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The map label for this gene is bioB [H]

Identifier: 119355967

GI number: 119355967

Start: 136426

End: 137730

Strand: Direct

Name: bioB [H]

Synonym: Cpha266_0117

Alternate gene names: 119355967

Gene position: 136426-137730 (Clockwise)

Preceding gene: 119355966

Following gene: 119355969

Centisome position: 4.35

GC content: 55.63

Gene sequence:

>1305_bases
ATGACCTTGCCTTTGCCAGTTATAGATCTTGCTTTTGACCGGAGCCATATCTGGCACCCGTACACCTCGCTTGCCGATCC
CCTTCCTGTCTATCCCGTAACCGGAGCGCGGGGGGTCTATCTGGAGCTTGCCGACGGACGGAGACTTGTTGACGGGATGT
CATCATGGTGGGCGGCCATTCATGGTTACAATCATCCGGTTCTTAATCAGGCGGTAACGACGCAGCTTGAGCGGATGAGC
CATGTGATGTTTGGCGGTCTGACGCATGAGCCAGCCATAACGCTTGCCGAACAGCTTGCAGAGCTGACTCCGGAACCGCT
GCAGAAGGTGTTTTTCAGCGATTCGGGATCGGTTTCCGTTGAGGTCGCTATCAAAATGGCGATCCAGTACTGGGCTACGC
TCGGAGAGACCCGGAAAACCAGACTTCTGACCGTTCGTTCCGGCTATCATGGCGATACGTTTGCCGCCATGTCGGTTTCC
GACCCTGTTACCGGCATGCATCGTCTTTTCAGCGGCGTCCTGCAGCGGCAGATCTTTGCCGAATCACCCTCATGCGCTTT
TCACGATCCATGGAACGAGGAGTGTATCGGAGATCTCCGCCGGAAACTGGAGCGCCATGCCGATGAAATTGCCGCAGTCA
TTATCGAGCCTGTCGTTCAGGGTGCGGGAGGGATGCGGTTTTATGCTCCCCGATATCTCGGTCGTCTCAGGGAACTGTGC
GACGAGTTCGGCGTGTTGCTGATATTCGATGAAATCGCAACAGGGTTCGGACGCAGCGGCAAGCTGTTTGCGCTTGAGCA
TGCAGAGGTTTCTCCTGATATACTCTGTGTCGGCAAGGCGCTTACCGGCGGCTACATGACGCTTGCAGCCACCCTGACGA
CGGGTGTCGTAGCGGAGACGATTTCATCCGGCAACCCCGGGCTTTTCATGCACGGCCCGACCTTTATGGCCAATCCGCTT
GCCTGCGCAGTTGCGGCAGCCAATATCCGTCTTCTTCTCAGTTATGACTGGAAAAGCAAGGTTGCGGGTATTGAGCGAGG
GCTTGAATCGGCACTTTCGCCATGCAGGACAATGGAAGGAGTCGTTGATGTCCGGGTGCTTGGCGCAATAGGGGTTGTCG
AGCTGGAAAATCCTGTTGAGATGGGCCTCATCCAGAAGAAGTTCGTCGACAGAGGGGTCTGGGTAAGACCTTTCGGGAGG
CTGGTTTATCTTATGCCGCCTTATATCATTACCGACGAGGAGCTCGGTCTGCTTGCCGATGCGGTTACGGAGGTTGTTTA
TGAAACTCTTGCGGAGAGCCGGTAA

Upstream 100 bases:

>100_bases
ACGGGTTACGCGCGCCGCTGTTCACGCTTGTCGGCGATACGTTGGATTGCGGGTGTATCGAGTCACTTTTTTTATGATCA
ATTTAACCCGATAAGGATCA

Downstream 100 bases:

>100_bases
GGAGCTGAATTCCCGGAGAAAATGTAAGCATGGTCGCCACAAATCAATGAAAAAAAGTGCTGAGTTCTGAGTGCCGAGTT
GGGGGTACAGGGTAATGGGT

Product: aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTLPLPVIDLAFDRSHIWHPYTSLADPLPVYPVTGARGVYLELADGRRLVDGMSSWWAAIHGYNHPVLNQAVTTQLERMS
HVMFGGLTHEPAITLAEQLAELTPEPLQKVFFSDSGSVSVEVAIKMAIQYWATLGETRKTRLLTVRSGYHGDTFAAMSVS
DPVTGMHRLFSGVLQRQIFAESPSCAFHDPWNEECIGDLRRKLERHADEIAAVIIEPVVQGAGGMRFYAPRYLGRLRELC
DEFGVLLIFDEIATGFGRSGKLFALEHAEVSPDILCVGKALTGGYMTLAATLTTGVVAETISSGNPGLFMHGPTFMANPL
ACAVAAANIRLLLSYDWKSKVAGIERGLESALSPCRTMEGVVDVRVLGAIGVVELENPVEMGLIQKKFVDRGVWVRPFGR
LVYLMPPYIITDEELGLLADAVTEVVYETLAESR

Sequences:

>Translated_434_residues
MTLPLPVIDLAFDRSHIWHPYTSLADPLPVYPVTGARGVYLELADGRRLVDGMSSWWAAIHGYNHPVLNQAVTTQLERMS
HVMFGGLTHEPAITLAEQLAELTPEPLQKVFFSDSGSVSVEVAIKMAIQYWATLGETRKTRLLTVRSGYHGDTFAAMSVS
DPVTGMHRLFSGVLQRQIFAESPSCAFHDPWNEECIGDLRRKLERHADEIAAVIIEPVVQGAGGMRFYAPRYLGRLRELC
DEFGVLLIFDEIATGFGRSGKLFALEHAEVSPDILCVGKALTGGYMTLAATLTTGVVAETISSGNPGLFMHGPTFMANPL
ACAVAAANIRLLLSYDWKSKVAGIERGLESALSPCRTMEGVVDVRVLGAIGVVELENPVEMGLIQKKFVDRGVWVRPFGR
LVYLMPPYIITDEELGLLADAVTEVVYETLAESR
>Mature_433_residues
TLPLPVIDLAFDRSHIWHPYTSLADPLPVYPVTGARGVYLELADGRRLVDGMSSWWAAIHGYNHPVLNQAVTTQLERMSH
VMFGGLTHEPAITLAEQLAELTPEPLQKVFFSDSGSVSVEVAIKMAIQYWATLGETRKTRLLTVRSGYHGDTFAAMSVSD
PVTGMHRLFSGVLQRQIFAESPSCAFHDPWNEECIGDLRRKLERHADEIAAVIIEPVVQGAGGMRFYAPRYLGRLRELCD
EFGVLLIFDEIATGFGRSGKLFALEHAEVSPDILCVGKALTGGYMTLAATLTTGVVAETISSGNPGLFMHGPTFMANPLA
CAVAAANIRLLLSYDWKSKVAGIERGLESALSPCRTMEGVVDVRVLGAIGVVELENPVEMGLIQKKFVDRGVWVRPFGRL
VYLMPPYIITDEELGLLADAVTEVVYETLAESR

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=423, Percent_Identity=28.6052009456265, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI13994255, Length=319, Percent_Identity=28.5266457680251, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI284507298, Length=310, Percent_Identity=29.0322580645161, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI37574042, Length=433, Percent_Identity=22.4018475750577, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI226442709, Length=396, Percent_Identity=22.7272727272727, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI226442705, Length=433, Percent_Identity=22.1709006928406, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1786991, Length=420, Percent_Identity=62.3809523809524, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI145693181, Length=300, Percent_Identity=37, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1789759, Length=311, Percent_Identity=36.0128617363344, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1789016, Length=389, Percent_Identity=28.7917737789203, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1788044, Length=393, Percent_Identity=30.7888040712468, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1787560, Length=420, Percent_Identity=26.9047619047619, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1786349, Length=300, Percent_Identity=32, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=432, Percent_Identity=26.8518518518519, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25144274, Length=223, Percent_Identity=32.2869955156951, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI32564660, Length=438, Percent_Identity=25.1141552511416, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17541228, Length=288, Percent_Identity=26.3888888888889, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324386, Length=459, Percent_Identity=46.4052287581699, Blast_Score=420, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6323470, Length=417, Percent_Identity=25.6594724220624, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6324432, Length=311, Percent_Identity=27.9742765273312, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321456, Length=325, Percent_Identity=24.9230769230769, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21357415, Length=419, Percent_Identity=25.7756563245823, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI28574759, Length=431, Percent_Identity=26.2180974477958, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI161085790, Length=430, Percent_Identity=25.3488372093023, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21356575, Length=440, Percent_Identity=25.4545454545455, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281366494, Length=287, Percent_Identity=26.8292682926829, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24667139, Length=287, Percent_Identity=26.8292682926829, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24667143, Length=287, Percent_Identity=26.8292682926829, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR007197 [H]

Pfam domain/function: PF00202 Aminotran_3; PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 47548; Mature: 47417

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPLPVIDLAFDRSHIWHPYTSLADPLPVYPVTGARGVYLELADGRRLVDGMSSWWAAI
CCCCCCHHHEEECCCCCCCCHHHHCCCCCCEECCCCCEEEEEECCCCHHHHHHHHHHHHH
HGYNHPVLNQAVTTQLERMSHVMFGGLTHEPAITLAEQLAELTPEPLQKVFFSDSGSVSV
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCEEE
EVAIKMAIQYWATLGETRKTRLLTVRSGYHGDTFAAMSVSDPVTGMHRLFSGVLQRQIFA
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
ESPSCAFHDPWNEECIGDLRRKLERHADEIAAVIIEPVVQGAGGMRFYAPRYLGRLRELC
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
DEFGVLLIFDEIATGFGRSGKLFALEHAEVSPDILCVGKALTGGYMTLAATLTTGVVAET
HHCCEEEEEHHHHHCCCCCCCEEEEECCCCCCCEEEECCHHCCCHHHHHHHHHHHHHHHH
ISSGNPGLFMHGPTFMANPLACAVAAANIRLLLSYDWKSKVAGIERGLESALSPCRTMEG
HCCCCCCEEEECCCHHCCHHHHHHHHCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
VVDVRVLGAIGVVELENPVEMGLIQKKFVDRGVWVRPFGRLVYLMPPYIITDEELGLLAD
HHHEEECCCCCEEEECCCHHHHHHHHHHHHCCCEECCCCCEEEECCCEEECCCHHHHHHH
AVTEVVYETLAESR
HHHHHHHHHHHCCC
>Mature Secondary Structure 
TLPLPVIDLAFDRSHIWHPYTSLADPLPVYPVTGARGVYLELADGRRLVDGMSSWWAAI
CCCCCHHHEEECCCCCCCCHHHHCCCCCCEECCCCCEEEEEECCCCHHHHHHHHHHHHH
HGYNHPVLNQAVTTQLERMSHVMFGGLTHEPAITLAEQLAELTPEPLQKVFFSDSGSVSV
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCEEE
EVAIKMAIQYWATLGETRKTRLLTVRSGYHGDTFAAMSVSDPVTGMHRLFSGVLQRQIFA
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
ESPSCAFHDPWNEECIGDLRRKLERHADEIAAVIIEPVVQGAGGMRFYAPRYLGRLRELC
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
DEFGVLLIFDEIATGFGRSGKLFALEHAEVSPDILCVGKALTGGYMTLAATLTTGVVAET
HHCCEEEEEHHHHHCCCCCCCEEEEECCCCCCCEEEECCHHCCCHHHHHHHHHHHHHHHH
ISSGNPGLFMHGPTFMANPLACAVAAANIRLLLSYDWKSKVAGIERGLESALSPCRTMEG
HCCCCCCEEEECCCHHCCHHHHHHHHCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
VVDVRVLGAIGVVELENPVEMGLIQKKFVDRGVWVRPFGRLVYLMPPYIITDEELGLLAD
HHHEEECCCCCEEEECCCHHHHHHHHHHHHCCCEECCCCCEEEECCCEEECCCHHHHHHH
AVTEVVYETLAESR
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA