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The map label for this gene is topA [H]

Identifier: 119355959

GI number: 119355959

Start: 126637

End: 129036

Strand: Reverse

Name: topA [H]

Synonym: Cpha266_0109

Alternate gene names: 119355959

Gene position: 129036-126637 (Counterclockwise)

Preceding gene: 119355968

Following gene: 119355953

Centisome position: 4.12

GC content: 51.71

Gene sequence:

>2400_bases
ATGGCTTTAAAATCCCCGGCAGCCTCGGCAAGAAACAGGACTCTTATTGTTGTCGAGTCTCCATCAAAGGCAAAAACAAT
CAACAAATACCTTGGGGACAAATATACCGTTTTCGCTTCGGTCGGGCACATCAAGGATCTTCCGAAAAAAGAGATCGGAC
TTGATTTCGATCACCACTATGAACCACGATACGAAGTCATTGCAGGAAAAGAAAAAGTTGTCCGGCAGCTCAAAAAGCTT
GCCGATGAATCCAACGAGATCCTGATCGCAACTGACCCTGACCGCGAAGGCGAAGCGATTGCCTGGCATATTGCCAACGA
AGTGGAATTTGCCCATAAACCGGTATTCCGGGTGCTGTTCAATGAAATCACCAAAACAGCCATTATCGCCGCTATCCAGC
AACCTCGCCAAATCGACTACCGCCTTGTCCGCTCCCAGCAGACGCGACAGGGTCTCGATAAAATCGTAGGATACAAGATC
AGCCCCTTTCTATGGAATGTTGTCTTGCGCGGACTCTCCGCAGGAAGAGTTCAGTCTGTCGCATTACGGTTGATCTGTGA
ACGCGAAGCCGAAATCAATGGATTTCAGATCCAGGAGTACTGGAGCATTGCCGCTGACTTTAAAACCGATCTCAACGAAA
CCTTCCGGACAAAGCTCATTAAAATAAAGGGCGACAAACCCGAGCTTTCCAATCAGAAAAAAGCCGAAACCGCTGCGGCA
GAAATCAGTGAACGCCTGTTTGCCGTAAAGGAGATAACGACAAGGGTGCAACAGCGAAAGCCCCCGTTTCCCTTTACCAC
CTCGCTTCTTCAGCAGGCGGCCTCAAACCAGCTCGGGTTCGGATCGCAGAAGACCATGCGGATTGCCCAGCAGCTCTATG
AAGGGATCGAGCTTGGCGACGAAGGCGCAACCGGTCTGATCACCTATATGAGAACCGACTCAATCAGAATAGGAACAGAA
GCGACCGCCGAGGCAAGAAACTATATCACCGCTCAATTCGGCAAGGAGTACGTCGGATACGGAGGCGCTGCAAAAGCGGG
CAAAAACGCGCAGGACGCCCATGAGGCAATCCGTCCCACCTCGCTGTTACGAAAACCGGAATCGGTCAAACACTTCCTTT
CAAGCGATCAGTTCCGACTGTACGAACTGATCTGGAGGCGGCTCCTTACCGCCATGATGGCTCCCGCAAAAATAGAACAG
ACAAGAGTCGATGTCGAAGATCGTGAAAAAGAGTTCACCTTCAGAGCCAATGGCAGCAGGGTGCTTTTTGCCGGATTCAT
GCGGGTTTATGACGATCAGCAGGAACTGGATTATGAAGCTCAGTCCTCAACGCGCGACGATGTTGAAAAAGAACAGACTG
TTCAGCTCCCCGAGAAACTGAAGGAACAGGACAAGCTTGGTCTCGCAGGCCTTGACAGCAAGCAGAGCTTCACCCGACCT
CCCGCCCGATACAGCGAAGCAACGCTCGTCAAGGATCTGGACAACTACGGGATCGGACGCCCGTCAACCTATGCATCGAT
TTTTTCAACCCTGCAGGATCGACGCTATGTTGAGCTGCAAAAGAAAAAAATCATACCGACAGAGCTTGGCAAGGATGTCT
CGCTTATTCTTGTGGCAAATTTTCCCGAACTGTTCAATGTCGGCTTCACGGCACATATGGAGGACGAGCTTGACAAAGTT
GCTTCCGGAGACGATGAGTACGAAGCTGTGCTCGAAAAATTCTATCGACCGCTTGAAACGGCATTGAGTCTGAGAAAAAG
CGACCCGATCATTCCCCAGAACAGTGCGACCGAGACCTGCGACAAATGCGGAAAGGGACAAATGACCGTAAAATGGACGG
CAAGCGGAAAATTTCTCGGCTGTTCGCGCTACCCTTCATGCAAAAACATCAAGCCGCTGAGCTCATCGAAAGCAAAACCG
AAAGATACCGGAATACAGTGTCCCTCCTGTAAAGAGGGACATATGCTGCTCCGAGACGGAAGACTCGGCCCTTTTCTTGC
CTGCTCCAGCTATCCGAAATGCAATACGCTGCTCAATCTCGGCAAACAGCGCCAGGTCGAACCGATGAAAATTCCTCCGC
TCCTCACCACCCTTGTCTGCCCGAAATGCGGAGCTCCAATGAACCTCAGAAGCGGAAAAAGAGGACTATGGCTCGGATGC
TCCAAGTTCCCGAAATGCCGCGGACGACTTGCCTGGGGAACCCTGGATGAGGAGGTTCAGCGCCACTGGGAAACCCTGAT
GACAGAGCATCAAAAAGCCCATCCGACAGTTGCGCTGACCATGATAGACGGGCGTCCGGTACCGCTCACCCTTTCGGTTG
ACGATATCATCACCCGAGCCGAAGAGGCCGGACTTATTGCCGCCGTACCGGAAGAGCAGCAGGAGCTCACAGCAGAATAA

Upstream 100 bases:

>100_bases
AAAGATCGCCGTACCGGAAATCGCCGAAAATTTGTTTTTTCCCGAGCAACACCTATCTTTTTGCAAACTTTATCCTAACG
CTCTTACATCGGAACTGACA

Downstream 100 bases:

>100_bases
GTGCCAGCCGGTAGGTAACGAACGAGAGTATCCACGAAACCGCAAGCACATACACGGAATAGAGCAAAACGGGTTTCCAT
CGTCCGATCTCTTTTTTCAT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 799; Mature: 798

Protein sequence:

>799_residues
MALKSPAASARNRTLIVVESPSKAKTINKYLGDKYTVFASVGHIKDLPKKEIGLDFDHHYEPRYEVIAGKEKVVRQLKKL
ADESNEILIATDPDREGEAIAWHIANEVEFAHKPVFRVLFNEITKTAIIAAIQQPRQIDYRLVRSQQTRQGLDKIVGYKI
SPFLWNVVLRGLSAGRVQSVALRLICEREAEINGFQIQEYWSIAADFKTDLNETFRTKLIKIKGDKPELSNQKKAETAAA
EISERLFAVKEITTRVQQRKPPFPFTTSLLQQAASNQLGFGSQKTMRIAQQLYEGIELGDEGATGLITYMRTDSIRIGTE
ATAEARNYITAQFGKEYVGYGGAAKAGKNAQDAHEAIRPTSLLRKPESVKHFLSSDQFRLYELIWRRLLTAMMAPAKIEQ
TRVDVEDREKEFTFRANGSRVLFAGFMRVYDDQQELDYEAQSSTRDDVEKEQTVQLPEKLKEQDKLGLAGLDSKQSFTRP
PARYSEATLVKDLDNYGIGRPSTYASIFSTLQDRRYVELQKKKIIPTELGKDVSLILVANFPELFNVGFTAHMEDELDKV
ASGDDEYEAVLEKFYRPLETALSLRKSDPIIPQNSATETCDKCGKGQMTVKWTASGKFLGCSRYPSCKNIKPLSSSKAKP
KDTGIQCPSCKEGHMLLRDGRLGPFLACSSYPKCNTLLNLGKQRQVEPMKIPPLLTTLVCPKCGAPMNLRSGKRGLWLGC
SKFPKCRGRLAWGTLDEEVQRHWETLMTEHQKAHPTVALTMIDGRPVPLTLSVDDIITRAEEAGLIAAVPEEQQELTAE

Sequences:

>Translated_799_residues
MALKSPAASARNRTLIVVESPSKAKTINKYLGDKYTVFASVGHIKDLPKKEIGLDFDHHYEPRYEVIAGKEKVVRQLKKL
ADESNEILIATDPDREGEAIAWHIANEVEFAHKPVFRVLFNEITKTAIIAAIQQPRQIDYRLVRSQQTRQGLDKIVGYKI
SPFLWNVVLRGLSAGRVQSVALRLICEREAEINGFQIQEYWSIAADFKTDLNETFRTKLIKIKGDKPELSNQKKAETAAA
EISERLFAVKEITTRVQQRKPPFPFTTSLLQQAASNQLGFGSQKTMRIAQQLYEGIELGDEGATGLITYMRTDSIRIGTE
ATAEARNYITAQFGKEYVGYGGAAKAGKNAQDAHEAIRPTSLLRKPESVKHFLSSDQFRLYELIWRRLLTAMMAPAKIEQ
TRVDVEDREKEFTFRANGSRVLFAGFMRVYDDQQELDYEAQSSTRDDVEKEQTVQLPEKLKEQDKLGLAGLDSKQSFTRP
PARYSEATLVKDLDNYGIGRPSTYASIFSTLQDRRYVELQKKKIIPTELGKDVSLILVANFPELFNVGFTAHMEDELDKV
ASGDDEYEAVLEKFYRPLETALSLRKSDPIIPQNSATETCDKCGKGQMTVKWTASGKFLGCSRYPSCKNIKPLSSSKAKP
KDTGIQCPSCKEGHMLLRDGRLGPFLACSSYPKCNTLLNLGKQRQVEPMKIPPLLTTLVCPKCGAPMNLRSGKRGLWLGC
SKFPKCRGRLAWGTLDEEVQRHWETLMTEHQKAHPTVALTMIDGRPVPLTLSVDDIITRAEEAGLIAAVPEEQQELTAE
>Mature_798_residues
ALKSPAASARNRTLIVVESPSKAKTINKYLGDKYTVFASVGHIKDLPKKEIGLDFDHHYEPRYEVIAGKEKVVRQLKKLA
DESNEILIATDPDREGEAIAWHIANEVEFAHKPVFRVLFNEITKTAIIAAIQQPRQIDYRLVRSQQTRQGLDKIVGYKIS
PFLWNVVLRGLSAGRVQSVALRLICEREAEINGFQIQEYWSIAADFKTDLNETFRTKLIKIKGDKPELSNQKKAETAAAE
ISERLFAVKEITTRVQQRKPPFPFTTSLLQQAASNQLGFGSQKTMRIAQQLYEGIELGDEGATGLITYMRTDSIRIGTEA
TAEARNYITAQFGKEYVGYGGAAKAGKNAQDAHEAIRPTSLLRKPESVKHFLSSDQFRLYELIWRRLLTAMMAPAKIEQT
RVDVEDREKEFTFRANGSRVLFAGFMRVYDDQQELDYEAQSSTRDDVEKEQTVQLPEKLKEQDKLGLAGLDSKQSFTRPP
ARYSEATLVKDLDNYGIGRPSTYASIFSTLQDRRYVELQKKKIIPTELGKDVSLILVANFPELFNVGFTAHMEDELDKVA
SGDDEYEAVLEKFYRPLETALSLRKSDPIIPQNSATETCDKCGKGQMTVKWTASGKFLGCSRYPSCKNIKPLSSSKAKPK
DTGIQCPSCKEGHMLLRDGRLGPFLACSSYPKCNTLLNLGKQRQVEPMKIPPLLTTLVCPKCGAPMNLRSGKRGLWLGCS
KFPKCRGRLAWGTLDEEVQRHWETLMTEHQKAHPTVALTMIDGRPVPLTLSVDDIITRAEEAGLIAAVPEEQQELTAE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=623, Percent_Identity=22.9534510433387, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI4507635, Length=706, Percent_Identity=22.6628895184136, Blast_Score=109, Evalue=1e-23,
Organism=Escherichia coli, GI1787529, Length=818, Percent_Identity=36.5525672371638, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1788061, Length=646, Percent_Identity=23.5294117647059, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI48994931, Length=135, Percent_Identity=32.5925925925926, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17555378, Length=544, Percent_Identity=23.7132352941176, Blast_Score=117, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32563869, Length=536, Percent_Identity=24.4402985074627, Blast_Score=107, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=624, Percent_Identity=23.2371794871795, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585251, Length=670, Percent_Identity=23.7313432835821, Blast_Score=126, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 89768; Mature: 89637

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKSPAASARNRTLIVVESPSKAKTINKYLGDKYTVFASVGHIKDLPKKEIGLDFDHHY
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEECCHHHCCCHHHHCCCCCCCC
EPRYEVIAGKEKVVRQLKKLADESNEILIATDPDREGEAIAWHIANEVEFAHKPVFRVLF
CCCHHEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHH
NEITKTAIIAAIQQPRQIDYRLVRSQQTRQGLDKIVGYKISPFLWNVVLRGLSAGRVQSV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHH
ALRLICEREAEINGFQIQEYWSIAADFKTDLNETFRTKLIKIKGDKPELSNQKKAETAAA
HHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHH
EISERLFAVKEITTRVQQRKPPFPFTTSLLQQAASNQLGFGSQKTMRIAQQLYEGIELGD
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
EGATGLITYMRTDSIRIGTEATAEARNYITAQFGKEYVGYGGAAKAGKNAQDAHEAIRPT
CCCCEEEEEEECCCEEECCCHHHHHHHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHCHH
SLLRKPESVKHFLSSDQFRLYELIWRRLLTAMMAPAKIEQTRVDVEDREKEFTFRANGSR
HHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCEEEECCCCE
VLFAGFMRVYDDQQELDYEAQSSTRDDVEKEQTVQLPEKLKEQDKLGLAGLDSKQSFTRP
EHHHHHHHHHCCHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PARYSEATLVKDLDNYGIGRPSTYASIFSTLQDRRYVELQKKKIIPTELGKDVSLILVAN
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEEC
FPELFNVGFTAHMEDELDKVASGDDEYEAVLEKFYRPLETALSLRKSDPIIPQNSATETC
CHHHHHCCCEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
DKCGKGQMTVKWTASGKFLGCSRYPSCKNIKPLSSSKAKPKDTGIQCPSCKEGHMLLRDG
HHCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECC
RLGPFLACSSYPKCNTLLNLGKQRQVEPMKIPPLLTTLVCPKCGAPMNLRSGKRGLWLGC
CCCCEEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECC
SKFPKCRGRLAWGTLDEEVQRHWETLMTEHQKAHPTVALTMIDGRPVPLTLSVDDIITRA
CCCCHHCCEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEHHHHHHHH
EEAGLIAAVPEEQQELTAE
HHCCEEEECCCHHHHHCCC
>Mature Secondary Structure 
ALKSPAASARNRTLIVVESPSKAKTINKYLGDKYTVFASVGHIKDLPKKEIGLDFDHHY
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEECCHHHCCCHHHHCCCCCCCC
EPRYEVIAGKEKVVRQLKKLADESNEILIATDPDREGEAIAWHIANEVEFAHKPVFRVLF
CCCHHEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHH
NEITKTAIIAAIQQPRQIDYRLVRSQQTRQGLDKIVGYKISPFLWNVVLRGLSAGRVQSV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHH
ALRLICEREAEINGFQIQEYWSIAADFKTDLNETFRTKLIKIKGDKPELSNQKKAETAAA
HHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHH
EISERLFAVKEITTRVQQRKPPFPFTTSLLQQAASNQLGFGSQKTMRIAQQLYEGIELGD
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
EGATGLITYMRTDSIRIGTEATAEARNYITAQFGKEYVGYGGAAKAGKNAQDAHEAIRPT
CCCCEEEEEEECCCEEECCCHHHHHHHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHCHH
SLLRKPESVKHFLSSDQFRLYELIWRRLLTAMMAPAKIEQTRVDVEDREKEFTFRANGSR
HHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCEEEECCCCE
VLFAGFMRVYDDQQELDYEAQSSTRDDVEKEQTVQLPEKLKEQDKLGLAGLDSKQSFTRP
EHHHHHHHHHCCHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PARYSEATLVKDLDNYGIGRPSTYASIFSTLQDRRYVELQKKKIIPTELGKDVSLILVAN
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEEC
FPELFNVGFTAHMEDELDKVASGDDEYEAVLEKFYRPLETALSLRKSDPIIPQNSATETC
CHHHHHCCCEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
DKCGKGQMTVKWTASGKFLGCSRYPSCKNIKPLSSSKAKPKDTGIQCPSCKEGHMLLRDG
HHCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECC
RLGPFLACSSYPKCNTLLNLGKQRQVEPMKIPPLLTTLVCPKCGAPMNLRSGKRGLWLGC
CCCCEEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECC
SKFPKCRGRLAWGTLDEEVQRHWETLMTEHQKAHPTVALTMIDGRPVPLTLSVDDIITRA
CCCCHHCCEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEHHHHHHHH
EEAGLIAAVPEEQQELTAE
HHCCEEEECCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]