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The map label for this gene is psrB [H]

Identifier: 119355969

GI number: 119355969

Start: 139074

End: 140627

Strand: Direct

Name: psrB [H]

Synonym: Cpha266_0119

Alternate gene names: 119355969

Gene position: 139074-140627 (Clockwise)

Preceding gene: 119355967

Following gene: 119355970

Centisome position: 4.44

GC content: 53.15

Gene sequence:

>1554_bases
ATGAATTACGGTTTTGTTATCGATGCCCGCAAATGTATCGGATGTCATGCATGCACGGTTGCATGCAAGTCCGAAAATCA
GGTTCCTCTTGGAGTGAACCGTACATGGGTGAAATATGTTGAAAAGGGCTCTTTTCCTGAAACAAGGAGGTACTTTACCG
TTCTTCGCTGCAACCACTGCGCAGAGCCTCCGTGCGTGGCCATTTGTCCCGTCGAGGCTTTGCAGAAGCGCGAAGACGGT
ATTGTCGATTTTGACGGACGCCGCTGTATAGGATGTAAAGCCTGCGCACAGGCCTGCCCTTACAATGCGCTCTATATCGA
CCCGGAAACGCATACCTCCGCTAAGTGCAACTACTGCGCTCATCGCAAGGAGGTTGGCCTTCAGCCAGCCTGTGTTGCCA
TTTGTCCCCAGCAGGCAATCGTGTCGGGAGATCTTGACGATCCTTCCAGTAAAATCGCTGCGCTGGTTGCCGGAGAGCAG
ACTGTTGTGCGCAAGCCTGAAAAAGGCACTTCACCCAAACTGTTTTATATCAATGGAGACGGCGCTTCACTCGATCCTCT
TCAGGTTTCGCTTGAAAAAGAGTATCACTGGAGCGCCCAGGCGCGCGGCGTTGGGCATTTTGCCGATAAGCAGAAGGGCG
CTCCGGCAATTGAAAAACCACATCATGAGGCCCCGGCAACAGGACCAGCCATGCGTCGGGTGTACGATATTCCTTCAAAA
GGGGCTGTCTGGGGGTGGGAGGTTCCGGCATATGTTTCGGCAAAAGCCGTCTCGTCCGGCGTTTTCCTCTTGTTTTTTGC
CCTGACGGCCCTTCTTGGTCAGGAACTTTCAGGTATGATGCAGTGGGCTGCATGGGCTACCTCGCTTGTGTTTTTAGGTT
TGACCGGCGGTTTTCTTATCAAGGATCTTGATCGCCCGGACCGCTTTTCTTCGGTGATGCTCCGTCCTCAGTTCGGCTCC
TGGCTGGTAAAGGGCGGGTTGACCATTACCGGATACGGAGCGTTTCTTGCGCTTTGGGGAGCTTCACGATTCCTGCAGAT
TCCGGCTCTTGAACGCGTTGCCCTCTGGGGAGGGGCGCTTTTCGCTCTGATTACGGCAATCTATACCGCTTTTCTGTTCG
GTTCGGCAAAAGGACGCGATTTCTGGCAGAGCCCTATGCTTCTCTTACACATGTTGTTGAATGCGCTGCTGGCCGGCGGT
TCCACGATGTTGTTGCTTGGTTTCGGTACCGGCGCTTCAGTGGAGCTGTTCGCGCTTCTCAAGCAACTGCTTGCGGCCGG
ATTTTCGCTTCATCTCGTCATTATGCTGCTTGAGCTCTATGGTAAGCATCCGTCGCTACAGGCGGAACGGACGGCTGAAA
TTATCAGGTCAGGCAGTTTGAAAAACGAGTTCTGGATCGGCAGTTTCATTGCGGGAAATCTCTTGCCGCTCATTTTGTCG
ATTCTCTCATCCGATCCGCTTATGCTTGCTGCTGCAGCCATCCTGGGGCTTTGCGGTGTGTTCTATACAGAAAAAGTATG
GGTTCAGGCCCCGCAGAGAGTTCCCTTAAGTTAA

Upstream 100 bases:

>100_bases
GTTATATAACGATATTGTAAGAAGGGTTTAACGGCGTATAAAGCGTGAACCCGTAAGCATAAAACGGCGGAAGGAACGCA
ATTTTAGAGAAAATTATTCC

Downstream 100 bases:

>100_bases
CCATGTGTCGGAGTTCATTATGAGTTATACCCATAAACCAACCGTAATAGAAAGCATTGCAGAAAAACTGCACCTTATTC
CCGATCTCCATAAGGAAAAC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Sulfur reductase chain B [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MNYGFVIDARKCIGCHACTVACKSENQVPLGVNRTWVKYVEKGSFPETRRYFTVLRCNHCAEPPCVAICPVEALQKREDG
IVDFDGRRCIGCKACAQACPYNALYIDPETHTSAKCNYCAHRKEVGLQPACVAICPQQAIVSGDLDDPSSKIAALVAGEQ
TVVRKPEKGTSPKLFYINGDGASLDPLQVSLEKEYHWSAQARGVGHFADKQKGAPAIEKPHHEAPATGPAMRRVYDIPSK
GAVWGWEVPAYVSAKAVSSGVFLLFFALTALLGQELSGMMQWAAWATSLVFLGLTGGFLIKDLDRPDRFSSVMLRPQFGS
WLVKGGLTITGYGAFLALWGASRFLQIPALERVALWGGALFALITAIYTAFLFGSAKGRDFWQSPMLLLHMLLNALLAGG
STMLLLGFGTGASVELFALLKQLLAAGFSLHLVIMLLELYGKHPSLQAERTAEIIRSGSLKNEFWIGSFIAGNLLPLILS
ILSSDPLMLAAAAILGLCGVFYTEKVWVQAPQRVPLS

Sequences:

>Translated_517_residues
MNYGFVIDARKCIGCHACTVACKSENQVPLGVNRTWVKYVEKGSFPETRRYFTVLRCNHCAEPPCVAICPVEALQKREDG
IVDFDGRRCIGCKACAQACPYNALYIDPETHTSAKCNYCAHRKEVGLQPACVAICPQQAIVSGDLDDPSSKIAALVAGEQ
TVVRKPEKGTSPKLFYINGDGASLDPLQVSLEKEYHWSAQARGVGHFADKQKGAPAIEKPHHEAPATGPAMRRVYDIPSK
GAVWGWEVPAYVSAKAVSSGVFLLFFALTALLGQELSGMMQWAAWATSLVFLGLTGGFLIKDLDRPDRFSSVMLRPQFGS
WLVKGGLTITGYGAFLALWGASRFLQIPALERVALWGGALFALITAIYTAFLFGSAKGRDFWQSPMLLLHMLLNALLAGG
STMLLLGFGTGASVELFALLKQLLAAGFSLHLVIMLLELYGKHPSLQAERTAEIIRSGSLKNEFWIGSFIAGNLLPLILS
ILSSDPLMLAAAAILGLCGVFYTEKVWVQAPQRVPLS
>Mature_517_residues
MNYGFVIDARKCIGCHACTVACKSENQVPLGVNRTWVKYVEKGSFPETRRYFTVLRCNHCAEPPCVAICPVEALQKREDG
IVDFDGRRCIGCKACAQACPYNALYIDPETHTSAKCNYCAHRKEVGLQPACVAICPQQAIVSGDLDDPSSKIAALVAGEQ
TVVRKPEKGTSPKLFYINGDGASLDPLQVSLEKEYHWSAQARGVGHFADKQKGAPAIEKPHHEAPATGPAMRRVYDIPSK
GAVWGWEVPAYVSAKAVSSGVFLLFFALTALLGQELSGMMQWAAWATSLVFLGLTGGFLIKDLDRPDRFSSVMLRPQFGS
WLVKGGLTITGYGAFLALWGASRFLQIPALERVALWGGALFALITAIYTAFLFGSAKGRDFWQSPMLLLHMLLNALLAGG
STMLLLGFGTGASVELFALLKQLLAAGFSLHLVIMLLELYGKHPSLQAERTAEIIRSGSLKNEFWIGSFIAGNLLPLILS
ILSSDPLMLAAAAILGLCGVFYTEKVWVQAPQRVPLS

Specific function: Component of the phosphorylative electron transport system with polysulfide as the terminal acceptor [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=180, Percent_Identity=41.1111111111111, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI226510944, Length=151, Percent_Identity=35.7615894039735, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1787872, Length=187, Percent_Identity=33.6898395721925, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787122, Length=187, Percent_Identity=33.1550802139037, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI2367245, Length=151, Percent_Identity=35.7615894039735, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790326, Length=157, Percent_Identity=35.031847133758, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787749, Length=183, Percent_Identity=32.7868852459016, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082180, Length=145, Percent_Identity=38.6206896551724, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082179, Length=145, Percent_Identity=33.1034482758621, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1789370, Length=171, Percent_Identity=30.9941520467836, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788811, Length=150, Percent_Identity=30, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789067, Length=164, Percent_Identity=32.9268292682927, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787740, Length=121, Percent_Identity=34.7107438016529, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1787963, Length=149, Percent_Identity=28.1879194630872, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787478, Length=80, Percent_Identity=42.5, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789079, Length=107, Percent_Identity=38.3177570093458, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87082114, Length=184, Percent_Identity=28.804347826087, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 56049; Mature: 56049

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYGFVIDARKCIGCHACTVACKSENQVPLGVNRTWVKYVEKGSFPETRRYFTVLRCNHC
CCCCEEEECHHHHCCHHEEEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHEEEEEECCC
AEPPCVAICPVEALQKREDGIVDFDGRRCIGCKACAQACPYNALYIDPETHTSAKCNYCA
CCCCEEEECCHHHHHHHCCCCEECCCCEEECHHHHHHHCCCCEEEECCCCCCCCCCHHHH
HRKEVGLQPACVAICPQQAIVSGDLDDPSSKIAALVAGEQTVVRKPEKGTSPKLFYINGD
HHHHCCCCCHHEEECCCHHHEECCCCCCHHHEEEEEECCHHEEECCCCCCCCEEEEEECC
GASLDPLQVSLEKEYHWSAQARGVGHFADKQKGAPAIEKPHHEAPATGPAMRRVYDIPSK
CCCCCCEEEEEECCCCCCHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
GAVWGWEVPAYVSAKAVSSGVFLLFFALTALLGQELSGMMQWAAWATSLVFLGLTGGFLI
CCEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
KDLDRPDRFSSVMLRPQFGSWLVKGGLTITGYGAFLALWGASRFLQIPALERVALWGGAL
ECCCCCCHHHHEEECCCCCCEEEECCEEEEEHHHHHHHHHCHHEEECCCHHHHHHHHHHH
FALITAIYTAFLFGSAKGRDFWQSPMLLLHMLLNALLAGGSTMLLLGFGTGASVELFALL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
KQLLAAGFSLHLVIMLLELYGKHPSLQAERTAEIIRSGSLKNEFWIGSFIAGNLLPLILS
HHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHH
ILSSDPLMLAAAAILGLCGVFYTEKVWVQAPQRVPLS
HHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
>Mature Secondary Structure
MNYGFVIDARKCIGCHACTVACKSENQVPLGVNRTWVKYVEKGSFPETRRYFTVLRCNHC
CCCCEEEECHHHHCCHHEEEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHEEEEEECCC
AEPPCVAICPVEALQKREDGIVDFDGRRCIGCKACAQACPYNALYIDPETHTSAKCNYCA
CCCCEEEECCHHHHHHHCCCCEECCCCEEECHHHHHHHCCCCEEEECCCCCCCCCCHHHH
HRKEVGLQPACVAICPQQAIVSGDLDDPSSKIAALVAGEQTVVRKPEKGTSPKLFYINGD
HHHHCCCCCHHEEECCCHHHEECCCCCCHHHEEEEEECCHHEEECCCCCCCCEEEEEECC
GASLDPLQVSLEKEYHWSAQARGVGHFADKQKGAPAIEKPHHEAPATGPAMRRVYDIPSK
CCCCCCEEEEEECCCCCCHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
GAVWGWEVPAYVSAKAVSSGVFLLFFALTALLGQELSGMMQWAAWATSLVFLGLTGGFLI
CCEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
KDLDRPDRFSSVMLRPQFGSWLVKGGLTITGYGAFLALWGASRFLQIPALERVALWGGAL
ECCCCCCHHHHEEECCCCCCEEEECCEEEEEHHHHHHHHHCHHEEECCCHHHHHHHHHHH
FALITAIYTAFLFGSAKGRDFWQSPMLLLHMLLNALLAGGSTMLLLGFGTGASVELFALL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
KQLLAAGFSLHLVIMLLELYGKHPSLQAERTAEIIRSGSLKNEFWIGSFIAGNLLPLILS
HHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHH
ILSSDPLMLAAAAILGLCGVFYTEKVWVQAPQRVPLS
HHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1597189; 14500908 [H]