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The map label for this gene is tusE [H]

Identifier: 119355977

GI number: 119355977

Start: 149068

End: 149403

Strand: Direct

Name: tusE [H]

Synonym: Cpha266_0127

Alternate gene names: 119355977

Gene position: 149068-149403 (Clockwise)

Preceding gene: 119355976

Following gene: 119355978

Centisome position: 4.76

GC content: 49.4

Gene sequence:

>336_bases
ATGGCAATTGTAGTTAATGGGGTCAGTTGCGAGACAGATGAGAACGGTTATCTCGTCAATCTCGAAGATTGGACAGAGGA
TGTTGCGAAGGTGCTGGCTGAAGGCGAAGAGATCGAGATGACTGAAGCTCACTGGGATCTCGTTAAATTTCTCAGGGGTT
ATTACGACGAGTACCAGATCGCTCCTGCCGTCAAGGTTCTAACAAAGGCTATTGCTGCCGAAAAGGATATGGACAAGAAG
AACGCGTCAGAGTTCCTTTACGGACTTTTCCCGAAGGGCCCGGGTCTTCAGGCCTGTAAAATTGCCGGACTGCCTAAACC
GACCGGTTGCGTTTAA

Upstream 100 bases:

>100_bases
GAGGTGCTGTTTACTGTTATTGAGCGACAGGCTGTCGTAGGCTTCGTCTGTAATAGCTCAGGTTTTATCCATTCAGATTA
AAAAAATAAAGGAGTTCGAC

Downstream 100 bases:

>100_bases
GAAAACGGAAAGCTGTTTGCTGACCCCAATTTACCGAGAATTAAAGGAGGTGCTATGGACGGTGCTGAAGGTGCTGCTTC
AAAGGAAGTACATCATTGCA

Product: DsrC family protein

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein E [H]

Number of amino acids: Translated: 111; Mature: 110

Protein sequence:

>111_residues
MAIVVNGVSCETDENGYLVNLEDWTEDVAKVLAEGEEIEMTEAHWDLVKFLRGYYDEYQIAPAVKVLTKAIAAEKDMDKK
NASEFLYGLFPKGPGLQACKIAGLPKPTGCV

Sequences:

>Translated_111_residues
MAIVVNGVSCETDENGYLVNLEDWTEDVAKVLAEGEEIEMTEAHWDLVKFLRGYYDEYQIAPAVKVLTKAIAAEKDMDKK
NASEFLYGLFPKGPGLQACKIAGLPKPTGCV
>Mature_110_residues
AIVVNGVSCETDENGYLVNLEDWTEDVAKVLAEGEEIEMTEAHWDLVKFLRGYYDEYQIAPAVKVLTKAIAAEKDMDKKN
ASEFLYGLFPKGPGLQACKIAGLPKPTGCV

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Could accept sulfur from tusD [H]

COG id: COG2920

COG function: function code P; Dissimilatory sulfite reductase (desulfoviridin), gamma subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrC/tusE family [H]

Homologues:

Organism=Escherichia coli, GI87081811, Length=109, Percent_Identity=45.8715596330275, Blast_Score=108, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007453 [H]

Pfam domain/function: PF04358 DsrC [H]

EC number: NA

Molecular weight: Translated: 12214; Mature: 12083

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVVNGVSCETDENGYLVNLEDWTEDVAKVLAEGEEIEMTEAHWDLVKFLRGYYDEYQI
CEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCH
APAVKVLTKAIAAEKDMDKKNASEFLYGLFPKGPGLQACKIAGLPKPTGCV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure 
AIVVNGVSCETDENGYLVNLEDWTEDVAKVLAEGEEIEMTEAHWDLVKFLRGYYDEYQI
EEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCH
APAVKVLTKAIAAEKDMDKKNASEFLYGLFPKGPGLQACKIAGLPKPTGCV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]