Click here to switch to the map view.

The map label for this gene is recF [H]

Identifier: 119355860

GI number: 119355860

Start: 3303

End: 4400

Strand: Direct

Name: recF [H]

Synonym: Cpha266_0003

Alternate gene names: 119355860

Gene position: 3303-4400 (Clockwise)

Preceding gene: 119355859

Following gene: 119355861

Centisome position: 0.11

GC content: 34.52

Gene sequence:

>1098_bases
TTGAGGCTTACACGAATAACCTACAATAACTTCAGGAATTACAGAAAAATGACCTTTGAACCGAATGCAGGGATAACCCT
TCTTTATGGTTCAAATGGATCCGGAAAAACAAATATTCTTGAGGGCATTCATTATTGTGCTCTTACAAAAGGTTTTACGA
GTATTGCCGATAGTGACTGTATTTTTGATTCCAGTGATTATTATGCCCTGCAAAGTACTTGTTTGGGTGAAAATGGCAGT
GATATTGAAGTCAGAATATCGTTCTCAAGAGAGAAAGGCAAAACTCTTTTTGTTAATAATAACGAGATTAAAAAATTTTC
CAATCATGTTGGTACCATTCCCTGTATAACATTTTCTCCCCCTGAAATATCAATTGTCAGCGGATCTCCATCAGAAAGAA
GAAAATTCATAGACAATATTATTTGTCAATCTGATAAAAAATATTTAAAGGATCTCTTGACATATCGACGGGTTCTTCTT
CAGAGAAATGCTCTTCTTGCACAAATCAGTGAAAAAAAATCTTCTATAAATATGCTTCCGTATTGGTCGGAAAATCTTTC
GGTTCTGGCAGCTTCAATTGTTTTTAAACGCTTGGAATTTTTAGATAAATTCATTGATAACTTCAGAGATCTTTTTAAAA
AACTTTCTATTAACGAAGAACCTGGAATAGTTTATCGTTCAGTATTGGGCAGATATGATAACATCAGGAATATCGATGAA
CTTGCAGCTCTATATTATAGAAAATATGATGATAATCTTCGATATGAGTTATTGAGATCTCAAACATCCTGCGGACCTCA
CAGGGATGATCTTGAATTTTATATAAACGATAAAGAGATAAAAAAATACGCATCGCAGGGTCAGTTAAGGACTTTTCTAA
TCGGTCTCAAACTTGCTGTTTACGATTATCTTTTCGATACGACGCATGAAAAGCCTATATGTCTTCTTGACGACATTTTC
AGTGAACTTGATACGCAAAGAACGGAAAATATTCTCTCTATACTTCAAACACTCGGCCAGTCGATCATTACCTCTACGGT
CAGAAGAGATTCGGATATTATTTCAAGCGTTTCTGTACACGATTTAATCAACGATTAA

Upstream 100 bases:

>100_bases
AATTTCATCTATTCTTTCTTCATATTTTACTGAATATCGTATAGATTACCCAAAAATACGGTTGATGTTTTTTTTTAAGA
AATTACAAAAGGAATAATTA

Downstream 100 bases:

>100_bases
CTATAGCGTCATGTCAAGAACAAAGAGTCCGAGGATACTCTCATCCGTTGTTCAGGATGTCTGTAGAACTCTTGGTATGA
ACAAGGCTATTGATGATTTC

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MRLTRITYNNFRNYRKMTFEPNAGITLLYGSNGSGKTNILEGIHYCALTKGFTSIADSDCIFDSSDYYALQSTCLGENGS
DIEVRISFSREKGKTLFVNNNEIKKFSNHVGTIPCITFSPPEISIVSGSPSERRKFIDNIICQSDKKYLKDLLTYRRVLL
QRNALLAQISEKKSSINMLPYWSENLSVLAASIVFKRLEFLDKFIDNFRDLFKKLSINEEPGIVYRSVLGRYDNIRNIDE
LAALYYRKYDDNLRYELLRSQTSCGPHRDDLEFYINDKEIKKYASQGQLRTFLIGLKLAVYDYLFDTTHEKPICLLDDIF
SELDTQRTENILSILQTLGQSIITSTVRRDSDIISSVSVHDLIND

Sequences:

>Translated_365_residues
MRLTRITYNNFRNYRKMTFEPNAGITLLYGSNGSGKTNILEGIHYCALTKGFTSIADSDCIFDSSDYYALQSTCLGENGS
DIEVRISFSREKGKTLFVNNNEIKKFSNHVGTIPCITFSPPEISIVSGSPSERRKFIDNIICQSDKKYLKDLLTYRRVLL
QRNALLAQISEKKSSINMLPYWSENLSVLAASIVFKRLEFLDKFIDNFRDLFKKLSINEEPGIVYRSVLGRYDNIRNIDE
LAALYYRKYDDNLRYELLRSQTSCGPHRDDLEFYINDKEIKKYASQGQLRTFLIGLKLAVYDYLFDTTHEKPICLLDDIF
SELDTQRTENILSILQTLGQSIITSTVRRDSDIISSVSVHDLIND
>Mature_365_residues
MRLTRITYNNFRNYRKMTFEPNAGITLLYGSNGSGKTNILEGIHYCALTKGFTSIADSDCIFDSSDYYALQSTCLGENGS
DIEVRISFSREKGKTLFVNNNEIKKFSNHVGTIPCITFSPPEISIVSGSPSERRKFIDNIICQSDKKYLKDLLTYRRVLL
QRNALLAQISEKKSSINMLPYWSENLSVLAASIVFKRLEFLDKFIDNFRDLFKKLSINEEPGIVYRSVLGRYDNIRNIDE
LAALYYRKYDDNLRYELLRSQTSCGPHRDDLEFYINDKEIKKYASQGQLRTFLIGLKLAVYDYLFDTTHEKPICLLDDIF
SELDTQRTENILSILQTLGQSIITSTVRRDSDIISSVSVHDLIND

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=347, Percent_Identity=24.207492795389, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 42004; Mature: 42004

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00617 RECF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTRITYNNFRNYRKMTFEPNAGITLLYGSNGSGKTNILEGIHYCALTKGFTSIADSDC
CCEEEEEHHHHHCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
IFDSSDYYALQSTCLGENGSDIEVRISFSREKGKTLFVNNNEIKKFSNHVGTIPCITFSP
EECCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEECCHHHHHHCCCCCCEEEEECC
PEISIVSGSPSERRKFIDNIICQSDKKYLKDLLTYRRVLLQRNALLAQISEKKSSINMLP
CCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YWSENLSVLAASIVFKRLEFLDKFIDNFRDLFKKLSINEEPGIVYRSVLGRYDNIRNIDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHH
LAALYYRKYDDNLRYELLRSQTSCGPHRDDLEFYINDKEIKKYASQGQLRTFLIGLKLAV
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHHH
YDYLFDTTHEKPICLLDDIFSELDTQRTENILSILQTLGQSIITSTVRRDSDIISSVSVH
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLIND
HHHCC
>Mature Secondary Structure
MRLTRITYNNFRNYRKMTFEPNAGITLLYGSNGSGKTNILEGIHYCALTKGFTSIADSDC
CCEEEEEHHHHHCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
IFDSSDYYALQSTCLGENGSDIEVRISFSREKGKTLFVNNNEIKKFSNHVGTIPCITFSP
EECCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEECCHHHHHHCCCCCCEEEEECC
PEISIVSGSPSERRKFIDNIICQSDKKYLKDLLTYRRVLLQRNALLAQISEKKSSINMLP
CCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YWSENLSVLAASIVFKRLEFLDKFIDNFRDLFKKLSINEEPGIVYRSVLGRYDNIRNIDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHH
LAALYYRKYDDNLRYELLRSQTSCGPHRDDLEFYINDKEIKKYASQGQLRTFLIGLKLAV
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHHH
YDYLFDTTHEKPICLLDDIFSELDTQRTENILSILQTLGQSIITSTVRRDSDIISSVSVH
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLIND
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA