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The map label for this gene is dnaN [H]

Identifier: 119355859

GI number: 119355859

Start: 1895

End: 3025

Strand: Direct

Name: dnaN [H]

Synonym: Cpha266_0002

Alternate gene names: 119355859

Gene position: 1895-3025 (Clockwise)

Preceding gene: 119355858

Following gene: 119355860

Centisome position: 0.06

GC content: 41.38

Gene sequence:

>1131_bases
ATGAACATGAAATTCACCGCTCCAATTCGTCTCTTGCAGGATGCCGTTAACCGGGTAAGTCAGGCAATTCCGTCAAAAGC
AATCGATCCGCGTTTTGAAAATATCCATCTTGCTATTGAACCGGGAAAATTGACCCTTTTTGCCTCAGACGGCGAGATGT
CGATTACTGCAAAAAGTGAAATCAGTTCTGAAGACACAGGAAATATCAGTATCAAAGCAAGAACCCTTCAGGATTTTCTT
CGAAGCATGTATGATACCGAGGTCACCTTCTCTATTGAACGACAGGAGATCAGTGATCATGGGACAGTATCCATTTCAAC
CGATAAAGGCAAGTACAAGATCCCCTGTTTTTTTGAGAGCAAAATGGAGATCTATGAAAAAAATTATGTGATCAACCTGG
ATCTCCCCTCAGTAGAATTACAGTATCTTATTCAGAAAACAATTTTCGCCTGCAGCGTCGATGGTATGAGGCCGGCAATG
ATGGGTGTTCTTTTTGAACTGGAAAGCTCATCGATTACCGCTGTTTCAACTGACGGTCACAGACTTGTTCGTTGTAGAAA
AAGTACGGTGATAGAAGTAGAAGACAAGCAGAAAATTGTCGTTTCTGCGCGCGTTCTCTCTATTTTACAGAAGCTGCCTC
TTCATGAAACCGTTATAATTCAGATTGATCAGGATAAACGATTCGTTCGTTTCACACAAAATAATGTCGTTATCGATGCT
TCGCTGATCGTCGAACCATACCCTAATTACGAGGCAGTGATCCCTGTGATCCACGATAAGCAGGTAAGTTGTGATCGATC
CAATATCTACGATTCAGTAAAAAGAGTCGGAAGATTTTCAAGCGTTGGCGATATCAAGATGGTTTTAGCCGATTCGATTA
TAAAGGTAATGGCTGAAAATACCAACGAAGGAGAATCTGCCCAGGAGGAGTTGCCGTGTTCCTATACCGGCGAAGATATT
ACAATAGGTTTCAATGCGAAATTTATCGAGGCCGCTTTAGCGCATATTGACGAAAAAGAGATTATCATTGAATTAAAAAG
CCCTACGACAGCAGTAATATTTAAACCTGTCCAGGAAAAAGAAACCGGCAGTCTTATTATTCTTGTTATGCCTGTACGGA
TTAACGCATAA

Upstream 100 bases:

>100_bases
TTATTTTATACCGTTAAAATTGATATTTATTGTGTAGTCAAGAAATCAACACGCACAACAACAATGACAACAAATTATAT
TTAATAAATAGTTAATACTG

Downstream 100 bases:

>100_bases
TACTATCTTCAAGCGTTTGACTTCTGATGCATACAACTGCTTGATAAAAATGGAACTCGCAATACTGAATAAAGGTAAAT
CTTGAATTCACTATTGTGAG

Product: DNA polymerase III, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MNMKFTAPIRLLQDAVNRVSQAIPSKAIDPRFENIHLAIEPGKLTLFASDGEMSITAKSEISSEDTGNISIKARTLQDFL
RSMYDTEVTFSIERQEISDHGTVSISTDKGKYKIPCFFESKMEIYEKNYVINLDLPSVELQYLIQKTIFACSVDGMRPAM
MGVLFELESSSITAVSTDGHRLVRCRKSTVIEVEDKQKIVVSARVLSILQKLPLHETVIIQIDQDKRFVRFTQNNVVIDA
SLIVEPYPNYEAVIPVIHDKQVSCDRSNIYDSVKRVGRFSSVGDIKMVLADSIIKVMAENTNEGESAQEELPCSYTGEDI
TIGFNAKFIEAALAHIDEKEIIIELKSPTTAVIFKPVQEKETGSLIILVMPVRINA

Sequences:

>Translated_376_residues
MNMKFTAPIRLLQDAVNRVSQAIPSKAIDPRFENIHLAIEPGKLTLFASDGEMSITAKSEISSEDTGNISIKARTLQDFL
RSMYDTEVTFSIERQEISDHGTVSISTDKGKYKIPCFFESKMEIYEKNYVINLDLPSVELQYLIQKTIFACSVDGMRPAM
MGVLFELESSSITAVSTDGHRLVRCRKSTVIEVEDKQKIVVSARVLSILQKLPLHETVIIQIDQDKRFVRFTQNNVVIDA
SLIVEPYPNYEAVIPVIHDKQVSCDRSNIYDSVKRVGRFSSVGDIKMVLADSIIKVMAENTNEGESAQEELPCSYTGEDI
TIGFNAKFIEAALAHIDEKEIIIELKSPTTAVIFKPVQEKETGSLIILVMPVRINA
>Mature_376_residues
MNMKFTAPIRLLQDAVNRVSQAIPSKAIDPRFENIHLAIEPGKLTLFASDGEMSITAKSEISSEDTGNISIKARTLQDFL
RSMYDTEVTFSIERQEISDHGTVSISTDKGKYKIPCFFESKMEIYEKNYVINLDLPSVELQYLIQKTIFACSVDGMRPAM
MGVLFELESSSITAVSTDGHRLVRCRKSTVIEVEDKQKIVVSARVLSILQKLPLHETVIIQIDQDKRFVRFTQNNVVIDA
SLIVEPYPNYEAVIPVIHDKQVSCDRSNIYDSVKRVGRFSSVGDIKMVLADSIIKVMAENTNEGESAQEELPCSYTGEDI
TIGFNAKFIEAALAHIDEKEIIIELKSPTTAVIFKPVQEKETGSLIILVMPVRINA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=380, Percent_Identity=22.8947368421053, Blast_Score=98, Evalue=7e-22,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42103; Mature: 42103

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMKFTAPIRLLQDAVNRVSQAIPSKAIDPRFENIHLAIEPGKLTLFASDGEMSITAKSE
CCCEECHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCEEEEEECCCCEEEEECCC
ISSEDTGNISIKARTLQDFLRSMYDTEVTFSIERQEISDHGTVSISTDKGKYKIPCFFES
CCCCCCCEEEEEEHHHHHHHHHHCCCEEEEEEEHHHCCCCCEEEEEECCCCEEEEEEECC
KMEIYEKNYVINLDLPSVELQYLIQKTIFACSVDGMRPAMMGVLFELESSSITAVSTDGH
CHHEEECCEEEEECCCCHHHHHHHHHHHHEEECCCCCHHHEEEEEEECCCCEEEEECCCC
RLVRCRKSTVIEVEDKQKIVVSARVLSILQKLPLHETVIIQIDQDKRFVRFTQNNVVIDA
EEEEECCCCEEEECCCCEEEHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCEEEEE
SLIVEPYPNYEAVIPVIHDKQVSCDRSNIYDSVKRVGRFSSVGDIKMVLADSIIKVMAEN
EEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
TNEGESAQEELPCSYTGEDITIGFNAKFIEAALAHIDEKEIIIELKSPTTAVIFKPVQEK
CCCCCCHHHHCCCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCC
ETGSLIILVMPVRINA
CCCCEEEEEEEEEECC
>Mature Secondary Structure
MNMKFTAPIRLLQDAVNRVSQAIPSKAIDPRFENIHLAIEPGKLTLFASDGEMSITAKSE
CCCEECHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCEEEEEECCCCEEEEECCC
ISSEDTGNISIKARTLQDFLRSMYDTEVTFSIERQEISDHGTVSISTDKGKYKIPCFFES
CCCCCCCEEEEEEHHHHHHHHHHCCCEEEEEEEHHHCCCCCEEEEEECCCCEEEEEEECC
KMEIYEKNYVINLDLPSVELQYLIQKTIFACSVDGMRPAMMGVLFELESSSITAVSTDGH
CHHEEECCEEEEECCCCHHHHHHHHHHHHEEECCCCCHHHEEEEEEECCCCEEEEECCCC
RLVRCRKSTVIEVEDKQKIVVSARVLSILQKLPLHETVIIQIDQDKRFVRFTQNNVVIDA
EEEEECCCCEEEECCCCEEEHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCEEEEE
SLIVEPYPNYEAVIPVIHDKQVSCDRSNIYDSVKRVGRFSSVGDIKMVLADSIIKVMAEN
EEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
TNEGESAQEELPCSYTGEDITIGFNAKFIEAALAHIDEKEIIIELKSPTTAVIFKPVQEK
CCCCCCHHHHCCCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCC
ETGSLIILVMPVRINA
CCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]