Click here to switch to the map view.

The map label for this gene is pqqE [H]

Identifier: 119355905

GI number: 119355905

Start: 59403

End: 60692

Strand: Reverse

Name: pqqE [H]

Synonym: Cpha266_0055

Alternate gene names: 119355905

Gene position: 60692-59403 (Counterclockwise)

Preceding gene: 119355906

Following gene: 119355904

Centisome position: 1.94

GC content: 49.92

Gene sequence:

>1290_bases
ATGCATTATCCCTATTTCCCGTTTTTTCTCAGCAAGCAGGATCGCGATACATTCATGGCCTCATCCCCCTATGGCGGGTT
CACTGTGAATAAAGCCGGTGCAGCCATTCTTGAGTGGAGCAACGGAAGCCGCTCAATGGGTGAGATCGTAAAAAAGCTGC
AAAGTCGTTATGTTGCTGAAAGCGCGTCGATTGCCGCAAAAACCGAACAATTTATCCATGATCTTGCCAAAAAGGGCTAT
GTCCTGATAAAAGAAAAACCCATGCGCTGGTTTGATGCGCCTCCTCCTCAAACCATATTCTGGGAGATTACCTCTCAATG
CAATCTGCAATGCCTTCACTGTGTCGTCTCTGCCGGACAATCGACGGAGCCCGATCTTTCAACAGCCCGTTGTTTTGAAT
TACTTGATGAATGGGCCGCACTCGGTGTAGAGGAAATAACCTTCAGCGGAGGAGAGCCGCTGCTCAGGGAAGATTTTTTC
GATCTGGCCGCTGCCGCTGCGAAAAACAACCTCAGCATCAGCCTTGCAACCAACGGCACCCTTGTTACCAGAGAGGTGGC
AAAAAAGCTTAAGGCCCTCAATGTTGATGTGCAGATCAGCCTCGACGGATCGACAGCAGAAATTTACGGACGGGTACGCG
GTCGTAAAGAAGCTTTCAATGATGTGGTACAGGGAATCAGGAATACGCTTGCCGCGGGAGTTAACCTTACCATAGGAACC
GTTGTGACAAAAAACAATATTGATGACATACCCGAAATCCTGAAGTTTACAGAGCGTTCCGGCGTCCCTTATTTCCGTCT
GATTCCCTTTGTGCCTTCCGGCCGAGGTAAACATAACCGGGATCTTGAACTCGATCCGCTCCAGATACAAACAGTTTCAA
GAGAACTTGTCGAACAGCGGGATCGCTGGTCGTTTACCATTCTTCCTCTGGAATTTGAGCTGACCTTTTCGCCCCCGTCA
GGTGAACAGGCCGATCTTTCAAGGCCAAGCGAATGCGGAGGAGCCACCAATTACTGCACCGTCACTCCATCGGGAGAGGT
ACTGCCCTGTCATTATTTTGAAGGGGTCGTTGCCGATTCATTGCGAAACCGGTCATTCCGCGATATCTGGAGAAACTCCC
GCTTTCTCAACTATTTCCGCAGTATCGAAATTGGCGATATCCAGGGGTATTGCCATGGGTGCGACTGGCTCTCCGAATGC
CGGGGGGGATGCAAAGCAGCCAATTTCAGCCGTGGCGATCTCTTTCAATCAAACTGCCACTGCTGGGTTGTCAAAGAAAA
TCACCAATAA

Upstream 100 bases:

>100_bases
TTTCAATCCTGAATGTTCGGAGTAAACCGCTCAAGTATTAATCATCAACCATGGCAGTATCGCGCAACAAGCCGATACTG
CCGTGATCTACCATACATCC

Downstream 100 bases:

>100_bases
ATAAATCATGATCGTTTCTTACATCCTCTCGCTCTCCGAGCCGCTGTCACTTCCGTCAACAGCTCTCCTCCGTATCGAAA
TCAGGGATACCTCGCTGGCA

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MHYPYFPFFLSKQDRDTFMASSPYGGFTVNKAGAAILEWSNGSRSMGEIVKKLQSRYVAESASIAAKTEQFIHDLAKKGY
VLIKEKPMRWFDAPPPQTIFWEITSQCNLQCLHCVVSAGQSTEPDLSTARCFELLDEWAALGVEEITFSGGEPLLREDFF
DLAAAAAKNNLSISLATNGTLVTREVAKKLKALNVDVQISLDGSTAEIYGRVRGRKEAFNDVVQGIRNTLAAGVNLTIGT
VVTKNNIDDIPEILKFTERSGVPYFRLIPFVPSGRGKHNRDLELDPLQIQTVSRELVEQRDRWSFTILPLEFELTFSPPS
GEQADLSRPSECGGATNYCTVTPSGEVLPCHYFEGVVADSLRNRSFRDIWRNSRFLNYFRSIEIGDIQGYCHGCDWLSEC
RGGCKAANFSRGDLFQSNCHCWVVKENHQ

Sequences:

>Translated_429_residues
MHYPYFPFFLSKQDRDTFMASSPYGGFTVNKAGAAILEWSNGSRSMGEIVKKLQSRYVAESASIAAKTEQFIHDLAKKGY
VLIKEKPMRWFDAPPPQTIFWEITSQCNLQCLHCVVSAGQSTEPDLSTARCFELLDEWAALGVEEITFSGGEPLLREDFF
DLAAAAAKNNLSISLATNGTLVTREVAKKLKALNVDVQISLDGSTAEIYGRVRGRKEAFNDVVQGIRNTLAAGVNLTIGT
VVTKNNIDDIPEILKFTERSGVPYFRLIPFVPSGRGKHNRDLELDPLQIQTVSRELVEQRDRWSFTILPLEFELTFSPPS
GEQADLSRPSECGGATNYCTVTPSGEVLPCHYFEGVVADSLRNRSFRDIWRNSRFLNYFRSIEIGDIQGYCHGCDWLSEC
RGGCKAANFSRGDLFQSNCHCWVVKENHQ
>Mature_429_residues
MHYPYFPFFLSKQDRDTFMASSPYGGFTVNKAGAAILEWSNGSRSMGEIVKKLQSRYVAESASIAAKTEQFIHDLAKKGY
VLIKEKPMRWFDAPPPQTIFWEITSQCNLQCLHCVVSAGQSTEPDLSTARCFELLDEWAALGVEEITFSGGEPLLREDFF
DLAAAAAKNNLSISLATNGTLVTREVAKKLKALNVDVQISLDGSTAEIYGRVRGRKEAFNDVVQGIRNTLAAGVNLTIGT
VVTKNNIDDIPEILKFTERSGVPYFRLIPFVPSGRGKHNRDLELDPLQIQTVSRELVEQRDRWSFTILPLEFELTFSPPS
GEQADLSRPSECGGATNYCTVTPSGEVLPCHYFEGVVADSLRNRSFRDIWRNSRFLNYFRSIEIGDIQGYCHGCDWLSEC
RGGCKAANFSRGDLFQSNCHCWVVKENHQ

Specific function: Together With Moac, Is Involved In The Conversion Of A Guanosine Derivative (Gxp) Into Molybdopterin Precursor Z. [C]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

Organism=Homo sapiens, GI22027536, Length=185, Percent_Identity=28.6486486486486, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI116734668, Length=178, Percent_Identity=28.6516853932584, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786999, Length=185, Percent_Identity=29.1891891891892, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17561210, Length=261, Percent_Identity=24.904214559387, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI32566329, Length=266, Percent_Identity=23.3082706766917, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI32566331, Length=268, Percent_Identity=24.2537313432836, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 48002; Mature: 48002

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYPYFPFFLSKQDRDTFMASSPYGGFTVNKAGAAILEWSNGSRSMGEIVKKLQSRYVAE
CCCCCCCCEECCCCCCCEEECCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
SASIAAKTEQFIHDLAKKGYVLIKEKPMRWFDAPPPQTIFWEITSQCNLQCLHCVVSAGQ
HHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCC
STEPDLSTARCFELLDEWAALGVEEITFSGGEPLLREDFFDLAAAAAKNNLSISLATNGT
CCCCCCHHHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCE
LVTREVAKKLKALNVDVQISLDGSTAEIYGRVRGRKEAFNDVVQGIRNTLAAGVNLTIGT
EEHHHHHHHHHHHCCEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEE
VVTKNNIDDIPEILKFTERSGVPYFRLIPFVPSGRGKHNRDLELDPLQIQTVSRELVEQR
EEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
DRWSFTILPLEFELTFSPPSGEQADLSRPSECGGATNYCTVTPSGEVLPCHYFEGVVADS
CCCEEEEEEEEEEEEECCCCCCCCCCCCCHHCCCCCCEEEECCCCCEEEHHHHHHHHHHH
LRNRSFRDIWRNSRFLNYFRSIEIGDIQGYCHGCDWLSECRGGCKAANFSRGDLFQSNCH
HCCCHHHHHHHCCHHHHHHHCCEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCE
CWVVKENHQ
EEEEECCCC
>Mature Secondary Structure
MHYPYFPFFLSKQDRDTFMASSPYGGFTVNKAGAAILEWSNGSRSMGEIVKKLQSRYVAE
CCCCCCCCEECCCCCCCEEECCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
SASIAAKTEQFIHDLAKKGYVLIKEKPMRWFDAPPPQTIFWEITSQCNLQCLHCVVSAGQ
HHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCC
STEPDLSTARCFELLDEWAALGVEEITFSGGEPLLREDFFDLAAAAAKNNLSISLATNGT
CCCCCCHHHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCE
LVTREVAKKLKALNVDVQISLDGSTAEIYGRVRGRKEAFNDVVQGIRNTLAAGVNLTIGT
EEHHHHHHHHHHHCCEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEE
VVTKNNIDDIPEILKFTERSGVPYFRLIPFVPSGRGKHNRDLELDPLQIQTVSRELVEQR
EEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
DRWSFTILPLEFELTFSPPSGEQADLSRPSECGGATNYCTVTPSGEVLPCHYFEGVVADS
CCCEEEEEEEEEEEEECCCCCCCCCCCCCHHCCCCCCEEEECCCCCEEEHHHHHHHHHHH
LRNRSFRDIWRNSRFLNYFRSIEIGDIQGYCHGCDWLSECRGGCKAANFSRGDLFQSNCH
HCCCHHHHHHHCCHHHHHHHCCEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCE
CWVVKENHQ
EEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA