGAGCGCAACACTCATTGCAA
CCCTGAAAAACCCTACCGGGTACAACCCGGTCAAGAATCCATCCGGAGCCATCAGCAGACGGAACCTGATTCTGTCATTA
ATGGAAAAGGAAAAATTCATAACCCCGGAGCAGGCCGCTACGGCAAAAAGAAGCCGGCTTCTATTGCACTACACCCCTGT
ATCGCACCATGGAATCGCCCCTTATTTCACGGAATATATACGCCAAACAGTAAAACCAGCCTCAATGCTCGGCGATATCA
ATCTGCATCGCGACGGGCTGACCATTCAGACCACGCTTGACAGTCGCATGCAGAAATATGCTGAACAGGCCGCTGTCGAT
CACCTTGCATCATTACAGGCATCCTTCGATCGATCATGGAGATGGCCGGAATCGCTTAAAAACCAGATGATAAAGGAGAG
CCCCAGATTCAAGGAACTTCTGGAGGATGGAGTATCGGCACAGCAGGCCCTCGTGCAGCTCAGGGCTGACAGGATCTGGC
TGAATAATCTGTTGCGTGAAAAAACAAGAATACAGGTAGCCTTGGTCGCCATTGACCCTGCAAACGGTCATATCAAGGCA
TGGGTTGGCGGCAACAGCCTCTCTTCAGAGGACTACAAATATCAGTTTGACCATGTATGGCAGGCTAAAAGGCAGCCGGG
ATCGACCTTTAAACCATTTGTCTACCTGACGGCAATCGACAAGGGAATTCCTGCAAATTTCAGGGTTCTTGACCAGCCGC
TGGCTCTTAAAAGCGGCAATGGCATCTGGTCGCCAAGAAACTCGGACGGTTCATCAGGAGGGATGACAACACTCCGCTCT
GCGCTGACCCGTTCTCTCAATCAGGTATCCGTACGACTTGCATATGAGCAGCTTACGGTTCCGGAAATTATCAGCTATGC
AAAGAGAATGGGCATTTCCTCAGTTATGCCGCAAAACTATTCCATCGCGCTTGGAACCGGTGAAGTTAGCCCACTTGAGC
TTGCCGGAGCATTTTCGACTTTCGCCAACAATGGCATCTGGACCGAACCGGTTTCGATTCTGAAAGTTTCGGACAAACAT
CACCGATCCATGTCGGAATCCACACCCAACAGACGTTTCGCCATTGACTCGACCTCCAACTTCGTCATGGTATCAATGAT
GCGGGATGTTATCAACAGAGGAACAGCCTCTTCTATACGAGGCAGATATGCCATGACAATGGATGCTGCCGGCAAAACAG
GAACCACGCAGAGTCTCAGGGATGCCTGGTTCGCCGGGTTTACCCCGCAACTTGTTGCTGTGGTGTGGACCGGTTTTGAT
GATGAGCGCATCAAGTTTACCTCCATGGAGTATGGGCAAGGTGCACGGGCTGCTCTTCCGATATGGGCGGGCTTCATGAA
ACGGTGCTACAGCGACCCTTCTCTGAAACTGACAAACAGATACTTCCATATTCCAAACACGGTTATTGCAGTCCCCATTT
CCGGCCAGGCCAGTGTGGCCTCTGCTCCTGCGAACAACTCGGTGTATATCGAGTACTATACACCAAAGGGATTCAACTAT
TACAAGTCAAACCCTGTCCAGTCAACACCGGGAGACCAGGGAGACAGCGGCGTACCGACTGAAGGCGATCTCGAACCGGC
CCCACAACCGGCAGAGACTCCCGCACAGGAAGGGGCGTTTTGA

Upstream 100 bases:

>100_bases
CTCTGCCAGAAAATTTCATGAAAACAACTCAACCGAACCTGCTCGTTGCAAACAATACCAGATTTCATGATTTTCATTAC
CGACTCACCCATGCCGACCT

Downstream 100 bases:

>100_bases
TACTTTCTCAATTAACAAACTGTTTTTTACCATGCAATCGGTAACCGTTCTTGACTTTGGATCTCAATATACCCAGCTTA
TCGCCCGACGTATCCGCGAA

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 760; Mature: 760

Protein sequence:

>760_residues
MITLRTTVTVNTTFFKKSFITLAALVFFLLTSVSAFALNPFKGLPSLEELENPNPDLASLVYSEDGVLLHKFFVKNRTFI
PLKSIPRSARYALIATEDVTFYQHWGVDLRRLALVMGENIIKGRQRWHGASTITQQLAKNLYLTQERTVSRKVKELITAV
ELEKTYTKDEILALYLNTVYFGSGAYGIEAAARTYFSKPASQLTLPESATLIATLKNPTGYNPVKNPSGAISRRNLILSL
MEKEKFITPEQAATAKRSRLLLHYTPVSHHGIAPYFTEYIRQTVKPASMLGDINLHRDGLTIQTTLDSRMQKYAEQAAVD
HLASLQASFDRSWRWPESLKNQMIKESPRFKELLEDGVSAQQALVQLRADRIWLNNLLREKTRIQVALVAIDPANGHIKA
WVGGNSLSSEDYKYQFDHVWQAKRQPGSTFKPFVYLTAIDKGIPANFRVLDQPLALKSGNGIWSPRNSDGSSGGMTTLRS
ALTRSLNQVSVRLAYEQLTVPEIISYAKRMGISSVMPQNYSIALGTGEVSPLELAGAFSTFANNGIWTEPVSILKVSDKH
HRSMSESTPNRRFAIDSTSNFVMVSMMRDVINRGTASSIRGRYAMTMDAAGKTGTTQSLRDAWFAGFTPQLVAVVWTGFD
DERIKFTSMEYGQGARAALPIWAGFMKRCYSDPSLKLTNRYFHIPNTVIAVPISGQASVASAPANNSVYIEYYTPKGFNY
YKSNPVQSTPGDQGDSGVPTEGDLEPAPQPAETPAQEGAF

Sequences:

>Translated_760_residues
MITLRTTVTVNTTFFKKSFITLAALVFFLLTSVSAFALNPFKGLPSLEELENPNPDLASLVYSEDGVLLHKFFVKNRTFI
PLKSIPRSARYALIATEDVTFYQHWGVDLRRLALVMGENIIKGRQRWHGASTITQQLAKNLYLTQERTVSRKVKELITAV
ELEKTYTKDEILALYLNTVYFGSGAYGIEAAARTYFSKPASQLTLPESATLIATLKNPTGYNPVKNPSGAISRRNLILSL
MEKEKFITPEQAATAKRSRLLLHYTPVSHHGIAPYFTEYIRQTVKPASMLGDINLHRDGLTIQTTLDSRMQKYAEQAAVD
HLASLQASFDRSWRWPESLKNQMIKESPRFKELLEDGVSAQQALVQLRADRIWLNNLLREKTRIQVALVAIDPANGHIKA
WVGGNSLSSEDYKYQFDHVWQAKRQPGSTFKPFVYLTAIDKGIPANFRVLDQPLALKSGNGIWSPRNSDGSSGGMTTLRS
ALTRSLNQVSVRLAYEQLTVPEIISYAKRMGISSVMPQNYSIALGTGEVSPLELAGAFSTFANNGIWTEPVSILKVSDKH
HRSMSESTPNRRFAIDSTSNFVMVSMMRDVINRGTASSIRGRYAMTMDAAGKTGTTQSLRDAWFAGFTPQLVAVVWTGFD
DERIKFTSMEYGQGARAALPIWAGFMKRCYSDPSLKLTNRYFHIPNTVIAVPISGQASVASAPANNSVYIEYYTPKGFNY
YKSNPVQSTPGDQGDSGVPTEGDLEPAPQPAETPAQEGAF
>Mature_760_residues
MITLRTTVTVNTTFFKKSFITLAALVFFLLTSVSAFALNPFKGLPSLEELENPNPDLASLVYSEDGVLLHKFFVKNRTFI
PLKSIPRSARYALIATEDVTFYQHWGVDLRRLALVMGENIIKGRQRWHGASTITQQLAKNLYLTQERTVSRKVKELITAV
ELEKTYTKDEILALYLNTVYFGSGAYGIEAAARTYFSKPASQLTLPESATLIATLKNPTGYNPVKNPSGAISRRNLILSL
MEKEKFITPEQAATAKRSRLLLHYTPVSHHGIAPYFTEYIRQTVKPASMLGDINLHRDGLTIQTTLDSRMQKYAEQAAVD
HLASLQASFDRSWRWPESLKNQMIKESPRFKELLEDGVSAQQALVQLRADRIWLNNLLREKTRIQVALVAIDPANGHIKA
WVGGNSLSSEDYKYQFDHVWQAKRQPGSTFKPFVYLTAIDKGIPANFRVLDQPLALKSGNGIWSPRNSDGSSGGMTTLRS
ALTRSLNQVSVRLAYEQLTVPEIISYAKRMGISSVMPQNYSIALGTGEVSPLELAGAFSTFANNGIWTEPVSILKVSDKH
HRSMSESTPNRRFAIDSTSNFVMVSMMRDVINRGTASSIRGRYAMTMDAAGKTGTTQSLRDAWFAGFTPQLVAVVWTGFD
DERIKFTSMEYGQGARAALPIWAGFMKRCYSDPSLKLTNRYFHIPNTVIAVPISGQASVASAPANNSVYIEYYTPKGFNY
YKSNPVQSTPGDQGDSGVPTEGDLEPAPQPAETPAQEGAF

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=616, Percent_Identity=31.3311688311688, Blast_Score=254, Evalue=2e-68,
Organism=Escherichia coli, GI1786343, Length=613, Percent_Identity=30.3425774877651, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1788867, Length=635, Percent_Identity=26.2992125984252, Blast_Score=146, Evalue=5e-36,
Organism=Escherichia coli, GI1789601, Length=205, Percent_Identity=38.5365853658537, Blast_Score=119, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 84462; Mature: 84462

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLRTTVTVNTTFFKKSFITLAALVFFLLTSVSAFALNPFKGLPSLEELENPNPDLASL
CEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHE
VYSEDGVLLHKFFVKNRTFIPLKSIPRSARYALIATEDVTFYQHWGVDLRRLALVMGENI
EECCCCEEEEHHHHCCCCEEEHHHCCCCCCEEEEEECCEEEHHHCCCHHHHHHHHHCCHH
IKGRQRWHGASTITQQLAKNLYLTQERTVSRKVKELITAVELEKTYTKDEILALYLNTVY
HHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEHHHE
FGSGAYGIEAAARTYFSKPASQLTLPESATLIATLKNPTGYNPVKNPSGAISRRNLILSL
ECCCCCHHHHHHHHHHCCCCHHEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
MEKEKFITPEQAATAKRSRLLLHYTPVSHHGIAPYFTEYIRQTVKPASMLGDINLHRDGL
HHHHHCCCCHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHCCCEEECCCE
TIQTTLDSRMQKYAEQAAVDHLASLQASFDRSWRWPESLKNQMIKESPRFKELLEDGVSA
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCHH
QQALVQLRADRIWLNNLLREKTRIQVALVAIDPANGHIKAWVGGNSLSSEDYKYQFDHVW
HHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEHHHHHH
QAKRQPGSTFKPFVYLTAIDKGIPANFRVLDQPLALKSGNGIWSPRNSDGSSGGMTTLRS
HHHCCCCCCCCCEEEEEEECCCCCCCCEEECCCEEEECCCCCCCCCCCCCCCCHHHHHHH
ALTRSLNQVSVRLAYEQLTVPEIISYAKRMGISSVMPQNYSIALGTGEVSPLELAGAFST
HHHHHHHHHEEEHHHHHCCHHHHHHHHHHCCHHHCCCCCEEEEEECCCCCHHHHHHHHHH
FANNGIWTEPVSILKVSDKHHRSMSESTPNRRFAIDSTSNFVMVSMMRDVINRGTASSIR
HHCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHHHCCCCCHHHC
GRYAMTMDAAGKTGTTQSLRDAWFAGFTPQLVAVVWTGFDDERIKFTSMEYGQGARAALP
CCEEEEEECCCCCCCHHHHHHHHHCCCCHHEEEEEECCCCCCCEEEEEECCCCCCCCHHH
IWAGFMKRCYSDPSLKLTNRYFHIPNTVIAVPISGQASVASAPANNSVYIEYYTPKGFNY
HHHHHHHHHCCCCCEEEECEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCC
YKSNPVQSTPGDQGDSGVPTEGDLEPAPQPAETPAQEGAF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MITLRTTVTVNTTFFKKSFITLAALVFFLLTSVSAFALNPFKGLPSLEELENPNPDLASL
CEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHE
VYSEDGVLLHKFFVKNRTFIPLKSIPRSARYALIATEDVTFYQHWGVDLRRLALVMGENI
EECCCCEEEEHHHHCCCCEEEHHHCCCCCCEEEEEECCEEEHHHCCCHHHHHHHHHCCHH
IKGRQRWHGASTITQQLAKNLYLTQERTVSRKVKELITAVELEKTYTKDEILALYLNTVY
HHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEHHHE
FGSGAYGIEAAARTYFSKPASQLTLPESATLIATLKNPTGYNPVKNPSGAISRRNLILSL
ECCCCCHHHHHHHHHHCCCCHHEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
MEKEKFITPEQAATAKRSRLLLHYTPVSHHGIAPYFTEYIRQTVKPASMLGDINLHRDGL
HHHHHCCCCHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHCCCEEECCCE
TIQTTLDSRMQKYAEQAAVDHLASLQASFDRSWRWPESLKNQMIKESPRFKELLEDGVSA
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCHH
QQALVQLRADRIWLNNLLREKTRIQVALVAIDPANGHIKAWVGGNSLSSEDYKYQFDHVW
HHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEHHHHHH
QAKRQPGSTFKPFVYLTAIDKGIPANFRVLDQPLALKSGNGIWSPRNSDGSSGGMTTLRS
HHHCCCCCCCCCEEEEEEECCCCCCCCEEECCCEEEECCCCCCCCCCCCCCCCHHHHHHH
ALTRSLNQVSVRLAYEQLTVPEIISYAKRMGISSVMPQNYSIALGTGEVSPLELAGAFST
HHHHHHHHHEEEHHHHHCCHHHHHHHHHHCCHHHCCCCCEEEEEECCCCCHHHHHHHHHH
FANNGIWTEPVSILKVSDKHHRSMSESTPNRRFAIDSTSNFVMVSMMRDVINRGTASSIR
HHCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHHHCCCCCHHHC
GRYAMTMDAAGKTGTTQSLRDAWFAGFTPQLVAVVWTGFDDERIKFTSMEYGQGARAALP
CCEEEEEECCCCCCCHHHHHHHHHCCCCHHEEEEEECCCCCCCEEEEEECCCCCCCCHHH
IWAGFMKRCYSDPSLKLTNRYFHIPNTVIAVPISGQASVASAPANNSVYIEYYTPKGFNY
HHHHHHHHHCCCCCEEEECEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCC
YKSNPVQSTPGDQGDSGVPTEGDLEPAPQPAETPAQEGAF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA